| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,348,914 | C→G | 26.8% | G142G (GGC→GGG) | U712_17210 → | Para‑nitrobenzyl esterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,348,914 | 0 | C | G | 26.8% | 69.6 / 4.3 | 35 | G142G (GGC→GGG) | U712_17210 | Para‑nitrobenzyl esterase |
| Reads supporting (aligned to +/- strand): ref base C (4/20); new base G (5/2); total (9/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGTGTGGATTCACGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAGGCGTATTCTGATAACCTTGGGCTTTTAGACCAAGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGATAACGTAAC > CP006881/3348786‑3349039 | aTGGTGTGGATTCACGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGGCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCAc < 5:260457/138‑1 (MQ=255) gtgtGGATTCACGGAGGCGCGTTTTTTGTAGGAGCGGGCAGTGAGCCCTTTTATTGCGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGGTACAATGAACAATAGGCTGGGGGCGG‑TTTGGGGTTTTgtcgttt > 4:243667/1‑133 (MQ=255) tgtgGATTCCCGGCGGCGCTTTTTAGCTAGTGCCGGCCCGTGAGCCATTGTATGACGCAGCAAACCTGGCCGCCCAGGCAGAAGTAATTGTCGTAACATTCACCTACCGCCG‑GGGGCCG‑TTTGGCTTTTTCCACTTggc < 8:253141/139‑3 (MQ=255) tgtgGATTCACGGAGTCGCTTTTTATCTAGGAGCTGGCAGTTAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCTTTGTCGTTTCATTGAACTATCGGCT‑GGGTCCG‑TTTGGCTTTTTGCACTTGTc < 1:44640/139‑1 (MQ=255) cGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAt < 7:250724/139‑1 (MQ=255) cGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAt < 3:187372/139‑1 (MQ=255) cGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAt < 8:192014/139‑1 (MQ=255) cGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAt < 3:64508/139‑1 (MQ=255) cGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAt < 3:73386/139‑1 (MQ=255) cGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAt < 7:330012/139‑1 (MQ=255) aGGCGCTTTTTATCTAGCAGCGGGCAGGGAGCCATTGTATGCCGGAGCAAACCTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATATAAT‑GAg < 2:266309/139‑1 (MQ=255) ggtgcTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAgg < 7:48496/136‑1 (MQ=255) cTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAGGCGTa < 7:155613/139‑1 (MQ=255) cTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAGGCGTa < 5:218729/139‑1 (MQ=255) cTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCG‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAGGCGTa < 7:99586/139‑1 (MQ=255) tCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAGGCGTATTCTGa < 3:77320/138‑1 (MQ=255) ggAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTTGACTTGTCTTGATTTAAT‑TAGGCGTATTCTGATAAc > 7:38916/1‑138 (MQ=255) ggAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGAACTTGTCTTCATTTAAT‑GAGGCGTATTCTGAt < 7:102903/135‑1 (MQ=255) ggCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TGTGGGGTTTTGGACGTGTGTTGATTTTAT‑TAGGGGGATTGTGGTTACCGTgggg > 8:330990/1‑138 (MQ=255) tGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCGTTTTGGGTTTTTG‑ACATTTATTAATTTAAT‑TATGCGGATTATTATAAACATGGGCGTTTATACCaaac > 5:121146/1‑137 (MQ=255) gACGGATAAAAAGTTGGGGCACAGGGAGATTTCTTTGTCGTTACTTTGAACTATCGGCG‑GGGGCGG‑TTTGGATTTTTGCACTTTTCTTCATTTAAT‑GAGGCTTATTCTTATAACCTTGGGCTTTTAGACCAAgccgcc < 8:63185/138‑1 (MQ=255) aCGGATCAAAACTTGCGGCCCAGGGATAAGTCTTTGTCGTTACATTTAACTATCGGCT‑GGGGCGG‑TTTGGGTTTTTGCACTTGTCTTCATTTAAT‑GCGGCGTATTCTGATAACCTTGGGCTTTTACACCAAGCcgccgc < 8:38916/139‑1 (MQ=255) cAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTTGCGTTTTGCACATTAGATAATTTTAT‑TAGGCGTATTATGATAACCATGGGCTTTTAGAACAAACCGCCCCGCGGa > 8:167927/1‑138 (MQ=255) gCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAGGCGTATTCTGATAACCTTGGGCTTTTAGACCAAGCCGCCGCGCTGAAATGGGTGc > 5:134836/1‑139 (MQ=255) gCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TGTGGGTTTTTGCCCTTGTCTTCATTTAAT‑GCGGGGTATTATGATAACCTTGGGCGTTTAGACCAACCCGCCCCGCGGAAATGGGTGc > 7:112467/1‑139 (MQ=255) ggCACAGGGAGATGTCTTTGTGTTTACATTTAACTATGGGGG‑GGGGCGG‑TTTGGCTTTTTGCACTTGTTTTAATTTAAT‑GCGGGTTATTGTTAAAACGTTGGGCTTTTACACCAAGCCGCCGCGCTGAAATGGGTGCga < 4:154407/139‑1 (MQ=255) tCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGGGGTTTGGGCGTGTGGTGATTTTAT‑GAGGGGGTTTGGGGTAAACTTGGGGGTTTTTGCCCAGCCGCCGCGCGGCAATGGGGGGGTGAGGATATTTTAGc > 6:75939/1‑139 (MQ=255) tttGAACTTTGGGCT‑GGGGCCG‑TTTGGTTTTTTGCACTTGTCTTAATTTATT‑GTGGCTTTTTATGATAACCTTGGGCTTTTAGACCAAGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGAt < 6:121146/138‑1 (MQ=255) acaccGGCC‑GGGGCCC‑TTTTGGTTTTTTCAATTTGTTTAATTTAATAGCGGGTTTTTCTTAAAACGTTGGGTTTTTACAAAACGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGa < 3:11618/135‑1 (MQ=255) cT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTATTCATTTATT‑GAGGGGTATTCTGATAACCTTGGGGTTTTAGACCAAGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGATAACGTaa < 7:167927/139‑1 (MQ=255) t‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GTGGCGTTTTCTGATAACCTTGGGCTTTAACACCAAGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGATAACGTAAc < 7:171929/139‑1 (MQ=255) t‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GCGGCGTATTCTGATAACCTTGGGCTTTTAGACCAAGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGATAACGTAAc < 7:253760/139‑1 (MQ=255) t‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAGGCGTATTCTGATAACCTTGGGCTTTTAGACCAAGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGATAACGTaa > 3:165164/1‑138 (MQ=255) tgggcgcG‑TTTGGTTTTTTGCAGTGGTCTTCATTTAAT‑GCGGCGTTTTCTGATACCCTGGGGCTTTTACACAACGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGATAACGTAAc < 3:291421/133‑1 (MQ=255) gggggCGG‑TTTGGTTTTTTCCTCTTGATTTATTTTATT‑GCGGCTTATTGTGAAACCGTGGGGCTTTTCCACCAAGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGATAACGTAAc < 1:151627/138‑1 (MQ=255) | ATGGTGTGGATTCACGGAGGCGCTTTTTATCTAGGAGCGGGCAGTGAGCCATTGTATGACGGATCAAAACTTGCGGCACAGGGAGAAGTCATTGTCGTTACATTGAACTATCGGCT‑GGGGCCG‑TTTGGCTTTTTGCACTTGTCTTCATTTAAT‑GAGGCGTATTCTGATAACCTTGGGCTTTTAGACCAAGCCGCCGCGCTGAAATGGGTGCGAGAGAATATTTCAGCGTTTGGCGGTGATCCCGATAACGTAAC > CP006881/3348786‑3349039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |