Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,175,293 A→G 13.0% V257V (GTT→GTC)  U712_10880 ← putative glycosyltransferase ypjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,175,2930AG13.0% 109.7 / 9.7 46V257V (GTT→GTC) U712_10880putative glycosyltransferase ypjH
Reads supporting (aligned to +/- strand):  ref base A (21/19);  new base G (3/3);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACG  >  CP006881/2175160‑2175427
                                                                                                                                     |                                                                                                                                      
ttCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTg                                                                                                                                   <  2:124697/139‑1 (MQ=255)
     aTACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTAATGAATTCCAAGCATTAAAACTTTGtct                                                                                                                               >  6:261879/1‑138 (MQ=255)
         aaGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTaa                                                                                                                          <  4:255288/139‑1 (MQ=255)
           ggCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGtctt                                                                                                                              <  6:11728/133‑1 (MQ=255)
           ggCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGtctt                                                                                                                              >  5:11728/1‑133 (MQ=255)
           ggCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATc                                                                                                                        >  1:45334/1‑139 (MQ=255)
               cTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCata                                                                                                                    >  1:97905/1‑139 (MQ=255)
                        ccATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGAc                                                                                                           <  2:45334/139‑1 (MQ=255)
                            cGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGc                                                                                                       >  3:155089/1‑139 (MQ=255)
                               cTCAAGAACGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTca                                                                                                    <  1:234198/139‑1 (MQ=255)
                                 cAAGCAGGACAAGGCCAAAGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTcg                                                                                                    >  4:141010/1‑136 (MQ=255)
                                 cAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTca                                                                                                    >  4:191414/1‑137 (MQ=255)
                                  aagaagGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTAGGTCTTCTAATCCATATTTTCTGACAAGCTCACAg                                                                                                 >  6:111662/1‑139 (MQ=255)
                                          cAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGa                                                                                         <  7:103762/139‑1 (MQ=255)
                                          cAAGGCCGAAGCGTTCTTTTTCAGCCAGCAGAAGCTTTAAATCGCTAACGGAATAACGCTCCTCAACCAGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTTCTCCATATTTTCTGACAAGCTCACAGGCTGTCGa                                                                                         <  8:124362/139‑1 (MQ=255)
                                           aaGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGAt                                                                                        <  6:243990/139‑1 (MQ=255)
                                                cGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTtctc                                                                                   >  2:212714/1‑139 (MQ=255)
                                                  aaGCTTTCTTTTTCAGATAGCAGCAGCTTCAAATCGCTAATAGAATAAAGATCTTCAACACGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTtctccg                                                                                 >  8:153889/1‑137 (MQ=255)
                                                            tttCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGtctc                                                                        <  2:69176/138‑1 (MQ=255)
                                                            tttCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGtctc                                                                        <  2:64290/138‑1 (MQ=255)
                                                                    agcagcagcTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGTTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTaaaa                                                               >  3:80649/1‑139 (MQ=255)
                                                                                      cTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGttt                                             >  6:309026/1‑139 (MQ=255)
                                                                                                       ctcAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGa                                                                                         <  4:15337/78‑1 (MQ=255)
                                                                                                       ctcAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGa                                                                                         >  3:15337/1‑78 (MQ=255)
                                                                                                       ctcAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTTCCGGCGATATTGCgg                            >  6:145120/1‑139 (MQ=255)
                                                                                                       ctcAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTTCg                             >  3:292149/1‑138 (MQ=255)
                                                                                                       ctcAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCg                             <  4:155089/138‑1 (MQ=255)
                                                                                                       ctcAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCg                             <  6:46650/138‑1 (MQ=255)
                                                                                                       ctcAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTGTCGGTTTTTCGGCGAGAtttggg                            >  2:332025/1‑135 (MQ=255)
                                                                                                       ctcAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCGTTCGTTTTTCCGGCGCTATTGCgg                            >  2:225926/1‑139 (MQ=255)
                                                                                                         cAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGaa                          <  5:54821/139‑1 (MQ=255)
                                                                                                          aaCACGTTCTTGATTTCCAAGAATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGTACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGaa                          <  5:261879/138‑1 (MQ=255)
                                                                                                              cGGTCTTGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAacacac                     <  3:141010/139‑1 (MQ=255)
                                                                                                              cGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGGCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAacacac                     <  5:201431/139‑1 (MQ=255)
                                                                                                                 tCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGa                  >  7:67002/1‑139 (MQ=255)
                                                                                                                   ttGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTcgca                                                                                                  <  5:62387/57‑1 (MQ=255)
                                                                                                                   ttGATTTCCAAGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTcgca                                                                                                  >  6:62387/1‑57 (MQ=255)
                                                                                                                       tttCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACg             <  3:191414/138‑1 (MQ=255)
                                                                                                                           cAAGCATTAAAACTTTGTCTTCTAATCCATATTTTCTGACAACCTCACAGGCTGTCGCGTTCACTTGACCGTCTCCCACTAAAAGCAGCTTCGCTTTCTGTTTGACCGCGATATTACGCTGCACACGGATTAAGTCCtc        >  2:91938/1‑138 (MQ=255)
                                                                                                                           cAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGTACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTTCCGGCGATATTGCCGAAAACACGGAAGACGTCCTg        >  7:12028/1‑139 (MQ=255)
                                                                                                                           cAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTg        >  5:273937/1‑139 (MQ=255)
                                                                                                                           cAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCCTCTCAAACAAAAATCAGCTTCGCTTTCTTTTTTCCGCCGATAATACGGAACACACGGAAGACGTCCtc        >  8:23673/1‑138 (MQ=255)
                                                                                                                            aaGCATTAAGACTTGGTCTTCCAAGCCATATTTTCTGATAAGCTCGCAGGCTGTCGATTTCTCCGGACCGTCTCCGACTAAAAGCAGCTTCGCTTTCGTTTTGCCAGCGATATTGCGGAACACACGGATGACATCCTGc       <  7:153889/139‑1 (MQ=255)
                                                                                                                             aGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGca      <  2:97905/139‑1 (MQ=255)
                                                                                                                             aGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGca      <  8:67002/139‑1 (MQ=255)
                                                                                                                                 ttAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTGGTTTTGGCGGCGATATTGCGGCACACACCGAAGTCGTCCTGcccccg  >  3:244500/1‑134 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TTCCGATACAAGGCACTCCACAAGCCATCGCCTCAAGAAGGACAAGGCCGAAGCTTTCTTTTTCAGACAGCAGCAGCTTTAAATCGCTAATGGAATAAAGCTCCTCAACACGGTCTTGATTTCCAAGCATTAAAACTTGGTCTTCTAATCCATATTTTCTGACAAGCTCACAGGCTGTCGATTTCTCTGGACCGTCTCCAACTAAAAGCAGCTTCGCTTTCGTTTTGCCGGCGATATTGCGGAACACACGGATGACGTCCTGCACACG  >  CP006881/2175160‑2175427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑