| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,725,239 | 2 bp→AA | 100% | intergenic (‑71/‑457) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,239 | 0 | G | A | 95.7% | 82.0 / ‑5.9 | 29 | intergenic (‑71/‑458) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/5); minor base C (2/0); total (24/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | CP006881 | 3,725,240 | 0 | G | A | 100.0% | 82.2 / NA | 29 | intergenic (‑72/‑457) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/5); total (24/5) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑T‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAG > CP006881/3725119‑3725363 || ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACTGCCGAtt > 8:170490/1‑139 (MQ=255)ttCATAAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGATTCATAGAAAAACATCCCACCCCGTCTATTTGGCAGGCAGC‑C‑TGTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGcccagt > 8:400240/1‑135 (MQ=255) tcatcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCTGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAttt > 7:160464/1‑139 (MQ=255) aTCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCACC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCAAGCaaa > 7:380341/1‑138 (MQ=255) aTCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCACGCAGC‑CAT‑TTTAGCACCCCGGTTTCTCAGCCGCAGCCCCCCAGAAAAGCCCGCCGCATTCTAATCTCGCaaa > 6:242390/1‑138 (MQ=255) aaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGAAGCCCGTTTTCTCAGCCGCAGCCCGCAACAAAAGACGGCCGATTTGTTTTCACGCAAACTAGCGGCTCTAg > 3:175736/1‑138 (MQ=255) aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCACCCCGTTTTCTCAGCCGCCCCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 3:337730/139‑1 (MQ=255) aTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCCGCCCGTTTTCTCAGCCGCCGCCCGCCAGAAAAGACGGCCGCTTTCTTTTCTCGCAACCTAGCGGCGCTATCaa > 8:357591/1‑138 (MQ=255) aTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaa > 4:361695/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa > 8:12548/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa > 1:277184/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 3:288943/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 8:342772/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTAGTTTTGGCAGGCAGC‑CAT‑TTTAGCAGCCCGGTTTATCAGCCGCAGCCCCCAAGAAAAGACGGCCGATGTATTTTATCGCAAAATAGCGCCACTAGCaaaa > 4:119252/1‑138 (MQ=255) accAACCGTTTCATATAAAAACCTCCCTCCGTTTCTTTTTGGCAGCCCGC‑CTT‑TTTAGCCGCCCGTTTCCGCCGCCCCCCCCCTAAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa < 8:29728/137‑1 (MQ=255) ccAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAGCTT‑T‑TTTAGCAGCCCGTTTTCTCCCCCGCAGCCCGCAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 8:14399/138‑1 (MQ=255) aaaCGTTTCATACAAAAACTCCCCTCCGCTTCTTTTTGGCAGGCAGCTT‑T‑TTTAGCAGCCCGTTTTCTCAGCGGCAGCCCGAAAGAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 3:361695/139‑1 (MQ=255) cATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTGATTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGGCACTaag > 3:275637/1‑136 (MQ=255) cATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGa > 2:14886/1‑139 (MQ=255) cGCTTCTTTTTGGCAGGCAGC‑CTT‑TTTAGCAGCCCGTTTTATCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCCAACTACCGGCTCGAGCAAAACAACAGACACTATGATAGCCCCGGGAAAGAGaa > 6:400038/1‑139 (MQ=255) ggcagC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCAAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCATTGTCACAata > 1:285662/1‑139 (MQ=255) gcagC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATTACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAatac > 7:323104/1‑138 (MQ=255) gcagC‑CTT‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGAGGCTCTAGCAACACTCATGACTCGATTCTAGCCCGTGGAAAGCGAATTCTTTGTCACAatat > 6:102206/1‑139 (MQ=255) t‑tttAGCAGCCCGTTTTCTCAGCCGCAGCCCGAAAGAAAATAAGTCCGAATTATTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGTAAAGAGAATTATTTGTAAAAAAATGATTGa > 2:62351/1‑138 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATATACCGTGGAAAGAGAATTCTTTGTCACAATAAGAGTGAAAAAAACaa > 6:165106/1‑138 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCaa > 3:375442/1‑138 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc > 3:362589/1‑139 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATCGCAAACTAGCGGCTCTAGCAAAACTCATGACTGTATGTTAGACCGTCGAATGAGAATTGTTTGTCACAATATGAGTTAAAAAACCac > 6:294307/1‑137 (MQ=255) cAGCCGCTGCCCGCAAGAAAGGACGTCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAg < 8:42063/139‑1 (MQ=255) || TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑T‑TTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAG > CP006881/3725119‑3725363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |