Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP006881	1,663,448	Δ9 bp	11.0%	coding (229‑237/795 nt)	U712_08555 →	Flagellar basal‑body rod protein flgG

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP006881	= 1663447	50 (0.820)	6 (0.100)	6/220	NT	11.0%	coding (228/795 nt)	U712_08555	Flagellar basal‑body rod protein flgG
	?	CP006881	1663457 =	50 (0.870)					coding (238/795 nt)	U712_08555	Flagellar basal‑body rod protein flgG

AATACAGTAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGATATGGTAAGCCAAACGATTGCCGGCGGTTCTGCTGCTGGTGCAACAATCGGCGGAACAAACTCTAAGCAAATCGGTTTAGGTTCATCATCTGGCACAATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP006881/1663307‑1663447 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acaattCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGACAGCTTATACAAGAGCAGGGAACTTCTATTTAGATAATACTGGAA  >  CP006881/1663457‑1663592                                                                                                                                                                                                                                                                                       AATACAGTAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGATATGGTAAGCCAAACGATTGCCGGCGGTTCTGCTGCTGGTGCAACAATCGGCGGAACAAACTCTAAGCAAATCGGTTTAGGTTCATCATCTGGCACAA                                                                                                                                            <  4:140538/139‑1  ATACAGTAGGATTCAAAAAGAGCCGTGTGACCTTTAACGATATTGTAAGCCAAACGATTGCCGGCGGTTCTGCTGCTGGTGCAACAATCGGCGGAACAAACTCTAAGCAAATCGGTTTAGGGTCATCATCTGGCACAAT                                                                                                                                           <  5:7541/139‑1        TAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGATTTGGTAAGCCAAACGATTGCCGGCGGTTCTGCTGCTGGTGGCACAATCGGCGGACCAAACACTAAGCAAAACGGTTTTTGTTCATCATCTGGCACAATTCCTT                                                                                                                                      >  4:143062/1‑138        TAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGATATGGTAATCCAAACGATTGCCGGCGGTTCTTCTGCTGGTGGCACAATCGGCGGCACAAACTCTAAGCAAATCCGGTTAGGTTCATGATCTGGCACAATTCATT                                                                                                                                      >  8:50781/1‑138        TAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGATATGGTAAGCCAAACGATTGCCGGCGGTTCTGCTGCTGGTGCAACAATAGGCGGAACAAACTCTAAGCAAATCGGTTTAGGTTCATCATCTGGCACAATTCATT                                                                                                                                      >  7:169501/1‑138        TAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGATATGGTAAGCC‑AACGATTGCCGGCGGTTCTGCTGCTGGTGCAACAATCGGCGGAACAAACTCTAAGCAAATCGGTTTAGGTTCATCATCTGGCACAATTCATTC                                                                                                                                     >  7:266047/1‑138                AAAAAGAGCCGTGTGACCTTTAAAGATATGGTAAGCCAAACGATTGCCGGCGGTTCTGCTGCTGGTGCAACAATCGGCGGAACAAACTCTAAGCAAATAGGTTTAGGTTCATCATCTGGCACAATTCATTCAACAAGCG                                                                                                                             >  7:240142/1‑139                  AAAGAGCCGTGTGACCTTTAAAGATATGGTAAGCCAAACGATTGCCGGCGGTTCTGCTGCTGGTGCAACAATCGGCGGAACAAACTCTAAGCAAATCGGTTTAGGTTCATCATCTGGCACAATTCATTCAACAAGCGCG                                                                                                                           <  5:28704/139‑1                                                             GCCGGCGGTTCTGCTGCTGGTGCAACAATCGGCGGAACAAACTCTAAGCAAATCGGTTTAGGTTCATCATCTGGCACAATTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCG                                                                                >  5:67018/1‑139                                                                                              GGAACAAACTCTAAGCAAATCGGTTTAGGTTCATCATCTGGCACAATTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGA                                               <  7:50781/139‑1                                                                                                               AATCGGTTTAGGTTCATCATCTGGCACAATTCATTCAACACGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGACAGCTTATACAAGAGCA                              <  3:241905/139‑1                                                                                                                       TAGGTTCATCATCTGTCACAATTCATTCAACAAGCGCGACACAAAGCACAGGAATAACACTTGATTTAGCAATTGACGGCGACGTTTACTTCCGAAATGATACAGGTGACGGGACAGCTTATACAAGAGCAGGGAACT                       <  8:266047/138‑1                                                                                                                                           ATTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGACAGCTTATACAAGAGCAGAGAACTTCTATTTAGATAATACTGGAA  >  4:76247/1‑139                                                                                                                                            TTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGACAGCTTATACAAGAGCA                              <  3:145531/110‑1                                                                                                                                            TTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGACAGCTTATACAAGAGCA                              <  7:200375/110‑1                                                                                                                                            TTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGACAGCTTATACAAGAGCA                              >  4:145531/1‑110                                                                                                                                            TTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGACAGCTTATACAAGAGCA                              >  8:200375/1‑110                                                                                                                                            TTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGA                                               >  4:162772/1‑93                                                                                                                                            TTCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGA                                               <  3:162772/93‑1                                                                                                                                                                                                                                                                                       AATACAGTAGGATTCAAAAAGAGCCGTGTGACCTTTAAAGATATGGTAAGCCAAACGATTGCCGGCGGTTCTGCTGCTGGTGCAACAATCGGCGGAACAAACTCTAAGCAAATCGGTTTAGGTTCATCATCTGGCACAATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP006881/1663307‑1663447 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acaattCATTCAACAAGCGCGACACAAAGCACAGGAAGAACACTTGATTTAGCAATTGACGGCGACGGTTACTTCCGAATTGATACAGGTGACGGGACAGCTTATACAAGAGCAGGGAACTTCTATTTAGATAATACTGGAA  >  CP006881/1663457‑1663592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.