Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,724,902	A→C	100%	A89A (GCT→GCG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,902	0	A	C	100.0%	166.0 / NA	45	A89A (GCT→GCG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/23);  total (22/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGC  >  CP006881/3724768‑3725036                                                                                                                                       |                                                                                                                                       tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     <  8:156450/138‑1 (MQ=255) tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     <  5:301571/138‑1 (MQ=255) tCCTGATCATCCTCTTTAATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     <  1:287925/138‑1 (MQ=255)                  tattaACGCCGAATTGTGGTTTAATACTTTATAGATTTGCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                                                                                    <  2:89308/138‑1 (MQ=255)                  tattaAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                                                                                    <  5:145662/138‑1 (MQ=255)                     taAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTcc                                                                                                                >  4:115503/1‑139 (MQ=255)                        aGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACATCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg                                                                                                             <  8:195486/139‑1 (MQ=255)                                  ttgttTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg                                                                                                             >  4:10099/1‑129 (MQ=255)                                  ttgttTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg                                                                                                             <  3:10099/129‑1 (MQ=255)                                  ttgttTAATACTTTATAGATATTCAAGAGGTTATCTCTCCCTCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCTGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCa                                                                                                   <  7:207043/139‑1 (MQ=255)                                    gttTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCGTTGAAGGCTTGCgaa                                                                                                 >  1:77764/1‑139 (MQ=255)                                       taatACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACAt                                                                                              >  3:320349/4‑139 (MQ=255)                                               tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     <  1:18300/92‑1 (MQ=255)                                               tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     >  2:18300/1‑92 (MQ=255)                                               tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCtttt                                                                                      >  5:158514/1‑139 (MQ=255)                                               tataGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCttt                                                                                       >  1:181213/1‑138 (MQ=255)                                                       ttCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAgcg                                                                              >  5:172027/1‑139 (MQ=255)                                                        tCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGAAAAGATGCGGGCCCGCTCTCTACTGCTTTGGCGATTCCTTGAAACCTATCAAACATCATCTTTTATaaatc                                                                               >  6:11982/1‑135 (MQ=255)                                                         cAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGcgcg                                                                            >  2:53854/1‑139 (MQ=255)                                                                  aTCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTc                                                                   >  3:24512/1‑139 (MQ=255)                                                                  aTCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTc                                                                   >  3:28819/1‑139 (MQ=255)                                                                         ccGCCAATTTGCTTTTGACTGGGATTTTCCCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     >  3:140260/1‑66 (MQ=255)                                                                         ccGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct                                                                                                                                     <  4:140260/66‑1 (MQ=255)                                                                         ccGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCtttct                                                            >  7:111415/1‑137 (MQ=255)                                                                            ccAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAACCATCTTCTTTTATAAAGCGCGGCGGCGTTCGCGTtgtggg                                                         >  4:202022/1‑137 (MQ=255)                                                                                    cTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTttcttc                                                  <  6:158514/138‑1 (MQ=255)                                                                                    cTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTttcttc                                                  <  4:11853/138‑1 (MQ=255)                                                                                           cTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                          <  2:181213/139‑1 (MQ=255)                                                                                           cTGGGATTTTACCTACTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACTTCTTCTTTTATATAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                          <  1:109814/139‑1 (MQ=255)                                                                                              ggATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAg                                       <  1:53854/139‑1 (MQ=255)                                                                                               gATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGc                                      <  4:28819/139‑1 (MQ=255)                                                                                               gATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGc                                      <  4:24512/139‑1 (MQ=255)                                                                                                   ttACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                                                                                    <  2:105616/57‑1 (MQ=255)                                                                                                   ttACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                                                                                    >  1:105616/1‑57 (MQ=255)                                                                                                          ctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTGTGGCGTTCGTGTGTTAGTCCTCGTTGACAGCCCTCCGCCat                            >  8:79951/1‑137 (MQ=255)                                                                                                          ctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGGTTTGTGCTTTTATTCCTCATTCACAGCCCTCCGCCat                            >  7:65004/1‑137 (MQ=255)                                                                                                                  cGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAAc                   <  5:99240/139‑1 (MQ=255)                                                                                                                    ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTgg                                                                                                                       >  5:258971/1‑37 (MQ=255)                                                                                                                    ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTgg                                                                                                                       <  6:258971/37‑1 (MQ=255)                                                                                                                    ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                                                                                    >  4:205480/1‑40 (MQ=255)                                                                                                                    ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa                                                                                                                    <  3:205480/40‑1 (MQ=255)                                                                                                                              aTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTcct       <  1:194577/139‑2 (MQ=255)                                                                                                                                 cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg    <  6:172027/139‑1 (MQ=255)                                                                                                                                 cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTAGCTTTCTGGTTTTATTACACATTACCAGCCCAACGCCAATGATAAAACCAAACGAGTCCagcc    >  6:64368/1‑138 (MQ=255)                                                                                                                                   ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTATGCTTTTATTACTCATTCACAGCCATCCGCCAATCATAAAACGAAACCAGTCCTGCGGc  >  8:52751/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGC  >  CP006881/3724768‑3725036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑