Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,963,948 G→A intergenic (‑135/+145) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,963,9480GA92.5% 147.8 / ‑5.2 54intergenic (‑135/+145)U712_15285/U712_15290Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (46/3);  minor base C (1/3);  total (48/6)
Fisher's exact test for biased strand distribution p-value = 3.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCAAA  >  CP006881/2963812‑2964073
                                                                                                                                        |                                                                                                                             
atatTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATcacg                                                                                                                             >  4:167414/1‑139 (MQ=255)
      ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGc                                                                                                                       >  4:133858/1‑139 (MQ=255)
      ctcctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTACCGTCATGCAAAATTATCACGTTTGGc                                                                                                                       >  3:267338/1‑139 (MQ=255)
       tcctcctcTTTGCCAACAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATAAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTggg                                                                                                                       >  3:505414/1‑137 (MQ=255)
        cctcctcTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTAGACAGGACAAAAAGTTTCCCTCATGCTAAATTATCACGTTTGGCCa                                                                                                                     >  2:369909/1‑139 (MQ=255)
              ctTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTATCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCATCATGCAAAATTATCACGTTTGGCCATTTTTg                                                                                                               >  6:414614/1‑139 (MQ=255)
                      tCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAACAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGAAAAATTATCACGTTTGGCCATTTTTGAGAAGCTg                                                                                                       >  5:45797/1‑139 (MQ=255)
                        aaTGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCATAAGCATGTTTTATTTACTCAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTgtt                                                                                                     <  7:321975/139‑3 (MQ=255)
                                    ttttATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATaaaaa                                                                                          >  2:135526/1‑138 (MQ=255)
                                         tCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATTCAAAATTATCACGTTTGGCCATTTTTGTCAAGATGATTTACATAAAAAAACCgg                                                                                    >  7:136473/1‑139 (MQ=255)
                                         tCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCgg                                                                                    >  7:13839/1‑139 (MQ=255)
                                           tattgttgtAAGTTCTTAGAAATGTTACATTAAATTACCCAAAAGCATGTTTTATTTACACAGCAAAAAAAGTTTCCCTAATCCAAAATTAACACGTTTGCCCATTTTTGTAAAGCTGATTTACATAAAAAACCCGGCt                                                                                  <  8:465604/136‑1 (MQ=255)
                                              tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGTCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTggg                                                                               >  3:350017/1‑139 (MQ=255)
                                              tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAg                                                                                                          <  4:448817/112‑1 (MQ=255)
                                              tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAg                                                                                                          >  3:448817/1‑112 (MQ=255)
                                              tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTggg                                                                               >  7:255196/1‑139 (MQ=255)
                                              tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTggg                                                                               >  1:109811/1‑139 (MQ=255)
                                              tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTggg                                                                               >  5:403237/1‑139 (MQ=255)
                                              tgttgtCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTggg                                                                               >  4:349092/1‑139 (MQ=255)
                                              tgttgtCAGTTCTTAGACATGTGACATAAAATAAGCCAAAAGCATGTTTAATTTACACAGGACAAAAAGATTCCCTCAAGCAAAATTAACACGTTTGGCCATTTTTGTCAAGCTGATTAACATAAAAAAACAGGCTcgg                                                                               >  2:432702/1‑139 (MQ=255)
                                                   tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcagc                                                                          >  3:396705/1‑139 (MQ=255)
                                                   tCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTcag                                                                           >  3:177757/1‑138 (MQ=255)
                                                       ttCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCTATTTTTGTCAAGCTGATTTACATAAAAAAACAGGCTGGGTCAGCAGcc                                                                      >  1:441697/1‑139 (MQ=255)
                                                            aGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCTGCAGCCGGTc                                                                  >  2:240142/1‑138 (MQ=255)
                                                                 tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCCCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCTGCAGCCGGTCGAAACg                                                            >  8:11242/1‑139 (MQ=255)
                                                                 tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGTACAGCAGCCGGTCCAAAc                                                             >  7:394950/1‑138 (MQ=255)
                                                                 tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCTGTctctta                                                             >  3:121746/1‑133 (MQ=255)
                                                                 tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc                                                             >  7:437923/1‑138 (MQ=255)
                                                                 tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc                                                             >  8:363094/1‑138 (MQ=255)
                                                                 tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc                                                             >  1:56733/1‑138 (MQ=255)
                                                                 tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc                                                             >  1:425833/1‑138 (MQ=255)
                                                                 tgtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATAATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACAGGCTGGGTCAGCAGCCGGTCCAAAc                                                             >  5:33312/1‑138 (MQ=255)
                                                                  gtgACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAAc                                                             >  3:399700/1‑137 (MQ=255)
                                                                     aCATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACgag                                                          >  6:65023/1‑137 (MQ=255)
                                                                        ttAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAgg                                                      >  7:486302/1‑138 (MQ=255)
                                                                                gCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAACCGAGAAGGGATGTAtt                                              >  6:495214/1‑138 (MQ=255)
                                                                                gCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTAtt                                              >  6:143754/1‑138 (MQ=255)
                                                                                gCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTAtt                                              >  6:502750/1‑138 (MQ=255)
                                                                                 ccAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGATGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTc                                             >  4:63416/1‑138 (MQ=255)
                                                                                      accATTTTTTATTTACAACGGACAAAAATTTTCCCCAATTCAAAATTAACCCGTTTGGCCATTTTTGTCAAGGTTTTTTAAAAAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGaa                                       <  8:394950/137‑1 (MQ=255)
                                                                                          tGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAacacc                                   >  1:147302/1‑138 (MQ=255)
                                                                                          tGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACt                                   >  3:202053/1‑139 (MQ=255)
                                                                                           gTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACtt                                  >  5:305007/1‑139 (MQ=255)
                                                                                                ttaaacacAGTACAAAACGTTTCCATCATAAAAAATTATCCGGTTGGGCTTTTTTTTTCTAGTTTTTTAACATAAAAACACCGGCTGGGTCAGCTGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTAc                              <  8:486302/134‑1 (MQ=255)
                                                                                                     cacaGGACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTaa                         >  6:355481/1‑138 (MQ=255)
                                                                                                          gACAAAAAGTTTCCCTCATGCAAAATTATCACGTTTGGCCAGTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAgaa                    >  8:472306/1‑136 (MQ=255)
                                                                                                             aaaaaTTTACCCTCATCAAAATTTATCCCGTTTGGCATTTTTTGTAAAGGTTATTTAAAAAAAAAACCCGGCTGGGTCACCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAgt                <  3:133858/139‑1 (MQ=255)
                                                                                                                    ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAttt         >  3:216323/1‑139 (MQ=255)
                                                                                                                    ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt          >  7:31390/1‑138 (MQ=255)
                                                                                                                    ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt          >  3:14923/1‑138 (MQ=255)
                                                                                                                    ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt          >  2:346366/1‑138 (MQ=255)
                                                                                                                    ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt          >  2:234595/1‑138 (MQ=255)
                                                                                                                    ttCCCTCATGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCAtt          >  1:34978/1‑138 (MQ=255)
                                                                                                                            tGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCaaa  >  5:286104/1‑138 (MQ=255)
                                                                                                                            tGCAAAATTATCACGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAAACGGCTGGGTCAGCTGCCGGTCAAAACGAGAAGGGATGTATTCAACGAACACTTATACTGTAACAGCACAGTTTCATTTATTCaaa  >  7:365103/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                             
ATATTTCTCCTCCTCTTTGCCATCAATGTCCGATTGTTTTATCTTATGTTGTCAGTTCTTAGACATGTGACATTAAATTAGCCAAAAGCATGTTTTATTTACACAGGACAAAAAGTTTCCCTCATGCAAAATTATCGCGTTTGGCCATTTTTGTCAAGCTGATTTACATAAAAAAACCGGCTGGGTCAGCAGCCGGTCCAAACGAGAAGGGATGTATTCAACGAACACTTTTACTGTAACAGCACAGTGTCATTTATTCAAA  >  CP006881/2963812‑2964073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑