BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	6,697	T→A	6.0%	intergenic (+1966/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	6,697	0	T	A	6.0%	6579.9 / 68.2	2366	intergenic (+1966/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (1296/885); new base A (6/168); total (1311/1055)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2520 total aligned reads displayed.

AAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATA  >  pSIJ663_pHT315_araE_xy/6559‑6834

 aacaaaaCCCCCTTCGGCTGGGGATTATTGTGTGGCGTACCCTCGCCGTAAAGATACGGCATAAACCAAACTGGCTCGGCTCTTTCATCCGCCTGTGGCGGTGCAGCTATTAAAGCTGGGACATTGCTCAGGCCGTTTa                                                                                                                                           <  6:76855/136‑1 (MQ=255)

  aGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTaa                                                                                                                                          >  5:55811/1‑139 (MQ=255)

       aCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑cg                                                                                                                                    <  2:269910/139‑1 (MQ=255)

           ccTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGc                                                                                                                                <  6:127371/139‑1 (MQ=255)

           ccTTCGCCTGGGGATTATTGAGAGGCGTACGCTCGCCCGAAAGATAAGCCAGAAACCAAACTTGCTCGCCACTTTCATCAGCCAGATGAGCTCCAGCGATTAAAGCTCGGACATTGCTCAGGCCGGTTAATTT‑CGCGGc                                                                                                                                >  5:259512/1‑139 (MQ=255)

            cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGcc                                                                                                                               <  3:145237/139‑1 (MQ=255)

            cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGcc                                                                                                                               >  4:50654/1‑139 (MQ=255)

            cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGcc                                                                                                                               <  2:231895/139‑1 (MQ=255)

            cTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGcc                                                                                                                               <  5:131970/139‑1 (MQ=255)

            cTTCGACTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACGGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGcc                                                                                                                               <  2:207797/139‑1 (MQ=255)

              tCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGccc                                                                                                                              >  8:78549/1‑138 (MQ=255)

                gCCTGGGTATAATTGTGTGGCGTACGCTCGCAGGAAAGATAAGGCACAAACCAACCTGGCTCGGAACTTTCATCAGCCTGTTGAGCTGCAGCGATAAAAGCTGGGACATTGCTCAGGCCGGTTATTTT‑CGCGGCCCAAt                                                                                                                           <  8:103915/139‑1 (MQ=255)

                     gggATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGa                                                                                                                      >  1:8712/1‑139 (MQ=255)

                       gATTATTGTGTGGCGTAGGCTCGCCGTATAGAAAAGGAAGAAACCACAGTGGCTCGGAACTTTAATCCGCCTGTTTAGCTGCAGAGATTAACGCTGGGACATTGCTCAGGCCGGTTAATTT‑GGCGGCCCAATCCaccca                                                                                                                    <  8:211574/139‑5 (MQ=255)

                       gATTATTGTGGGGCGTCCGCTCGCCGTAAAGATCAGCAACAAACCAAACTGGCGCGGCACTTTCATCAGCCTGTTGAGCTGCCGCTATAAACGCTGGTACATTGCTCCGGCCGGTAAATTT‑CGCGGCCCAATCCAGaca                                                                                                                    <  8:19919/139‑1 (MQ=255)

                          tattGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACacga                                                                                                                 >  4:294282/1‑139 (MQ=255)

                               tggggCGAACGCCCTCCGGAAATAAACGCCAGAACCCAAACTGCCGCGGCACTTTCATCAGCCGGTGGCGCTGCCGCTATAAAAGCTGGGACTGTGCGCAGGCCGGTTAATTT‑GGCGGCCAAAGCCCCACACGACGCt                                                                                                             <  6:167653/135‑1 (MQ=255)

                                gtgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGCCATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAGTCCAGACACGACGCTGc                                                                                                           >  5:32963/1‑139 (MQ=255)

                                 tgGCGTACGCTCGCCGGAAAGATAAGGCAGAACCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGCCACGACGCTGCa                                                                                                          >  3:5179/1‑139 (MQ=255)

                                 tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACacgacctgtct                                                                                                          >  3:123453/1‑133 (MQ=255)

                                 tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                                          >  6:100814/1‑139 (MQ=255)

                                 tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                                          >  6:191370/1‑139 (MQ=255)

                                 tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                                          >  8:301431/1‑139 (MQ=255)

                                 tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                                          >  3:34005/1‑139 (MQ=255)

                                 tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                                          >  1:120481/1‑139 (MQ=255)

                                 tgGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                                          >  1:235507/1‑139 (MQ=255)

                                 tgGCGTACGCCGGCCGTAAAGATCCGGCATAAACCAACCTGGCGCGGCACTTTCCTCCCCCGGTTGCGCTGCCGCTATAAAAGCTGGGACTTTGCTCCGGCCGTTTAATTT‑CGCGGCCAAATCCAGACACGACGCTGCa                                                                                                          <  8:132198/139‑1 (MQ=255)

                                 gggCGTACGCGCGCCGTAAATATACGGCAGAAACAAAACTGGCGCGGCACTTTCATCAGCCTGTTGAGCTGCAGCTATTAACGCGGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCa                                                                                                          <  8:62638/138‑1 (MQ=255)

                                   gggtccgcgcgCCGTAAAGATACCGCATAAACAAACCTGGCTCGGCACTTTCAGCCCCGTTTTGTGCTGCCCCGATTAAAGCTGGGACATTGCTCTGGCCGGTTATTTG‑CGCGGCCCAATCCAGACACGACGCTGCACt                                                                                                        <  8:25989/134‑1 (MQ=255)

                                      tACGCTCGCCGGAAAGATAAGGCAGAAACCAACCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                     >  4:320891/1‑139 (MQ=255)

                                      tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCCGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGCCACGACGCTGCACTCAg                                                                                                     >  1:217527/1‑139 (MQ=255)

                                      tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                     >  1:42188/1‑139 (MQ=255)

                                      tACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                     >  3:281138/1‑139 (MQ=255)

                                      tACGCTCGCCGGAAAGATAAGGCAGAAACAAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAg                                                                                                     >  2:6910/1‑139 (MQ=255)

                                      accgcgcgCCGGAACTATACGGCAGAAACAAAACGGGCTCGGCACTTTCATCAGCCGGTTGAGCTCCAGCTAATAAAGCGGGTAAATTGCTCCGGCCGTTTAATTT‑CGCGGCCCAAACCACACACGACGCTGCACTCAg                                                                                                     <  6:238729/137‑1 (MQ=255)

                                             gcgtcaaaTATAAGCCACAAACAAAAGTGCCGCGGAACTTTCCTCAGCCGTTTGCGCGGCTGCTATAAAAGCGGGTACATTCCTCCGCCGGTTTAATTC‑CGCGCCCAAATCCAGACACGACGCTGCACTCAGCATcaca                                                                                              <  2:31563/134‑1 (MQ=255)

                                             gCCGTAAACATAAGCAACAAACCAAACTGGCTGGAAACTTTCATCAGCCTGTTGAGCTGCAGCGATTAACGCGGGGCAATTGCTCAGGCCGGTTAATTG‑CGCGCCCCAATCCACACACGACGCTGCACTCAGCATcaca                                                                                              <  7:269994/139‑1 (MQ=255)

                                             gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATcaca                                                                                              <  7:195920/139‑1 (MQ=255)

                                             gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATca                                                                                                >  5:286040/1‑137 (MQ=255)

                                             gCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCGGGGACATTGCTCCGGCCGGTAAATTG‑CGCGGCCAAATCCAGACACGACGCTGCACTCAGCATcaca                                                                                              <  2:185853/139‑1 (MQ=255)

                                              ccttaaaTATAAGGCATAAACAAAACTGGCGCGGAAATTTCATAAGCTTTTTGAGCTGAAGCTATTAAAGCTGGTAAATTGCTCTGGCCGTTTAATTT‑CGCGGCCAAATCAAGACACGACGCTGCACTCAGCATCACAg                                                                                             <  8:160908/135‑1 (MQ=255)

                                              ccGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGcc                                                                                                                               <  1:28375/105‑1 (MQ=255)

                                                       tAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAAt                                                                                    >  3:227286/1‑139 (MQ=255)

                                                         aGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGGTATTAAATCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCGAGACACGAAGCTGCTCTCAGCATCTCATACTTTAAATGc                                                                                  >  6:216511/1‑139 (MQ=255)

                                                         aGGCACAAACCAACCTGGCTCGGCACTTTCAGCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGc                                                                                  <  3:299401/139‑1 (MQ=255)

                                                          gccAGAAACCAAAGTGGCTCGGCACTTTCATCCGCCGTTTGAGCTGCAGCTATTAAAGCTGGTACATTGCTCAGGCGGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                                                 <  4:201122/137‑1 (MQ=255)

                                                          gccAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCGGGGACATGGCGCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGcc                                                                                 <  7:35050/137‑1 (MQ=255)

                                                            cacaaaCCAAACGGGCGCGGCACTTTCATCCGCCTTTTGTGCGGCCGCGATTAACGCGGGGACTTGGCTCCGGCGGGTAATTTC‑CGCGCCCCAATCCCGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCaa                                                                               <  4:104241/136‑1 (MQ=255)

                                                            aaGAACCAAAAGTGGCTGGGCACTTTCTTCAGCTTGTTGAGCGGCAGCGTTAAAAGGTGGTACTTTGCTCCGGCGGGTTATTTG‑CGCGGCCCAAACCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCaa                                                                               <  4:271382/138‑1 (MQ=255)

                                                               aaacaaacCTGGCTCGGCACTTGAATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATGGCTCAGGCGGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                            <  3:165346/139‑1 (MQ=255)

                                                               aaaCCAAATTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAACGCTGGGACATTGCTCCGGCCGGTAAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                            <  8:327204/139‑1 (MQ=255)

                                                               aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGcc                                                                                                                               >  7:212764/1‑88 (MQ=255)

                                                               aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGcc                                                                                                                               <  6:265547/88‑1 (MQ=255)

                                                               aaaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                            >  1:146293/1‑139 (MQ=255)

                                                                aaCCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGtt                                                                           >  3:253058/1‑139 (MQ=255)

                                                                 aCCAAACGGGCGCGCCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCGGGGACATTGCTCAGGCGGGTTAATTT‑CGCGGCCCAACCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTg                                                                          <  8:41177/139‑1 (MQ=255)

                                                                      aCTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGCCATTAAATGCCAACGTTGCgg                                                                       >  4:237972/1‑137 (MQ=255)

                                                                          gcggggCACTTCCACCAGCGTGTTGAGCGGCAGCGATAAACGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                 <  2:53353/135‑1 (MQ=255)

                                                                          gcgcGGCACTTTCATCAGCGTGTTGAGCTGCAGCTATTAAAGCTGGGAAATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                 <  6:334607/136‑1 (MQ=255)

                                                                          gCTCGGAACTTACATCTCCCTTTTCTGGTGCTGTGATTAAAGCTGGGACTTTGAACAGGCCGTTTAATTT‑GGCGGCCAAATCCTTCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAgcgc                                                                 <  8:18156/139‑1 (MQ=255)

                                                                           cTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGt                                                                            <  1:63575/127‑1 (MQ=255)

                                                                                     tcatcaCCTGGTTGAGCTGCCGCGATAAACGGGGGTACTTTGGCCAGCCCTTTAATTTT‑CGCGGCCCATTCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                                       <  4:83751/138‑1 (MQ=255)

                                                                                     tcaccaGCGTGTTGCGCTGCCGCGATAAACGCGGGGACATTGCTCAGGCGGGTTAATTT‑CGCGGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                                       <  5:310713/138‑1 (MQ=255)

                                                                                            ccTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTAc                                               >  1:316313/1‑139 (MQ=255)

                                                                                                ttGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGgcg                                           >  7:294272/1‑139 (MQ=255)

                                                                                                      tGCAGCGATTAAAGCTGGGACATTGCTCAGGACGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCt                                     >  5:236801/1‑139 (MQ=255)

                                                                                                        cAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGATGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTgg                                   >  1:264216/1‑139 (MQ=255)

                                                                                                          gctaTTAACGCTGGGACTTTGCTCAGGCCGTTTAATTA‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                 <  4:129584/136‑1 (MQ=255)

                                                                                                          gctaTTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                 <  7:23777/136‑1 (MQ=255)

                                                                                                          gctaTTAAAGCGGGGACATTGCGCTGGCCGGTTAATTG‑CGCGGCCCAATCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                 <  3:323685/136‑1 (MQ=255)

                                                                                                          gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                 >  5:208152/1‑139 (MQ=255)

                                                                                                          gCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgct                                 >  5:112735/1‑139 (MQ=255)

                                                                                                            gtttAATGCGGGTACTTTGCCCCGGCCGTTAAATTG‑CGCGGCCCAAGCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttg                               <  2:96168/137‑1 (MQ=255)

                                                                                                             aTTAACGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGCCACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgc                              >  7:83449/1‑139 (MQ=255)

                                                                                                             aTTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGgcttgc                              >  6:290505/1‑139 (MQ=255)

                                                                                                                  cgCTGGGACATTGCTCAGGCCGGTTATTTT‑CGCGGCCCAATCCTGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAg                         <  7:47664/138‑1 (MQ=255)

                                                                                                                   gCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGa                        <  5:146496/139‑1 (MQ=255)

                                                                                                                       ggACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGCAccc                    >  2:116104/1‑139 (MQ=255)

                                                                                                                       ggACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAcccc                    >  1:100389/1‑139 (MQ=255)

                                                                                                                       ggACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc                    >  7:309293/1‑139 (MQ=255)

                                                                                                                       ggACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAAccc                    >  7:145351/1‑139 (MQ=255)

                                                                                                                             tGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt              >  3:80794/1‑139 (MQ=255)

                                                                                                                             tGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCAAGACACGACGCTGCACTCAGCAACACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGCCTTGCTTAAGAACCCTTCGCt              >  7:164039/1‑139 (MQ=255)

                                                                                                                             tGCTCAGGCCGGTTAATTT‑CGCGGCCCAAACCAGACACGACGCTTCACTCAGCATCACAGACATTAAATGACAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt              >  7:116112/1‑139 (MQ=255)

                                                                                                                              gCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt              <  5:159480/138‑1 (MQ=255)

                                                                                                                              gCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCt              <  7:183588/138‑1 (MQ=255)

                                                                                                                                  ggccgggTTATTTG‑CGCGCCCAAATCCCGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg         <  2:155793/134‑1 (MQ=255)

                                                                                                                                  aGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGCCAg         >  5:185030/1‑139 (MQ=255)

                                                                                                                                  aGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg         >  5:79171/1‑139 (MQ=255)

                                                                                                                                  aGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg         >  1:174344/1‑139 (MQ=255)

                                                                                                                                  aGGCCGGGTAATTT‑CGCGGCCCAATCCACCCACGTCGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg         <  8:278403/139‑1 (MQ=255)

                                                                                                                                   aggcGGTTAATTTGCGCGGCCCAAACCACACACGACGCTGCACTCAGCATCACAGACATTAAATGCCATCGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAg         <  4:168512/136‑1 (MQ=255)

                                                                                                                                    gCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTACGAACCCTTCGCTGACAGc        >  6:99114/1‑138 (MQ=255)

                                                                                                                                    gCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGc        >  8:234291/1‑138 (MQ=255)

                                                                                                                                    gCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGc        >  3:200804/1‑138 (MQ=255)

                                                                                                                                         ttAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCaa                                                          >  6:145581/1‑83 (MQ=255)

                                                                                                                                         ttAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAg                                                       <  1:191515/86‑1 (MQ=255)

                                                                                                                                         ttAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACgg                                             >  4:149850/1‑96 (MQ=255)

                                                                                                                                         ttAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa    >  6:309924/1‑137 (MQ=255)

                                                                                                                                         ttAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCaaaa    >  7:63745/1‑137 (MQ=255)

AAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAAAGCTGGGACATTGCTCAGGCCGGTTAATTT‑CGCGGCCCAATCCAGACACGACGCTGCACTCAGCATCACAGACATTAAATGCCAACGTTGCGGTAGCGCATGGCAAAAGCTATGTACGGCGCTTTCTGGCTTGCTTAAGAACCCTTCGCTGACAGCAAAATA  >  pSIJ663_pHT315_araE_xy/6559‑6834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`