Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,652,231 A→G 5.8% N173S (AAC→AGC)  U712_08490 → ATP‑dependent protease ATPase subunit ClpY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,652,2310AG 5.8% 196.6 / 2.3 68N173S (AAC→AGC) U712_08490ATP‑dependent protease ATPase subunit ClpY
Reads supporting (aligned to +/- strand):  ref base A (22/42);  new base G (2/2);  total (24/44)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGCAAACAAACGCATTGTTCGTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTATGGAGCAGA  >  CP006881/1652095‑1652365
                                                                                                                                        |                                                                                                                                      
aaTGCAAACAAACGCATTGTTCGTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAAc                                                                                                                                       >  3:274422/1‑138 (MQ=255)
aaTGCAAACAAACGCATTGTTCGTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACa                                                                                                                                      >  3:31591/1‑139 (MQ=255)
 aTGCAAACTAACGCATTGTTCGTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATCAGGCGGAGAGCCAGGAAGAAGCAAACAt                                                                                                                                     >  3:104137/1‑139 (MQ=255)
 aTGCAAACAAACGCATTGTTCGTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACAt                                                                                                                                     >  8:288089/1‑139 (MQ=255)
         aaacGCATTGTTCGTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCgaagaa                                                                                                                              >  5:139324/1‑138 (MQ=255)
                 tGTTCGTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGaagaaaaaagaaaa                                                                                                                     >  7:278392/1‑139 (MQ=255)
                      gTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGATGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAAt                                                                                                                >  1:132172/1‑139 (MQ=255)
                       tCTGTTTGTTCCTTGGAAGAAAAAACAATATGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATg                                                                                                               <  6:81238/139‑1 (MQ=255)
                       tCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATg                                                                                                               >  1:248406/1‑139 (MQ=255)
                           ttAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGgcg                                                                                                            >  5:100422/1‑138 (MQ=255)
                                tCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCTAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAg                                                                                                      <  6:43871/139‑1 (MQ=255)
                                 ccTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGTATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAg                                                                                                      <  8:141664/138‑1 (MQ=255)
                                 ccTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAg                                                                                                      <  4:140999/138‑1 (MQ=255)
                                  cTGTGAATAAAAAACAATCTTGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCTGATTGGTGAATATGTGGGGGAGAGCCAGGAATAATCAAACATCGAAGAATAAAGAAAACGAATGGCGCATCAGCt                                                                                                    <  1:125688/139‑1 (MQ=255)
                                  cTGGGTATAAAAAACAATCTTGTTTTAAATATCAGTTTGATATTTTTTTTGGAGGAAGCCAGACGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCt                                                                                                    <  8:278392/139‑1 (MQ=255)
                                  cTGGGAAGAAAACACAAGCTGGTGTTAAACATCCGTTTGAAATGGTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCt                                                                                                    <  2:282401/139‑1 (MQ=255)
                                  cTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGATGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCt                                                                                                    <  2:132172/139‑1 (MQ=255)
                                  cTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCt                                                                                                    >  5:109433/1‑139 (MQ=255)
                                  atGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTATTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACCAATGGCGCATCAGCt                                                                                                    <  2:279216/138‑1 (MQ=255)
                                   tGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAGCATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTg                                                                                                   >  3:157463/1‑139 (MQ=255)
                                   tGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAGCATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTg                                                                                                   >  3:162867/1‑139 (MQ=255)
                                     ggAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGgcg                                                                                                            >  1:227788/1‑128 (MQ=255)
                                     ggAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGgcg                                                                                                            <  2:227788/128‑1 (MQ=255)
                                     ggAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGc                                                                                                 >  1:287102/1‑139 (MQ=255)
                                             aaaCAATCTGGTTTTAAAAATCTGTTTGAAATGTTTTTTGGAGGCCGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGgag                                                                                         <  1:151750/139‑1 (MQ=255)
                                             aaaCAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGgag                                                                                         <  2:248406/139‑1 (MQ=255)
                                                cAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGc                                                                                      <  6:121106/139‑1 (MQ=255)
                                                cAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGc                                                                                      <  5:75275/139‑1 (MQ=255)
                                                      tgtgtTAAAAATCCTTTTGAAATTTTTTTTGGGGTCAGCCAGCAGTATGGTTAATATGAGGCGGAGAGCCAGGAAGAATCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGaa                                                                                 <  7:143860/137‑1 (MQ=255)
                                                       gtgtTAAAAATCGGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCg                                                                                   <  7:104026/135‑1 (MQ=255)
                                                       gtgtTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGACGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGaa                                                                                 <  6:67524/137‑1 (MQ=255)
                                                       gtgtTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGaa                                                                                 <  3:122217/137‑1 (MQ=255)
                                                       gtgtTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGaa                                                                                 <  7:11337/137‑1 (MQ=255)
                                                        tttaaaaaaTCCGTTTGAAATGTTTTTTGGAGGCAGCAAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGac                                                                              <  8:288239/135‑1 (MQ=255)
                                                        tgtTAAAAATCCGTTTGTAATGTTTTTTGGAGGCAGCGAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGac                                                                              <  5:169081/139‑1 (MQ=255)
                                                        tgtTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGac                                                                              <  2:83011/139‑1 (MQ=255)
                                                        tgtTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGac                                                                              <  7:288089/139‑1 (MQ=255)
                                                          ttAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCTGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGacta                                                                            <  4:139527/139‑1 (MQ=255)
                                                          ttAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGacta                                                                            <  8:198107/139‑1 (MQ=255)
                                                            aaaaaTCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGactac                                                                           >  5:167476/1‑138 (MQ=255)
                                                             aaaaTCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGActacta                                                                         >  6:70384/1‑139 (MQ=255)
                                                                   cGTTTTAAATGTTTTTTGGCGGCCGCCAGCGTAATGGTGAAGAGGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTaa                                                                    <  2:324510/138‑1 (MQ=255)
                                                                          aaTGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGa                                                             <  6:100422/138‑1 (MQ=255)
                                                                                      aGGCAGCCAGCCGAATGGTGAAGATGCGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAACAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAAcag                                                <  1:199438/139‑1 (MQ=255)
                                                                                        gCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAAcag                                                >  1:48849/1‑137 (MQ=255)
                                                                                               gccGATTGGTGCAGTTGCGGGGGAGAGCCAGGATGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGTAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCt                                       <  3:220598/139‑1 (MQ=255)
                                                                                                         gAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACa                             <  4:31591/139‑1 (MQ=255)
                                                                                                          aaGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACa                             <  5:70384/138‑1 (MQ=255)
                                                                                                          aaGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACa                             <  6:167476/138‑1 (MQ=255)
                                                                                                             aTGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACAtgct                         >  8:301744/1‑139 (MQ=255)
                                                                                                             aTGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACAtgct                         >  7:204666/1‑139 (MQ=255)
                                                                                                             aTGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACAtgct                         >  2:196545/1‑139 (MQ=255)
                                                                                                                 ggCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAg                     >  3:21235/1‑139 (MQ=255)
                                                                                                                  gCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAgg                    >  4:79547/1‑139 (MQ=255)
                                                                                                                    ggAGAGCCAGGAAGAACCAAACATCGAAGAAAAAAGAAAACGACTGGCGCATCAGCGGGCTTTAGGAGAGCTCGAAGACTACTTTGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGc                  <  4:274422/139‑1 (MQ=255)
                                                                                                                      agagCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAg                                                                                                      >  1:261134/1‑53 (MQ=255)
                                                                                                                      agagCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAg                                                                                                      <  2:261134/53‑1 (MQ=255)
                                                                                                                      agagCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGActacta                                                                         <  4:10445/82‑1 (MQ=255)
                                                                                                                      agagCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGActacta                                                                         >  3:10445/1‑82 (MQ=255)
                                                                                                                      agagCCAGGAAGAAGCAAACATCGAAGAAAAAACAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTc                <  4:23241/139‑1 (MQ=255)
                                                                                                                           cAGGAAGAAGCAAGCATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTa           <  4:162867/139‑1 (MQ=255)
                                                                                                                           cAGGAAGAAGCAAGCATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTa           <  4:157463/139‑1 (MQ=255)
                                                                                                                           cAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTa           <  1:196545/139‑1 (MQ=255)
                                                                                                                           cAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTa           <  7:301744/139‑1 (MQ=255)
                                                                                                                                   agCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTATGGAGCAg   <  8:204666/139‑1 (MQ=255)
                                                                                                                                    gCAAACATCTAACAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTATGGAGCAGa  <  1:56121/139‑1 (MQ=255)
                                                                                                                                    gCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTATGGAGCAGa  <  7:72476/139‑1 (MQ=255)
                                                                                                                                    gCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTATGGAGCAGa  <  3:142879/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
AATGCAAACAAACGCATTGTTCGTCTGTTAGTTCCTGGGAAGAAAAAACAATCTGGTGTTAAAAATCCGTTTGAAATGTTTTTTGGAGGCAGCCAGCCGAATGGTGAAGATGAGGCGGAGAGCCAGGAAGAAGCAAACATCGAAGAAAAAAGAAAACGAATGGCGCATCAGCTGGCTTTAGGAGAGCTCGAAGACTACTATGTAACAGTAGAAGTCGAAGAACAGCAGCCTTCTATGTTTGACATGCTGCAGGGCTCGGGTATGGAGCAGA  >  CP006881/1652095‑1652365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑