Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,861,213 A→C 22.7% V248V (GTT→GTG)  U712_14765 ← Uncharacterized protein YtxD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,861,2130AC22.7% 77.9 / 2.6 41V248V (GTT→GTG) U712_14765Uncharacterized protein YtxD
Reads supporting (aligned to +/- strand):  ref base A (23/8);  new base C (2/8);  total (25/16)
Fisher's exact test for biased strand distribution p-value = 6.62e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGGATGAAGATTGACTGTTTTTTCCGCTTCCATTCCTTCTTTCAAAACGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCTTAGCCGCGATCGGGTTGAAGACCA  >  CP006881/2861079‑2861349
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aGCTGGATGATGATTGACTGTTTTTTCCGCTTCCATTCCTTCTTTCAAAACGTTCTCTTCTAAGCTTCATGTACATATCCCTTTGTTGTATTTTCTTGTTTTGGCTGCTTCTGGCATTCTTCCCGTGAGCTGAAAACGa                                                                                                                                      >  1:115487/1‑139 (MQ=255)
aGCTGGATGAAGATTGACTGTTTTTTCCGCTTCCATTCCTTCTTTCAAAACGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGa                                                                                                                                      >  3:270588/1‑139 (MQ=255)
     gATGAAGATTGACTGTTTTTTCCGCTTCCATTCCTTCTTTCAAAACGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGc                                                                                                                                 >  7:199963/1‑139 (MQ=255)
      aTGAAGATTGACTGTTTTTTCCGCTTCCATTCCTTCTTTCAAAACGATCTCTTCTAAGCTTCATGTACAGATCCCTGTTTTGTTATTACTTGTTTTGGCTGATTCTGCCGTACTTCCAGTGAGCTGAACACGACCAGCt                                                                                                                                >  7:141822/1‑139 (MQ=255)
        gAAGATTGACTGTTTTTTCCGCTTCCATTCCTTCTTTCAAAACGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTg                                                                                                                               <  8:199963/138‑1 (MQ=255)
                aCTGTTTTTTCCGCTTCCATTCCTTCTTTCAAAACGTTCTCTTCTAAGCTTCTTGTACAGATCCCTTTTTTGTTTTTAATTGTTTTGGCTGGTTCTGCCTTTCTTCCCGTGAGCTTAAAACGACCAGCTGGCTTTCaa                                                                                                                       <  7:328721/138‑1 (MQ=255)
                   gTTTTTTCCGCTTCCATTCCTTCTTTCAACACGTTCTCTTCTAAGCTTCATGTACAGCTCACTAATTTGTTTTTAATTGTTTTTGCTGCTTCGGCCATTCTTCCCGTGAGCTGAACACGCCCAGCTGGCTTACCAGGtt                                                                                                                   >  3:305668/1‑139 (MQ=255)
                                ccATTCCTTCTTTCAAAACGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGtttttt                                                                                                      <  8:103362/139‑1 (MQ=255)
                                        tcttTCAAAACGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGAtt                                                                                               >  4:121466/1‑138 (MQ=255)
                                          tttCAAAACGTTCTCTTCTAAGCTTCATGAACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTACCCGTTAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGaa                                                                                            <  7:266006/139‑1 (MQ=255)
                                            tCAAAACGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCGGCTTCTGCAATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAac                                                                                           <  3:91292/138‑1 (MQ=255)
                                            acAAATCGTTCTGTGCTAAGCTTCATGTACCGCTCCCTTTTTTGTTTTTACTTGTTTGGGCGGCTTCTGCCATTCTTCCGGTGAGCTCAACACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGaa                                                                                            <  8:141822/136‑1 (MQ=255)
                                              aaaaCGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACAcc                                                                                        >  5:301431/1‑139 (MQ=255)
                                                aaCGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGa                                                                                      >  3:162712/1‑139 (MQ=255)
                                                  cGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATa                                                                                    <  3:195306/139‑1 (MQ=255)
                                                           ctACGCTTCATGAACAGCTCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAACACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTcc                                                                           <  4:56966/139‑1 (MQ=255)
                                                           ctAAGCTTATTGAAAATATCCCTTTTTTGTTTTTACTTGTTGTGCGTGCTTCTGACATTCTTCCCGTGAGCTGAACACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTc                                                                            <  7:160528/138‑1 (MQ=255)
                                                           ctAAGCTGAATGAACAGATCCATTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCAATTCTTCCCTTCAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCtt                                                                             <  8:188156/137‑1 (MQ=255)
                                                            tAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTACGGATTGAACACCGATAATCCCTTcaa                                                                          >  1:325246/1‑137 (MQ=255)
                                                              aGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTccac                                                                         >  7:133503/1‑138 (MQ=255)
                                                                   caggTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAACACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTa                                                                    <  4:312800/135‑1 (MQ=255)
                                                                   cATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGCCCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTAc                                                                   >  7:165295/1‑139 (MQ=255)
                                                                   cATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTcc                                                                                                    >  8:110637/1‑106 (MQ=255)
                                                                   cATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTcc                                                                                                    <  7:110637/106‑1 (MQ=255)
                                                                   cATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTAc                                                                   >  5:290073/1‑139 (MQ=255)
                                                                   cATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTAc                                                                   >  6:4715/1‑139 (MQ=255)
                                                                   cATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTAc                                                                   >  7:188156/1‑139 (MQ=255)
                                                                   cATGTACAGATCACTTTTTTGTTTTTACTATTTTTGGCTGCTTCTGACATACTTCCCGTGAGCTGAAAACTCCCATCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGAGTGAACTCCGATAAACCCTTCAACCATTAc                                                                   >  2:341462/1‑139 (MQ=255)
                                                                           gATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTtga                                                           >  8:208461/1‑139 (MQ=255)
                                                                            aTCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAACACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTtgat                                                          <  5:187557/139‑1 (MQ=255)
                                                                                   ttttGTTTTTACTTGTTTTGGCGGCTTCTGCCATTCTTCCCGGCACCTTAACACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAAt                                                    <  3:121466/138‑1 (MQ=255)
                                                                                                tGTTTTGTCTGCTTCTGCCTATCTGCCCGTCAGAGCAACACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGtt                                       <  6:15978/138‑1 (MQ=255)
                                                                                                      ggCTGCTTCTGCCATTCTTCCCGTTAGCTGAACACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTctct                                 <  8:55233/138‑1 (MQ=255)
                                                                                                            ttCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCt                          >  1:359534/1‑139 (MQ=255)
                                                                                                            ttCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCt                          >  1:354191/1‑139 (MQ=255)
                                                                                                                     tcttcCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCTTATCCGCGa                 >  3:317384/1‑139 (MQ=255)
                                                                                                                     tcttcCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCTTAGCCGCGa                 >  7:290096/1‑139 (MQ=255)
                                                                                                                     tcttcCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACATGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCTTAGCCGCGa                 >  5:376817/1‑139 (MQ=255)
                                                                                                                     tcttaCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCAGGATGGCACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTAACTTTCTGTATACTCTTCAAGCTTAGCCGCGa                 >  5:4186/1‑139 (MQ=255)
                                                                                                                         cccGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCTTAGCCGCGATCgg             >  4:54590/1‑139 (MQ=255)
                                                                                                                               aGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCAGTACCTGCTTGATCAAAATATCACTATCTGTTTTCTCTTCAACCTTAGCCGCGATCGGGTTg         >  6:25083/1‑137 (MQ=255)
                                                                                                                                 cttaaaaCGACCAGCTGGCTTTCAAGGTTGCGCGTGGTTTTTCCGGATTGAACACCGATACTCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCTTAGCCGCGATCGGGTTGAAGa     <  2:235557/136‑1 (MQ=255)
                                                                                                                                    aaaaCGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCTAGCTTAGCCGCGATCGGGTTGAAGACCa  >  1:355589/1‑139 (MQ=255)
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AGCTGGATGAAGATTGACTGTTTTTTCCGCTTCCATTCCTTCTTTCAAAACGTTCTCTTCTAAGCTTCATGTACAGATCCCTTTTTTGTTTTTACTTGTTTTGGCTGCTTCTGCCATTCTTCCCGTGAGCTGAAAACGACCAGCTGGCTTTCAAGGTTGCGCGGGTTTTTTCCGGATTGAACACCGATAATCCCTTCCACCATTACCTGCTTGATGAAAATTTCACTTTCTGTTTTCTCTTCAAGCTTAGCCGCGATCGGGTTGAAGACCA  >  CP006881/2861079‑2861349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑