BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,749	A→T	5.5%	intergenic (+34/‑122)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,749	0	A	T	5.5%	8461.1 / 66.6	2698	intergenic (+34/‑122)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (243/2261); new base T (179/8); total (426/2272)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2988 total aligned reads displayed.

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGA  >  pSIJ663_pHT315_araE_xy/3611‑3874

 atcaAGCAAGGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTa                                                                                                                                <  8:302552/138‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  2:291333/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  5:111193/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  4:121455/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  1:189299/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  6:162436/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  6:309590/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑a                                                                                                                             <  4:81490/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑ac                                                                                                                            <  7:40941/138‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTac‑ac                                                                                                                            <  1:10315/138‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                           <  7:122922/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACg                                                                                                                           <  8:163818/139‑1 (MQ=255)

     aGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGt                                                                                                                          <  7:250257/139‑1 (MQ=255)

      gCAATGAAACACGCCATAGTTAACAATTTATGTAGGATTTCGTTTTATCTAGTTTTGTCTATTTTTAATTGTTATCTATTATTTAAGGGGATGATTTATTTATATGAGTTGTTTTTTTAAATTTGGAAAGTTAC‑AAGtt                                                                                                                         <  7:105065/139‑1 (MQ=255)

         ttcaaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACt                                                                                                                      <  1:169332/136‑1 (MQ=255)

         aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACt                                                                                                                      <  2:121969/139‑1 (MQ=255)

         aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTGGAAAGTTAC‑ACGTTACt                                                                                                                      <  8:184633/139‑1 (MQ=255)

            aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTAAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTaaa                                                                                                                   <  5:217071/139‑1 (MQ=255)

            aaaCACGACAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTaaa                                                                                                                   <  1:327038/139‑1 (MQ=255)

                acGCCAAAGTAAACAATTTTAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGa                                                                                                               <  4:331484/139‑1 (MQ=255)

                acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATTATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGa                                                                                                               <  4:192944/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGaa                                                                                                              <  5:152268/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTAAGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGaa                                                                                                              <  3:290682/139‑1 (MQ=255)

                        gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGAt                                                                                                       <  5:235637/139‑1 (MQ=255)

                        gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGAt                                                                                                       <  3:77305/139‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATa                                                                                                      <  4:6061/138‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATa                                                                                                      <  2:19151/138‑1 (MQ=255)

                           aaCAATTTAACTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATaaa                                                                                                    <  2:151955/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTTTATTTGTAAGTTTA‑ACTTTAATAAATGGAAATGATATAAattatt                                                                                               >  4:148150/1‑139 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  3:145148/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  1:210340/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  3:97132/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  6:244323/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  3:101527/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  8:110747/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAattatt                                                                                               <  6:106353/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAGTTTC‑ACGGTTATAAAGGGAATTGAGATAAattatt                                                                                               >  2:54456/1‑139 (MQ=255)

                                  tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAAttatta                                                                                              <  7:88904/138‑1 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                             <  8:254209/138‑1 (MQ=255)

                                    aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                           <  3:314399/139‑1 (MQ=255)

                                    aGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                           <  8:142645/139‑1 (MQ=255)

                                     gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCTCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGTAATGGAGATAAATTATTAGata                                                                                          <  5:277383/139‑1 (MQ=255)

                                       aCCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                        <  2:321161/139‑1 (MQ=255)

                                        ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                        <  1:147948/138‑1 (MQ=255)

                                        ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                        <  3:192480/138‑1 (MQ=255)

                                        aaaTTACTTATTAGCAAGTATTGTCTATTTTAAATAGTTATCTATTATTTAACGGGAGGAAATAATTCAATGAGTCGCTTTATTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                        <  6:187263/136‑1 (MQ=255)

                                           ttACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAAGTTTCAACTTTATAAAAGGTAAT‑GAGATAAATTTTTTtatataaaaa                                                                                    >  2:242132/1‑135 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                 <  7:147344/139‑1 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                 <  6:76633/139‑1 (MQ=255)

                                               ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                <  7:182601/139‑1 (MQ=255)

                                                  tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAg                                                                              <  2:265510/138‑1 (MQ=255)

                                                    aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTTGAAATTTAA‑ACGTTAATAAATGGAATTGATATAAATTATTATATATAATAATGAAAGCtt                                                                           >  6:224422/1‑139 (MQ=255)

                                                    aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTTTTAGTTAA‑ACGTTACTTAAGTGATTGGTGATTAATTTTTTGATATAATAATGAAAGcg                                                                            >  3:61658/1‑137 (MQ=255)

                                                      cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTGGAAAGTTTA‑ACCTTTCGAAATGGTTTTGTGATAAAATTTTTTTTATTATACTGGCAGCTTc                                                                          >  3:127182/1‑138 (MQ=255)

                                                       aattATTTTCTCTTTTTAATATTTATCTATTATGTAACGTGAGGAAATCATTCTCTTAGTCTCTTTTTTAAATTTGTAAATTTAC‑ACGTTAATAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  4:26006/136‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  3:39534/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  5:175303/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGATATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                        <  3:275699/139‑1 (MQ=255)

                                                        aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAAATTTGTAAGTTTA‑ACGGTACTAAATGGAATTGAGATAAATTTTTATATATAATAAAGACAGCTTACa                                                                        >  6:137120/1‑138 (MQ=255)

                                                        aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAAATTTGAAATATAA‑ACCTTACAAAAAGGAATGGAGATTAATTATTATATATACAAATGACAGCATCCa                                                                        >  8:21215/1‑138 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTAGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                      <  8:327775/139‑1 (MQ=255)

                                                          tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                     <  1:70795/139‑1 (MQ=255)

                                                          tATTGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                     <  5:323350/139‑1 (MQ=255)

                                                           aTTGTCTATTTTTAATATTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                     <  5:199914/138‑1 (MQ=255)

                                                           aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                     <  7:292367/138‑1 (MQ=255)

                                                                 tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTAATTGGTAAATTAG‑ACGTTTGTAAATGGATTGTAGATAAATTAATATATATAATTCTGACAGCTTCGAACGAGCTaaa                                                              >  8:235300/1‑139 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAACTTCCAAGAAGCTAAagag                                                           <  1:309574/138‑1 (MQ=255)

                                                                            tttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                   <  2:212954/138‑1 (MQ=255)

                                                                                     tttttAACGGTAGTAAATAATTCTTTGAGGCGCTTTTTAAAATTTGTAAATTTAC‑ACGTTAATAACGGTAATGTATAT‑AATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                          <  3:212153/136‑1 (MQ=255)

                                                                                      attTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                          <  5:66155/138‑1 (MQ=255)

                                                                                       tttAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACCGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                         <  7:318268/138‑1 (MQ=255)

                                                                                       tttAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTTATTTTGTTAGTTAA‑AAGTTAAGAAATGGTATGGAGATAAATTATTTTATATAATACTTAAAGCTTCCAACAACCGAAAAAGGGGCCGAACGCCAGCgggg                                        >  4:171574/1‑139 (MQ=255)

                                                                                          aaCGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                     <  4:218463/139‑1 (MQ=255)

                                                                                           aCGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                     <  7:65921/138‑1 (MQ=255)

                                                                                                    aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  1:261333‑M1/139‑8 (MQ=255)

                                                                                                    aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  3:168305‑M1/139‑8 (MQ=255)

                                                                                                    aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  3:232661‑M1/139‑8 (MQ=255)

                                                                                                    aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  3:255965‑M1/139‑8 (MQ=255)

                                                                                                    aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  8:76325‑M1/139‑8 (MQ=255)

                                                                                                    aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAG‑TAC‑ACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                           <  7:270087‑M1/138‑8 (MQ=255)

                                                                                                              tGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  7:208244‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  8:49908‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  5:38774‑M1/139‑18 (MQ=255)

                                                                                                              tGACTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  6:332808‑M1/139‑18 (MQ=255)

                                                                                                               gAGTCTCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGATTGGATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  6:273478‑M1/138‑18 (MQ=255)

                                                                                                               gAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACTTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  7:228660‑M1/138‑18 (MQ=255)

                                                                                                               gAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                 <  7:163965‑M1/138‑18 (MQ=255)

                                                                                                                aGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa               <  5:260177‑M1/139‑20 (MQ=255)

                                                                                                                aGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCGTACGGGGAATTTGgggtacattgaaaaaggaa               <  1:93901‑M1/139‑20 (MQ=255)

                                                                                                                aGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa               <  1:28634‑M1/139‑20 (MQ=255)

                                                                                                                aGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa               <  4:293672‑M1/139‑20 (MQ=255)

                                                                                                                      ttttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  5:131027‑M1/139‑26 (MQ=255)

                                                                                                                        ttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  2:7290‑M1/137‑26 (MQ=255)

                                                                                                                        ttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  1:4668‑M1/137‑26 (MQ=255)

                                                                                                                        ttttAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  1:238606‑M1/137‑26 (MQ=255)

                                                                                                                        ttttAAATTTGGAAAGTAAC‑ACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat         <  5:30024‑M1/137‑26 (MQ=255)

                                                                                                                            aaaTTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta    <  8:272192‑M1/138‑31 (MQ=255)

                                                                                                                             aaTTTGGAAAGTTAC‑CAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt  <  6:5424‑M1/139‑33 (MQ=255)

                                                                                                                             aaTTTGGAAAGTTAC‑ACGTTACTAATGGGTATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt  <  1:125695‑M1/139‑33 (MQ=255)

                                                                                                                              aTTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGTCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt  <  5:87200‑M1/138‑33 (MQ=255)

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC‑ACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGA  >  pSIJ663_pHT315_araE_xy/3611‑3874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`