Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,568,212 T→A 5.3% F291Y (TTC→TAC)  U712_18310 → putative MFS‑type transporter ywoG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,568,2120TA 5.3% 245.0 / 3.3 76F291Y (TTC→TAC) U712_18310putative MFS‑type transporter ywoG
Reads supporting (aligned to +/- strand):  ref base T (36/36);  new base A (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

ATATTTCTTTGTTTGTTTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGCTCCGGTTTTGCGACGGCAACGTT  >  CP006881/3568075‑3568347
                                                                                                                                         |                                                                                                                                       
atatTTCTTTGTTTGTTTTGCGGTGACAATGATGATTGCACTCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCAtt                                                                                                                                         >  4:203506/1‑138 (MQ=255)
 tatTTCTTTGTTTGTTTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTTTCTGACTCTGCATGCTGTCCTTca                                                                                                                                       <  3:137865/139‑1 (MQ=255)
  atTTCTTTGTTTGTTTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAATTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTca                                                                                                                                       <  1:225749/138‑1 (MQ=255)
   tttCTTTGTTTGTTTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTcaca                                                                                                                                     <  6:69760/139‑1 (MQ=255)
   tttCTTTGTTTGTTTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTcaca                                                                                                                                     <  1:23360/139‑1 (MQ=255)
     tCTTTGTTTGTTTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTcacac                                                                                                                                    >  1:167288/1‑138 (MQ=255)
         tgtttgttTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAg                                                                                                                                <  8:94715/138‑1 (MQ=255)
         tgtttgttTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAg                                                                                                                                <  7:262388/138‑1 (MQ=255)
         tgtttgttTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAg                                                                                                                                <  3:380634/138‑1 (MQ=255)
         tgtttgttTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGc                                                                                                                               >  1:121197/1‑139 (MQ=255)
             tgtttTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCt                                                                                                                           >  6:132873/1‑139 (MQ=255)
                       tgtcattgatgaTTGCACGCCCGTGAACAGGGAAATTGTTCGACAAAGTCGGACCGTGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCt                                                                                                                 <  2:189984/133‑1 (MQ=255)
                       tGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCt                                                                                                                 <  1:208870/139‑1 (MQ=255)
                         actatgatgATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGc                                                                                                               >  1:189984/4‑139 (MQ=255)
                         aCAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCt                                                                                                                 <  8:176263/137‑1 (MQ=255)
                         aCAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCt                                                                                                                 >  7:176263/1‑137 (MQ=255)
                                   ttGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCTATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCgg                                                                                                                             <  8:267583/115‑1 (MQ=255)
                                   ttGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAg                                                                                                                                <  6:170116/112‑1 (MQ=255)
                                   ttGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAg                                                                                                                                >  5:170116/1‑112 (MQ=255)
                                   ttGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCgg                                                                                                                             >  7:267583/1‑115 (MQ=255)
                                   ttGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGc                                                                                                     >  8:57172/1‑139 (MQ=255)
                                     gCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCgg                                                                                                   >  6:1149/1‑139 (MQ=255)
                                         gCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACTCATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTAt                                                                                               <  6:182732/139‑1 (MQ=255)
                                         gCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTAt                                                                                               <  2:199540/139‑1 (MQ=255)
                                         gCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTAt                                                                                               <  2:167288/139‑1 (MQ=255)
                                           ccGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCg                                                                                             >  6:300380/1‑139 (MQ=255)
                                           ccGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCg                                                                                             <  5:332502/139‑1 (MQ=255)
                                              ttCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTc                                                                                          >  1:66107/1‑139 (MQ=255)
                                              ttCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTc                                                                                          >  1:226348/1‑139 (MQ=255)
                                                   aGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGc                                                                                     >  4:68790/1‑139 (MQ=255)
                                                     ggAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTa                                                                                   >  6:373495/1‑139 (MQ=255)
                                                     ggAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTACACACATAg                                                                                                                                <  7:231608/94‑1 (MQ=255)
                                                     ggAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTACACACATAg                                                                                                                                >  8:237274/1‑94 (MQ=255)
                                                     ggAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTACACACATAg                                                                                                                                >  8:231608/1‑94 (MQ=255)
                                                     ggAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTACACACATAg                                                                                                                                <  7:237274/94‑1 (MQ=255)
                                                          ttGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGAt                                                                              <  1:262374/139‑1 (MQ=255)
                                                             ttCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCaa                                                                           <  6:247948/139‑1 (MQ=255)
                                                               cGACAAAGTCGGACCGGGCATTGTCTTTTTTCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACAAATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAAt                                                                          <  1:26320/138‑1 (MQ=255)
                                                                   aaaGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTc                                                                     <  6:66742/139‑1 (MQ=255)
                                                                     aGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAg                                                                                                                                >  7:137923/1‑78 (MQ=255)
                                                                     aGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAg                                                                                                                                <  8:137923/78‑1 (MQ=255)
                                                                     aGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCt                                                                   <  5:373495/139‑1 (MQ=255)
                                                                               ggCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCg                                                                        <  5:248625/124‑1 (MQ=255)
                                                                               ggCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCg                                                                        >  6:248625/1‑124 (MQ=255)
                                                                               ggCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCaaa                                                         >  5:213856/1‑139 (MQ=255)
                                                                                     gTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCtt                                                                                                                   >  5:158840/1‑75 (MQ=255)
                                                                                     gTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCtt                                                                                                                   <  6:158840/75‑1 (MQ=255)
                                                                                     gTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTgg                                                   >  5:397777/1‑139 (MQ=255)
                                                                                      tCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTgg                                                   <  4:286995/138‑1 (MQ=255)
                                                                                      gcATTTATCCATCAATCCTGATTTTCTCTGTCGGACGCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGGTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTgg                                                   <  2:97758/137‑1 (MQ=255)
                                                                                         tttATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGt                                                                       >  7:157198/1‑115 (MQ=255)
                                                                                         tttATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGt                                                                       <  8:157198/115‑1 (MQ=255)
                                                                                                aTCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGAt                                                                              <  8:315548/101‑1 (MQ=255)
                                                                                                aTCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGAt                                                                              >  7:315548/1‑101 (MQ=255)
                                                                                                  cAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCtt                                                                                                                          <  6:234468/55‑1 (MQ=255)
                                                                                                  cAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCtt                                                                                                                          >  5:234468/1‑55 (MQ=255)
                                                                                                          aTTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTc                              >  3:223818/1‑139 (MQ=255)
                                                                                                             ttCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCAc                            <  7:57172/138‑1 (MQ=255)
                                                                                                               ctctGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGc                         >  8:268667/1‑139 (MQ=255)
                                                                                                               ctctGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGc                         >  8:135728/1‑139 (MQ=255)
                                                                                                                    tCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAAt                                                                          <  8:326206/85‑1 (MQ=255)
                                                                                                                    tCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAAt                                                                          >  5:177809/1‑85 (MQ=255)
                                                                                                                    tCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAAt                                                                          >  7:326206/1‑85 (MQ=255)
                                                                                                                    tCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAAt                                                                          <  6:177809/85‑1 (MQ=255)
                                                                                                                            tGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGCTCCGGTTTTGCGa            >  3:335376/1‑139 (MQ=255)
                                                                                                                            tGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGCTCCGGTTTTGCGa            >  4:153604/1‑139 (MQ=255)
                                                                                                                            tGCATGCTGTCATTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCATTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCAACGCTCCGGTATTGCGa            >  1:70336/1‑139 (MQ=255)
                                                                                                                              cATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCAc                            <  2:211808/121‑1 (MQ=255)
                                                                                                                              cATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCAc                            >  1:211808/1‑121 (MQ=255)
                                                                                                                              cATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGCTCCGGTTTTGCGACg          >  7:358849/1‑139 (MQ=255)
                                                                                                                                  cTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGCTCCGGTTTTGCGACGGCaa      <  5:1149/139‑1 (MQ=255)
                                                                                                                                    gTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTa                                                                                   <  7:311651/60‑1 (MQ=255)
                                                                                                                                    gTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTa                                                                                   >  8:311651/1‑60 (MQ=255)
                                                                                                                                     tCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGCTCCGGTTTTGCGACGGCAACGt   >  7:285620/1‑139 (MQ=255)
                                                                                                                                      ccTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGAt                                                                              <  8:184731/63‑1 (MQ=255)
                                                                                                                                      ccTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGAt                                                                              >  7:184731/1‑63 (MQ=255)
                                                                                                                                      ccTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGCTCCGGTTTTGCGACGGCAACGtt  >  2:182775/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATATTTCTTTGTTTGTTTTGCGGTGACAATGATGATTGCACGCCCGTTCACAGGGAAATTGTTCGACAAAGTCGGACCGGGCATTGTCATTTATCCATCAATCCTGATTTTCTCTGTCGGACTCTGCATGCTGTCCTTCACACATAGCGGCTTAATGCTTCTGCTTTCAGGGGCGGTTATCGGTCTTGGCTACGGATCAATCGTTCCTTGCATGCAAACATTGGCGATTCAGAAATCTCCGGCTCACCGCTCCGGTTTTGCGACGGCAACGTT  >  CP006881/3568075‑3568347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑