Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,476,682 G→T 32.0% T22K (ACA→AAA)  U712_17855 ← putative undecaprenyl‑phosphate galactose phosphotransferase‑like Protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,476,6820GT32.0% 34.3 / 5.6 41T22K (ACA→AAA) U712_17855putative undecaprenyl‑phosphate galactose phosphotransferase‑like Protein
Reads supporting (aligned to +/- strand):  ref base G (13/13);  new base T (7/6);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAG  >  CP006881/3476558‑3476804
                                                                                                                            |                                                                                                                           
ctcCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCGTTTGAGCTAATTTTAAAAAATGTAtt                                                                                                               >  4:49491/1‑139 (MQ=255)
 tcCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGCTAATGTAAAAGAATGTAttt                                                                                                              >  5:74340/1‑139 (MQ=255)
 tcCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTAttt                                                                                                              >  5:295131/1‑139 (MQ=255)
 tcCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCGTTTCAGATAATTTAAAAGAAAGTAttt                                                                                                              >  5:56758/1‑139 (MQ=255)
 tcCAAACAAATCAAAATTGAAAATAACGCAATAATTGGCAGCGCAATAGCCAATCCAATGAGCGCAAACCAAATATACATTACTCGCTTTATTGGAAAATAACGCGCCGTGTGTTTAGTTAATTTTAAATATAGTAttt                                                                                                              >  4:22230/1‑139 (MQ=255)
          aTCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATACCGAGCCGTCGTTTCAGTTAATGTAAAAGTATGTATTTGTTGTACTg                                                                                                     >  2:318398/1‑139 (MQ=255)
          aTCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACt                                                                                                      >  1:136489/1‑138 (MQ=255)
          aTCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACt                                                                                                      <  2:136489/138‑1 (MQ=255)
          aTCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTAAGATAATGTAAAAGAATGTATTTTTTGAACTg                                                                                                     >  1:64605/1‑139 (MQ=255)
           tcaaaattcaaaatACCGCAATCATTGGCAGCGCAATCGCCAAACCAATGAGCGCAAACAAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATTATGTAAAACAATGTATTTGTTGAACtta                                                                                                    <  4:205540/139‑3 (MQ=255)
           tCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTGAGATAATTTAAAAGAATGTATTTGTTTaagt                                                                                                      >  1:262087/1‑135 (MQ=255)
           tCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTga                                                                                                    <  5:158795/139‑1 (MQ=255)
                       aacccGCAATCATGGCCAGCGCAATCGCCAAACCAATGCGCGCAACCCAAATAACCATACCGCGCTTTATTCCCAAAAAACGCCCCGTCTTTGCAGAAAATGTAAAACAATGTATTTGTTGAACTGAGAATGCACGACt                                                                                        <  7:104164/136‑1 (MQ=255)
                                   ttGGCCGCCCAATCGCCAAACCAATGAGCGCAAACCAAATATCCATTCCGCGTTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGAAAAAGAATGTATTTGTTCAACTGAGAATTCACGACTCACATTCATGCt                                                                            <  5:144296/139‑1 (MQ=255)
                                   tgggCCGCCCAATCGCCAAACCAATGAGCGAAAACCAAAAAACAATTACGCGATTTATTCCAAAATAACGCCCCGTCTTTTCAGAAAATGAAAAACAATGTATTTGTTCAACTGACAATACACGACTCACATTCATGCt                                                                            <  8:314052/137‑1 (MQ=255)
                                    tgccAGCCCAAACGAAAATCAAATCAGCGAAAACAAAATAACCATTCCCCTATTTATTCAAAAAAAACGAGCCGTCTTTTCATATAATTAAAAATATTTTTTTTGTTCAACTTACAATCCACGACTCACATTCATGCt                                                                            <  6:82960/135‑1 (MQ=255)
                                     ggCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATttg                                                                                                             <  4:292228/104‑1 (MQ=255)
                                     ggCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATttg                                                                                                             >  3:292228/1‑104 (MQ=255)
                                          cgcAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTGTTTTAAGATAATGTAAAAGAATTTATTTGTTGAACTGAGAATTAACGACGCACATTCATGCTTTTatct                                                                     >  5:82960/1‑139 (MQ=255)
                                          cgcAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATTTAAAATAATGTTTTTGTTTAACTGGGAATTAACGACTCCCCTTAATGCttttgtgt                                                                     >  3:81995/1‑135 (MQ=255)
                                            cAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATttgtt                                                                                                           >  4:192551/1‑99 (MQ=255)
                                            cAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATttgtt                                                                                                           <  3:192551/99‑1 (MQ=255)
                                             aagcGCCAATCAAATGAGCCCAAACCAAAAATCCATTCCGCGCTTTATTGCCAAATAACGCGCCGTCTTTACATATAATTAAAAAGATTGTTTTTGTTCAACTGATAATCCACGACTCACATTCATGCTTTTCTCTGca                                                                  <  6:56758/136‑1 (MQ=255)
                                             aacccccaaaccaatCCCCGCAAAAAAAAAATCAATCCCCCTCTTTATTCCCAAAAACCGCGCCGTCTTTTCATAAAATCTAACACATTTTATTTTTTAAACTGACAAACCACGACTCACATTCATGCTTTTCTCTGca                                                                  <  2:262087/134‑1 (MQ=255)
                                             aaTCCCCAATCAAATGAGCGAAAACAAAATATCAATTCCGCGTTTTATTGCAAAAAACCGAGCCGTATTTTCATAAAATTAAAAATATTTTTTTTGTTTAACTGACAATCCACGACTCACATTCATGCTTTTCTCTGca                                                                  <  6:295131/139‑1 (MQ=255)
                                                      tccaatGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTGGTTTAAGATAATATAAAACAATTTATTTGTTGAACTGAGAATTCAAGAATCACATTAATGCTTTTCTATGaaatcacaggc                                                         >  1:197096/1‑135 (MQ=255)
                                                      tccaatGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCGTTTCAGATAATTTAAAAGAATTTATTTGTTGTAATGTGAATTAACGCCTCCCAGTCTTGCTTTTGTCTGGACTCAC‑TGac                                                        >  3:17948/1‑139 (MQ=255)
                                                           tGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCGTTTCAGATAATGTTAAAGAATTTATTTGTTTTACAGATAATTGAAGACTCACATTCATGGATTTATCATCACACAC‑AGacacacc                                                   >  2:250074/1‑138 (MQ=255)
                                                             aGCGAAACCCAAATATCCATTACGCGATTTATTCCAAAATAACGAGCCGTCTTTTCATAAAATGTAAAATAATGTATTGGTTGAACTGACAATCCACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGacacacac                                                  <  2:17648/138‑1 (MQ=255)
                                                              gccaaaaCCAATAAACCATTGCGCGCTTTATTGCAAAAAAACGAGCCTTATTTTAAGATAATGTAAAAGATTTTTTTTTTTGCAGTGACAATACACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCt                                                <  1:54700/135‑1 (MQ=255)
                                                                    aCCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAATAATGTATTTTTTGAACTGAGAATTCACGACTCACATTGAAGCTTTTCTGTGCACTCAC‑TGACAAACACCGCaacat                                          >  8:64911/1‑135 (MQ=255)
                                                                    aCCAAATAACCATTACGCGCTTTATTGCCAAAAAACCCGCCGACTTTACACAAAATGTAAAAGAATTTATTTGTTCAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGAt                                          <  4:222846/139‑1 (MQ=255)
                                                                                ttACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAAATTAAAATAATTTATTTGTTTTACTGAGAATTAACGACACACAATCATGCTTTTATCTG‑ACAGACATCACACACACCGACCCATTTGTTTGGGTTg                              >  1:129752/1‑139 (MQ=255)
                                                                                   cgcgTTTTATTGCAAAATAAGGTGCGGTTTTTACATATATTTAAAAATTTTTTTTTTTTTTAATTTAAAATCCACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGtt                            <  1:250074/138‑1 (MQ=255)
                                                                                     cgcTTTATTGCCAAATAACGAGCCGTCGTTTAAGATAATGTAAAAGAATGTATTTGTTGAACTGATAATTAACGACTCACATTAATGCGTTTCTCTGCACTCAA‑AGACACACACCTCACGATTTGTTTGGGATGTTttg                         >  2:22861/1‑139 (MQ=255)
                                                                                      gcTTTATTGCAAAATAACGCGCCGTATTTTCATATAATTTAACATTATGTATTTGTTTAACTGACAATACACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGTTatga                        <  7:64911/139‑1 (MQ=255)
                                                                                          tATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTTAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGTTTTGATTAc                    >  3:124310/1‑139 (MQ=255)
                                                                                           atctccATATAACGAGCCGTATTTTCATATAATGAAAAATATTTTTTTTTTTTAAATCATAATTCACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGTTATGATGAc                    <  7:219166/134‑1 (MQ=255)
                                                                                            ttGCCAAATAACGAGCCGTCTTTTCCTAAAATGAAAAAGAATTTATTTTTTCAACTGAGAATCCACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGTTATGATGAcc                   <  3:82645/138‑1 (MQ=255)
                                                                                                  aaTAACGCGCCGTCTTTTCATATAATTTAAACGAATGTATTTTTTTAACGGACAACACACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCca            <  4:81995/139‑1 (MQ=255)
                                                                                                              tCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTAATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAg  >  1:176122/1‑137 (MQ=255)
                                                                                                                            |                                                                                                                           
CTCCAAACAAATCAAAATTGAAAATACCGCAATCATTGGCAGCGCAATCGCCAATCCAATGAGCGCAAACCAAATATCCATTACGCGCTTTATTGCCAAATAACGAGCCGTCTTTTCAGATAATGTAAAAGAATGTATTTGTTGAACTGAGAATTCACGACTCACATTCATGCTTTTCTCTGCACTCAC‑TGACACACACCTCACGATTTGTTTGGGATGTTATGATGACCTATGCCAGTCAGTTTAG  >  CP006881/3476558‑3476804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑