Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 632,571 A→T 5.1% M313L (ATG→TTG)  U712_03090 → putative glucitol transport protein gutA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881632,5710AT 5.1% 240.6 / 3.9 79M313L (ATG→TTG) U712_03090putative glucitol transport protein gutA
Reads supporting (aligned to +/- strand):  ref base A (39/36);  new base T (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

GATGGTGTATTATTTTACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAACGCCTTTAAATACGCTTGCTTGGGGAA  >  CP006881/632435‑632709
                                                                                                                                        |                                                                                                                                          
gATGGTGTATTATTTTACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATg                                                                                                                                          <  4:10962/139‑1 (MQ=255)
 aTGGTGTATTATTTTACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGa                                                                                                                                         >  7:247903/1‑139 (MQ=255)
  tGGTGTATTATTTTACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGAt                                                                                                                                        >  3:293585/1‑139 (MQ=255)
  tGGTGTATTATTTTACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGAt                                                                                                                                        >  3:225856/1‑139 (MQ=255)
  tGGTGTATTATTTTACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGAt                                                                                                                                        >  3:119026/1‑139 (MQ=255)
  tGGTGTATTATTTTACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGAt                                                                                                                                        >  2:186625/1‑139 (MQ=255)
            ttttACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACgg                                                                                                                                >  4:10584/1‑137 (MQ=255)
               tACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGct                                                                                                                           >  2:117193/1‑139 (MQ=255)
                      aaCGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCTGAAAACAAGTCATGATTATCACGGCAGCTCTATCGc                                                                                                                    >  2:270089/1‑139 (MQ=255)
                      aaCGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGc                                                                                                                    >  2:38950/1‑139 (MQ=255)
                             cccATAAAGCGTTCGGGTGTATGGGCCCCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTACTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCGATCGCTTAtgta                                                                                                             >  5:309360/2‑136 (MQ=255)
                             aCCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTc                                                                                                             >  5:194973/1‑139 (MQ=255)
                             aCCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTACTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTc                                                                                                             >  5:314058/1‑139 (MQ=255)
                               cATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCt                                                                                                            >  7:178738/1‑138 (MQ=255)
                                 tAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATg                                                                                                                                          >  8:3618/1‑106 (MQ=255)
                                 tAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATg                                                                                                                                          <  7:3618/106‑1 (MQ=255)
                                 tAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTAt                                                                                                         >  6:87795/1‑139 (MQ=255)
                                 tAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTAt                                                                                                         >  4:130046/1‑139 (MQ=255)
                                  acacagTTCGTGTTTATGGGCACCGTTTTTTTTTTCGGGGCAGCGCGGATCCGCAATTTGTTAATTCCTTTCTTTGCTGAGATGTGGGGCCGAAAACAAGTCATCTTAATCACGGCAGCTCTATCGCTTATTTCTTATg                                                                                                        <  6:309360/135‑1 (MQ=255)
                                  aaaGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATg                                                                                                        >  5:238137/1‑139 (MQ=255)
                                  aaaGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATg                                                                                                        >  4:52301/1‑139 (MQ=255)
                                  aaaGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATg                                                                                                        >  8:231182/1‑139 (MQ=255)
                                  aaaGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTACTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATg                                                                                                        <  6:314058/139‑1 (MQ=255)
                                   aaGTGTTGGTGTTTATGGGAACCTTTTTTTTTTTCGGTGCAGCGATGAGAAGAAATTTGTTTATTCCTTTTTTTCCTGAGAAGGGGGGCACAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATg                                                                                                        <  6:214874/138‑1 (MQ=255)
                                   aaGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGc                                                                                                       <  3:130046/139‑1 (MQ=255)
                                        ttCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGTCAGCTCTATCGCTTATTTATTATGACGGcc                                                                                                  >  3:90237/1‑139 (MQ=255)
                                        ttCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCATTTCTTTTCTGAGAAGTGGGGCAGAAAACAACTCATGATTATCACGCCAGCTCTATCGCTTATTTCTTATGCCGGc                                                                                                   >  5:303392/1‑138 (MQ=255)
                                        ttCGTGTTTATGGGCACCGTTTTATTCTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGcc                                                                                                  >  1:93652/1‑139 (MQ=255)
                                        ttCGTGTTTATGGGCACCGTATTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCGTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCCCGGCAGCGCGATCTCTTATTTCTTATGCCGGcc                                                                                                  >  3:295035/1‑139 (MQ=255)
                                         tCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGctct                                                                                                                         <  2:71190/115‑1 (MQ=255)
                                         tCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGctct                                                                                                                         >  1:71190/1‑115 (MQ=255)
                                         tCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCt                                                                                                 >  8:298717/1‑139 (MQ=255)
                                          ggggtTTATGGGAACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCGTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTAATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCt                                                                                                 <  4:90237/135‑1 (MQ=255)
                                                 aTGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGAAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCAtttt                                                                                          <  8:308307/138‑1 (MQ=255)
                                                    ggCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGTTATTTGTTTTTTCCTTTTTTTTCTGAGAAGTGGGGCAGAAAACATGTCATGTTTTTCACGGCAGCTCTATCGCTTATTTCTTTTGCCGGCCTGCATTTTACg                                                                                       <  6:303392/138‑1 (MQ=255)
                                                    ggCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACg                                                                                       <  7:198839/138‑1 (MQ=255)
                                                    ggCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACg                                                                                       <  5:305088/138‑1 (MQ=255)
                                                    ggCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACg                                                                                       <  1:117193/138‑1 (MQ=255)
                                                    ggCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACg                                                                                       <  4:293585/138‑1 (MQ=255)
                                                      cACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTAt                                                                                                                                     >  7:273506/1‑90 (MQ=255)
                                                      cACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTAt                                                                                                                                     <  8:273506/90‑1 (MQ=255)
                                                       aCCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCg                                                                                    >  4:22047/1‑138 (MQ=255)
                                                        ccGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  <  6:194973/139‑1 (MQ=255)
                                                        ccGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  <  3:22047/139‑1 (MQ=255)
                                                        ccGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  <  3:10584/139‑1 (MQ=255)
                                                        ccGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  <  7:231182/139‑1 (MQ=255)
                                                        ccGGTTTATTTTTCGGGGCAGCGCTGAGCAGCAATTGGTTTATTCCTTTCTTTGCTGACACGGGGGGCAGAAAACAAGGCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  <  2:166115/139‑1 (MQ=255)
                                                        aggTTTTATTTTTGGGGGCAGCGCTGATCAGCATTTTGTTTATTGCTTTCTTTTCTGAGAAGGGGGGCACAAAACAAGTCATGAATATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  <  4:3801/137‑1 (MQ=255)
                                                        actttttATTTTGCGGGGCAGCGCTGATAAGCAATTTGTTTATTCCTTTCTTTTCTGAGAATTGGGGAAGAAAACTAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  <  4:25143/136‑1 (MQ=255)
                                                         cGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTCCGCCGTAt                                                                                 >  6:199521/1‑139 (MQ=255)
                                                           ttttATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCAtt                                                                                            >  4:4206/1‑126 (MQ=255)
                                                           ttttATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCAtt                                                                                            <  3:4206/126‑1 (MQ=255)
                                                           ttttATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTAttc                                                                               >  8:324772/1‑139 (MQ=255)
                                                                     cGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  >  1:120462/1‑126 (MQ=255)
                                                                     cGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTa                                                                                  <  2:120462/126‑1 (MQ=255)
                                                                     cGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTcct                                                                     >  3:3801/1‑138 (MQ=255)
                                                                          cAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACtcat                                                                <  7:298717/139‑1 (MQ=255)
                                                                                  aTCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTg                                                                                                <  1:232310/99‑1 (MQ=255)
                                                                                  aTCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTg                                                                                                >  2:232310/1‑99 (MQ=255)
                                                                                  aTCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTGCACtcattttcattt                                                        >  1:237010/1‑139 (MQ=255)
                                                                                         aTTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCtttt                                                 <  5:87795/139‑1 (MQ=255)
                                                                                         aTTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCtttt                                                 <  8:311981/139‑1 (MQ=255)
                                                                                         aTTTGTTTATTCCTTTATTTTCTGAGAAGGGGGGCAGAAAACAAGTCATGATTAAGACGGCAGCGCTATCGCTTATTTCATATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCtttt                                                 <  1:38950/139‑1 (MQ=255)
                                                                                          tttGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTTGTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCAGGCATTTTCATTTGGCTTTTc                                                >  4:56424/1‑139 (MQ=255)
                                                                                                 aTTCCTTTCTTTTCTGAGAAGTGGGGCTGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGTCTTTTCGCTTCgg                                         >  2:53168/1‑139 (MQ=255)
                                                                                                       ttcttttctGAGAAGTGGGGCAGAAAACAAGTCTTGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACg                                                                                       >  2:47260/1‑87 (MQ=255)
                                                                                                       ttcttttctGAGAAGTGGGGCAGAAAACAAGTCTTGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACg                                                                                       <  1:52259/87‑1 (MQ=255)
                                                                                                       ttcttttctGAGAAGTGGGGCAGAAAACAAGTCTTGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACg                                                                                       >  2:52259/1‑87 (MQ=255)
                                                                                                       ttcttttctGAGAAGTGGGGCAGAAAACAAGTCTTGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACg                                                                                       <  1:47260/87‑1 (MQ=255)
                                                                                                              ctGAGAAGTGGGGCAGAAAACAAGTCATGATTCTCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACaa                             <  4:225856/138‑1 (MQ=255)
                                                                                                              ctGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACaa                             <  3:52301/138‑1 (MQ=255)
                                                                                                              ctGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACaa                             <  8:247903/138‑1 (MQ=255)
                                                                                                              ctGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACaa                             <  7:144522/138‑1 (MQ=255)
                                                                                                                 agaAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAAc                            <  6:238137/136‑1 (MQ=255)
                                                                                                                 agaAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAACGcc                         <  1:186625/139‑1 (MQ=255)
                                                                                                                      tGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTACACTCATTTTCATTTGGCTTTTCGCTTCGGCATTTTTCAGAACGCCTTTa                     >  3:37815/1‑138 (MQ=255)
                                                                                                                        gggCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAACGCCTTTaaa                   >  2:198001/1‑138 (MQ=255)
                                                                                                                                     gTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAACGCCTTTAAATACGCTTGCTTggg     >  1:135420/1‑139 (MQ=255)
                                                                                                                                      tCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAACGCCTTTAAATACGCTTGCTTgggg    <  2:93652/139‑1 (MQ=255)
                                                                                                                                        aTGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAACGCCTTTAAATACGCTTGCTTGGGGaa  >  7:268012/1‑139 (MQ=255)
                                                                                                                                        aTGATTATCACGGCAGCTCTATCGCTTATTTCTTATGACGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAACGCCTTTAAATACGCTTGCTTGGGGaa  <  6:326942/139‑1 (MQ=255)
                                                                                                                                        aTGATTATCACGGCAGCTCTATCGCTTATTTCTTATGACGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGAGTTTTCACAACGCCTTTAAATACGCTTGCTTGGGGaa  <  4:295035/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GATGGTGTATTATTTTACGTATAACGTAAACCATAAAGAGTTCGTGTTTATGGGCACCGTTTTATTTTTCGGGGCAGCGCTGATCAGCAATTTGTTTATTCCTTTCTTTTCTGAGAAGTGGGGCAGAAAACAAGTCATGATTATCACGGCAGCTCTATCGCTTATTTCTTATGCCGGCCTGCATTTTACGCCGTATTCTTCAATTCCACTCATTTTCATTTGGCTTTTCGCTTCGGGATTTTTCACAACGCCTTTAAATACGCTTGCTTGGGGAA  >  CP006881/632435‑632709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑