BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,205	G→A	intergenic (+474/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,205	0	G	A	97.5%	10341.8 / NA	3415	intergenic (+474/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base G (27/19); major base A (1580/1692); minor base C (67/17); total (1680/1735)
Fisher's exact test for biased strand distribution p-value = 6.25e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3419 total aligned reads displayed.

AGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTAT  >  pSIJ663_pHT315_araE_xy/5070‑5340

 ggCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGTTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAg                                                                                                                                      <  4:208503/138‑1 (MQ=255)

   ccaccaCTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCgg                                                                                                                                   <  8:330943/139‑1 (MQ=255)

        acTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCGCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCggg                                                                                                                              <  1:140373/139‑1 (MQ=255)

            cAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCt                                                                                                                            <  3:270165/137‑1 (MQ=255)

            cAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGa                                                                                                                          >  4:175350/1‑139 (MQ=255)

                    ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTGTTACCGGGTTGGAGTCGAGACGATTGTTAACGGTTGAGGGAGCGGGGGCGGGCGGGACgggggg                                                                                                                  >  2:96947/1‑139 (MQ=255)

                    ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTGTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                                                  >  1:386106/1‑139 (MQ=255)

                    ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                                                  >  3:317138/1‑139 (MQ=255)

                    ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                                                  >  6:91294/1‑139 (MQ=255)

                    ctGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                                                  >  1:317277/1‑139 (MQ=255)

                            ccGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGTCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGaa                                                                                                                         <  2:222157/124‑1 (MQ=255)

                             cGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGc                                                                                                                             >  6:506921/1‑119 (MQ=255)

                             cGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGCACGGGGGGTTCGTGcac                                                                                                         >  6:246528/1‑139 (MQ=255)

                              gCCTACATACCTCGGTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        <  5:327863/139‑1 (MQ=255)

                              gCCTACATACCTCGCTCTGCTAATCCTGTTAGCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACTGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        <  1:252451/139‑1 (MQ=255)

                              acagaCATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCTGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        <  1:171445/135‑1 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTGTTACCGGGTTGGCCGCAAGCCGATAGTTACCGGATTAGGCACAGCGGGCCGGCTGCACCGGGGGTTCGTGcgcac                                                                                                       >  7:187014/1‑139 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                                                       >  2:16947/1‑139 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                                                       >  8:236586/1‑139 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        >  1:344591/1‑138 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGCGcccac                                                                                                       >  4:332979/1‑139 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGGTCGTGcaccc                                                                                                       >  3:448684/1‑137 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCGGAACGGGGGGTTCGTGcaca                                                                                                        >  5:446327/1‑138 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAACAAGATATTTTCAGGATAAGTCACAGCGCTCGGGCTGGACCGGGGGTTCGTGaaca                                                                                                        >  4:284585/1‑138 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAACGGGTTGGACTCACGCCGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGCACGGGGGGTTCGTGccca                                                                                                        >  7:280596/1‑138 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCGTACCGGGTTGGACTCAAGCCGATAGGTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                                                       >  3:6347/1‑139 (MQ=255)

                               ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTCGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGGTGAACGGGGGGTTGGTGcacac                                                                                                       >  4:413221/1‑139 (MQ=255)

                               ccTACATACCTCGCTCGGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                                                       >  1:28028/1‑139 (MQ=255)

                                   cATTCCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTTGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGcc                                                                                                   <  7:219552/139‑1 (MQ=255)

                                   cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGc                                                                                                    <  4:245435/138‑1 (MQ=255)

                                   cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGc                                                                                                    <  8:158467/138‑1 (MQ=255)

                                    aTACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCGGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGccc                                                                                                  <  5:467772/139‑1 (MQ=255)

                                       ccTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGc                                                                                               <  2:389257/139‑1 (MQ=255)

                                        cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        <  5:125959/129‑1 (MQ=255)

                                          cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        <  7:96430/127‑1 (MQ=255)

                                          cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                            >  2:437198/1‑139 (MQ=255)

                                          cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                            >  5:403875/1‑139 (MQ=255)

                                          cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                            >  5:506468/1‑139 (MQ=255)

                                          cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                            >  7:31723/1‑139 (MQ=255)

                                          cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGTCTCCAGACCATAGTTACCGGTTTAGGCTCCGCGGTCGGGATGAAGCGGGGGTTCGTGCCCACACCCCatcggg                                                                                            >  5:370327/1‑134 (MQ=255)

                                             tctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAg                                                                                         >  5:191789/1‑139 (MQ=255)

                                             tctGCTAATCCTGTTACCAGTGGCTACTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCCCAGCGGTCGGGCTGCCCGGGGGGTTCGTGCCCCCAGCCCAGCTTggcg                                                                                         >  8:123118/1‑137 (MQ=255)

                                                 cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                      <  5:292165/138‑1 (MQ=255)

                                                 cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACTGCGGGCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                      <  8:204842/138‑1 (MQ=255)

                                                 cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                      <  3:23635/138‑1 (MQ=255)

                                                 cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                      <  5:103488/138‑1 (MQ=255)

                                                 cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACCATAGTTACCGGAGAAGGAACACCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCATCTTGGAGCGa                                                                                      >  8:296615/1‑138 (MQ=255)

                                                    aTCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTGCGTGCACACAGCCCAGCTTGGAGCGAACg                                                                                   <  5:222271/138‑1 (MQ=255)

                                                     tCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAc                                                                                 <  7:65738/139‑1 (MQ=255)

                                                         gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCgg                                                                                                                                   >  6:278828/1‑85 (MQ=255)

                                                         gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACCGCCCAGCTTGGAGCGAACGACCTac                                                                             >  4:456933/1‑139 (MQ=255)

                                                         gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGGCCTac                                                                             >  1:942/1‑139 (MQ=255)

                                                         gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAc                                                                                 >  8:482970/1‑135 (MQ=255)

                                                         gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTac                                                                             >  3:93804/1‑139 (MQ=255)

                                                         gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCGTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACCGCGGGCGGGGTGAACGGGGGGGTCGTGCACCCCGCCCCGCGTGGCGCGAACGCCCGac                                                                             >  6:44801/1‑139 (MQ=255)

                                                          ttACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGTGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTaca                                                                            <  1:48202/139‑1 (MQ=255)

                                                           tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGc                                                                                                                             <  5:252941/89‑1 (MQ=255)

                                                           tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAc                                                                                 >  8:445576/1‑133 (MQ=255)

                                                           tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTacac                                                                           >  8:66796/1‑139 (MQ=255)

                                                           tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTacac                                                                           >  2:65443/1‑139 (MQ=255)

                                                             ccAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGGTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCg                                                                         <  2:337086/139‑1 (MQ=255)

                                                              cAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCATACACGGTTGGGCACAAGACGATATTTAACCGATAAGGCACACCGGTCGGGCTGAACGGGGGGTTAGTGCACACAGCCCAGCTGTGAGCGAACGACCGACACCg                                                                         >  4:66223/1‑138 (MQ=255)

                                                               aGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa                                                                       <  7:435307/139‑1 (MQ=255)

                                                                gTGGCTGCAGCCAGTGGCGATAAGTCGTGGCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATCAGGCACAGCGGTCGGGCTGAAC‑GGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt                                                                     <  1:439592/139‑1 (MQ=255)

                                                                 tGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt                                                                     <  7:474057/139‑1 (MQ=255)

                                                                   gctgctGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga                                                                   <  3:169868/139‑1 (MQ=255)

                                                                   gctgctGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga                                                                   <  4:139693/139‑1 (MQ=255)

                                                                    ctgctgCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        <  6:323027/101‑1 (MQ=255)

                                                                    ctgctgCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt                                                                     >  6:365430/1‑136 (MQ=255)

                                                                    ctgctgCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt                                                                     >  1:20364/1‑136 (MQ=255)

                                                                      gctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGAt                                                                <  3:193059/139‑1 (MQ=255)

                                                                                gCGATTAGTCGTGTCATCCCGGGTTGTTCTCAAGACGATAGTTACCTGATTAGGAACAGCGGTGGGGCTGAACGGGGGGTTCGTGCAGACTGCCCAGCTTGGAGCGTACGACCTACACCGAACTGAGATACCTACAGCg                                                      <  3:492700/139‑1 (MQ=255)

                                                                                gCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCg                                                      <  6:180589/139‑1 (MQ=255)

                                                                                  gATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg                                                    <  3:346534/139‑1 (MQ=255)

                                                                                   aTAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt                                                                     >  4:373842/1‑121 (MQ=255)

                                                                                    tAAGTCGGGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAg                                                  <  2:306358/139‑1 (MQ=255)

                                                                                        tCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTa                                               <  7:112829/138‑1 (MQ=255)

                                                                                                  ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        >  8:267118/1‑71 (MQ=255)

                                                                                                  ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGCCCTCCACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAgcgc                                    >  3:415970/1‑139 (MQ=255)

                                                                                                  ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCg                                                      <  1:100647/121‑1 (MQ=255)

                                                                                                  ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAgcgc                                    >  8:406083/1‑139 (MQ=255)

                                                                                                   cGGGGTGCACTCCATACGATCGTTACCGGATAAGGCACTGCGGTCGGGCTGAACGGGGGGTGCGTGCACACAGCCCAGCTTGGAGCGAACGCCCTACACCCACCTGAGATACCAACAGCGTGAGCTATGAGAAAGCGcc                                   <  6:115078/139‑1 (MQ=255)

                                                                                                    gggTTCGACTCAACACGATAGGTCCCGGACAAGGAACCGGGGTCGGGCTTAACGGGGGGTTCGGGCACACAGCCCAGCTTGGAGCGAACGACCAACACCAAAATGAGACACCTACAGCGGGAGCTATGATAAAGCGCCa                                  <  5:76068/139‑1 (MQ=255)

                                                                                                              cAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAAGGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGa                        <  3:37789/139‑1 (MQ=255)

                                                                                                               aaGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                                                 >  5:164702/1‑97 (MQ=255)

                                                                                                               aaGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCg                         >  4:492990/1‑137 (MQ=255)

                                                                                                                 gACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                      <  7:390941/74‑1 (MQ=255)

                                                                                                                    gATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg                                                    <  7:157902/105‑1 (MQ=255)

                                                                                                                     aTAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                                                 <  2:132659/91‑1 (MQ=255)

                                                                                                                      tAGTTACCGGATAAGGCACAGCGGGCGGGCTGAACGGGGGGTGCGGGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCCTCCCGAAGGGAGaa                <  7:348438/139‑1 (MQ=255)

                                                                                                                         ttACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAgg             <  6:475002/139‑1 (MQ=255)

                                                                                                                         ttACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCAAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAgg             >  1:61906/1‑139 (MQ=255)

                                                                                                                           aCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGCCCTACACCCAACTGAGATACCTACAGAGTGAGATATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCg           >  6:213136/1‑139 (MQ=255)

                                                                                                                           aCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCg           >  2:273597/1‑139 (MQ=255)

                                                                                                                             cgtaTAAGGCACAGCGGGCGGGCGGAACGGGGGGTTCGTGCACACCGCCCAGCTTGGAGCGAACGACCTACACCGAAATGAGATACATAAAGCGTGAGCTTTGACAAAGCGCCACGCTTCCCGAAGTGAGAAAGGCGGa         <  8:369457/136‑1 (MQ=255)

                                                                                                                             cGGATAAGGCACAGGGGTCGGGCTGAACGGGGGGTTCGTTCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGa         <  6:150828/139‑1 (MQ=255)

                                                                                                                             cGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGa         <  3:138168/139‑1 (MQ=255)

                                                                                                                             cGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGa         <  7:247998/139‑1 (MQ=255)

                                                                                                                                aTAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                        >  2:481796/1‑41 (MQ=255)

                                                                                                                                    ggCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAAGTAt  >  6:401923/1‑139 (MQ=255)

AGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTAT  >  pSIJ663_pHT315_araE_xy/5070‑5340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`