BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	131	G→C	6.0%	intergenic (–/‑2847)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	131	0	G	C	6.0%	11477.4 / 97.8	4274	intergenic (–/‑2847)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (2020/1642); new base C (8/298); total (2032/2242)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4284 total aligned reads displayed.

‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGC  >  pSIJ663_pHT315_araE_xy/1‑268

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                          <  3:183169‑M1/132‑1 (MQ=255)

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                          <  1:34754‑M1/132‑1 (MQ=255)

caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                          <  5:58831‑M1/132‑1 (MQ=255)

 aataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAAAATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGaaa                                                                                                                                        >  1:54296‑M1/6‑139 (MQ=255)

        cATCCTCCAACGTTGGCTAGTGAGTTTTATGTCGAAAATATTAATGTTGCTGGGGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 <  7:189252/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGTGTGTGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATATAAACATAGCGGTAAAATTAGCAGTAACTAAATACAACGGAAATCAAAAAAGCCa                                                                                                                                 <  2:98154/139‑1 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 >  6:320392/1‑139 (MQ=255)

        cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 >  6:259765/1‑139 (MQ=255)

         cagctccAATGTTGGTGAGTGAGTTTTATGTCGCAAATTTTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGGGGTAAATTTAGCAGTAACTTAATAGCACGGAAATGAAAAACGCCAc                                                                                                                                <  5:102290/136‑1 (MQ=255)

          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATTGCGGTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                               <  7:168892/139‑1 (MQ=255)

          tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                               >  4:276149/1‑139 (MQ=255)

           cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                              >  7:119111/1‑139 (MQ=255)

               cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATACAACGTAAATGAAAAAAGCCACTCTCat                                                                                                                          <  2:25980/139‑1 (MQ=255)

                aaaGTTGTTGAGTGATTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATATAAAAATAGCTGTAAAATAAGCATAAACTTAATATAACGGAAATGAAAAAAGCCACTCTCata                                                                                                                         <  7:297456/139‑1 (MQ=255)

                 aaGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCatat                                                                                                                        >  5:288357/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTact                                                                                                                  >  3:15748/1‑137 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  2:128715/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  2:301140/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  1:309025/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  4:159915/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAAAAACATAGCTGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  5:263965/1‑139 (MQ=255)

                       gagaGTGAGTTTTATGTCGCAAATATTAATGATTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  6:160573/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                             >  2:102538/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                             >  5:313112/1‑139 (MQ=255)

                            tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                             >  4:13529/1‑139 (MQ=255)

                              agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                            >  5:210794/1‑138 (MQ=255)

                               tttttATGTCTAAAATATTATTGTTTCTGGTGAACATTATAAAATTTTCGTTTATTTAATACAAAAATAGCGGAAAAATTAGCAGAAAATAAATACAACGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                           <  4:49024/137‑1 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAAGCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  7:278905/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  7:211299/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  3:68001/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  7:101052/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  8:191056/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  8:306889/1‑137 (MQ=255)

                                          cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACAAACCTTTAGc                                                                                                 >  1:145968/1‑137 (MQ=255)

                                             attttAATGTTTCTTGTTACCCTTATAAATTTTTCGTTTTTTTAATAGAAAAATAGCGGTAAATATAGAAGTAACTTAATAGAATGTAATTTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  6:289871/136‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  7:143986/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  4:254607/139‑1 (MQ=255)

                                             atatTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATATAATCATAGGGTTAAAATTACCAGTAAATTAATATAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  7:51853/139‑1 (MQ=255)

                                                   ttGTTTTTGTTGAACCTTATAAAATTTTCGTTTATTAAATTAAAAAATAGCGGTAAAATTAGCATTAACTTAATACAACGGAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  2:33972/137‑1 (MQ=255)

                                                   aTGTTTCTGGTTAACCTTAACAATTTTTGTTTTATTAAATAGAAACATCGCGGTAAAATTACCAGAAACTAAAAAGACCGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  3:300152/138‑1 (MQ=255)

                                                   aTGTTTCTGGTGAACCTAATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  7:252373/138‑1 (MQ=255)

                                                      tttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                    <  1:23688/138‑1 (MQ=255)

                                                      tttCTGGTGAACCTTATAAAATTTTCTTTTATTTAATAGAAAAATAGGGTTAAAATTAGCATTAAATTAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                    <  8:237211/138‑1 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGATAAAAGCCCCTCTCATATGCTATTGGCTACCAACCTTTCGCGAGAATGACTTAATcc                                                                                 >  4:184227/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                               >  5:135470/1‑93 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                 >  8:83535/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                 >  5:118665/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACATTTAGCGAGAATGACTTACTcc                                                                                 >  1:284763/1‑139 (MQ=255)

                                                        tCTGGTGAACCTTATCAAATTTACGTTGATATAAAAGAAACATAGCGGTAAAATTAGAAGAAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTCGCTACAAACCTATATCGAGAATGACTTAATca                                                                                 >  8:170152/1‑138 (MQ=255)

                                                         cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTACTCCt                                                                                >  5:34793/1‑139 (MQ=255)

                                                           ggTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTTATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                               >  7:35323/1‑138 (MQ=255)

                                                            gttaaCCTTATCAATTTTTCGTTTATTAAATACAAAAATACATTAAAAATTAGAAGTAAATTAATACAACGTAAATCAAAAAAGCCACTCTCATATGCTATAGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  4:113235/136‑1 (MQ=255)

                                                            gttaaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTAAAAATTAGCAGTAACTTAATACAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  4:298199/136‑1 (MQ=255)

                                                            gTGAACCTTATCAAATTTTCGTTTATTTAATATAAACATAGCTGTATAATTAGCAGAAACTTATTATAACGTAATTGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  8:109438/139‑1 (MQ=255)

                                                            gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  2:208966/139‑1 (MQ=255)

                                                            gTGAACATTATAAAATTTTCGTTGATTTAATAGAAAATTATCGTTAAAATTAGCTTAAAATTAATATAACGGAATTTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             <  1:78491/139‑1 (MQ=255)

                                                              gAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                             >  5:241971/1‑137 (MQ=255)

                                                               aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCTACCTTTAGAGAGAATGACTTAATACTGTACAg                                                                          >  5:179374/1‑139 (MQ=255)

                                                               aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  6:115023/139‑1 (MQ=255)

                                                               aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          >  4:319527/1‑139 (MQ=255)

                                                                acattATCAATTTTTCGTTTATTTATTAGAAAATTAGCGGTAAAATTAGAATTAACTTATTAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  6:229225/135‑1 (MQ=255)

                                                                aCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGc                                                                         >  8:55714/1‑139 (MQ=255)

                                                                 ccTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGc                                                                         >  6:69164/1‑138 (MQ=255)

                                                                 ccTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGc                                                                         >  3:205850/1‑138 (MQ=255)

                                                                    tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTCCAGCCATa                                                                     >  4:7974/1‑139 (MQ=255)

                                                                    tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                     >  3:48344/1‑139 (MQ=255)

                                                                           ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACt                                                              >  2:153811/1‑139 (MQ=255)

                                                                           ttttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACt                                                              >  4:209172/1‑139 (MQ=255)

                                                                                gTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGa                                                          <  8:215431/138‑1 (MQ=255)

                                                                                              aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGATgcgc                                            >  1:218791/1‑138 (MQ=255)

                                                                                              aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGgcgc                                            >  1:123191/1‑138 (MQ=255)

                                                                                               aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            >  8:32726/1‑89 (MQ=255)

                                                                                               aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                               >  3:230399/1‑102 (MQ=255)

                                                                                               aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                               <  4:230399/102‑1 (MQ=255)

                                                                                                   aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                      >  8:291042/1‑139 (MQ=255)

                                                                                                          aaaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                 >  7:90278/1‑137 (MQ=255)

                                                                                                           aaTTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                 >  5:98231/1‑136 (MQ=255)

                                                                                                               aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCACCCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                          >  1:171750/1‑139 (MQ=255)

                                                                                                                gCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAtt                         >  1:259604/1‑139 (MQ=255)

                                                                                                                ccAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                          <  3:275184/137‑1 (MQ=255)

                                                                                                                      aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                 <  3:254417/125‑1 (MQ=255)

                                                                                                                      aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                   >  6:137344/1‑139 (MQ=255)

                                                                                                                      aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGAGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                   >  8:263718/1‑139 (MQ=255)

                                                                                                                            tAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc             <  2:179994/139‑1 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGa                                                          >  5:15617/1‑89 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  1:100138/1‑139 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  3:138492/1‑139 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  3:119835/1‑139 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  5:74304/1‑139 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTAAAATAATTTATTTGCATTGTTTTcgcta         >  2:61780/1‑136 (MQ=255)

                                                                                                                                 cGGAAATGAAAAAAGCCACTATCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            >  1:191445/1‑55 (MQ=255)

                                                                                                                                 aggAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTAGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        <  6:187069/138‑1 (MQ=255)

                                                                                                                                  gtaaaTCATAAAAGGCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGAACAGCGATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc       <  3:322881/137‑1 (MQ=255)

                                                                                                                                  ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc       <  3:92254/139‑1 (MQ=255)

                                                                                                                                   gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCATCCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa      <  7:31643/139‑1 (MQ=255)

                                                                                                                                   gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa      <  2:233021/139‑1 (MQ=255)

                                                                                                                                   gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa      <  7:74279/139‑1 (MQ=255)

                                                                                                                                     aaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAg    <  8:138873/139‑1 (MQ=255)

                                                                                                                                     aaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAg    <  7:23125/139‑1 (MQ=255)

                                                                                                                                      aTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAgg   >  6:50322/1‑139 (MQ=255)

‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGC  >  pSIJ663_pHT315_araE_xy/1‑268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`