BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,743	A→T	5.7%	intergenic (+28/‑128)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,743	0	A	T	5.7%	9338.5 / 80.6	2980	intergenic (+28/‑128)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (233/2527); new base T (193/13); total (439/2541)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3065 total aligned reads displayed.

CAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  pSIJ663_pHT315_araE_xy/3605‑3877

aaGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGa                                                                                                                                        <  3:291575/138‑1 (MQ=255)

 tttttAATCAAGAAATGAAAAACGCCAAAGTAAACAATTAAAGTACAATTACTTATGAGCAAGTATTGTCTTTTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGa                                                                                                                                        <  6:51720/136‑1 (MQ=255)

       atcaAGCAAGGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTa                                                                                                                                  <  8:302552/138‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACATTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTTACGGGAGGAAATATTTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTaga                                                                                                                                <  3:147683/139‑3 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTaca                                                                                                                                <  7:249470/139‑1 (MQ=255)

        acaggCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTaca                                                                                                                                <  1:171593/135‑1 (MQ=255)

          caccAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACg                                                                                                                              <  7:121988/136‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTacac                                                                                                                               <  1:10315/138‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTacac                                                                                                                               <  7:269618/138‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACg                                                                                                                              <  8:163818/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACg                                                                                                                              <  2:26845/139‑1 (MQ=255)

           aGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTTA‑TTTGTGAAGtttgccgg                                                                                                                             >  4:326150/1‑133 (MQ=255)

             cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGATGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTa                                                                                                                           <  6:18636/139‑1 (MQ=255)

               aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACt                                                                                                                         <  3:65161/139‑1 (MQ=255)

                  aaagaagCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTaa                                                                                                                       <  2:299793/132‑1 (MQ=255)

                  aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTTAA‑TTTTGAAAATTAAACGTtaataa                                                                                                                       >  8:32784/1‑138 (MQ=255)

                  aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTaa                                                                                                                       <  2:77972/138‑1 (MQ=255)

                   aaCACGCCAAAGTAAACAATTTAAGTACGATTACTTATGAGCATGTTTTGTCTATTTTTAATAGTTATCTATTATTTAAGGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTaaa                                                                                                                      <  6:74869/138‑1 (MQ=255)

                      acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATTATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                  <  4:192944/139‑1 (MQ=255)

                      acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                  <  4:56195/139‑1 (MQ=255)

                      acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                  <  7:317206/139‑1 (MQ=255)

                       cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                 <  4:122157/139‑1 (MQ=255)

                       cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                 <  2:327513/139‑1 (MQ=255)

                       cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                 <  8:50580/139‑1 (MQ=255)

                             aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                           <  4:79367/139‑1 (MQ=255)

                             aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                           <  7:64612/139‑1 (MQ=255)

                             aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                           <  2:124886/139‑1 (MQ=255)

                              gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                          <  5:172704/139‑1 (MQ=255)

                              gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                          <  7:87666/139‑1 (MQ=255)

                               tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  1:213474/139‑1 (MQ=255)

                               tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  6:309288/139‑1 (MQ=255)

                                 aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                       <  6:223608/139‑1 (MQ=255)

                                    aaTTTAATTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAatta                                                                                                    <  7:228343/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  1:265654/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGATAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  8:273076/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:19881/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  8:110747/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  7:103456/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  6:244323/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  2:162018/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTAAT‑TTTGGTTAGTTAAACGGTTATAAATGGAATGGTGATAAattttt                                                                                                  >  6:160393/1‑139 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  1:239120/139‑1 (MQ=255)

                                         aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  5:37280/138‑1 (MQ=255)

                                          aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                              <  5:297895/139‑1 (MQ=255)

                                          aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                              <  3:193544/139‑1 (MQ=255)

                                          aGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                              <  8:142645/139‑1 (MQ=255)

                                             aacATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTACCGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGatat                                                                                            <  2:225736/136‑1 (MQ=255)

                                              ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTTAT‑TTTTGTAAATTAGACGGGTATAAATGGAATGGTGAGAAAATATTatatattg                                                                                          >  2:55675/1‑137 (MQ=255)

                                              ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                           <  5:257944/138‑1 (MQ=255)

                                                    cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                    <  5:85939/139‑1 (MQ=255)

                                                    cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                    <  7:206747/139‑1 (MQ=255)

                                                    cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑GTTTTTAA‑TTTGGTAAGTTTAACGTTTATAAAGGGAATGGAGATAAAATATTATTTATAAGACTGa                                                                                    >  3:130692/1‑139 (MQ=255)

                                                      tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTTAAT‑TTTGGAATTTTGC‑CGTTTATAAAGGGTATTGTGTTTAATTTTTATATATACTTCTGACa                                                                                  >  1:31144/1‑139 (MQ=255)

                                                         gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTTGAAAATTTAACGTTAATAAAAGGAATTGAGATAAAATATTATATATAAAAATGACAACt                                                                               >  7:4056/1‑139 (MQ=255)

                                                             aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  1:149017/139‑1 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTAAA‑ATTTGAAATATAAACCTTACAAAAAGGAATGGAGATTAATTATTATATATACAAATGACAGCATCCa                                                                           >  8:21215/1‑138 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTAGGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                          <  5:149209/139‑1 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGT‑TTTTTAAA‑TTT‑GTAATTTTCACGTTTGGAAAAGGTATTGTGATTAATTATTATATTTAATACTGAAAGCTTcc                                                                            >  5:63577/1‑136 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  3:232987/139‑1 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTAAATTTTGTAAAGTT‑CACGTTTATATATGGTAAGGAGTTAAATTATTATATTAACAAATGACAGAATCCaag                                                                         >  8:256081/1‑139 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGACGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  6:1054/139‑1 (MQ=255)

                                                                tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  5:214410/139‑1 (MQ=255)

                                                                 aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  3:103465/138‑1 (MQ=255)

                                                                    gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGc                                                                     <  4:293097/138‑1 (MQ=255)

                                                                     tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGATACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                   <  1:289843/139‑1 (MQ=255)

                                                                         tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                 <  3:40987/137‑1 (MQ=255)

                                                                          ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑G‑TTTTTTAA‑TTTTGTAAGTTTAACGTTAATAAATGGAATGGTGTTAAATTATTTTATATTCTACGGTCACGTTCCAATAAGCGAAagag                                                              >  8:1855/1‑139 (MQ=255)

                                                                           tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  1:74527/138‑1 (MQ=255)

                                                                           tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  3:212728/138‑1 (MQ=255)

                                                                           tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  1:189436/138‑1 (MQ=255)

                                                                                          ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTTAA‑TTTTGAAAGTTTAACGTTAATAAATGGAATGGAGATAAATTATTATATATAATACTGACAGCTTACAAGAAGCTAAATAGGTCCCTAGCGCCTACg                                              >  3:257370/1‑139 (MQ=255)

                                                                                          ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCt                                                 <  1:50883/136‑1 (MQ=255)

                                                                                             tttAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTTAA‑TTTGGAAAGTTACACGTTTATAAAAGGAATTGAGATTAATTATTATATATAATACTGACAGCATACAATAAGCTAAAGAGGTACCTAGCGCCGACgggg                                           >  5:123515/1‑139 (MQ=255)

                                                                                                aaCGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                        <  4:218463/139‑1 (MQ=255)

                                                                                                 aCGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                        <  4:150303/138‑1 (MQ=255)

                                                                                                          aataatTCTATTAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGTGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCAAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  3:171199‑M1/139‑8 (MQ=255)

                                                                                                          aataatTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  8:76325‑M1/139‑8 (MQ=255)

                                                                                                               ttCTATGAGTCGC‑G‑TTTTTAAA‑TTTTGAAAGTTAAACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTAATGACAGCGTGCAAGAATAGAAAAAAGTACCTAGCGCCTACGGGGAATTTTgggtacattta                          >  7:182326‑M1/1‑127 (MQ=255)

                                                                                                                    tGAGTCGC‑T‑TTTTTAAA‑TTTTGAAAGTTAAACGTTACTAAATGGTATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGCATTTGgggtaaattgaaaaag                     >  7:163954‑M1/1‑122 (MQ=255)

                                                                                                                    tGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTATAGGGCATGGAGATAAATTATTAGATATACTACTGACCGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  2:248136‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:232980‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  4:169520‑M1/139‑18 (MQ=255)

                                                                                                                    tGACTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  6:332808‑M1/139‑18 (MQ=255)

                                                                                                                     gAGTCTC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGATTGGATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  6:273478‑M1/138‑18 (MQ=255)

                                                                                                                     gAGTCGA‑T‑TTTTTATA‑TTTTGAAATTTTCACGTTACTAAAGGGAATGTAGATAAATTATTATATATACTACTGTCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgtgtacattgaaaaagg                    <  3:276058‑M1/138‑18 (MQ=255)

                                                                                                                       gTCTC‑T‑TTTTTAAA‑TTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACATCTTCCAATAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:292174‑M1/138‑20 (MQ=255)

                                                                                                                       gTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                 <  5:321514‑M1/139‑21 (MQ=255)

                                                                                                                        tCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                 <  5:273312‑M1/138‑21 (MQ=255)

                                                                                                                               tttttAAA‑TTTGGAAATTTACACGTTAATAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  3:79050‑M1/138‑26 (MQ=255)

                                                                                                                                ttttTAA‑TTTTGTAAGTTACACGTTACTAAAGTGTATGGAGATAATTTATTAGATATACTACTGACAGCTTCAAAGAAGCTAAAGAGGTCACTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  1:328762‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAA‑TTTTGAATGTTACACTTTACTAAAGGTAATTGAGAAAAATTTTTAGATATACTACTGACAGCTGCGAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGTATTTGgggtacattgaaaaaggaagagtat            <  7:147258‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  3:98136‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCGTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:105348‑M1/137‑26 (MQ=255)

                                                                                                                                ttttAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATAAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  2:197973‑M1/137‑26 (MQ=255)

                                                                                                                                    aaa‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  6:134567‑M1/138‑31 (MQ=255)

                                                                                                                                     aa‑TTTGTAAAGTTACACTTTACTAAAGGGAATGTTCATAATTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  3:318511‑M1/139‑33 (MQ=255)

                                                                                                                                     aa‑TTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  8:236359‑M1/139‑33 (MQ=255)

                                                                                                                                     aa‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCACGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  4:256640‑M1/139‑33 (MQ=255)

                                                                                                                                     aa‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt     <  2:100696‑M1/139‑33 (MQ=255)

                                                                                                                                          tGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaa  <  1:126308‑M1/138‑36 (MQ=255)

CAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑T‑TTTTTAAA‑TTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  pSIJ663_pHT315_araE_xy/3605‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`