BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,729	T→G	7.4%	intergenic (+14/‑142)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,729	0	T	G	7.4%	8441.4 / 140.6	2871	intergenic (+14/‑142)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base T (236/2357); new base G (259/12); total (496/2375)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3039 total aligned reads displayed.

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3591‑3872

    tACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAGAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCGC‑ttttt                                                                                                                                               >  7:155727/1‑139 (MQ=255)

    tACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGTAGGAAATAATAATATGAGTCGC‑gtttt                                                                                                                                               >  5:191225/1‑139 (MQ=255)

    tACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttttt                                                                                                                                               >  4:100344/1‑139 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                       <  8:313530/139‑1 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                       <  2:264500/139‑1 (MQ=255)

              atttttCATAAATAAATGAAACAAGCCAAAGTAAACAATTTAAGTACAATTACTTATGAGCAAGTATTGTCTATTTTTAATTGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTgg                                                                                                                                      <  1:156502/135‑1 (MQ=255)

               aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgta                                                                                                                                     <  8:101233/138‑3 (MQ=255)

               aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                     <  1:128637/138‑1 (MQ=255)

                 tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGGCTATTTTTAATAGTTAGCTATTTTTTAACGGGAGGAAATAATTCTATGAGTCGCGTTGTTTAAATTTGGata                                                                                                                                   >  2:214934/1‑137 (MQ=255)

                    catcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                               <  2:150477/139‑1 (MQ=255)

                     atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                               <  4:191096/138‑1 (MQ=255)

                      tcaAGCAATTAAACACGCCAAAGTAAACATTTTAAGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTATAAATTTGGAAAGTTac‑a                                                                                                                            <  3:242782/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                            <  7:199353/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                            <  7:115095/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                            <  6:87212/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                            <  3:27031/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                            <  6:162436/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                            <  8:215215/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                            <  5:65451/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                            <  4:94568/139‑1 (MQ=255)

                        aaGCAATGAAACACTCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                          <  6:217244/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑ac                                                                                                                           <  1:10315/138‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                          <  2:85588/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                          <  7:241517/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                          <  4:319744/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                          <  7:330964/139‑1 (MQ=255)

                           cAATGAAACACGCCAAAGTAAACTATTTAAGTACCATTTCTTATGAGGAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTa                                                                                                                       <  3:202141/139‑1 (MQ=255)

                           cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTa                                                                                                                       <  2:61710/139‑1 (MQ=255)

                             aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACt                                                                                                                     <  1:252343/139‑1 (MQ=255)

                              tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTG‑ACGtttat                                                                                                                     >  6:293122/1‑135 (MQ=255)

                                 aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAg                                                                                                                 <  8:283620/139‑1 (MQ=255)

                                    acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGa                                                                                                              <  1:41932/139‑1 (MQ=255)

                                    acGCCAAAGAAAAAAATTTAAGTTCCATTACTTATGAGCAAGTATTGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGATTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGa                                                                                                              <  5:166446/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGaa                                                                                                             <  5:3999/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTCAC‑ACGTTACTAAAGGGaa                                                                                                             <  3:156277/139‑1 (MQ=255)

                                      gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTTTAAGTTTA‑ACGGTTCTATAGGGatt                                                                                                            >  6:63815/1‑137 (MQ=255)

                                           aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGgaga                                                                                                       <  3:310253/139‑1 (MQ=255)

                                            gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGAt                                                                                                      <  6:37831/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATa                                                                                                     <  3:3772/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATa                                                                                                     <  5:271413/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATa                                                                                                     <  4:219663/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  7:5388/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  8:54597/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  7:13807/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  6:236493/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  1:89158/139‑1 (MQ=255)

                                                     ttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  1:64725/138‑1 (MQ=255)

                                                      tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTTAATTTAC‑ACGTTAATAAAGGGTATGGTGATAAattattt                                                                                             >  8:240036/1‑137 (MQ=255)

                                                       aaGTACCATTACTTAAGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                            <  7:263363/138‑1 (MQ=255)

                                                        ggTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                          <  7:310468/138‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGa                                                                                           <  5:144501/138‑1 (MQ=255)

                                                         gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                         <  2:114722/139‑1 (MQ=255)

                                                         gTACCATTACTTATGACCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                         <  3:184/139‑1 (MQ=255)

                                                          tACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGatat                                                                                        <  8:33568/139‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                       <  1:300952/138‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                       <  6:110304/138‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAACTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGTGAATTTAGATAAATTATTAGAtata                                                                                       <  6:184785/138‑1 (MQ=255)

                                                               ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTCAACTTTATAAAAGGTAAT‑GAGATAAATTTTTTtatataaaaa                                                                                   >  2:242132/1‑135 (MQ=255)

                                                                  cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                <  1:320273/139‑1 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                               <  8:125256/137‑1 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                               <  3:219815/137‑1 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                               <  8:1713/137‑1 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑GTAAGTTTA‑ACGGGTGTTAATGGGATGGGGGTAAATTTTTTTATTTACTACTTACAGCt                                                                           >  1:23205/1‑139 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTT‑ACGTTTATAAAGGGTATGGTGATTTATTATTATATATAATTCTGTCAGcg                                                                           >  3:55536/1‑138 (MQ=255)

                                                                           aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                       <  6:267762/139‑1 (MQ=255)

                                                                           aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                       <  7:287318/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAACTAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                     <  4:287302/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                     <  6:139052/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                     <  7:75700/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                     <  5:254809/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                     <  8:78826/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                     <  1:205118/139‑1 (MQ=255)

                                                                             gTATTGTCTATTATTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                     <  7:216109/139‑1 (MQ=255)

                                                                                 tGTCTATTTTTAATAGTTATCTATTATTTTACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                  <  3:7724/138‑1 (MQ=255)

                                                                                     tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                             <  1:178286/139‑1 (MQ=255)

                                                                                        ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAATTTAC‑ACGTTACTAAAGGGAATGGTGATAAATTATTAGATATAATAATGACAGCGTACAAGAAGCTAAagag                                                          >  8:260613/1‑139 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAATGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                          <  5:118607/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                          <  2:281828/138‑1 (MQ=255)

                                                                                          ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAgg                                                         <  5:296798/138‑1 (MQ=255)

                                                                                                    tCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAgcgc                                               <  2:38820/138‑1 (MQ=255)

                                                                                                          attTAACGGGAGGAAAAAATTCTATGCGCCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATAATAAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACgg                                        <  2:311976/138‑1 (MQ=255)

                                                                                                           tttAACGGGAGGAAATAATTCTATGAGTCGG‑TTTTTTATATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTTTTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACggt                                       <  7:171982/138‑2 (MQ=255)

                                                                                                                cGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTTAATTTTGTAATTTAC‑ACGTTTCTAAAAGGAATTGAGATAAATTTTTATATATTATACAGACAGCGTCCAAGAAGCATAATAGGTGCCTAGCGCCTTACgggtattt                                  >  2:191024/1‑134 (MQ=255)

                                                                                                                   gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgg                              <  4:121834‑M1/139‑3 (MQ=255)

                                                                                                                        aataatTCTATGAGTCGC‑ATTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTTTTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCC‑TACGGGGAATTTGgggtaca                         <  8:189222‑M1/139‑8 (MQ=255)

                                                                                                                          taatTATATGAGTAGC‑GTTTTTAAATTTTGAACTATAC‑ACGTTACTAAAGGGAATGGAGATAAATTATAAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacatt                       <  6:32454‑M1/139‑10 (MQ=255)

                                                                                                                              tCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaa                    <  4:43796‑M1/138‑13 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  8:148980‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGTAGATAAATTATTATATATACTCCTGACAGATTCCAAGAAGCTAAAGAGGGCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  4:70644‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  3:111351‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  3:286171‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  1:278366‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  4:25354‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  5:23168‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGA‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  5:89458‑M1/139‑18 (MQ=255)

                                                                                                                                   gAGTCTA‑TTTTTTTAATTTGGAAATTTAC‑AAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCAAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagg               <  3:134626‑M1/138‑18 (MQ=255)

                                                                                                                                    agtatc‑tttttTAAATTTTGATATTTAC‑TCTTTACTAAAGGTAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagga              <  5:20958‑M1/133‑19 (MQ=255)

                                                                                                                                    aGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaaggaa             <  4:171991‑M1/139‑20 (MQ=255)

                                                                                                                                    aGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaagga              <  4:148175‑M1/138‑19 (MQ=255)

                                                                                                                                     gTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGgggtacattgaaaaaggaag            <  5:321514‑M1/139‑21 (MQ=255)

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCC‑TACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3591‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`