BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,735	A→T	10.0%	intergenic (+20/‑136)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,735	0	A	T	10.0%	8535.7 / 287.5	3050	intergenic (+20/‑136)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (172/2541); new base T (322/14); total (494/2556)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3106 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGA  >  pSIJ663_pHT315_araE_xy/3597‑3874

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTATAtttt                                                                                                                                        >  6:56355/1‑138 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                       <  7:68365/139‑1 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                       <  4:228915/139‑1 (MQ=255)

        atttttCATAAATAAATGAAACAAGCCAAAGTAAACAATTTAAGTACAATTACTTATGAGCAAGTATTGTCTATTTTTAATTGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTgg                                                                                                                                       <  1:156502/135‑1 (MQ=255)

         aGTTTCATCAAGCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                      <  2:3599/138‑1 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                <  6:116929/138‑1 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTGTTGTAATTTTGTAAGt                                                                                                                                  >  5:71928/1‑136 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  8:328486/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  6:336618/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  8:144749/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  4:124581/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  5:177793/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  7:93417/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac‑ac                                                                                                                            <  1:10315/138‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                           <  5:204150/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                           <  5:284286/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                           <  7:252878/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                           <  4:110161/139‑1 (MQ=255)

                          aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTaaa                                                                                                                   <  2:209873/139‑1 (MQ=255)

                          aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTaa                                                                                                                    <  5:132184/138‑1 (MQ=255)

                              gcGCCAAATTAAACAATTTAAGTAACATTAATTAAGATGAAGTTTTTTCTAATTTTTATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGa                                                                                                               <  5:11205/138‑1 (MQ=255)

                              acGCCAAAGTAAACAATTTTAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGa                                                                                                               <  4:331484/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGaa                                                                                                              <  5:93729/139‑1 (MQ=255)

                                 ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATg                                                                                                            <  2:307729/139‑1 (MQ=255)

                                 ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATg                                                                                                            <  4:200203/139‑1 (MQ=255)

                                    aaGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGgag                                                                                                         <  1:84747/139‑1 (MQ=255)

                                     aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGgaga                                                                                                        <  8:112642/139‑1 (MQ=255)

                                      gTAAAAAATTTAAGTAAAATTAATTATTATGAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGTGAGGAAATAATTATATGAGTCGA‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGAt                                                                                                       <  6:228293/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATa                                                                                                      <  7:322250/139‑1 (MQ=255)

                                        aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATa                                                                                                      <  4:6061/138‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATaaa                                                                                                    <  8:306575/139‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATa                                                                                                      <  4:178959/137‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  1:315811/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  3:1539/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  7:233985/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  6:315756/139‑1 (MQ=255)

                                                tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTGTAATTTAA‑ACGGTACGAAAAGGAATTGTGTAAAattatttg                                                                                             >  2:109230/1‑137 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                             <  2:23939/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                             <  3:4502/138‑1 (MQ=255)

                                                  acagccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                           <  3:85723/135‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                           <  8:526/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                          <  3:107737/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                          <  1:273868/139‑1 (MQ=255)

                                                      gcATTACTTATGAGCTAGTATTGTCTATTTTTAATAGTTATCTATTAATTAACGGGAGGAAATAATTGTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                        <  3:296487/137‑1 (MQ=255)

                                                      ccATTAGTTATGAGCAAGTATTGTCTTTTTTTAATAGTTAGGTATTATTTAACGGGAGGAATTAATTGTATGAGTCGC‑TGTTTTTAATTTGGTTATTTAC‑AGGGTACTAAAGGGAATGGAGCTTAATTATTAGgtaga                                                                                        >  8:275804/1‑136 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                        <  1:176698/138‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                 <  2:82125/139‑1 (MQ=255)

                                                               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                <  6:7819/137‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  2:14240/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  7:168690/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  1:200453/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  7:107407/139‑1 (MQ=255)

                                                                      aGTATTGTCTAATTTTACTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTAGGAAAGTTAC‑ACGATACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                       <  6:28178/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑GAAAGTTACAACGTTA‑TAAAGTGTATTGAGATAAAATATTATATATACTAATGACAACTTcc                                                                         >  6:167090/1‑136 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAATTTTA‑ACGTTTCTAAATGGAATTGAGTTTAATTTATTTATATAATAATGAAAGCttac                                                                         >  1:24600/1‑134 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAAGTTTA‑CCGTTTATTAATGGTATTGTGATAAATTTTTTTATATTATACTGACAGCttgc                                                                         >  1:22333/1‑134 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAATTTAC‑ACGTTAATAAAGGGAATTGATATTAATTATTAGATATAATACTGACAGCTTcc                                                                         >  6:148627/1‑136 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                      <  2:59954/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                      <  1:299803/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                      <  1:36172/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGTGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATATGGAAAGTTAC‑AAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                      <  7:47634/139‑1 (MQ=255)

                                                                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                     <  3:196403/139‑1 (MQ=255)

                                                                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                     <  3:179803/138‑1 (MQ=255)

                                                                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAAATTTTTAAATTAC‑AAGTTACAAAATGGAAAGGAGTTTAATTATTTTATATAATACTGACATCTAGCAAAAAGcg                                                                 >  7:21162/1‑137 (MQ=255)

                                                                                aTTTTTTATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTCGA‑TTTTTTAAATTATGAAAGTTAC‑ACGTTACTAAAGTGAATGGAGATAATTTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                              <  5:243977/138‑1 (MQ=255)

                                                                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                             <  6:251581/138‑1 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTTATTTTTTTATTTTA‑AAGTTAATTAATGGGATTTATATAAATTATTATATATTATAATGACACCATACAAAAAGCTAAatag                                                           >  4:153998/1‑139 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTA‑ACGTTAATAAATGGAATGGTGTTAAATTATTTTATATTCTACGGTCACGTTCCAATAAGCGAAagag                                                           >  8:1855/1‑139 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTCACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                           <  8:165458/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                           <  7:149595/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                           <  1:56759/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                           <  3:236488/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                           <  7:278451/138‑1 (MQ=255)

                                                                                          gTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCTA‑TTTTTTAAATTTGTAAAGTTAC‑ACGTTACTAAAGTGAATTGAGATAAATTATTAGATATACTACTGACTGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                   <  4:26929/139‑1 (MQ=255)

                                                                                                  ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTGCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACg                                           <  2:180993/139‑1 (MQ=255)

                                                                                                     tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                         <  1:129797/138‑1 (MQ=255)

                                                                                                       tAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                       <  7:301177/138‑1 (MQ=255)

                                                                                                          cGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAAATTGGTAATTTTA‑AAGTTAAAAAAAGGAATGTTTTTTAATTATTATATATACTAATTACAGCATCCAAGAAGCAAAATAAGTCCCAAGCCGCAACGGGGAAttt                                   >  8:235074/1‑139 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  6:197684‑M1/139‑8 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  4:298521‑M1/139‑8 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  4:81578‑M1/139‑8 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  8:76325‑M1/139‑8 (MQ=255)

                                                                                                                      atTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                       <  8:256697‑M1/139‑12 (MQ=255)

                                                                                                                            tGATTCGA‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  5:220319‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGTGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  5:306180‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  3:133568‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  3:324073‑M1/139‑18 (MQ=255)

                                                                                                                             gAGTCTC‑TTTTTTAAATTTGGAACGTTAC‑ACGTTACTAAAGGTAATGGAGATAATTTATTAGTTATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  3:2624‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  3:68385‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  7:127867‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTAACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  3:21680‑M1/138‑18 (MQ=255)

                                                                                                                               gTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa               <  5:263753‑M1/138‑20 (MQ=255)

                                                                                                                                     ttttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  5:49332‑M1/139‑26 (MQ=255)

                                                                                                                                     ttttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  5:328920‑M1/139‑26 (MQ=255)

                                                                                                                                     ttttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  2:259838‑M1/139‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAATGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  5:155496‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  3:277028‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  7:187780‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTAC‑AAGTTACTAAAGGGAATGGAGATAAATTATAAGATATACTTCTGACAGCTTCCAAGAAGGTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtag         <  2:279304‑M1/137‑26 (MQ=255)

                                                                                                                                           aaaTTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTAATAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta    <  8:212213‑M1/138‑31 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGA  >  pSIJ663_pHT315_araE_xy/3597‑3874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`