Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,211,125 A→G 5.4% K102E (AAG→GAG)  U712_06030 → Enoyl‑[acyl‑carrier‑protein] reductase [NADH] FabI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,211,1250AG 5.4% 225.0 / 3.9 74K102E (AAG→GAG) U712_06030Enoyl‑[acyl‑carrier‑protein] reductase [NADH] FabI
Reads supporting (aligned to +/- strand):  ref base A (39/31);  new base G (2/2);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CGCAACGATTCCATCATCCTCCCTTGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAGGCGGAAGCATTGTCACT  >  CP006881/1210993‑1211256
                                                                                                                                    |                                                                                                                                   
cGCAACGATTCCATCATCCTCCCTTGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTg                                                                                                                               >  4:13379/1‑139 (MQ=255)
   aaCGATTCCATCATCCTCCCTTGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCg                                                                                                                            >  4:132542/1‑139 (MQ=255)
        ttCCATCATCCTCCCTTGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAg                                                                                                                       <  4:304074/139‑1 (MQ=255)
                  ctccCTTGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAaca                                                                                                             >  4:58730/1‑139 (MQ=255)
                     ccTTGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAAcaca                                                                                                           >  5:152095/1‑138 (MQ=255)
                        tGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGTCGAGTACTTAAACACAAATc                                                                                                       >  8:223096/1‑139 (MQ=255)
                          cGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGt                                                                                                     >  3:205474/1‑139 (MQ=255)
                            aTGTTACACACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGa                                                                                                   <  6:136351/139‑1 (MQ=255)
                            aTGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTGAAACACAAATCGTGa                                                                                                   >  1:307175/1‑139 (MQ=255)
                               ttACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAg                                                                                                                       <  3:179495/116‑1 (MQ=255)
                               ttACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAg                                                                                                                       >  4:179495/1‑116 (MQ=255)
                                  cAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCt                                                                                              <  4:310409/138‑1 (MQ=255)
                                    aaCGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTcc                                                                                           >  2:237809/1‑139 (MQ=255)
                                       gacgCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAGAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCttt                                                                                        >  1:47191/1‑139 (MQ=255)
                                       gacgCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACCGCTTCCttt                                                                                        <  8:328863/139‑1 (MQ=255)
                                         cgCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCttt                                                                                        <  1:318822/137‑1 (MQ=255)
                                         cgCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCttt                                                                                        >  2:318822/1‑137 (MQ=255)
                                         cgCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTg                                                                                      >  7:30353/1‑139 (MQ=255)
                                          gctgAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCTGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTgg                                                                                     <  3:13379/136‑1 (MQ=255)
                                          gCAGAAATCGAAACTTTCTTCGCAAGAATTAATGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTAGCGCGTTTGGCATCATAGAAATGCTTGTAGGCGAGTACTTAAACACAAATCGTGACGGCTTACTTTTgg                                                                                     <  2:240366/139‑1 (MQ=255)
                                            aGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTGCCTTTTGGCt                                                                                   <  3:80059/139‑1 (MQ=255)
                                                 tCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGc                                                                                                                          <  7:71520/95‑1 (MQ=255)
                                                 tCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGc                                                                                                                          >  8:71520/1‑95 (MQ=255)
                                                   gAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAAc                                                                             <  4:205474/138‑1 (MQ=255)
                                                     aaCTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAGAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATc                                                                          <  7:224333/139‑1 (MQ=255)
                                                     aaCTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAGAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATc                                                                          <  2:47191/139‑1 (MQ=255)
                                                     aaCTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATc                                                                          <  2:107074/139‑1 (MQ=255)
                                                       cTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAg                                                                        <  1:245342/139‑1 (MQ=255)
                                                       cTTGCGTCGCAAGCATTAAGCAGCAGGTCGGTGTAATCCACGGTATCGCGCAGGGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAg                                                                        <  8:214104/139‑1 (MQ=255)
                                                           cTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCa                                                                    >  8:294167/1‑139 (MQ=255)
                                                              cGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCata                                                                  <  6:152095/138‑1 (MQ=255)
                                                                 aaGCATTAAGGAGCATGTCGGTGTAATCCACGGTATAGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATtct                                                              <  3:220483/139‑1 (MQ=255)
                                                                          ggAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGActgct                                                     <  5:71311/139‑1 (MQ=255)
                                                                          ggAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGActgct                                                     <  3:132542/139‑1 (MQ=255)
                                                                          ggAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGActgct                                                     <  3:58730/139‑1 (MQ=255)
                                                                              cAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAACTCGTGACGGCTTCCTTTTGGCTCATAACATCCGCTCCTATTCTCTGACTGCtgttg                                                 >  5:255786/1‑139 (MQ=255)
                                                                              cAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGAGGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCtgttg                                                 >  1:10658/1‑139 (MQ=255)
                                                                              cAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCtgttg                                                 >  3:163152/1‑139 (MQ=255)
                                                                              cAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCtgttg                                                 >  8:49725/1‑139 (MQ=255)
                                                                              cAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCtgttg                                                 >  1:242809/1‑139 (MQ=255)
                                                                                      tgtAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTg                                                                                      <  3:17479/94‑1 (MQ=255)
                                                                                      tgtAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTg                                                                                      >  4:17479/1‑94 (MQ=255)
                                                                                      tgtAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCat                                                                   >  1:114473/1‑113 (MQ=255)
                                                                                      tgtAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCat                                                                   <  2:114473/113‑1 (MQ=255)
                                                                                      tgtAATCAACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCg                                         >  1:168053/1‑139 (MQ=255)
                                                                                        tAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGc                                       <  1:225889/139‑1 (MQ=255)
                                                                                           tCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGa                                                          <  4:50885/117‑1 (MQ=255)
                                                                                           tCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGa                                                          >  3:50885/1‑117 (MQ=255)
                                                                                           tCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACg                                    >  3:77029/1‑139 (MQ=255)
                                                                                                ggTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAGAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCga                               >  7:52797/1‑139 (MQ=255)
                                                                                                ggTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGc                                                                                                                          >  3:235620/1‑48 (MQ=255)
                                                                                                ggTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGc                                                                                                                          <  4:235620/48‑1 (MQ=255)
                                                                                                ggTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAg                                                                                                                       >  5:51482/1‑51 (MQ=255)
                                                                                                ggTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAg                                                                                                                       >  5:47296/1‑51 (MQ=255)
                                                                                                ggTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAg                                                                                                                       <  6:51482/51‑1 (MQ=255)
                                                                                                ggTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAg                                                                                                                       <  6:47296/51‑1 (MQ=255)
                                                                                                    tCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCgatgat                           <  8:42487/139‑1 (MQ=255)
                                                                                                     cgcgCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCAt                                                                                >  4:108921/1‑85 (MQ=255)
                                                                                                     cgcgCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCAt                                                                                <  3:108921/85‑1 (MQ=255)
                                                                                                     cgcgCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCgatgat                           >  6:269991/1‑138 (MQ=255)
                                                                                                     cgcgCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCgatgat                           >  5:15735/1‑138 (MQ=255)
                                                                                                     cgcgCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCgatgat                           >  6:166254/1‑138 (MQ=255)
                                                                                                     cgcgCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTGTTGGCTCATAACATCAGCTCATATACTCTGACTGCTGTTGTCAAAGCGGCACGTCCgatgat                           >  5:63987/1‑138 (MQ=255)
                                                                                                            tGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTctct                                                            <  6:169149/98‑1 (MQ=255)
                                                                                                            tGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTctct                                                            >  5:169149/1‑98 (MQ=255)
                                                                                                            tGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAg                   >  4:176877/1‑139 (MQ=255)
                                                                                                              tATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAGGc                 >  7:138263/1‑139 (MQ=255)
                                                                                                                   cgTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCgatg                             >  5:132399/1‑122 (MQ=255)
                                                                                                                   cgTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCgatg                             <  6:132399/122‑1 (MQ=255)
                                                                                                                      ttGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAGGCGGAAGCAt         >  6:221245/1‑139 (MQ=255)
                                                                                                                        gCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGCCGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAGGCGGAAGCATTg       >  6:201724/1‑139 (MQ=255)
                                                                                                                        gCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAGGCGGAAGCATTg       >  3:165086/1‑139 (MQ=255)
                                                                                                                             caaagcaaagCTTGGCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAGGCGGAAGCATTGTCACt  <  5:217041/139‑1 (MQ=255)
                                                                                                                             caaAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAgg                  <  3:130697/123‑1 (MQ=255)
                                                                                                                             caaAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAgg                  >  4:130697/1‑123 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CGCAACGATTCCATCATCCTCCCTTGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGCATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAAAGCTTGTCGGCGAGTACTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCACGTCCGATGATGACTGAAGGCGGAAGCATTGTCACT  >  CP006881/1210993‑1211256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑