Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,871,087 A→T 5.6% T186S (ACA→TCA)  U712_09340 → putative membrane protein yndD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,871,0870AT 5.6% 224.2 / 2.9 72T186S (ACA→TCA) U712_09340putative membrane protein yndD
Reads supporting (aligned to +/- strand):  ref base A (46/22);  new base T (2/2);  total (48/24)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CTTCTCATCGATGGAATACCGCAGGCCTTTCTTCTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAGT  >  CP006881/1870952‑1871220
                                                                                                                                       |                                                                                                                                     
cgtctcATCGATGGAATACCGCAGGCCTTTCTTCTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGGCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAg                                                                                                                                    <  6:110836/137‑1 (MQ=255)
     cATCGATGGAATACCGCAGGCCTTTCTTCTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCtt                                                                                                                               >  7:204773/1‑139 (MQ=255)
        cGATGGAATACCGCAGGCCTTTCTTCTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGCGGGCCGCGCCCAGGCGTTACAGAAGAGTTGAGTACGAATACAGCTCtttt                                                                                                                             >  6:83166/1‑138 (MQ=255)
         gATGGAATACCGCAGGGCTTTCTTCTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTga                                                                                                                           <  5:104766/139‑1 (MQ=255)
          aTGGAATACCGCAGGCCTTTCTTCTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCCCAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTgag                                                                                                                          >  5:266224/1‑139 (MQ=255)
                     cAGGCCTTTCTTCTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCGTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGa                                                                                                                >  6:162460/1‑138 (MQ=255)
                          cTTTCTTCTTGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCTCAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAAt                                                                                                          <  1:162715/139‑1 (MQ=255)
                            ttcttcTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGCCAGCAAGGAAAAAATg                                                                                                         >  4:259790/1‑138 (MQ=255)
                             tcttctGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCCCAGGCGTTACAGAAGAGTTGAGTACGAATACCGCACGTTTGAGACAGCAAGGAAAAAATGa                                                                                                        >  4:70797/1‑138 (MQ=255)
                             tcttctGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGAt                                                                                                       >  3:140079/1‑139 (MQ=255)
                             tcttctGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGAGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTGATGAGACAGCAAGGAAAAAATGa                                                                                                        >  4:64434/1‑138 (MQ=255)
                                   gggAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAAtt                                                                                                  >  8:172830/1‑138 (MQ=255)
                                    ggATCTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGTAAAAAATGATCAATTaa                                                                                                <  3:287976/139‑1 (MQ=255)
                                         tGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAACGCTTATCAGAGGGCCGCGCCAAGCCTTTACAAAAAATATGACTACAAATACACATCATATCAAACAGCAAGTAAAAACTGATAAAATaaaaatg                                                                                           >  4:62906/1‑134 (MQ=255)
                                           tAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCCCAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTGTTTTGAGAAAGCAAGGAAAAAAAGATAAATTAACaatt                                                                                           >  3:12822/1‑134 (MQ=255)
                                            aaaaaaaCAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCag                                                                                        >  1:73933/1‑139 (MQ=255)
                                              aaaaaCAAAATCGAAGTATCGAGGAGCCGCTTTCAGAATAGATTTTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCa                                                                                         >  8:310252/1‑136 (MQ=255)
                                                   cAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGCATACCGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGAttt                                                                                  >  6:168436/1‑138 (MQ=255)
                                                   cAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATTCAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGAttt                                                                                  >  8:304740/1‑138 (MQ=255)
                                                   cAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATTCAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGAttt                                                                                  >  8:308986/1‑138 (MQ=255)
                                                    aaaaTCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTg                                                                                 >  2:118066/1‑138 (MQ=255)
                                                            aGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGaa                                                                        >  7:260782/1‑139 (MQ=255)
                                                                          gCTTTCAGAAGCGCTTGTCAGCGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAg                                                          >  7:318489/1‑139 (MQ=255)
                                                                          gCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAg                                                          >  2:179213/1‑139 (MQ=255)
                                                                                     cgcTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTAt                                               >  3:123160/1‑139 (MQ=255)
                                                                                          gTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGc                                            <  5:13064/137‑1 (MQ=255)
                                                                                            cAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACCGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTtat                                        <  6:266224/139‑1 (MQ=255)
                                                                                               agGGCCGCGCCCAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGCCAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTatat                                      >  1:148800/1‑138 (MQ=255)
                                                                                               agGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATatg                                     <  7:310252/139‑1 (MQ=255)
                                                                                               agGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATAGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTatat                                      >  4:101413/1‑138 (MQ=255)
                                                                                                 ggCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCa                                                                                         <  7:13843/85‑1 (MQ=255)
                                                                                                 ggCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCa                                                                                         >  8:13843/1‑85 (MQ=255)
                                                                                                 ggCCGCGCACAAGCTTTACAGAAGAGGTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACCCGATTAAAAAAGGATTTAATTATCGCTTATatgaa                                   <  3:148600/139‑1 (MQ=255)
                                                                                                    cgcgCACAGGCTTTACAGAACAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATCATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATaagaatga                                >  8:167353/1‑139 (MQ=255)
                                                                                                      cgcACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACa                              >  6:152666/1‑139 (MQ=255)
                                                                                                       gcACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACAt                             >  6:56402/1‑139 (MQ=255)
                                                                                                          caGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGc                          <  4:140079/139‑1 (MQ=255)
                                                                                                           attcTTTACATAATATTTTAGTAAGATTACAGCTATTTTGAGACAGAAAGGAAAAAATGATCAATTAAAATTTCAGAGATTTTAAGTAGGTACTAGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTgaa                         <  8:90353/136‑3 (MQ=255)
                                                                                                           aGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCa                         <  8:90141/139‑1 (MQ=255)
                                                                                                                 tACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAAt                                                                                                   >  3:290585/1‑59 (MQ=255)
                                                                                                                 tACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAAt                                                                                                   <  4:290585/59‑1 (MQ=255)
                                                                                                                 tACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATcc                    >  3:213163/1‑138 (MQ=255)
                                                                                                                 tACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATcc                    >  3:152094/1‑138 (MQ=255)
                                                                                                                 tACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATCAACAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATcc                    >  1:256592/1‑138 (MQ=255)
                                                                                                                   cAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCaa                  <  7:172830/138‑1 (MQ=255)
                                                                                                                    agaagaGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAATTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCaaaa                <  4:12822/139‑1 (MQ=255)
                                                                                                                       agagTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTaa                                                                                                >  6:122453/1‑56 (MQ=255)
                                                                                                                       agagTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTaa                                                                                                <  5:122453/56‑1 (MQ=255)
                                                                                                                       agagTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAag               >  2:246780/1‑137 (MQ=255)
                                                                                                                       agagTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAag               >  7:295481/1‑137 (MQ=255)
                                                                                                                        gagTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAagtct            >  7:90353/1‑137 (MQ=255)
                                                                                                                        gagTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAagtag            >  8:108832/1‑139 (MQ=255)
                                                                                                                         agTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTaa                                                                                                <  6:183116/54‑1 (MQ=255)
                                                                                                                         agTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTaa                                                                                                >  5:183116/1‑54 (MQ=255)
                                                                                                                           ttGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTc          >  5:111369/1‑138 (MQ=255)
                                                                                                                           ttGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTc          >  6:38798/1‑138 (MQ=255)
                                                                                                                           ttGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTc          >  7:299316/1‑138 (MQ=255)
                                                                                                                           ttGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTc          >  5:336443/1‑138 (MQ=255)
                                                                                                                           ttGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTc          >  2:184250/1‑138 (MQ=255)
                                                                                                                           ttGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAAAATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTc          >  3:246798/1‑138 (MQ=255)
                                                                                                                              aGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAg       >  3:47257/1‑138 (MQ=255)
                                                                                                                               gTACGAATACATCTCTTTTGAGACAGCAACGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAgg      <  7:154956/138‑1 (MQ=255)
                                                                                                                               gTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAgg      <  8:260782/138‑1 (MQ=255)
                                                                                                                                tACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAgg      >  8:324117/1‑137 (MQ=255)
                                                                                                                                tACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAgg      >  5:139783/1‑137 (MQ=255)
                                                                                                                                  cGAATTCAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAGt  <  7:304740/139‑1 (MQ=255)
                                                                                                                                  cGAATTCAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAGt  <  7:308986/139‑1 (MQ=255)
                                                                                                                                  cGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAg   >  2:202830/1‑138 (MQ=255)
                                                                                                                                  cGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAGt  <  7:324117/139‑1 (MQ=255)
                                                                                                                                  cGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAGt  <  5:38798/139‑1 (MQ=255)
                                                                                                                                  cGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAGt  <  1:179213/139‑1 (MQ=255)
                                                                                                                                     aTACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAGt  >  5:218764/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CTTCTCATCGATGGAATACCGCAGGCCTTTCTTCTGGGAACTGTAAAAAAACAAAATCGAAGTATCGAGGAGCCGCTTTCAGAAGCGCTTGTCAGAGGGCCGCGCACAGGCTTTACAGAAGAGTTGAGTACGAATACAGCTCTTTTGAGACAGCAAGGAAAAAATGATCAATTAACATTGCAGAGATTTGAAGTAGGAACACGATTAAAAAAGGATTTAATTATCGCTTATATGAATGACATTGCAGATCCAAAAGTAGTCGAGGAAGT  >  CP006881/1870952‑1871220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑