Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 263,409 A→C 20.1% A255A (GCT→GCG)  U712_01235 ← putative sodium/glutamine symporter glnT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881263,4090AC20.1% 80.4 / 8.5 48A255A (GCT→GCG) U712_01235putative sodium/glutamine symporter glnT
Reads supporting (aligned to +/- strand):  ref base A (18/20);  new base C (6/4);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTGGATCAGCCCCTGTTTGACGGGATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCGATATACAGGACAGCCAATACGACGACAATG  >  CP006881/263281‑263544
                                                                                                                                |                                                                                                                                       
gCTTGGATCAGCCCCTGTTTGACGGGATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCcgg                                                                                                                               >  1:322967/1‑139 (MQ=255)
   tGGATCAGCCCCTGATTGACGGGATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGCCGCCTTGCATGAGTGCCGCGCCCAACGCCCCGCcggcgg                                                                                                                            >  3:174382/1‑139 (MQ=255)
     gATCAGCCCCTGTTTGACGGGATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCggcggc                                                                                                                           <  7:289627/138‑1 (MQ=255)
           ccccTGTTTGACGGGATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCCCCGGCGGCCTGGTc                                                                                                                     >  3:187294/1‑138 (MQ=255)
                gTTTGACGGGATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCGTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAAcc                                                                                                               >  8:391041/1‑139 (MQ=255)
                     aCGGGATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAACACCAAACg                                                                                                          >  6:401015/1‑139 (MQ=255)
                       gggATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCa                                                                                                        <  4:187294/139‑1 (MQ=255)
                          aTGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAACATTCCCCGTCTCACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCCCCGGCGGCCTGGTCAAAACGAAACCCAttt                                                                                                     >  3:92016/1‑139 (MQ=255)
                           tGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAACGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCAtttt                                                                                                    >  5:7445/1‑139 (MQ=255)
                            ggCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCAttttt                                                                                                   <  2:282771/139‑1 (MQ=255)
                                   tgtgGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGAt                                                                                             >  2:137918/1‑138 (MQ=255)
                                   tgtgGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGACGCGCCCAACGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGAt                                                                                             >  7:231492/1‑138 (MQ=255)
                                     tgGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAg                                                                                          <  2:186199/139‑1 (MQ=255)
                                       gCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCggcggc                                                                                                                           <  5:385735/104‑1 (MQ=255)
                                       gCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCggcggc                                                                                                                           >  6:385735/1‑104 (MQ=255)
                                       gCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACCCCTTGCATGAGTGCAGCGCCCAACGCCCCGCCGGCGGCCTGGACAACACCAACCAGATTATTCACCAtacgcc                                                                                        >  7:362168/1‑134 (MQ=255)
                                        cGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAACGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAgcg                                                                                        >  7:185377/1‑138 (MQ=255)
                                         ggTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCa                                                                                      <  2:322967/139‑1 (MQ=255)
                                           tGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAg                                                                                    <  4:369591/139‑1 (MQ=255)
                                             gCAGCGGCATTTGGTGCGCTTCCCATTCCCTCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGca                                                                                  <  5:277469/139‑1 (MQ=255)
                                             gCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGca                                                                                  <  1:216293/139‑1 (MQ=255)
                                             gCAGCGGCATTTGGTGCGCGTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGAGGCCTGGGCAAAACCAAACGCATTTTTCACGATTAGCGCAAGca                                                                                  <  7:223892/139‑1 (MQ=255)
                                                   gCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCATGCATGAGTGCCGCGCCCACAGACCCTCCGGCGGCCGGGTCAAAACCAAACCCATTTTTCACGATTAGAGCAAGCACACCTg                                                                            >  4:4811/1‑139 (MQ=255)
                                                        tGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAg                                                                        <  7:40926/138‑1 (MQ=255)
                                                        tGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGAAGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGGCTGGTCAAAACGAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGGAg                                                                        <  6:220/138‑1 (MQ=255)
                                                             cgcTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCg                                                                   <  8:340149/138‑1 (MQ=255)
                                                                         ccGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGgaga                                                       >  7:339345/1‑138 (MQ=255)
                                                                            cTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAAt                                                   >  5:309371/1‑139 (MQ=255)
                                                                            cTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAAt                                                   >  1:16718/1‑139 (MQ=255)
                                                                            cTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAAt                                                   >  1:255721/1‑139 (MQ=255)
                                                                                   tGAAATGATTCCCCGTCTGACGCCTTGCTTTAGTGCCGCGCCCAAAGCCCCGCCGGGGGCATGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTATTTTTGGAGAAAATGACGaa                                             <  3:324377/138‑1 (MQ=255)
                                                                                          aTTCCCCGTCTGCCGCGTTGCATGAGTGCCGCGCCCAACGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCTGCTGCTTAATGTTGGAGAAAATGACGAAAAACGCaa                                     <  1:131645/139‑1 (MQ=255)
                                                                                          aTTCCCCGTCTGACGCCTTGGAGGCGGGCCGCGCACAACGCCCCGCGGGCGGCCGGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAACCACACCTGGCAGCTGCGTACTGTTGGAGAAAATGACTAAAAACGCaa                                     <  8:205597/139‑1 (MQ=255)
                                                                                          aTTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAGACGCaa                                     >  2:164000/1‑139 (MQ=255)
                                                                                           ttCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAAc                                    >  2:345636/1‑139 (MQ=255)
                                                                                           ttCCCCGGCTGTCCCCTTGCATGAGTCCCGCGCCAAAAGCCCCGCGGGCGGCCTGGTCAAAACCAAACGCTTTTTTCACGATTAGCGCAAGCACACCTGGCCGCTGCGTAATGTTGTATAAAATCACCAAAAACGCaa                                     <  8:185377/138‑1 (MQ=255)
                                                                                            tCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAAc                                    <  7:60797/138‑1 (MQ=255)
                                                                                               ccGTCTGACGCCTTGCATGTGGGCCGCGCCAAAAGCCCCGCCGGGGGCCTGGTAAAAACAAAACGAATTTTTCACTATTCGGGCAAGCACACTTGGCTGGTGCTTAATTTTGTAAAAAATTCCTAAAACCGCAACTCCg                                <  8:231492/139‑1 (MQ=255)
                                                                                               ccGTCTGACGCCTTGCATGCGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAACCGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGACAAAATGACCAAAAACGCAACTCCg                                <  7:320670/139‑1 (MQ=255)
                                                                                               ccGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCg                                <  7:391041/139‑1 (MQ=255)
                                                                                               ccGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCGGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCg                                <  5:170404/139‑1 (MQ=255)
                                                                                               ccGTCTGACGCCTGGCATGAGGGCCGCGCCCAACGCCCCGCCGGCGGCCGGGTCAAACCCAACCGCATTTTTCACTATTAGCGCACGCACCCGTGGCAGCGGCGTAATGTGGGATAAAATGACGAAAAACGCAACTCCg                                <  2:255721/139‑1 (MQ=255)
                                                                                                         ccTTGCATGAGGGCCGCGCCCAACCCCCCGCGGGCGGCGTGGACAAAACCAAACGATTTTTTCACGATTCCCCAAACCACACCTGGCAGGGGCTTAATGTTGGACAAAATCACCAAACACGCAACTCCGATATACAGGa                      <  6:7445/139‑1 (MQ=255)
                                                                                                            tGCATGAGTGCCGCGCCCAACGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCCCGATTAGCGCAAGCCCACCTGGCAGCGGCTTAATGTTGGAGAATATGACGAAAACCGCACCTCCGAGATCCAGGACAg                   >  1:358649/1‑139 (MQ=255)
                                                                                                            tGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCGATATACAGGACAg                   >  1:141448/1‑139 (MQ=255)
                                                                                                                    tGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCGATATACAGGACAGCCAATacg           >  1:367151/1‑139 (MQ=255)
                                                                                                                    tGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCGATATACAGGACAGCCAATac            >  5:4370/1‑138 (MQ=255)
                                                                                                                         cgcCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCGATATACAGGACAGCCAATAcgacga       >  7:19058/1‑138 (MQ=255)
                                                                                                                              aaaGCCCCGCCGGCGGCCTGGTCAAACCCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCGATATACAGGACAGCCAATACGACGACAATg  >  8:22298/1‑138 (MQ=255)
                                                                                                                              aaaGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCGATATACAGGACAGCCAATACGACGACAATg  >  1:28841/1‑138 (MQ=255)
                                                                                                                                |                                                                                                                                       
GCTTGGATCAGCCCCTGTTTGACGGGATGGCTTGTTGTGGCGGTGGCAGCGGCATTTGGTGCGCTTCCCATTCCCGCTTCGTTTGAAAAGATTCCCCGTCTGACGCCTTGCATGAGTGCCGCGCCCAAAGCCCCGCCGGCGGCCTGGTCAAAACCAAACGCATTTTTCACGATTAGCGCAAGCACACCTGGCAGCTGCGTAATGTTGGAGAAAATGACGAAAAACGCAACTCCGATATACAGGACAGCCAATACGACGACAATG  >  CP006881/263281‑263544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑