Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,576 G→A 100% G130G (GGG→GGA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,5760GA100.0% 199.5 / NA 60G130G (GGG→GGA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/23);  total (37/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATT  >  CP006881/3723448‑3723705
                                                                                                                                |                                                                                                                                 
ttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                                                          >  8:243055/1‑138 (MQ=255)
  ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCTGCAATATAATCGTCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                                        <  7:51241/138‑1 (MQ=255)
      agaacctataCCGTGCTCCGGAAAAATATCTGCTGGCGCGACTCCCTATACTTGTGGATGTCAAGCTATGCCAGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                   <  4:130535/133‑1 (MQ=255)
       tCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                   <  8:176880/138‑1 (MQ=255)
       tCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                   <  7:289743/138‑1 (MQ=255)
         acctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                                        >  8:203248/4‑131 (MQ=255)
         acctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                                        <  7:203248/128‑1 (MQ=255)
             ataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCa                                                                                                            >  6:331511/1‑139 (MQ=255)
             ataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCa                                                                                                            >  3:235008/1‑139 (MQ=255)
              taCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCa                                                                                                            <  3:310179/138‑1 (MQ=255)
               aCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCAtt                                                                                                          >  3:32940/1‑139 (MQ=255)
                   cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                                                      >  5:328671/1‑139 (MQ=255)
                   cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                                                      >  3:137446/1‑139 (MQ=255)
                   cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                                                      >  8:46601/1‑139 (MQ=255)
                     cTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtttt                                                                                                    >  7:117398/1‑139 (MQ=255)
                        cggaaaaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTg                                                                                                  <  7:46601/135‑1 (MQ=255)
                                 atcagCTGGCGCGACTCCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTgg                                                                                        <  6:328671/135‑1 (MQ=255)
                                         gcgcGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTATGCCATCCTGAGCAGCGCGACGGGACATTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGca                                                                                 <  5:313700/138‑1 (MQ=255)
                                                          tgtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGccgccggc                                                               >  7:326963/1‑139 (MQ=255)
                                                           gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                   >  4:219066/1‑86 (MQ=255)
                                                           gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                                   <  3:219066/86‑1 (MQ=255)
                                                           gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgccgg                                                                <  8:326963/137‑3 (MQ=255)
                                                           gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTAATTTGGCAGAGCACAAAATGGAGCAGCCTGCt                                                              >  8:137308/1‑139 (MQ=255)
                                                           gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCAGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCt                                                              >  4:592/1‑139 (MQ=255)
                                                           gaggATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCAGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCCGCt                                                              >  3:210301/3‑139 (MQ=255)
                                                            tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCa                                                                                                            <  6:174563/92‑1 (MQ=255)
                                                            tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCa                                                                                                            >  5:174563/1‑92 (MQ=255)
                                                            tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCGGTTTTGATTCATTTTGCAGAGCACAAAATTGAGCCGCCGGCtt                                                             >  5:33251/1‑139 (MQ=255)
                                                            tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                                             >  2:127951/1‑139 (MQ=255)
                                                            tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                                             >  2:277978/1‑139 (MQ=255)
                                                            tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                                             >  4:266781/1‑139 (MQ=255)
                                                            tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                                             >  3:54549/1‑139 (MQ=255)
                                                              gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                                           >  4:283975/1‑139 (MQ=255)
                                                              gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                                           >  3:128188/1‑139 (MQ=255)
                                                              gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAGTCTGTTTGCCATCCTGATCAGCGCGACGGGAATTTTTGAGGAGCCTTCTGTTACTTCCTTTTTTATTCATTTTGGAGAGCACAAAATGGCGGCTCCGGCTTcc                                                           >  5:264047/1‑139 (MQ=255)
                                                                tGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCg                                                          >  3:31158/1‑138 (MQ=255)
                                                                    aaGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTGCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg                                                     >  1:307770/1‑139 (MQ=255)
                                                                    aaGCTAGCCCGGCAATTTACTCGGCGCCCTTCTGTTTGCCATCGTCACCCGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg                                                     <  3:202615/139‑1 (MQ=255)
                                                                         aTGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGca                                                                                 <  1:138693/106‑1 (MQ=255)
                                                                         aTGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGca                                                                                 >  2:138693/1‑106 (MQ=255)
                                                                          tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                <  3:266781/138‑1 (MQ=255)
                                                                          tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTACGAAATGttttt                                                >  5:34478/1‑138 (MQ=255)
                                                                          tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCGGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATTGAGCCCCCCGCGTCCCAATTGttttt                                                >  4:178222/1‑138 (MQ=255)
                                                                           gacgccAATTAAATCGGCGCCATTCTGTTTGCCATCCGGATCCGCGCGACGGGACTTGTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCa                                              <  6:176311/134‑1 (MQ=255)
                                                                           gCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGCCGGGACGTTTTGAGGAGCCGTCGGTTCATTCCTTTTTGATTGATTATGCAGAGCACAAAATGGCGCCGCCGGCTAGCGAATTGtgttt                                                >  2:172946/1‑137 (MQ=255)
                                                                                   tttAATCGGCGCCATGCTGTTTGCAATCCTTATCTGCGCGACGGGACTTTTTGAGGAGCCTTATGTTCATTCCTTTTTGATTCATTTGGCAGAGCCCAAAATGTAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                                      <  4:235008/139‑1 (MQ=255)
                                                                                       aTCGGCGCCATTCTGTTTGCAATCCTGATCAGCGCGAGGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGTCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtg                                   <  7:137308/138‑1 (MQ=255)
                                                                                                      tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgt                   >  3:99512/1‑139 (MQ=255)
                                                                                                      tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgt                   >  5:298519/1‑139 (MQ=255)
                                                                                                      tatGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCACAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgt                     <  5:236491/135‑1 (MQ=255)
                                                                                                         gCCATCCTTAGCAGCTCGACGGGACTTTTGGAGGAGCCTTCTGTTCATTACTTTTTGATTCATTTGGCAGAGAACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc                <  8:121373/139‑1 (MQ=255)
                                                                                                         gCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg                                                     <  7:181665/102‑1 (MQ=255)
                                                                                                         gCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg                                                     >  8:181665/1‑102 (MQ=255)
                                                                                                          ccATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc                <  4:137446/138‑1 (MQ=255)
                                                                                                              ccTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGcc           >  2:57384/2‑139 (MQ=255)
                                                                                                                tGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTAATTCCTTTTTGATTCATTTGGCAGATCACAAAATGGATCCGCCGGCTTACGAATTGTTTTTCAGAGGAATGCTGTTCAATTGGCTTGTGTGCGTCGCCtg         >  2:228652/1‑138 (MQ=255)
                                                                                                                       cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                <  2:14280/93‑1 (MQ=255)
                                                                                                                       cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                                >  1:14280/1‑93 (MQ=255)
                                                                                                                       cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt  >  7:33291/1‑139 (MQ=255)
                                                                                                                       cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAAATGTTTTTCCGAGGAATGCAGTTCAAATTGCTTGCTTGCCACGCCTTTTTCAtt  >  6:18422/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                 
TTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATT  >  CP006881/3723448‑3723705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑