Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,708,259 C→T 5.7% G189D (GGC→GAC)  U712_13945 ← 3‑isopropylmalate dehydratase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,708,2590CT 5.7% 233.6 / 2.3 70G189D (GGC→GAC) U712_139453‑isopropylmalate dehydratase large subunit
Reads supporting (aligned to +/- strand):  ref base C (40/26);  new base T (2/2);  total (42/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GTGCAATCAAACCTGCTCTTGCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCAACTTCACTCGTCC  >  CP006881/2708122‑2708391
                                                                                                                                         |                                                                                                                                    
gTGCAATCAAACCTGCTCTTGCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGcc                                                                                                                                     >  1:238037/1‑139 (MQ=255)
   cAATCAAACCTGCTCTTGCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGAt                                                                                                                                  <  2:238037/139‑1 (MQ=255)
     aTCAAACCTGCTCTTGCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGa                                                                                                                                >  5:79008/1‑139 (MQ=255)
         aaCCTGCTCTTGCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGc                                                                                                                            <  8:288520/139‑1 (MQ=255)
               ctctTGCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGAt                                                                                                                      >  6:60456/1‑139 (MQ=255)
               ctctTGCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTATGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGAt                                                                                                                      >  1:224485/1‑139 (MQ=255)
                    tcTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGAc                                                                                                                   <  7:294118/136‑1 (MQ=255)
                    gCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGAc                                                                                                                   <  5:40071/137‑1 (MQ=255)
                        cTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCtt                                                                                                             <  1:12731/139‑1 (MQ=255)
                         tGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGCCAGCAAGGATGACATCCttt                                                                                                            >  7:122854/1‑139 (MQ=255)
                                   gACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTAcc                                                                                                   <  2:149195/138‑1 (MQ=255)
                                                  gTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTc                                                                                   >  5:231434/1‑139 (MQ=255)
                                                   tCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGAt                                                                                                                                  >  2:44663/1‑91 (MQ=255)
                                                   tCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGAt                                                                                                                                  <  1:44663/91‑1 (MQ=255)
                                                   tCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGc                                                                                                                            <  8:178220/97‑1 (MQ=255)
                                                   tCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGc                                                                                                                            >  7:178220/1‑97 (MQ=255)
                                                   tCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTcc                                                                                  >  2:115302/1‑139 (MQ=255)
                                                   tCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTc                                                                                   >  4:218764/1‑138 (MQ=255)
                                                   tCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTc                                                                                   >  3:253611/1‑138 (MQ=255)
                                                          ttCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTAcg                                                                           <  3:218764/139‑1 (MQ=255)
                                                          ttCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTAcg                                                                           <  8:194448/139‑1 (MQ=255)
                                                             atccatcGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGTCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTAcgcg                                                                         >  6:249550/1‑138 (MQ=255)
                                                             atccatcGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGTCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACgcgc                                                                        >  2:183363/1‑139 (MQ=255)
                                                             atccatcGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTAcgcg                                                                         <  4:68680/138‑1 (MQ=255)
                                                             atccatcGTCATATTACTGACTACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTAcgcg                                                                         <  2:31781/138‑1 (MQ=255)
                                                                       atatTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAgtg                                                              <  2:315677/139‑1 (MQ=255)
                                                                       atatTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAgtg                                                              >  1:248684/1‑139 (MQ=255)
                                                                       atatTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAgtg                                                              <  2:311404/139‑1 (MQ=255)
                                                                               gAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGa                                                      >  8:22504/1‑139 (MQ=255)
                                                                                aaTACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGAc                                                                                                                   >  3:138976/1‑77 (MQ=255)
                                                                                aaTACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGAc                                                                                                                   <  4:138976/77‑1 (MQ=255)
                                                                                aaTACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCACTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGAc                                                     >  6:50361/1‑139 (MQ=255)
                                                                                       ccccAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACTCGCACTTCAAGTGTTTTTGGACGctgctg                                              >  4:106915/1‑139 (MQ=255)
                                                                                       ccccAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGctgctg                                              >  8:324364/1‑139 (MQ=255)
                                                                                       ccccAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGctgctg                                              >  1:40139/1‑139 (MQ=255)
                                                                                            gtgtATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCaaa                                         <  2:231176/139‑1 (MQ=255)
                                                                                               tATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGc                                                                                                                            >  8:308990/1‑53 (MQ=255)
                                                                                               tATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGc                                                                                                                            <  7:308990/53‑1 (MQ=255)
                                                                                               tATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAgtg                                      >  4:149306/1‑139 (MQ=255)
                                                                                                  tCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCt                                   >  6:281792/1‑139 (MQ=255)
                                                                                                     aTGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCtgtg                                <  6:118225/139‑1 (MQ=255)
                                                                                                       gACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCtgtgtg                              >  5:305905/1‑139 (MQ=255)
                                                                                                         cGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTgg                             >  4:297131/1‑138 (MQ=255)
                                                                                                         cGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTgg                             >  1:219735/1‑138 (MQ=255)
                                                                                                         cGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTgg                             >  3:78147/1‑138 (MQ=255)
                                                                                                         cGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGa                            <  5:60456/139‑1 (MQ=255)
                                                                                                         cGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGa                            <  5:281792/139‑1 (MQ=255)
                                                                                                         cGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGa                            <  1:119228/139‑1 (MQ=255)
                                                                                                              ccTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGa                       >  7:81606/1‑139 (MQ=255)
                                                                                                               cTGTGCCGAATTTCACACCGTATTTGTCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGAc                      <  1:183363/139‑1 (MQ=255)
                                                                                                               cTGTGCCGAATTTCACACCGTATTTGTCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGAc                      <  5:249550/139‑1 (MQ=255)
                                                                                                               cTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGAc                      <  3:297131/139‑1 (MQ=255)
                                                                                                                  tgCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTAcc                                                                                                   >  5:328726/1‑59 (MQ=255)
                                                                                                                  tgCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTAcc                                                                                                   <  6:328726/59‑1 (MQ=255)
                                                                                                                  tgCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTAcg                                                                           >  4:205483/1‑83 (MQ=255)
                                                                                                                  tgCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTAcg                                                                           <  3:205483/83‑1 (MQ=255)
                                                                                                                  tgCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGGCAAAGTGTCTGTGTGGAAAGGACATg                   >  7:322984/1‑139 (MQ=255)
                                                                                                                  tgCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATg                   >  8:75146/1‑139 (MQ=255)
                                                                                                                  tgCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATg                   >  2:315211/1‑139 (MQ=255)
                                                                                                                  tgCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTATGTGTGTAAAGGACATg                   >  2:107446/1‑139 (MQ=255)
                                                                                                                   gCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGt                  >  6:212266/1‑139 (MQ=255)
                                                                                                                     cGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTc                <  8:322984/139‑1 (MQ=255)
                                                                                                                       aaTTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCaa              >  2:302148/1‑139 (MQ=255)
                                                                                                                       aaTTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCaa              >  1:16563/1‑139 (MQ=255)
                                                                                                                        aTTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCtgtg                                >  6:112759/1‑120 (MQ=255)
                                                                                                                        aTTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCtgtg                                <  5:112759/120‑1 (MQ=255)
                                                                                                                                 cGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCAACTTCACTCGt    >  5:19333/1‑139 (MQ=255)
                                                                                                                                 cGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCAACTTCACTCGt    >  5:167101/1‑139 (MQ=255)
                                                                                                                                  gTATTTGCCGATGACAGCAAGGAAGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCAACTTCACTCGTc   >  5:241601/1‑139 (MQ=255)
                                                                                                                                   tATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCAACTTCACTCGTcc  >  8:135401/1‑139 (MQ=255)
                                                                                                                                   tATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCAACTTCACTCGTcc  >  8:271748/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                    
GTGCAATCAAACCTGCTCTTGCTCCTGCTTCAATTGACATGTTACAAACAGTCATTCGTTCATCCATCGTCATATTTCTGAATACTTCCCCAGTGTATTCAATGACGTAGCCTGTGCCGAATTTCACACCGTATTTGCCGATGACAGCAAGGATGACATCCTTTGCCGTTACCCCTTTTTGAAGCGTTCCATCTACGCGCACTTCAAGTGTTTTTGGACGCTGCTGCCAAAGTGTCTGTGTGGAAAGGACATGTTCAACTTCACTCGTCC  >  CP006881/2708122‑2708391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑