Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,725,031 C→T A46A (GCG→GCA)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,0310CT98.1% 175.4 / NA 53A46A (GCG→GCA) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/45);  minor base A (1/0);  total (8/45)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT  >  CP006881/3724896‑3725165
                                                                                                                                       |                                                                                                                                      
cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCTTTGTGCTTTTCTTCGTCATTGAAAGCCCTCCGCCATTCATAAAAAGAAACGAGTCCagcg                                                                                                                                     >  3:166755/1‑139 (MQ=255)
cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                                                                                     <  3:252159/139‑1 (MQ=255)
  ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc                                                                                                                                   >  8:330761/1‑139 (MQ=255)
       gCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCTTTGGTCTTTTAATACTAATTAACAACCAACCCCCAATCATAAAAAGAAACGACTCCAGCCGCCAGcc                                                                                                                              >  6:169693/1‑138 (MQ=255)
             tcCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagc                                                                                                                          <  3:264758/137‑1 (MQ=255)
               cTGCTTTGGCGATTCCTTGACGGCGTCCAAACATCTTCTTTTAAACCGCGCGCCGGCTTGCGCTTTCTGCTTTTCTTCCTCATTCACCCCCACCCGCCATCAAAAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                      <  2:231234/139‑2 (MQ=255)
               cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagccg                                                                                                                        <  5:394930/137‑1 (MQ=255)
               cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                      <  8:452827/139‑2 (MQ=255)
               cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                      <  2:172306/139‑2 (MQ=255)
                      ggCGATTCCTTGAAGGCTTGCAAACATATTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                               <  1:205388/139‑1 (MQ=255)
                       gCGATTCCTTGAAGGCTTGCAAACATGTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATACGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                               <  5:384655/138‑1 (MQ=255)
                       gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                               <  3:113534/138‑1 (MQ=255)
                       gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                               <  6:274421/138‑1 (MQ=255)
                       gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                               <  7:330761/138‑1 (MQ=255)
                            tCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATg                                                                                                         >  8:176358/1‑139 (MQ=255)
                                 gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                     <  1:326544/138‑1 (MQ=255)
                                    ggCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGa                                                                                                 <  7:390352/139‑1 (MQ=255)
                                    ggCTTGCAAACATCTTC‑TTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  2:73807/139‑1 (MQ=255)
                                    ggCTGGCAAACATCTTC‑TTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  2:73812/139‑1 (MQ=255)
                                     gCTTGCAAACTTCTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCAATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  2:459058/139‑1 (MQ=255)
                                     gCTTGCAAACATCTTCTTTTATTAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAATCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  5:196249/139‑1 (MQ=255)
                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAACCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  5:348562/139‑1 (MQ=255)
                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  4:280667/139‑1 (MQ=255)
                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  5:169693/139‑1 (MQ=255)
                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  3:360280/139‑1 (MQ=255)
                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  6:464603/139‑1 (MQ=255)
                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  3:121587/139‑1 (MQ=255)
                                     gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                <  7:285665/139‑1 (MQ=255)
                                                tcttctTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTTTTTTCGCGTGGCCGAAAAGAcc                                                                                     >  2:11347/1‑138 (MQ=255)
                                                                       cTTTCGCTTTCTGCTTTTCTTCCTCATTCACCGCCCTCCCCCATTCAAAACACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                              <  5:275495/139‑1 (MQ=255)
                                                                       cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                              <  8:482583/139‑1 (MQ=255)
                                                                       cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                              <  1:455646/139‑1 (MQ=255)
                                                                                    cttttCTTCCTCATACACCGCCATCCGCCATTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                                    <  3:263022/135‑1 (MQ=255)
                                                                                       acagtcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                              <  3:99150/135‑1 (MQ=255)
                                                                                        tcttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                              <  1:206359/138‑1 (MQ=255)
                                                                                        tcttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                              <  1:308136/138‑1 (MQ=255)
                                                                                          ttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                               >  8:329861/1‑71 (MQ=255)
                                                                                          ttcCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                               <  7:329861/71‑1 (MQ=255)
                                                                                            ccTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTTTTTACCCTGAGCCCCATAGACCCCGGCCCCCCCCGGTTTTGTGTACGGCGGCACCCCGTtcagac                                          >  3:272213/1‑135 (MQ=255)
                                                                                             cTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGTGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCaa                                         <  3:270838/138‑1 (MQ=255)
                                                                                             cTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCaaa                                        <  7:200931/139‑1 (MQ=255)
                                                                                                 ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                    <  1:54711/139‑1 (MQ=255)
                                                                                                 ttCACAGACATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                    <  3:174788/139‑1 (MQ=255)
                                                                                                      ggCCATCCGCCATTCATAAAACGAAACGCGTCCTGCGGCCTGCACAGCCGCCTTGCTTTGGTATGTACGCTGAGCCGATTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCTCCCAGTTCATCAAAAAGACCATc                               <  5:309434/138‑1 (MQ=255)
                                                                                                      aGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATc                               <  4:166755/139‑1 (MQ=255)
                                                                                                        ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                             <  7:168579/139‑1 (MQ=255)
                                                                                                        ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                             <  8:147895/139‑1 (MQ=255)
                                                                                                         cATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcac                            <  7:66779/139‑1 (MQ=255)
                                                                                                         cATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcac                            <  3:478085/139‑1 (MQ=255)
                                                                                                          aTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca                           <  2:108103/139‑1 (MQ=255)
                                                                                                                cattCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTGTGTCCGGCGTCCCCCAGTTCATCCAAAAGACCCTCCCCCCCCgg                      >  3:108462/4‑137 (MQ=255)
                                                                                                                      tAAACCGAAACGAGTCCTGCGGCCTGCACAGGCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa               <  3:426914/139‑1 (MQ=255)
                                                                                                                      tAAAACGAAACGAGTCCTGCGGCCTGCACAGCAGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa               <  3:434180/139‑1 (MQ=255)
                                                                                                                               acgaGTCCTGCGGCCTGCACAGCCGCCTTTGTTTGGTATGTACGCTGCGCCGAATAGCCCGCGGCCGCCGCAGGTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCCTCCCCACCCGTAAAGATAACAGCCAAc      >  8:145964/4‑139 (MQ=255)
                                                                                                                                   gTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGttt  <  1:111950/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT  >  CP006881/3724896‑3725165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑