Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,724,849	A→T	100%	intergenic (‑23/+17)	U712_19200 ← / ← U712_19205	SacPA operon antiterminator/Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,849	0	A	T	100.0%	155.8 / NA	47	intergenic (‑23/+17)	U712_19200/U712_19205	SacPA operon antiterminator/Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/27);  total (20/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTT  >  CP006881/3724715‑3724981                                                                                                                                       |                                                                                                                                     tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                                                                                                                                  >  7:1004/1‑139 (MQ=255) tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                                                                                                                                  >  7:195486/1‑139 (MQ=255) tGAGATCATTCTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                                                                                                                                  <  2:38075/139‑1 (MQ=255)       cATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTGATCTCTCCCGCCAATTTGCTTTTGACt                                                                                                                            <  1:81448/139‑1 (MQ=255)        aTTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACt                                                                                                                            <  6:304239/138‑1 (MQ=255)        aTTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACt                                                                                                                            <  3:125993/138‑1 (MQ=255)        aTTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACt                                                                                                                            <  5:230171/138‑1 (MQ=255)          ttttCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTgg                                                                                                                          >  7:156450/1‑138 (MQ=255)                  ttttGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTAc                                                                                                                  <  5:315864/138‑1 (MQ=255)                         aaGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCtttt                                                                                                          <  5:19056/139‑1 (MQ=255)                           gCGATTCCCGGTCCCCTCCCGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGa                                                                                                        <  8:139407/139‑1 (MQ=255)                            cGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGAc                                                                                                       <  7:236807/139‑1 (MQ=255)                              aTTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCCAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg                                                                                                     <  4:273885/139‑1 (MQ=255)                              aTTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg                                                                                                     <  3:272828/139‑1 (MQ=255)                                 cccggTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGcc                                                                                                   <  5:187194/135‑1 (MQ=255)                                 cccggTCCCATCACGATTATTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGcc                                                                                                   <  4:228809/135‑1 (MQ=255)                                      tCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTTACCGCCATGTa                                                                                              >  2:194577/1‑138 (MQ=255)                                         cATCACGATTTTTTCCTGATCATCCACTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCTAGAGGATATCTCTCCCGACAATTTGATATAGAATGGGATTTTACATCGTTTTGACCGACATGTa                                                                                              >  2:109814/1‑135 (MQ=255)                                           tCACGATTTTTTCCTGCTCCTCCTCGTTTCTTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAGCTCTCCCGCCAATTTGCTTTTGACGGGGATTTTACCTCCTTTTGACCGCCCTGTACAGATg                                                                                        <  2:8967/139‑1 (MQ=255)                                                aTTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGc                                                                                   <  8:1004/139‑1 (MQ=255)                                                aTTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAATGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCTATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGc                                                                                   <  3:294061/139‑1 (MQ=255)                                                aTTTTTTACTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGc                                                                                   <  4:278928/139‑1 (MQ=255)                                                      tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                              <  8:156450/138‑1 (MQ=255)                                                      tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                              <  5:301571/138‑1 (MQ=255)                                                      tCCTGATCATCCTCTTTAATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                              <  1:287925/138‑1 (MQ=255)                                                                       tattaACGCCGAATTGTGGTTTAATACTTTATAGATTTGCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                             <  2:89308/138‑1 (MQ=255)                                                                       tattaAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                             <  5:145662/138‑1 (MQ=255)                                                                          taAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTcc                                                         >  4:115503/1‑139 (MQ=255)                                                                             aGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACATCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg                                                      <  8:195486/139‑1 (MQ=255)                                                                                       ttgttTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg                                                      >  4:10099/1‑129 (MQ=255)                                                                                       ttgttTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg                                                      <  3:10099/129‑1 (MQ=255)                                                                                       ttgttTAATACTTTATAGATATTCAAGAGGTTATCTCTCCCTCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCTGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCa                                            <  7:207043/139‑1 (MQ=255)                                                                                         gttTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCGTTGAAGGCTTGCgaa                                          >  1:77764/1‑139 (MQ=255)                                                                                            taatACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACAt                                       >  3:320349/4‑139 (MQ=255)                                                                                                    tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                              <  1:18300/92‑1 (MQ=255)                                                                                                    tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                              >  2:18300/1‑92 (MQ=255)                                                                                                    tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCtttt                               >  5:158514/1‑139 (MQ=255)                                                                                                    tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCttt                                >  1:181213/1‑138 (MQ=255)                                                                                                            ttCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAgcg                       >  5:172027/1‑139 (MQ=255)                                                                                                             tCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGAAAAGATGCGGGCCCGCTCTCTACTGCTTTGGCGATTCCTTGAAACCTATCAAACATCATCTTTTATaaatc                        >  6:11982/1‑135 (MQ=255)                                                                                                              cAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGcgcg                     >  2:53854/1‑139 (MQ=255)                                                                                                                       aTCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTc            >  3:28819/1‑139 (MQ=255)                                                                                                                       aTCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTc            >  3:24512/1‑139 (MQ=255)                                                                                                                              ccGCCAATTTGCTTTTGACTGGGATTTTCCCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                              >  3:140260/1‑66 (MQ=255)                                                                                                                              ccGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                              <  4:140260/66‑1 (MQ=255)                                                                                                                              ccGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCtttct     >  7:111415/1‑137 (MQ=255)                                                                                                                                 ccAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAACCATCTTCTTTTATAAAGCGCGGCGGCGTTCGCGTtgtggg  >  4:202022/1‑137 (MQ=255)                                                                                                                                       |                                                                                                                                     TGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTT  >  CP006881/3724715‑3724981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑