| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,723,642 | C→G | 100% | P152P (CCC→CCG) | U712_19195 → | putative transporter ywcJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,723,642 | 0 | C | G | 97.4% | 209.8 / ‑5.8 | 63 | P152P (CCC→CCG) | U712_19195 | putative transporter ywcJ |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base G (38/21); total (42/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCAT > CP006881/3723506‑3723773 | tgtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGccgccggc > 7:326963/1‑139 (MQ=255) gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTAATTTGGCAGAGCACAAAATGGAGCAGCCTGCt > 8:137308/1‑139 (MQ=255) gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCAGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCt > 4:592/1‑139 (MQ=255) gaggATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCAGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCCGCt > 3:210301/3‑139 (MQ=255) tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCGGTTTTGATTCATTTTGCAGAGCACAAAATTGAGCCGCCGGCtt > 5:33251/1‑139 (MQ=255) tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt > 4:266781/1‑139 (MQ=255) tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt > 3:54549/1‑139 (MQ=255) tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt > 2:127951/1‑139 (MQ=255) tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt > 2:277978/1‑139 (MQ=255) gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc > 4:283975/1‑139 (MQ=255) gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc > 3:128188/1‑139 (MQ=255) gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAGTCTGTTTGCCATCCTGATCAGCGCGACGGGAATTTTTGAGGAGCCTTCTGTTACTTCCTTTTTTATTCATTTTGGAGAGCACAAAATGGCGGCTCCGGCTTcc > 5:264047/1‑139 (MQ=255) tGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCg > 3:31158/1‑138 (MQ=255) aaGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTGCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg > 1:307770/1‑139 (MQ=255) aaGCTAGCCCGGCAATTTACTCGGCGCCCTTCTGTTTGCCATCGTCACCCGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg < 3:202615/139‑1 (MQ=255) tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt < 3:266781/138‑1 (MQ=255) tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTACGAAATGttttt > 5:34478/1‑138 (MQ=255) tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCGGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATTGAGCCCCCCGCGTCCCAATTGttttt > 4:178222/1‑138 (MQ=255) gacgccAATTAAATCGGCGCCATTCTGTTTGCCATCCGGATCCGCGCGACGGGACTTGTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCa < 6:176311/134‑1 (MQ=255) gCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGCCGGGACGTTTTGAGGAGCCGTCGGTTCATTCCTTTTTGATTGATTATGCAGAGCACAAAATGGCGCCGCCGGCTAGCGAATTGtgttt > 2:172946/1‑137 (MQ=255) tttAATCGGCGCCATGCTGTTTGCAATCCTTATCTGCGCGACGGGACTTTTTGAGGAGCCTTATGTTCATTCCTTTTTGATTCATTTGGCAGAGCCCAAAATGTAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg < 4:235008/139‑1 (MQ=255) aTCGGCGCCATTCTGTTTGCAATCCTGATCAGCGCGAGGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGTCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtg < 7:137308/138‑1 (MQ=255) tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgt > 5:298519/1‑139 (MQ=255) tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgt > 3:99512/1‑139 (MQ=255) tatGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCACAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgt < 5:236491/135‑1 (MQ=255) gCCATCCTTAGCAGCTCGACGGGACTTTTGGAGGAGCCTTCTGTTCATTACTTTTTGATTCATTTGGCAGAGAACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc < 8:121373/139‑1 (MQ=255) gCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg < 7:181665/102‑1 (MQ=255) gCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTg > 8:181665/1‑102 (MQ=255) ccATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc < 4:137446/138‑1 (MQ=255) ccTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGcc > 2:57384/2‑139 (MQ=255) tGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTAATTCCTTTTTGATTCATTTGGCAGATCACAAAATGGATCCGCCGGCTTACGAATTGTTTTTCAGAGGAATGCTGTTCAATTGGCTTGTGTGCGTCGCCtg > 2:228652/1‑138 (MQ=255) cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt > 1:14280/1‑93 (MQ=255) cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt < 2:14280/93‑1 (MQ=255) cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAtt > 7:33291/1‑139 (MQ=255) cgcgACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAAATGTTTTTCCGAGGAATGCAGTTCAAATTGCTTGCTTGCCACGCCTTTTTCAtt > 6:18422/1‑139 (MQ=255) cgggacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCATAGGAATGCTGTGCAATTGGCTTGTGTGACTCGCCTTTTTAATTCCAAt > 8:60320/6‑139 (MQ=255) cgggacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCACAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAAt < 8:117398/134‑1 (MQ=255) cgggacTTTTTGAGGAGCCTTCTGTTCATTCCTGTTTGATTCATTTGGCAGAGCCCAAAATGGAGCCGCCGGCTTCCGACTTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTGCCTTCCAAt > 8:2790/6‑139 (MQ=255) gggacTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGTAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATg < 7:243055/135‑1 (MQ=255) acTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGtct < 1:277978/138‑1 (MQ=255) cTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGtct < 1:127951/138‑1 (MQ=255) ttGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTctctca < 4:54549/138‑3 (MQ=255) ggagcCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaaggg > 2:214815/1‑139 (MQ=255) agccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggga < 3:283975/136‑1 (MQ=255) agccTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAaagggga < 4:128188/136‑1 (MQ=255) gccTTCTGTTCATTCCTTTTTGATTAATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGATGTGCAATTGGATTGGGTTCCTAGCCTGTTTAATTCCAATGTATGTAAaagtggaa > 7:123395/3‑139 (MQ=255) gTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa < 7:60320/138‑1 (MQ=255) ttCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCa < 3:176918/137‑1 (MQ=255) aTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGGTTCCGAATTGTTTTTCAGAGGAAAGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATCTCTCTCAAAGGGGAAGGAGCAAAGct > 3:104514/1‑139 (MQ=255) aTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTAAGAGGAAAGCGGTGCAATTGGCGTGGGTGCCTCCCCTTTTTCATTCCCATGTCTCACAAAGTGGAAGGAGCaaacca > 4:191210/1‑136 (MQ=255) tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc > 4:302290/1‑91 (MQ=255) tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc < 3:302290/91‑1 (MQ=255) tCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGcttt > 6:55035/1‑137 (MQ=255) tttGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt > 7:85639/1‑95 (MQ=255) tttGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCtttt < 8:85639/95‑1 (MQ=255) agCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGttt > 2:52603/1‑138 (MQ=255) agCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTACAATGTCTCTAAAAGGGGAAGGAGCAAAGCTTTTTACCATTATGCTTTTAGtttt > 8:130228/1‑139 (MQ=255) cacaAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCGCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCt > 1:219533/1‑139 (MQ=255) cacaAAATGGAGCCGCCGGCTTCCCAATAGTTTTTAAGAGGAATTCTGTTCAATTGGGATGTGTGCCTCGCCGTTTTAATTCCAATGTCTCTGAAAGGGGAATGAGCAAATCTTTTTACCAAGATTCTTTTGGttttga > 8:118570/1‑137 (MQ=255) ggAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGcttcttt > 5:159343/1‑138 (MQ=255) aGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATTCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTGATTCCAATGTCTCTCAAAGGTGAAGGAGCAAAGCTTTTTAACATGATGCTGTTGGTTGTATTCTTCTTTAt > 1:301317/1‑139 (MQ=255) aGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTAt > 7:312572/1‑139 (MQ=255) aGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTAt > 5:162071/1‑139 (MQ=255) aGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTATACCATCATGCTTTTCGTATTCTGCTTCTTTAt > 6:84964/1‑139 (MQ=255) | TGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCAT > CP006881/3723506‑3723773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |