Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,476,692 G→T 21.5% H19N (CAT→AAT)  U712_17855 ← putative undecaprenyl‑phosphate galactose phosphotransferase‑like Protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,476,6920GT21.5% 69.5 / 3.3 49H19N (CAT→AAT) U712_17855putative undecaprenyl‑phosphate galactose phosphotransferase‑like Protein
Reads supporting (aligned to +/- strand):  ref base G (24/13);  new base T (4/8);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 9.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCTCCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACGGATGTTAATTGGATGTAAA  >  CP006881/3476555‑3476829
                                                                                                                                         |                                                                                                                                         
cGTCTCCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGt                                                                                                                                          <  1:260688/139‑1 (MQ=255)
    tcCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTGGTTTAAGAAAATTTAAAAGAAAGTAttt                                                                                                                                      >  2:260688/1‑139 (MQ=255)
             aTCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAAACCAATGAGCGCAAACCAAATATACATTACTCGCTTTATTGCCAAATAACGAGCCGTCTTTTGAGATAAAGTAAAATAATGTATTTTTTGAAATg                                                                                                                             >  6:118827/1‑139 (MQ=255)
             aTCAAAATTGAAAATACAGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATAACGCGCTTTATTGCCAAATAACGAGCCGTCATTTACTAATATGTAAAAAAAATTAATAGTTGaaat                                                                                                                              >  6:8663/1‑136 (MQ=255)
              tCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACt                                                                                                                              >  7:84126/1‑137 (MQ=255)
                  aaTTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTGAGATAATGTAAAAGAATGTATTTGTTTAACAGAGAAt                                                                                                                        >  3:43889/1‑139 (MQ=255)
                  aaTTGAAAATACAGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCATTTGACATAATGTAAAAGAAAGTAATTTTTGAAAAGAGAAt                                                                                                                        >  5:116520/1‑139 (MQ=255)
                       aaaaTACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCAc                                                                                                                    >  1:34111/1‑138 (MQ=255)
                            aCCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCGTTTCAGATAATGTAAAATAATGTATTTGTTGTACTGAGAATTCACGACTca                                                                                                              >  7:390194/1‑139 (MQ=255)
                                      ttGCCACCGCAATCTCAAATCCAATGCCCCAAAACCAAATATCCTTAACTCGTTTTATCCCCAAAAACCGCGCCGTCTTTTCATATAATGTAAAACAATTTTTTTGTTCAAGGGAAAATTCACGACTCACATTCATGCt                                                                                                    <  8:278572/139‑1 (MQ=255)
                                      tgtgCAGCCCAATCGCCAAACCAATGAGCGAAAACCAAAAAACCATTACGCGCTTTATTCCCAAAAACCGCGCCGTCTTTTCAGATAATGAAAAAGAATTTATTTGTTCAACTGAGAATTCACGACTCACATTCATGCt                                                                                                    <  2:99321/136‑1 (MQ=255)
                                      tgggCAGCCCAATCCCAAATCAAATCAGCGAAAACAAAAAACCAATTCCGCGCTTTATTGCCAAATACCGAGCCGTCTTTTCTGATAATTTAAAAGAATGTATTTGTTGAACTTATAATCCACGACTCACATTCATGCt                                                                                                    <  6:357462/137‑1 (MQ=255)
                                       tGGAAGCGCAATCGCAAATCAAATGAGCCCAAACCAAATATCCATTACGCGCTTTATTGCAAAATAACGAGCCTTATTTTCAGATATTTTAAAATAATGTATTTGTTGAACTGATAATTCACGACTCACATTCATGCt                                                                                                    <  2:34111/138‑1 (MQ=255)
                                             gtcAATCGCAAATCCAACGAGCGCAAACCAAATATCCATTAGGCGCTTTATTGCAAAATAACGACCCGTCTTTTCAGATAATTTAAAAGAATGTATTTGTTGAAGTTACATACCACGACTCACATTCATGCTTTtct                                                                                               <  6:154119/135‑1 (MQ=255)
                                             cgcAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGt                                                                                                                                          <  3:95913/94‑1 (MQ=255)
                                             cgcAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGt                                                                                                                                          >  4:95913/1‑94 (MQ=255)
                                             cgcAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTTTTTGTTGAACTGAGAATTCACGACTCACATTCATGCGTTtctgt                                                                                             >  3:55524/1‑137 (MQ=255)
                                             cgcAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGCCTCACATTCATGCTTTTctct                                                                                             >  1:15422/1‑139 (MQ=255)
                                             cgcAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTAAGATAATGTAAAATAATGTATTTGTTTAACAGAGAATTCACGACTCACATTCATGCTTTTatct                                                                                             >  8:291384/1‑139 (MQ=255)
                                             cgcAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCGTTATTGCCAAATAACGAGCCGTGGTTTGTGATTATTTTAAATAATTTATTTGTTTTAGTGAGAATTAACGACTCACATTGATGCttttatag                                                                                             >  6:108152/1‑135 (MQ=255)
                                               cAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTAAGATAATGTAAAAGAATGTATTTGTTTAACTGAGAATTCACGACTCACATTCATGCttttgagtg                                                                                            >  4:343270/1‑133 (MQ=255)
                                                    gCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGATTTATTGCCAAATACCGAGCCGTCTTTACATATAATTTAAAAGAATGTTTTTGTTGAACTGATAATTCACGACTCACATTCATGCTTTTCTCTGcactca                                                                                      <  3:333917/139‑1 (MQ=255)
                                                     ccaacccaatCACCCCAAACCAAATACCCATACCGCGCTATATCCCCAAAAACCGCCCCGTATTTTCAGATAATTAAAAACAATGTATGTGTTCAACTCCCACACCACGACTCACATTCATGCTTTTCTCTGcactcac                                                                                     <  2:304465/139‑1 (MQ=255)
                                                                        caaaaTAACCATTCAGCGATTTATTCCAAAAAAACGCCCGTTCTTATAATAAATTGTAAAACATTTTTTTTGTTCACCTGACAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGAtt                                                                  <  7:291384/137‑1 (MQ=255)
                                                                              atCCATTACGCGCTTTATTGCAAAATACCGCGCCGTCTTTTCAGATAATTTAAAACAATGTATTTGTTGAACTGACAATCCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGAtttgtttg                                                            <  4:55524/139‑1 (MQ=255)
                                                                               tacATTCCGCGCTTTTTTGAAAAAACCAGCGCAGTATTTTCATATAATTTAAAAGAATGTATTTGTTTAAACTATAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGAtttgtttg                                                            <  2:257618/136‑1 (MQ=255)
                                                                                   ttACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCCCGATTTGTTTGGGATg                                                       >  3:358153/1‑139 (MQ=255)
                                                                                   ttACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATg                                                       >  8:250459/1‑139 (MQ=255)
                                                                                   ttACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTAAGAAAATGTTAAAGAATGTTTTTGTTGAACTGTGAATTCACGACTCCCATTCATGCGTTTCTCTGCACGCACGGACACCCACCTAACGATTTGTTTGGGATg                                                       >  2:290417/1‑139 (MQ=255)
                                                                                        cgcTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATTTAAAATAATTTATTTGTTGAACTGAGAATTAACGACTCACATTAATGGTTTTCTCTGCAAGCACTGACACACACCTCACGATTTTTTTGGGATTTTttg                                                  >  6:211614/1‑139 (MQ=255)
                                                                                         gcTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTTAACTGAGAATTAACGACTCACATTCATGCTTTTCTCTGCACTCCCGGACACACACCTCCCGATTTGTTTGGGATGttttta                                                 >  4:288769/1‑135 (MQ=255)
                                                                                         gcTTTATTCCCAAAAACCGCGCCGTCTTTACACAACATGAAAAACAATGTATTTGTTCAACTCACAATACACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTatga                                                 <  7:96985/139‑1 (MQ=255)
                                                                                           tttATTGCCAAATAACGAGCCGTCTTTTGAGATAATGTAAAAGAATTTATTTGTTGAACTGAGAATTAACGACTCACAATCATGCTTTTCTCTGCACTCACAGCCACCCACCTCACCCTTTGTTTTGGATGTTattatg                                               >  4:267517/1‑139 (MQ=255)
                                                                                              attccaaaaTAACGAGCGGTTTTTAAATATATTTTAAAATATTTTTTTTGTTTAACTGAAAATACACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGAc                                             <  3:288769/132‑1 (MQ=255)
                                                                                                  caaaaaaaCGCGCCGTCTTTTCATATAATGTAAAATATTTATTTTGTTGAAATAATAAGACACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACTATTTGTTTGGTATTTTATGATGACCTATg                                        <  5:118827/137‑1 (MQ=255)
                                                                                                     aaTAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGCCTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGAGTTGTTTGGGATGTTATGATGCCCTATGc                                       >  4:83756/1‑137 (MQ=255)
                                                                                                       aaaCGAGCCGTCTTTTCATATAATGAAAAACAATTTATTTGTTCAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCcagt                                   <  2:15422/138‑1 (MQ=255)
                                                                                                             gCCGTGTTTTGAGATAATGTAAAAGAATGTTTTTGTTGAACTGAGAATTCACGCCGCACATTCATGGGTTTCTCTGCACTCCCTGACCCACACCGCACGAGTTGTTTGGGATGTTTTGATGGCCTATGCCAGTCAGttt                             >  6:146609/1‑139 (MQ=255)
                                                                                                                      taatataataTAAAATAATTTATTTGTGAACCTCATAATCCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACg                     <  6:61511/134‑1 (MQ=255)
                                                                                                                      tCATATAATTTAAAATTATTTATTTGTTTAACTGAGAATACACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACg                     <  4:371464/138‑1 (MQ=255)
                                                                                                                      acataTAATGTAAAAGAATGTATTTGTTTACCTGACAATCCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACg                     <  8:390194/134‑1 (MQ=255)
                                                                                                                        aGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGCCTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACGGAt                  >  2:324139/1‑139 (MQ=255)
                                                                                                                           tAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACGGATGt                >  5:61511/1‑138 (MQ=255)
                                                                                                                           tAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACGGATGt                >  3:371464/1‑138 (MQ=255)
                                                                                                                                        tGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTa                                          <  7:371774/99‑1 (MQ=255)
                                                                                                                                        tGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTa                                          >  8:371774/1‑99 (MQ=255)
                                                                                                                                        tGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCcagt                                   <  2:212087/106‑1 (MQ=255)
                                                                                                                                        tGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCcagt                                   >  1:212087/1‑106 (MQ=255)
                                                                                                                                        tGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACGGATGTTAATTGGATGTaaa  >  2:329652/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CGTCTCCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCACTGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAGCAGACGGATGTTAATTGGATGTAAA  >  CP006881/3476555‑3476829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑