| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,169,937 | A→G | 38.5% | H92R (CAT→CGT) | U712_05805 → | Transcriptional activator protein med |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,169,937 | 0 | A | G | 38.5% | 35.5 / 73.7 | 65 | H92R (CAT→CGT) | U712_05805 | Transcriptional activator protein med |
| Reads supporting (aligned to +/- strand): ref base A (21/19); new base G (9/16); total (30/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01 | |||||||||||
GGTATAAAGGATTACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGATAATGTAACAAGTGTCCATTTTAGCGGGGAAGCAATGGGCTTTTTTGG > CP006881/1169802‑1170075 | ggTATAAAGGATTACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATgg > 1:117626/1‑139 (MQ=255) taAATGATTACTTAAAATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAg < 2:131291/138‑1 (MQ=255) taAAGGATTACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGt < 5:75732/139‑1 (MQ=255) taAAGGATTACTGAATAAACAATCCAAATCCAATGTGGACGTCTATAATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGCGGATTTTCATAAGAGGGGCGGCAAGCTGCTCTACGGCCGGGGGAg < 6:69826/138‑1 (MQ=255) taGGATTACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTTCGCTACGGCCATGGGAGTGa < 8:222807/138‑1 (MQ=255) aaGGATTACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGa < 8:105425/139‑1 (MQ=255) ggATTACTGAATATACAATCCAAATACAATGTGGACGTCTATAATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAat < 6:264934/139‑1 (MQ=255) aTTACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCTATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAatat > 7:101185/1‑139 (MQ=255) tACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGc > 8:34983/1‑139 (MQ=255) tACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGc > 2:94304/1‑139 (MQ=255) tACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCAAGGGAGTGAATATGc > 6:69227/1‑139 (MQ=255) cTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAATGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATATGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTg < 2:288209/139‑1 (MQ=255) cTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTg < 7:236985/139‑1 (MQ=255) aaaataCCATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAACTGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAg < 2:112977/135‑1 (MQ=255) aaTATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTg < 3:283590/136‑1 (MQ=255) aaTATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTg > 4:283590/1‑136 (MQ=255) aaTATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAg < 2:117626/138‑1 (MQ=255) atatACAATCGAAATACAATGTGGACGTATATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATTAATGCGATTGTGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCttttgt < 4:19988/139‑6 (MQ=255) tataCAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGgtg > 3:288978/1‑139 (MQ=255) aaTCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTa > 8:307698/1‑138 (MQ=255) aaTCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATAATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATATGCTCTAAGGCCATGGGTGTGAATATTCTGAGGTGTTTa > 8:307117/1‑138 (MQ=255) aaaTACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTgg < 4:9123/139‑1 (MQ=255) cAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGc < 1:135302/139‑1 (MQ=255) cAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGc < 8:225989/139‑1 (MQ=255) cAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGc < 6:225652/139‑1 (MQ=255) aTGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGTATATGGGGAGGTGTTTAACGTGGTCGGCg > 8:114780/1‑138 (MQ=255) aTGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAg < 3:328983/119‑1 (MQ=255) aTGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAg > 4:328983/1‑119 (MQ=255) aTGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCg > 3:302982/1‑138 (MQ=255) aTGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCGCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCg > 3:247740/1‑138 (MQ=255) tgtgGACGTCTAATATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCCATCTGCTCTACGGCCAAGGGAGTGTATATGGTGAGGTGTTTTAATTGGTAGGCGaa > 6:314438/1‑139 (MQ=255) aCGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGACAATCTGCTCTACGGCCATGGGAGTGAATATTCTGAGGTGTTTAACTTGGTCGGCGAAGATTa > 7:178013/1‑139 (MQ=255) gtatattatAAAGAAGGCGTTAAAACACAAGAAGAAATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATc < 5:314438/136‑1 (MQ=255) gTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATc < 1:94304/139‑1 (MQ=255) gTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATc < 7:34983/139‑1 (MQ=255) gTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATc < 5:69227/139‑1 (MQ=255) ttatAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGat < 7:114780/139‑1 (MQ=255) aagaagGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTAATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATgt > 2:242137/1‑137 (MQ=255) gaagGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAAt < 3:10016/139‑1 (MQ=255) ggCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGGGTGAATTTGCGGGGGTGTTTGACGTGGGCGGCGCAGCTTATGCAGGTATGGAAttag > 4:235490/1‑137 (MQ=255) ggCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCg > 7:63623/1‑139 (MQ=255) gTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGa < 3:272843/116‑1 (MQ=255) gTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGa > 4:272843/1‑116 (MQ=255) gaagaagaTATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATg > 8:218837/1‑139 (MQ=255) atCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGGGTGTATTTGGTGGGGTGGTTTAATTGGGCGGGGAAGATTATACAGATATTGGATTAGTGATTTTCCATgggaaagcg > 5:177087/1‑138 (MQ=255) atCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATgcgaaagcg > 8:89598/1‑138 (MQ=255) tCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATgcgaaagcga < 7:89598/138‑1 (MQ=255) tCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATgcgaaagcgaa < 7:307117/139‑1 (MQ=255) tCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTAAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATgcgaaagcgaa < 7:307698/139‑1 (MQ=255) cATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATgcgaaagcga > 7:192636/1‑137 (MQ=255) cATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATgcgaaagcga > 3:226774/1‑137 (MQ=255) cATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATgcgaaagcga > 5:232527/1‑137 (MQ=255) aTAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGcgaaagcgaaag < 5:160598/139‑1 (MQ=255) aTGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCCTTTCCAATGCGAAAGCGAAAGCTGa > 8:68954/1‑139 (MQ=255) tGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTggg > 4:327115/1‑52 (MQ=255) tGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTggg < 3:327115/52‑1 (MQ=255) tGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGAt < 6:30580/139‑1 (MQ=255) tGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGAt < 8:63623/139‑1 (MQ=255) tGCGATTGAGGATTTTAATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGAt < 6:79341/139‑1 (MQ=255) acagATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGAt < 3:130332/136‑1 (MQ=255) aGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGATAATGTAAc < 7:218837/139‑1 (MQ=255) tCATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATACAGATATTGAATTAGTAATTTCCAATGCGAAAGCGAAAGCGGATAATTTAACAAgctgt > 4:130332/1‑135 (MQ=255) cATAAGAGGGGCGTCAATCTGCTCTACGGCCGTGGGAGTGAATATGCTGAGGGGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGATAATGTAACAAGTGTc < 8:21551/138‑1 (MQ=255) aTCTGCTCTACGGCCGTGGGGGTGAATATTGGGAGGTGTTTAACTTGGGCGGGGCAGATTTTCCAGATATGGAATTAGTCATTTGCAATGGGAAAGCGCAAGGTGATTATGTAACAAGTGTCCATTTTTGGGGGGGAGc > 3:126057/1‑139 (MQ=255) ctctACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGATAATGTAACAAGTGTCCATTTTAGCGGGGAAGCAATgg > 3:98625/1‑139 (MQ=255) aTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGATAATGTAACAAGTGTCCATTTTAGCGGGGAAGCAATGGGCTTTTTTgg < 4:288978/139‑1 (MQ=255) aTGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGATAATGTAACAAGTGTCCATTTTAGCGGGGAAGCAATGGGCTTTTTTg > 1:32724/1‑138 (MQ=255) | GGTATAAAGGATTACTGAATATACAATCCAAATACAATGTGGACGTCTATTATAAAGAAGGCGTTAAAACAGAAGAAGATATCATAAATGCGATTGAGGATTTTCATAAGAGGGGCGTCAATCTGCTCTACGGCCATGGGAGTGAATATGCTGAGGTGTTTAACTTGGTCGGCGAAGATTATCCAGATATGGAATTCGTCATTTCCAATGCGAAAGCGAAAGCTGATAATGTAACAAGTGTCCATTTTAGCGGGGAAGCAATGGGCTTTTTTGG > CP006881/1169802‑1170075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |