Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,669,015 A→T 5.1% E448V (GAA→GTA)  U712_18900 → Penicillin‑binding protein 2D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,669,0150AT 5.1% 251.9 / 3.9 78E448V (GAA→GTA) U712_18900Penicillin‑binding protein 2D
Reads supporting (aligned to +/- strand):  ref base A (37/37);  new base T (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

TTCTCCAAGCAACTATAACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTATGCCATGCTCGCAAATGGCGGAAAGAAAATCGAACCGACTTTTATTTCAA  >  CP006881/3668878‑3669152
                                                                                                                                         |                                                                                                                                         
ttCTCCAAGCAACTATTACGGATTCTATGCCAACATGACGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTTAGCTGTGAAAACGCATCTTTTTCTCGGCACGAACTAACTTGTATAGTCTGCATAGTaa                                                                                                                                          <  6:374259/139‑1 (MQ=255)
     cAAGCAACTATAACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACATGTAAAGACGCAAAAGGAATTCgg                                                                                                                                     >  7:274914/1‑139 (MQ=255)
      aaGCAACTATAACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCgg                                                                                                                                     >  2:287031/1‑138 (MQ=255)
       aGCAACTATAACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCa                                                                                                                                   >  5:318478/1‑139 (MQ=255)
       aGCAACTATAACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCa                                                                                                                                   >  3:102298/1‑139 (MQ=255)
             tataACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCg                                                                                                                             >  8:49623/1‑139 (MQ=255)
               taACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGcc                                                                                                                           <  5:151015/139‑1 (MQ=255)
               taACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGcc                                                                                                                           <  5:314710/139‑1 (MQ=255)
               taACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGcc                                                                                                                           <  3:212208/139‑1 (MQ=255)
               taACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGcc                                                                                                                           <  1:328564/139‑1 (MQ=255)
               taACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGcc                                                                                                                           <  1:287031/139‑1 (MQ=255)
                      tACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCTCCCATCtt                                                                                                                     >  7:26239/1‑138 (MQ=255)
                      tACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCGTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATcgt                                                                                                                     >  2:398306/1‑136 (MQ=255)
                       aCTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTc                                                                                                                    >  8:309058/1‑138 (MQ=255)
                       aCTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTc                                                                                                                    >  7:202540/1‑138 (MQ=255)
                       aCTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTc                                                                                                                    >  1:254401/1‑138 (MQ=255)
                       aCTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTc                                                                                                                    >  3:153924/1‑138 (MQ=255)
                       aCTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTc                                                                                                                    >  1:188983/1‑138 (MQ=255)
                       aCTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTc                                                                                                                    >  1:133035/1‑138 (MQ=255)
                       aCTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTc                                                                                                                    >  1:167240/1‑138 (MQ=255)
                       aCTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCGTTCCGCCAATATTTACGCTGGGAAAACGCATCTTTTTCTCGGCCCGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTTCCGCCCATCTTCa                                                                                                                   >  2:394169/1‑139 (MQ=255)
                           tGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAAtt                                                                                                                                        <  7:402711/114‑1 (MQ=255)
                           tGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAAtt                                                                                                                                        >  8:402711/1‑114 (MQ=255)
                           tGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAg                                                                                                                 <  7:49623/137‑1 (MQ=255)
                           tGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAg                                                                                                                 <  6:134954/137‑1 (MQ=255)
                           tGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCa                                                                                                               <  7:148967/139‑1 (MQ=255)
                           tGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATATACGCTGTCAAAACGCATCTTTTTCTCGGCACGATAAAACTTGTAAAGACTGCAAAGGAATTCGGCATTTCGGCCAATCATAAAGcc                                                                                                               >  8:308905/1‑138 (MQ=255)
                             ccAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCACGCACt                                                                                                             >  4:118568/1‑139 (MQ=255)
                                acaaGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGcc                                                                                                          >  1:341381/1‑139 (MQ=255)
                                acaaGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATGCGGCATTACCGCCCATCTTCAAGCACTGcc                                                                                                          <  6:278152/139‑1 (MQ=255)
                                 caaGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGcc                                                                                                          >  4:112869/1‑138 (MQ=255)
                                 caaGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCg                                                                                                         >  8:18697/1‑139 (MQ=255)
                                   aGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTc                                                                                                       >  5:254488/1‑139 (MQ=255)
                                   aGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACCCACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCCTCTTCAAGCACTTCCGTc                                                                                                       >  2:130720/1‑139 (MQ=255)
                                       gATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTg                                                                                                   <  2:341381/139‑1 (MQ=255)
                                        aTTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTgg                                                                                                  >  1:133375/1‑139 (MQ=255)
                                              cTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCg                                                                                            <  2:133375/139‑1 (MQ=255)
                                                  tGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCAc                                                                                        >  5:267022/1‑139 (MQ=255)
                                                    cAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCAc                                                                                        <  3:186131/137‑1 (MQ=255)
                                                    cAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCg                                                                                      <  6:267022/139‑1 (MQ=255)
                                                              cgctTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACg                                                                            <  7:309058/139‑1 (MQ=255)
                                                              cgctTTCCGACAATATATACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACg                                                                            <  6:112388/139‑1 (MQ=255)
                                                                   tCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGt                                                                           <  6:190176/135‑1 (MQ=255)
                                                                   tCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGt                                                                           >  7:141887/1‑135 (MQ=255)
                                                                   tCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGt                                                                           <  8:141887/135‑1 (MQ=255)
                                                                   tCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGt                                                                           >  5:190176/1‑135 (MQ=255)
                                                                         atatttttCGCTGTGACTACGCATCTTTATCTCGGCACGAACAAACTTGTACAGACGGCAGAGGAATTCGGCATAACCGCCCATGTTCAAGCACAGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTgaga                                                                 <  7:308905/135‑1 (MQ=255)
                                                                         aaTATTTCCGCTGTGACAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCACAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGACCCCGTACGTCCGATTgaga                                                                 <  6:279097/139‑1 (MQ=255)
                                                                               tACGCTGTTAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  5:244025/138‑1 (MQ=255)
                                                                               tACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  8:197785/138‑1 (MQ=255)
                                                                               tACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  6:254488/138‑1 (MQ=255)
                                                                               tACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  8:202540/138‑1 (MQ=255)
                                                                               tACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  1:301435/138‑1 (MQ=255)
                                                                               tACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  3:112869/138‑1 (MQ=255)
                                                                               tACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  7:18697/138‑1 (MQ=255)
                                                                               tACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  4:86605/138‑1 (MQ=255)
                                                                               tACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  2:255247/138‑1 (MQ=255)
                                                                                aCGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  7:335806/137‑1 (MQ=255)
                                                                                aCGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            >  8:335806/1‑137 (MQ=255)
                                                                                aCGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCa                                                           <  8:177801/138‑1 (MQ=255)
                                                                                     gtgAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCa                                                                                                                         <  4:261645/71‑1 (MQ=255)
                                                                                     gtgAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCa                                                                                                                         >  3:261645/1‑71 (MQ=255)
                                                                                      tgAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAACGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGCACCCGTACGTCCGATTGAGATGGTCAACGCCTa                                                    >  3:179629/1‑139 (MQ=255)
                                                                                      tgAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGTATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTa                                                    >  4:188695/1‑139 (MQ=255)
                                                                                      tgAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTa                                                    >  1:173151/1‑139 (MQ=255)
                                                                                      tgAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTa                                                    >  5:148195/1‑139 (MQ=255)
                                                                                            cGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGTATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTATGCCAt                                              <  3:188695/139‑1 (MQ=255)
                                                                                                tCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACg                                                                            <  6:268198/105‑1 (MQ=255)
                                                                                                tCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACg                                                                            >  5:268198/1‑105 (MQ=255)
                                                                                                tCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTgaga                                                                 >  7:285790/1‑116 (MQ=255)
                                                                                                tCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTgaga                                                                 <  8:285790/116‑1 (MQ=255)
                                                                                                tCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCAACTTCAAGCACTGCAGTCCCTGGCTCTCGGCACCGAACCCGTTCGTCCGATTGAGATGGTCAACGCCTAAGCCAGGCTc                                          >  1:127036/1‑139 (MQ=255)
                                                                                                                          tAACGACGGCAAAGGAAATCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGCGATGGTCAACGCCTATGCCATGCTCGCAAATGGCGGAAAGAAAATCGAAcc                <  2:400773/139‑1 (MQ=255)
                                                                                                                          tAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTATGCCATGCTCGCAAATGGCGGAAAGAAAATCGAAcc                >  8:88455/1‑139 (MQ=255)
                                                                                                                              gACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTATGCCATGCTCGCAAATGGCGGAAAGAAAATCGAACCGACt            <  2:133035/139‑1 (MQ=255)
                                                                                                                                 ggCAAAGGTATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            <  3:209202/88‑1 (MQ=255)
                                                                                                                                 ggCAAAGGTATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTc                                                            >  4:209202/1‑88 (MQ=255)
                                                                                                                                 ggCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTATGCCATGCTCGCAAATGGCGGAAAGAAAATCGAACCGACttt          >  6:314600/1‑138 (MQ=255)
                                                                                                                                      agtaaTTCTGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCTCTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTATGCCATGCTCGCAAATGGCGGAAAGAAAATCGAACCGACTTTTATTTc    <  8:274914/136‑1 (MQ=255)
                                                                                                                                        gAATTCGGCATTACCGCCCATCTTCACGCACTGCCGGCCCTGGCACTCGGCACCGAACCCGTACGTCCGTTTGAGATGGTCAACGCCTATGCCATGCTCGCAAATGGCGGAAAGAAAATCGAACCGACTTTTATTTCaa  <  1:394169/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TTCTCCAAGCAACTATAACGGATACTATGCCAACAAGCCGATTACGCTTCTGCAGGCGCTGGCGCTTTCCGACAATATTTACGCTGTGAAAACGCATCTTTTTCTCGGCACGAACAAACTTGTAAAGACGGCAAAGGAATTCGGCATTACCGCCCATCTTCAAGCACTGCCGTCCCTGGCACTCGGCACCGAACCCGTACGTCCGATTGAGATGGTCAACGCCTATGCCATGCTCGCAAATGGCGGAAAGAAAATCGAACCGACTTTTATTTCAA  >  CP006881/3668878‑3669152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑