BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,049	C→T	intergenic (+318/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,049	0	C	T	99.6%	8935.8 / NA	2524	intergenic (+318/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (845/1669); minor base G (1/8); total (847/1677)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2593 total aligned reads displayed.

TTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAC  >  pSIJ663_pHT315_araE_xy/4914‑5185

 tttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCtt                                                                                                                                       <  7:92179/138‑1 (MQ=255)

  ttatGCGCGTACACTGCTGATGCCAAAAAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                      <  2:123235/135‑1 (MQ=255)

  ttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                      <  8:353127/138‑1 (MQ=255)

  ttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                      <  5:384300/138‑1 (MQ=255)

      gcgcGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTTTTTGCCGGATCAATAGCTACCCACTCTTTTTCCGAATGTAACTGGCTTCAGCAGGGAGCAGATACCAAAAACTTTTCTTCAAgct                                                                                                                                 >  3:196686/1‑137 (MQ=255)

      gcgcGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                 >  2:453615/1‑139 (MQ=255)

      gcgcGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAAGTAACTGGCTTCAGCAGATCGCAGATACCAAATACTGATCTTCAAgtg                                                                                                                                 >  4:378927/1‑139 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtgt                                                                                                                                >  2:82436/1‑139 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                 <  7:58379/138‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                 <  5:247916/138‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                 <  2:154812/138‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAACTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                 <  7:367876/138‑1 (MQ=255)

         cgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGGTTGTTTGCCGGCTCAAGAGCTACCAACTCTTTTTCCGAAGGTAAAAGGCTTCAGCAGCGCGCAGAGACCAAAAACAGTTCTTCTAGTGTat                                                                                                                              >  6:266341/1‑138 (MQ=255)

           tAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                            <  6:223611/139‑1 (MQ=255)

                 gctgctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCACCTCTTTTTCCGAAGGTAAATTGCTTCAGCAGAGCGCAGAAACCAAATACAGTTCTTCTAGTGTAGCCgattt                                                                                                                       >  8:31175/1‑134 (MQ=255)

                 gctgctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGtt                                                                                                                      >  6:472040/1‑139 (MQ=255)

                    gctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                            <  4:7265/130‑1 (MQ=255)

                    gctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTTgg                                                                                                                   >  1:95900/1‑139 (MQ=255)

                       tGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCACCTCTTTTTACGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACAGTTCATCTAGTGTAGCCGGAGTTAGGcca                                                                                                                >  7:276221/1‑139 (MQ=255)

                       tGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATAAAGAGCTACCAACTCTTTTTCCCAAGGTAACATGCTTCAGCAGCGCGCAGAGACCAAATAATGTTGTTCTAGTGTAGCCGTAGTTAGTcca                                                                                                                >  2:278906/1‑139 (MQ=255)

                        gCAAACAAAACAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCATAGTTAGGcc                                                                                                                 <  4:151692/137‑1 (MQ=255)

                         caaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                               <  5:415156/138‑1 (MQ=255)

                         caaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGAGGAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGATCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                              <  3:374276/139‑1 (MQ=255)

                                 aaaaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                      <  5:348918/139‑1 (MQ=255)

                                   aaCCACCGCTTCGAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  6:388451/139‑1 (MQ=255)

                                   aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  6:16156/139‑1 (MQ=255)

                                   aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  7:157746/139‑1 (MQ=255)

                                   aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  7:187045/139‑1 (MQ=255)

                                   aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  7:333150/139‑1 (MQ=255)

                                   aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  4:355649/139‑1 (MQ=255)

                                   aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  8:72316/139‑1 (MQ=255)

                                   aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  5:121424/139‑1 (MQ=255)

                                   aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  2:35099/139‑1 (MQ=255)

                                   aaCCACCGCGCCCAGCGGTGGTTTGTTTGCCGGATCAAGAGCAACCAACTCATTATCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGGTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  5:78702/139‑1 (MQ=255)

                                        ccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTg                                                                                               >  1:137655/1‑139 (MQ=255)

                                           cTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                            <  4:459696/139‑1 (MQ=255)

                                             aCCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTATTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCa                                                                                          >  8:12357/1‑139 (MQ=255)

                                              ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGCAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                         <  3:341467/139‑1 (MQ=255)

                                              ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGc                                                                                           <  2:234525/137‑1 (MQ=255)

                                              ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCa                                                                                          <  3:75447/138‑1 (MQ=255)

                                              ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                         <  5:270602/139‑1 (MQ=255)

                                              ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                         <  4:307286/139‑1 (MQ=255)

                                              ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                         <  3:442656/139‑1 (MQ=255)

                                              acatcGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                         <  7:218836/135‑1 (MQ=255)

                                               cAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                        <  4:191668/139‑1 (MQ=255)

                                               cAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                        <  8:482917/139‑1 (MQ=255)

                                                aGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  4:413509/126‑1 (MQ=255)

                                                    gtggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                 >  4:267454/1‑93 (MQ=255)

                                                     tggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAc                                                                                  <  3:268928/139‑1 (MQ=255)

                                                     tggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAc                                                                                  <  1:229827/139‑1 (MQ=255)

                                                     tgggTTGTTTGCCGGATCAAGAGCTACCAACGGGTTTTCCGCAGGTAAATGGCTTCAGCAGAGCGCAGCTACCCAAAACTGTTCTTGTTGTGTTGCCGGAGTTTTGCCCCCCCCTCAAGCACTCAGTAGCCCCGccgg                                                                                   >  6:304675/1‑136 (MQ=255)

                                                        tttgtttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATa                                                                               <  2:192078/139‑1 (MQ=255)

                                                            tttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGa                                                                                                     >  6:345837/1‑113 (MQ=255)

                                                              tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                          <  3:23811/138‑1 (MQ=255)

                                                               gCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                         <  5:181684/138‑1 (MQ=255)

                                                               gCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                         <  3:127716/138‑1 (MQ=255)

                                                                ccGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctc                                                                       <  2:409482/139‑1 (MQ=255)

                                                                ccGGATCAAGAGCTACCAACTCTTTTTCCGAACGTAACTGGCTTCAGCAGAGCGCAGATACCTAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctc                                                                       <  5:150096/139‑1 (MQ=255)

                                                                 cGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctc                                                                       <  8:232759/138‑1 (MQ=255)

                                                                  ggATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTg                                                                     >  3:505870/1‑139 (MQ=255)

                                                                     tCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTa                                                                  <  4:230346/139‑1 (MQ=255)

                                                                             tACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTCtt                                                          >  1:493196/1‑139 (MQ=255)

                                                                              aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                      <  2:322884/62‑1 (MQ=255)

                                                                              aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTa                                                         >  5:457475/1‑139 (MQ=255)

                                                                              aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAAAACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCCCTCTGCTAATCCTGTTa                                                         >  3:15620/1‑139 (MQ=255)

                                                                               ccAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAc                                                        >  4:500347/1‑139 (MQ=255)

                                                                                  aCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAg                                                     <  4:107186/139‑1 (MQ=255)

                                                                                    tctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                 <  5:215111/61‑1 (MQ=255)

                                                                                      tttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                      >  3:472388/1‑86 (MQ=255)

                                                                                       ttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGt                                                    >  1:102463/1‑135 (MQ=255)

                                                                                        tttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgct                                                <  1:416887/138‑1 (MQ=255)

                                                                                         ttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGt                                                                                                                       >  7:12317/1‑66 (MQ=255)

                                                                                         ttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                         >  3:417246/1‑112 (MQ=255)

                                                                                          tCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgctgc                                              <  1:29142/138‑1 (MQ=255)

                                                                                               aGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAg                                        <  7:246681/139‑1 (MQ=255)

                                                                                                ggTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGt                                       <  6:42816/139‑1 (MQ=255)

                                                                                                     cTGTCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGACGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGACTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGa                                  <  8:203934/139‑1 (MQ=255)

                                                                                                           tCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                         >  8:437147/1‑78 (MQ=255)

                                                                                                           tCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                         <  7:437147/78‑1 (MQ=255)

                                                                                                            cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                              >  5:325209/1‑56 (MQ=255)

                                                                                                            cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                        >  6:78040/1‑78 (MQ=255)

                                                                                                            cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGt                             <  3:302191/137‑1 (MQ=255)

                                                                                                                  agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                    <  8:400181/60‑1 (MQ=255)

                                                                                                                  agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                          <  6:135320/86‑1 (MQ=255)

                                                                                                                  agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCtt                     >  1:198216/1‑139 (MQ=255)

                                                                                                                  agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCt                      >  1:72841/1‑138 (MQ=255)

                                                                                                                   gcgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTa                    <  2:500891/139‑1 (MQ=255)

                                                                                                                    cgcAGATACCAAATACTGTTCTTCTAGTGTATCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                   >  6:503442/1‑139 (MQ=255)

                                                                                                                    cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                         <  8:27298/85‑1 (MQ=255)

                                                                                                                    cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTa                                                                  <  1:460635/92‑1 (MQ=255)

                                                                                                                    cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgtg                         >  7:476416/1‑133 (MQ=255)

                                                                                                                    cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgtg                         >  1:339815/1‑133 (MQ=255)

                                                                                                                       aGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgtg                         >  8:156516/1‑130 (MQ=255)

                                                                                                                       aGATACCAAATACTGTTCTACTAGTGTAGCCGTAGTAAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGATCTGCTAATCCTGTTACCAGTGGCTGCTTCCAGTGGCGCTAAgtc‑tgtctctaata                >  1:379681/1‑132 (MQ=255)

                                                                                                                          tACCAAATACTGTGCTTCTAGTGTAGCCGTAGTAAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGtt             <  6:105127/139‑1 (MQ=255)

                                                                                                                              aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGc                                                                                      >  1:164964/1‑62 (MQ=255)

                                                                                                                              aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGa          >  1:264834/1‑138 (MQ=255)

                                                                                                                              aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGAc         >  5:50027/1‑139 (MQ=255)

                                                                                                                               aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACAGGGTTGGACt        <  6:187824/139‑1 (MQ=255)

                                                                                                                                    tGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACt        <  7:406479/134‑1 (MQ=255)

TTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAC  >  pSIJ663_pHT315_araE_xy/4914‑5185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`