| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 743,700 | G→A | 7.1% | V380I (GTA→ATA) | U712_03585 → | Lipoprotein lipO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 743,700 | 0 | G | A | 7.1% | 182.5 / 2.6 | 56 | V380I (GTA→ATA) | U712_03585 | Lipoprotein lipO |
| Reads supporting (aligned to +/- strand): ref base G (21/31); new base A (2/2); total (23/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01 | |||||||||||
AAATCAAGCGTCAAAACCGAAAAAGAGCTGAAAGAGGTGCTGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTCCAGCATCGCAAGGA > CP006881/743571‑743836 | aaaTCAAGCGTCAAAACCGAAATAGAGCTGAACGAGGCGCTGTCCTTCCTCGATAAGATGAATGATAAGGATGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCt < 5:164819/139‑1 (MQ=255)aaaTCAAGCGTCAAAACCGAAAAAGAGCTGAAAGAGGTGCTGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCt < 4:146575/139‑1 (MQ=255) gCGTCAAAACCGAAAAAGAGCTGAAAGAGGTGCTGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTg > 3:47371/1‑138 (MQ=255) aaaaCAGAAAAAGAGCTGAAAGAGGTGCGGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTTAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGaaaaa < 5:233832/138‑1 (MQ=255) aaaGAGCTGAAAGAGGTGCTGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAAt < 4:40403/139‑1 (MQ=255) aaaGAGCTGAAAGAGGTGCTGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAAt < 4:47371/139‑1 (MQ=255) gCTGAAAGAGGTGCTGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAAtctctc < 5:153198/139‑1 (MQ=255) tGCTGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCa > 5:134684/1‑139 (MQ=255) tccttcctCGATAAGATGATTGATAAGGAAGCGCAGATTCTCACGAACACCGCCGGGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa < 1:10650/137‑1 (MQ=255) tccttcctCGATAAGATGAGTGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa < 3:334270/137‑1 (MQ=255) tccttcctCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa < 3:113560/137‑1 (MQ=255) tccttcctCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa < 7:289726/137‑1 (MQ=255) tccttcctCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa < 5:144538/137‑1 (MQ=255) tccttcctCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAg > 1:38596/1‑139 (MQ=255) gATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGca < 5:729/138‑1 (MQ=255) gATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGca < 6:146516/138‑1 (MQ=255) aTAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGcaca < 6:134684/139‑1 (MQ=255) aTAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGca > 5:297112/1‑137 (MQ=255) aGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGAt > 1:202703/1‑139 (MQ=255) aaGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTcc > 7:58474/1‑139 (MQ=255) cgcAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGa > 7:97889/1‑138 (MQ=255) cAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGaaaa < 6:297112/139‑1 (MQ=255) cAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGATCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGaaaa < 2:21171/139‑1 (MQ=255) aGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCAGAAAAg < 2:38596/139‑1 (MQ=255) gATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGc < 8:97889/139‑1 (MQ=255) gATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGc < 4:223549/139‑1 (MQ=255) gATTCTCACGAACAACGGAGAGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGc < 2:120508/139‑1 (MQ=255) aCGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGATAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTa < 3:99542/137‑1 (MQ=255) cGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTa < 7:61664/139‑1 (MQ=255) acaacGGAGTGAAGGGCGGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCAGAAAAGAGAGTATTACAt > 8:24766/1‑139 (MQ=255) acaacGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATg < 6:209712/84‑1 (MQ=255) acaacGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATg > 5:209712/1‑84 (MQ=255) cGGAGTGAAGGGCCGCAATTACGAGCTCACGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGaa < 5:91390/139‑1 (MQ=255) cGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCg > 7:194247/1‑137 (MQ=255) cGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCg < 8:194247/137‑1 (MQ=255) ccGCAATTACGAGCTCAAGGACGGCATATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGca > 7:285821/1‑85 (MQ=255) ccGCAATTACGAGCTCAAGGACGGCATATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGca < 8:285821/85‑1 (MQ=255) acGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGTCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAAc > 8:254586/2‑138 (MQ=255) cGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAAtctctc < 8:141557/60‑1 (MQ=255) cGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAAtctctc > 7:141557/1‑60 (MQ=255) cGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAAtctc > 5:32837/1‑58 (MQ=255) cGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAAtctc < 6:32837/58‑1 (MQ=255) cGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAAcc > 8:258718/1‑138 (MQ=255) cGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAAcc > 1:136010/1‑138 (MQ=255) cAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAg > 2:167919/1‑139 (MQ=255) aTTACGAGCTCAAGGACGGCATATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa < 3:134247/70‑1 (MQ=255) aTTACGAGCTCAAGGACGGCATATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa > 4:134247/1‑70 (MQ=255) cGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTc > 2:48270/1‑139 (MQ=255) ggACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTCCAGCATCGc < 7:90836/139‑1 (MQ=255) ggagTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTCCAGCATCGCAAg < 5:281188/135‑1 (MQ=255) ggCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAATCCAAGCTCTTCCAGCATCGCAAg > 1:268966/1‑138 (MQ=255) gCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa < 1:170922/52‑1 (MQ=255) gCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGa > 2:170922/1‑52 (MQ=255) cGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTCCAGCATCGCAAg > 2:152603/1‑136 (MQ=255) cGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTCCAGCATCGCAAg < 1:152603/136‑1 (MQ=255) cGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTCCAGCATCGCAAGGa > 3:185148/1‑138 (MQ=255) cGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTCCAGCATCGCAAGGa < 4:185148/138‑1 (MQ=255) | AAATCAAGCGTCAAAACCGAAAAAGAGCTGAAAGAGGTGCTGTCCTTCCTCGATAAGATGAATGATAAGGAAGCGCAGATTCTCACGAACAACGGAGTGAAGGGCCGCAATTACGAGCTCAAGGACGGCGTATTCACCTCACTTGAAAAAAACAATAAATCTCTCCTGTATGAGCATGAAGGCTTGGCACAGTTCAGCATGTCGATTCCGAAAAGCGAGTATTACATCGAAGACCAGAAAACCAAGCTCTTCCAGCATCGCAAGGA > CP006881/743571‑743836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |