| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 326,012 | C→A | 17.7% | I223I (ATC→ATA) | U712_01555 → | Uncharacterized protein ycgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 326,012 | 0 | C | A | 17.7% | 126.6 / 5.0 | 64 | I223I (ATC→ATA) | U712_01555 | Uncharacterized protein ycgA |
| Reads supporting (aligned to +/- strand): ref base C (26/25); new base A (8/5); total (34/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGATTTAACTCCACCATATTATCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAGCTGA > CP006881/325875‑326148 | gcgggATTTAACTCCACCATATTATCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCt < 1:156857/136‑1 (MQ=255) tttttAACTCCACCATATTATCACCGTCACCGCTCGGTTTATCACAATCTATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTAt < 6:118740/135‑1 (MQ=255) gATTTAACTCCACCATATTATCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATTCATATGCTTTTTGCTGTCTTCTATTATTTATATATAttt > 5:118740/1‑139 (MQ=255) aTTTAACTCCAACATATTATCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTg < 1:268074/139‑1 (MQ=255) tAACTCCACCATATTATCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGta < 4:48705/138‑1 (MQ=255) aaCTCCACCATATTATCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGtat > 7:111245/1‑138 (MQ=255) caccaTATTATCACCGTCACCGCTCGGTTTATCACAATCAATCGCGCAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGATGAGATATACATAAGCTTTTTGCTGTCTTCTATTATTTATATATATTTTTATAagag > 2:268074/1‑138 (MQ=255) tattatCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCGCGTTTCTCAGGACTCGGCCTGCGAGTGGTGAGATACATATGCTTTTTGCTGTCTTCTATTATTTATAGATATTTGTTAACGAGAAAAt > 2:335323/1‑138 (MQ=255) atCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTaaa < 1:10314/138‑1 (MQ=255) accgtcaccgCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTTTTTATTTATATTTTTTTAAGAGAAAAATaaaaaa > 1:145713/1‑138 (MQ=255) ccgtcaccgCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAGTCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGATAAAATTaaaaaaa > 2:269970/1‑138 (MQ=255) ccgtcaccgCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTaaaaaaa > 2:74479/1‑138 (MQ=255) ccgtcaccgCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATATATTTTTATACGAGAAAATTaaaaaaa > 4:90592/1‑138 (MQ=255) tcaccgCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATATATTTGTATACGAGAAAATTAAAAAAATCa > 2:185516/1‑138 (MQ=255) cgCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTGTATTATTTATATCTATTTGTATAAGAGAAAATTAAAAAAATAaaaata > 8:168651/1‑137 (MQ=255) cgCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCAGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCaa > 5:125594/1‑135 (MQ=255) gttttATCAAAATCAAACTCGGAGATCCCTCTTTTATCAGCAATCGTCCTGCGAGTTGTGATATATATATGCTTTTTGGTGTCTTGTATTATTTATATATATTTGTAAACGACAAAATTAAAAAAATCAAAAGATGcc < 6:77979/136‑1 (MQ=255) ggTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGTAATCGGCCTGCTAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAATAGATGcc < 1:74479/138‑1 (MQ=255) ggTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGcc < 6:273283/138‑1 (MQ=255) tttATCACAATCAATCGCGGAGCTCCCTATTTTCTCAGGACTCGGCCTGCGAGTTGTTATAAACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGACAAAATTAAAAAAATCAAAAGATGCCAgt < 5:318557/139‑1 (MQ=255) tttATCAAAATCACTCGCGGAGGTCGCTCTTTTATCAGGAATCGGCCTGCGAGTTTTGATAAACATATGCTTTTTGCTGTGTTCTATTATATATATATATTTGAATACGTCAAAATAAAAAAAATCAAAAGATGCCAgt < 4:198858/139‑1 (MQ=255) ttATCACAATCAATCGCGGAGCTCCCTCTTTTCTCATGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAgt < 7:168651/138‑1 (MQ=255) caatcaatCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAg < 5:54151/139‑1 (MQ=255) tcaatcGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGaa > 1:88421/1‑139 (MQ=255) tcaatcGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGaa < 6:125594/139‑1 (MQ=255) tcaatcGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGaa > 7:59845/1‑139 (MQ=255) tcGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGtt < 6:235016/130‑1 (MQ=255) tcGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGtt > 5:235016/1‑130 (MQ=255) tcGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGaa > 6:56327/1‑135 (MQ=255) cgcgGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTTTTATTTATATCTATTTGTTTACGAGAAAATTAAAAAAATCAAACGATGCCAGTGTGTTAGGAACAGAt < 3:90592/139‑1 (MQ=255) aGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGtt < 2:88421/139‑1 (MQ=255) cTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATAGGCTTTTTGCTGTCTTGGATTTTTTTATTATATTTGTATACGAGAAAATTAAATAAATATAAAGATGCCATTGTGTTAGGAACAGAGTGGTTcc > 8:127698/1‑139 (MQ=255) tctcAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTTTGTTAGGAACAGATTGGTTCCCTGCGGCAg > 1:28601/1‑138 (MQ=255) tCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTTTTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAATGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGa < 3:234064/139‑1 (MQ=255) tccTGCGTGTTGTGATATACATATGCTTTTTGCTGTCTTATATTATTTATATATATTTGTATACGAGAAAATTAAAAAAATAAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGc < 6:135995/138‑1 (MQ=255) cGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGt > 5:135995/1‑138 (MQ=255) ttgATATAAATATGCTTTTTGCTGTCTTCTATTATTTATATATATTTTTATACTATAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAaagaa < 8:127703/137‑1 (MQ=255) gtgATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAaagaa < 8:59845/138‑1 (MQ=255) tgATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAaagaag < 1:185516/138‑1 (MQ=255) tataCATATGCTTTTTGCTGTCTTAAATTATTATTATCTATTTTAATACGATAAAAAAAAAAAAAACAAACGATGCCAGTGTGTTAGGAACTGATTGGTTCCCTGCGGCCGGAATGGGCGAAGCCGGTAAagaagaaga < 2:145713/139‑1 (MQ=255) atGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACTAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAaagaa < 5:248971/127‑1 (MQ=255) atGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAaagaa > 6:248971/1‑127 (MQ=255) atGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGAtcagtcc > 7:127703/1‑138 (MQ=255) atGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGAtcagt > 3:322046/1‑137 (MQ=255) atGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGAtcagt > 1:163811/1‑137 (MQ=255) atGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGAtcagt < 4:322046/137‑1 (MQ=255) tGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAAGAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGAtcagtca < 7:203762/138‑1 (MQ=255) gCTTTTTGCTGTATTATATTATTTATATATATTTGTATACGAGAAAATTAAAAAAATCAAATGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATcagtcagt < 8:35094/139‑1 (MQ=255) ttttGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGcc > 2:140749/1‑139 (MQ=255) tGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGtt > 1:87805/1‑139 (MQ=255) tGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGtt > 7:194495/1‑139 (MQ=255) tGCTGTCTTCTATTATTTATATATATTTGTATAATAGAAAATTAAAAAAATCAAAAGATGCCAGTTTGTTAGTAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGACGGTAAATAAGAATATAAGTCCGTGCCGtt > 3:35262/1‑139 (MQ=255) tcttctATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCg > 7:35094/1‑139 (MQ=255) tcttctATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCg > 7:47645/1‑139 (MQ=255) cttctATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGt > 2:283193/1‑139 (MQ=255) ctATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGt < 8:111245/136‑1 (MQ=255) tataTCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAg < 1:269970/138‑1 (MQ=255) tataTCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAGc > 8:153072/1‑139 (MQ=255) tataTCTATTTGTAAACGACAAAAATAAAAAAAACAAAAGATGCCAGTGTGCTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTGCGCCATAAg < 3:241953/138‑1 (MQ=255) atCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAg > 6:227748/1‑135 (MQ=255) atCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAg < 5:227748/135‑1 (MQ=255) atCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAGCTGa > 2:84560/1‑139 (MQ=255) atCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAGCTGa > 7:10923/1‑139 (MQ=255) atCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAGCTGa > 3:58372/1‑139 (MQ=255) | GCTGGATTTAACTCCACCATATTATCACCGTCACCGCTCGGTTTATCACAATCAATCGCGGAGCTCCCTCTTTTCTCAGGAATCGGCCTGCGAGTTGTGATATACATATGCTTTTTGCTGTCTTCTATTATTTATATCTATTTGTATACGAGAAAATTAAAAAAATCAAAAGATGCCAGTGTGTTAGGAACAGATTGGTTCCCTGCGGCAGGAATGGGCGAAGCCGGTAAAGAAGAAGATCAGTCAGTGCCGTTTACCGTTCGCCATAAGCTGA > CP006881/325875‑326148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |