Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,667,358 G→A 56.5% W37* (TGG→TAG)  U712_08585 → Flagellar biosynthetic protein fliZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,667,3580GA56.5% 10.5 / 71.6 60W37* (TGG→TAG) U712_08585Flagellar biosynthetic protein fliZ
Reads supporting (aligned to +/- strand):  ref base G (16/9);  new base A (21/12);  total (37/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

TAAATTAAAGGGTGTACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCTTTTGATTTTGTAAAGATGATTTTCGCT  >  CP006881/1667227‑1667494
                                                                                                                                   |                                                                                                                                        
taaattaaaGGGTGTACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCa                                                                                                                                    >  5:28179/1‑138 (MQ=255)
    ttaaaGGGTGTACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAaaaga                                                                                                                               <  6:218782/139‑1 (MQ=255)
    ttaaaGGGGGTACGTCTGTTGAAATAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAataga                                                                                                                               <  6:28179/139‑1 (MQ=255)
            tgtACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGa                                                                                                                        >  8:247822/1‑138 (MQ=255)
            tgtACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGa                                                                                                                        >  3:325164/1‑138 (MQ=255)
              tACGACTGTTGAAAAAGATTATATATTTTATTGTTTTTTTTTGTTTTTTCGTTTTATTTAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTTTAAACGAATGGTTTCAAAAGAAAGATGaaa                                                                                                                      <  2:130578/138‑1 (MQ=255)
              tACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGaaaa                                                                                                                     <  7:215885/139‑1 (MQ=255)
              tACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGaaa                                                                                                                      >  7:122720/1‑138 (MQ=255)
              tACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGaaa                                                                                                                      >  2:36415/1‑138 (MQ=255)
              tACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGaaa                                                                                                                      >  7:325300/1‑138 (MQ=255)
              tACGACTGTTGAAAAAGAGTCAATAATTTATTGTTTTTATTTGTTTTTTCGGTTTATTCAGTGTACCTCCGATTGCTGCGGCCGCGGCAGACTCTGATAATTCAACTGTAAAAGAATACTTTCAAAACCAAGATGaaaa                                                                                                                     <  8:213582/139‑1 (MQ=255)
                  aCTGTTGAAAAAGACTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACt                                                                                                                 <  3:30797/139‑1 (MQ=255)
                   cTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                <  1:205310/139‑1 (MQ=255)
                      ttGAAAAAGCGTCAATTTTTTATTGTTTTTATTTGTTTTTTCGTGTTAATCAGTGTACATCCGATTGCTGCTGCCGCGGCAGCCTCTGAAAATTCACCTGTAAACGAATAGTTTCAAAAGAAAGAGCAAAAACCTg                                                                                                                >  1:89006/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATGCGATTGCTGCTGCCGCGGCAGCCTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                >  7:292906/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                >  6:78774/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                >  6:83681/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                >  7:329055/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                >  8:163975/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                >  2:68231/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                >  2:205310/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTg                                                                                                                >  2:128117/1‑136 (MQ=255)
                      ttGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGAGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAATATGAAAAAAATg                                                                                                                >  4:190915/1‑136 (MQ=255)
                        gAAAAAGAGTAAATATTTTATTGTTTTTATTTTTTTTTTCGTTTTATTCAGTTTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGa                                                                                                            <  2:228502/138‑1 (MQ=255)
                                gTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGatcaatca                                                                                                     >  1:258635/1‑137 (MQ=255)
                                gTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGatcaatca                                                                                                     >  1:228502/1‑137 (MQ=255)
                                        tttATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAaaag                                                                                            <  8:244298/138‑1 (MQ=255)
                                         ttATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAaaaga                                                                                           >  5:324836/1‑138 (MQ=255)
                                                    aTTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAaaca                                                                                <  4:325164/138‑1 (MQ=255)
                                                                 ttttATTATTTGTACATCCGATTGCTGATGACGCGGCAGACGCTGATAATTCAAATGTAAACGAATTGTTTCAAAATAAAGATTAAAAAAATTCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAActgctg                                                                   <  4:222025/138‑1 (MQ=255)
                                                                   ttATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCtgat                                                                 <  8:329055/138‑1 (MQ=255)
                                                                   ttATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCtgat                                                                 <  5:83681/138‑1 (MQ=255)
                                                                   ttATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCtgat                                                                 <  5:78774/138‑1 (MQ=255)
                                                                         aGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACg                                                           <  3:296634/138‑1 (MQ=255)
                                                                           tgtACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACggag                                                        >  5:165967/1‑139 (MQ=255)
                                                                           tgtACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACggag                                                        >  8:171158/1‑139 (MQ=255)
                                                                           tgtACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACggag                                                        >  7:124712/1‑139 (MQ=255)
                                                                           tgtACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATCAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACggag                                                        >  3:332826/1‑139 (MQ=255)
                                                                             tACATCCGATTGCGGCGGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAATAACAACAAAAACTGCTGATGAGACggaggg                                                      <  4:16468/139‑1 (MQ=255)
                                                                                aTCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGc                                                   >  7:164453/1‑139 (MQ=255)
                                                                                    gATTGCTGCTGCCGCGGCAGCCTCTGATAATTCAACTGGAAACGAATAGTTTCAAAAGAAAGATGAAAAACCTGCAGATCAATCAGCGCAAAAGAAAGAAAAAACAACAAACACTGCTGATGAGACGGAGGGAGCGgct                                               >  7:294197/1‑139 (MQ=255)
                                                                                     aTTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAACCAACAAAAACTGCTGATGAGCCGGAGGGAGCGgctg                                              >  8:174346/1‑139 (MQ=255)
                                                                                     aTTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGgctg                                              <  6:126664/139‑1 (MQ=255)
                                                                                     aTTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGgctg                                              <  6:125988/139‑1 (MQ=255)
                                                                                     aTTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGgctg                                              <  6:324836/139‑1 (MQ=255)
                                                                                     aTTGCTGCTGCCGCGGCAGACTCTGATAAATCAACTGTAAACGAATAGTTTCAAAAGAAAGATCAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGgctg                                              >  6:167641/1‑139 (MQ=255)
                                                                                       tgctgctgcCGCGGCAGACACTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGCGGGAGCGgctgct                                            >  4:208762/1‑139 (MQ=255)
                                                                                            ctgcCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCtt                                        <  6:165967/138‑1 (MQ=255)
                                                                                            ctgcCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTc                                       >  2:969/1‑139 (MQ=255)
                                                                                            ctgcCGCGGCAGACTCTGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTc                                       >  3:143718/1‑139 (MQ=255)
                                                                                             tgcCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGAACAATAAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCAGATGAGACGGAGGGAGCGTCTGCGCCATCt                                      >  2:330013/1‑139 (MQ=255)
                                                                                                 gcctcAGACTCTTATAATTCAACTGTTAACTAATTGTTTCAAAAGAAAGATGAAAAAACTGCATAACAATCAGAGCAAAAGAAACAAAAAACAACAAAAACTGCTGATGAGACGGTGGGAGCGGCTGCTCCTTCTGtct                                  <  1:330013/135‑1 (MQ=255)
                                                                                                         ctctGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCtttt                          >  3:312878/1‑139 (MQ=255)
                                                                                                         ctctGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCtttt                          >  7:32195/1‑139 (MQ=255)
                                                                                                          tctGATAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCtttt                          >  8:141808/1‑138 (MQ=255)
                                                                                                              aTAATTCAACTGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCTTTTGAtt                      <  6:119602/138‑1 (MQ=255)
                                                                                                                      aCTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCTTTTGATTTTGTAAAgc             >  2:298907/1‑138 (MQ=255)
                                                                                                                        tGTAAACGAATAGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCTTTTGATTTTGTAAAgat            >  4:89091/1‑138 (MQ=255)
                                                                                                                          tAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCTTTTGATTTTGTAAAgatgat         <  7:163975/139‑1 (MQ=255)
                                                                                                                                 aTGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCTTTTGATTTTGTAAAGATGATTTTCGCt  >  5:116447/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                        
TAAATTAAAGGGTGTACGACTGTTGAAAAAGAGTCAATATTTTATTGTTTTTATTTGTTTTTTCGTTTTATTCAGTGTACATCCGATTGCTGCTGCCGCGGCAGACTCTGATAATTCAACTGTAAACGAATGGTTTCAAAAGAAAGATGAAAAAACTGCAGATCAATCAGAGCAAAAGAAAGAAAAAACAACAAAAACTGCTGATGAGACGGAGGGAGCGGCTGCTCCTTCTGTCTCAGCTTTTGATTTTGTAAAGATGATTTTCGCT  >  CP006881/1667227‑1667494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑