Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,354,774	A→C	9.4%	K303N (AAA→AAC)	U712_17245 →	Levansucrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,354,774	0	A	C	9.4%	117.7 / 2.3	51	K303N (AAA→AAC)	U712_17245	Levansucrase
Reads supporting (aligned to +/- strand):  ref base A (20/26);  new base C (2/3);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

GAAGCAAACACTGGAACTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGTAACAGATGAAATTGAACGCGCGAAC  >  CP006881/3354649‑3354900                                                                                                                              |                                                                                                                               gAAGCAAACACTGGAACTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTAAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTTAg                                                                                                                    <  8:225976/138‑1 (MQ=255)  aaGCAACCACAGGAACTGAAGATCGCTACCAAGGCGAAGAAACTTTATTTAACAAATCATACTATGGCAAAACAACATCATACTTCCGACATGAAAGTCAAAAAATACTGCATCTAGATAAAAAACGCACGGCTGAGt                                                                                                                   >  2:170753/1‑138 (MQ=255)      aaaCACTGGAACTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCTCATCATTCTTACGTCATGAAAGTCAAAAACTTCTGCAAAGCGATAAAAACCGCACGGCTGAGTTAGc                                                                                                               >  6:186392/1‑138 (MQ=255)      aaaCACTGGAACTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAg                                                                                                                    <  1:70639/133‑1 (MQ=255)      aaaCACTGGAACTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAg                                                                                                                    >  2:70639/1‑133 (MQ=255)      aaaCACTGGAACTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGc                                                                                                               >  4:77761/1‑138 (MQ=255)      aaaCACTGGAACTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGc                                                                                                               >  8:104344/1‑138 (MQ=255)       aaCACTGGAACTGAAGATGGCTACCAAGGCTAATAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCaa                                                                                                             <  5:323980/139‑1 (MQ=255)              gAACTGAAGATGGCTACCAAGGCGAAGAATCTTAATTTACCAACCCATCCTATGGAAAACGAACATCATTCTACCGGCAAGAAAGGCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGgcg                                                                                                       <  8:3722/138‑1 (MQ=255)               aaCTTAAGAGGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGAAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGct                                                                                                     <  7:324808/139‑1 (MQ=255)               aaCTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCATAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGct                                                                                                     <  7:331481/139‑1 (MQ=255)               aaCTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCAAACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGct                                                                                                     <  7:104344/139‑1 (MQ=255)               aaCTGAAGATGGCTACCAAGGCGAAGAATCTTTAATAAACAAAGCATACTATGGCAAACGCACATCATTCTTCCGCCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGct                                                                                                     <  7:68111/139‑1 (MQ=255)                    aaGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGCCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCgg                                                                                                <  7:223106/139‑1 (MQ=255)                     aGATGGCTACAAAGGCTAACAATCTTTATTTAACAAAGCATACTTTGGAAAAACCACATCATTATTCGTTCAAGAAAGCAAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCgg                                                                                                <  2:259561/138‑1 (MQ=255)                          gCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACAGCTGAGTTAGCAAACGGCGCTCTCGGTATGAt                                                                                          >  2:242483/1‑139 (MQ=255)                              ccAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAg                                                                                      >  4:142165/1‑139 (MQ=255)                                aaGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACg                                                                                                                              >  7:231506/1‑97 (MQ=255)                                aaGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACg                                                                                                                              <  8:231506/97‑1 (MQ=255)                                 aGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTa                                                                                   <  3:108005/139‑1 (MQ=255)                                    cgtataaTTTTTATTTAAAAAAGAATAGTTTGGCAAAAGCACATAATTCTTACTTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAACCGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAAc                                                                                <  8:324247/134‑1 (MQ=255)                                    cGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAAc                                                                                <  3:142165/139‑1 (MQ=255)                                      aataaTCTTTATTAAACAAAGCATACTATGGCAAAAGCACAACATTCTACCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACga                                                                              <  5:187725/136‑1 (MQ=255)                                               tatttaACAAAGCATAATATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTa                                                                        <  8:259615/136‑1 (MQ=255)                                                         aGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAAc                                                                                <  1:233821/118‑1 (MQ=255)                                                         aGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAAc                                                                                >  2:233821/1‑118 (MQ=255)                                                         aGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGaaaa                                                             >  3:277931/1‑137 (MQ=255)                                                                   ggCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAACCGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAAc                                                 >  2:121478/1‑139 (MQ=255)                                                                     cAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGTTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCg                                               >  6:9613/1‑139 (MQ=255)                                                                     cAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCg                                               >  4:172011/1‑139 (MQ=255)                                                                         accacaTCAGTCTTCCGTCAACAAAGTCAACAACTGCTGCAAAGCGATAAAAACCGCACGGCTGAGGTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAACTGATGAAACCGCTGa                                           <  7:155756/137‑1 (MQ=255)                                                                         aGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACTGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGa                                           <  1:242483/139‑1 (MQ=255)                                                                          gCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGAt                                          >  6:160998/1‑139 (MQ=255)                                                                           cacaTCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGAtt                                         <  5:160998/139‑1 (MQ=255)                                                                           cacaTCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGAtt                                         <  4:277931/139‑1 (MQ=255)                                                                           aacaTCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGAtt                                         <  3:70411/138‑1 (MQ=255)                                                                             catcatTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGAGTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGACACCGCTGATTg                                        >  5:288786/1‑138 (MQ=255)                                                                                   tcttcCGTCAAGAAAGTCAAAAACTTCTGAAAAGCTATAAAAAACGCAAGGCTGAGTTAGAAAACGGCGCTCTCGGTATTATTGTGCTACACGATGATTACACACTGAAAAAACTGATGAAACCGCTGATTGCATCt                                   <  7:269842/137‑1 (MQ=255)                                                                                       ccGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAaca                               >  6:51951/1‑137 (MQ=255)                                                                                          tCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAacacctgt                          >  5:182957/1‑135 (MQ=255)                                                                                          tCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGt                           >  4:116709/1‑138 (MQ=255)                                                                                          tCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGt                           >  5:61022/1‑138 (MQ=255)                                                                                            aacaaaGTCAAACTATTCTTCAATGCTATAAAAAACGCAAGGCTGAGTTAGCAAACGGCGCTCTCTGTATGATTGAGCTAAACTATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGTAAc                        <  1:270155/136‑1 (MQ=255)                                                                                                aaGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGTAACAGAt                    <  3:77761/139‑1 (MQ=255)                                                                                                             cTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGAtt                                         >  7:146520/1‑105 (MQ=255)                                                                                                             cTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGAtt                                         <  8:146520/105‑1 (MQ=255)                                                                                                             cTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGTAACAGATGAAATTGAAcgcg       >  6:3171/1‑139 (MQ=255)                                                                                                             cTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGAATGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGAtt                                         <  2:281890/105‑1 (MQ=255)                                                                                                             cTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGAATGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGAtt                                         >  1:281890/1‑105 (MQ=255)                                                                                                                 aaaGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGTAACAGATGAAATTGAACGCGCGaa   <  7:159913/139‑1 (MQ=255)                                                                                                                  aaGCGATAAAAACCGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGTAACAGATGAAATTGAACGCGCGAAc  <  1:121478/139‑1 (MQ=255)                                                                                                                              |                                                                                                                               GAAGCAAACACTGGAACTGAAGATGGCTACCAAGGCGAAGAATCTTTATTTAACAAAGCATACTATGGCAAAAGCACATCATTCTTCCGTCAAGAAAGTCAAAAACTTCTGCAAAGCGATAAAAAACGCACGGCTGAGTTAGCAAACGGCGCTCTCGGTATGATTGAGCTAAACGATGATTACACACTGAAAAAAGTGATGAAACCGCTGATTGCATCTAACACAGTAACAGATGAAATTGAACGCGCGAAC  >  CP006881/3354649‑3354900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑