Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,579,791	(A)7→6	coding (79/132 nt)	U712_18380 →	Hypothetical Protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,579,785	0	A	.	97.6%	360.1 / 0.8	83	coding (73/132 nt)	U712_18380	Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base . (53/28);  total (55/28)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTT  >  CP006881/3579649‑3579915                                                                                                                                         |                                                                                                                                   gAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaa                                                                                                                                   >  1:141228/1‑138 (MQ=255)    gcaagcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCGATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaa                                                                                                                                   >  4:220099/1‑135 (MQ=255)     aaatcaTACAATAGACATATCTAGTAGAGGAAATAAACAATGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATGCTAAACCGCAGTTTTTTTTTCGGGTGTCTGCATGCaaa                                                                                                                                  <  4:261844/131‑1 (MQ=255)      aagcaTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaaca                                                                                                                             <  8:23332/139‑3 (MQ=255)       agcaTACAATAGACATCTCGAGTAGAGGAATTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAATAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTAGTTCGGGTGTCTGCATGCaaaaaacac                                                                                                                            >  2:29175/1‑136 (MQ=255)          aTACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCaaaaaaca                                                                                                                             >  6:144163/1‑133 (MQ=255)                   aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATc                                                                                                                  >  3:314181/1‑136 (MQ=255)                   aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                               >  1:224783/1‑139 (MQ=255)                   aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                               >  7:181973/1‑139 (MQ=255)                   aCATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                               >  2:263392/1‑139 (MQ=255)                     aTCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTg                                                                                                             >  8:383702/1‑139 (MQ=255)                      tctcTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                               >  5:328830/1‑136 (MQ=255)                      tctcTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                               >  5:175184/1‑136 (MQ=255)                      tctcTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                               >  6:174120/1‑136 (MQ=255)                      tctcTAGTAGAGGAAATCAACAAAGCAACGATGTTACCAAGAAAAGAATATCAAAAAAACTGATGAAGTCGTAATCAATCAGAAACCGCACTTTTTATGTCGGGTGTCTGCATG‑AAAAAAACACACCATGATCGTc                                                                                                               >  3:238498/1‑136 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                           >  2:900/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                           >  2:96781/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                           >  1:102030/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                           >  7:44036/1‑138 (MQ=255)                        tctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                           >  8:217852/1‑138 (MQ=255)                         ctAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTg                                                                                                             >  5:261120/1‑135 (MQ=255)                          tagtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGACACCGCACGTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTgg                                                                                                            >  4:123541/1‑135 (MQ=255)                          tagtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTc                                                                                                               <  6:175184/132‑1 (MQ=255)                          tagtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACACGGATCGTCTgg                                                                                                            >  5:428505/1‑135 (MQ=255)                           agtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                           >  3:36705/1‑135 (MQ=255)                           agtagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                           >  3:352313/1‑135 (MQ=255)                           agtagAGGAAACCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGa                                                                                                           >  5:43317/1‑135 (MQ=255)                             tagAGGTAATCAACAAAGCACCGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTGTATTTCGGGTGTCTGCAGGC‑AAAAAACACTCCCGGATCGTCTGGaaaa                                                                                                        >  4:255014/1‑136 (MQ=255)                             tagAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGaaaa                                                                                                        >  6:179008/1‑136 (MQ=255)                               gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtctc                                                                                                    <  1:138208/138‑1 (MQ=255)                               gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAtctc                                                                                                    >  2:85871/1‑138 (MQ=255)                               gagGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGGCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAAt                                                                                                       <  5:286929/135‑1 (MQ=255)                                 ggAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                 <  7:217852/139‑1 (MQ=255)                                     aTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                 >  6:202304/1‑135 (MQ=255)                                     aTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                 >  3:254638/1‑135 (MQ=255)                                     aTCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaac                                                                                                 >  3:432734/1‑135 (MQ=255)                                      tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaacaa                                                                                               <  6:380472/136‑1 (MQ=255)                                      tCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaacaa                                                                                               <  4:36705/136‑1 (MQ=255)                                      tCAACAAACCAACGCTGTTACCCAGAAATTAATATCAAAAAAACGGAGTAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTGGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTcaacaa                                                                                               <  3:40449/136‑1 (MQ=255)                                       caactaAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAAt                                                                                              >  3:240788/1‑136 (MQ=255)                                       caacaaAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAAt                                                                                              >  3:250211/1‑136 (MQ=255)                                         acaaAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCggg                                                                                         <  2:57570/139‑1 (MQ=255)                                           aaaGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTg                                                                                       <  2:353844/139‑1 (MQ=255)                                                 aCGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAg                                                                                    <  4:250211/136‑1 (MQ=255)                                                  cGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtct                                                                                >  2:314201/1‑139 (MQ=255)                                                  cGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAtct                                                                                >  7:305606/1‑139 (MQ=255)                                                     tGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAACATCTCAACAATCCGGGTGCAGAtct                                                                                >  6:389216/1‑136 (MQ=255)                                                         aCCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTa                                                                          <  7:346856/138‑1 (MQ=255)                                                          cccAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATcttctt                                                                            >  4:353257/1‑135 (MQ=255)                                                                  tGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCTGATCTTCTTTAATACAtt                                                                   >  5:84117/1‑136 (MQ=255)                                                                  tGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttttt                                                                <  8:175342/139‑1 (MQ=255)                                                                  tGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAtt                                                                   >  5:291501/1‑136 (MQ=255)                                                                    aaTATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttttttt                                                              >  1:304872/1‑139 (MQ=255)                                                                    aaTATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACAttt                                                                  <  6:291501/135‑1 (MQ=255)                                                                     atatCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGAGCTTCTTTAATACAttttttt                                                              >  2:188579/1‑138 (MQ=255)                                                                       atCAAAAAAACGGATGAAGTCTGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATG‑AAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGCTGCAGCTCTTCTTTAATACAttttatt                                                              >  5:35042/1‑136 (MQ=255)                                                                       atCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGaa                                                                                                          <  6:98295/92‑1 (MQ=255)                                                                       atCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGaa                                                                                                          >  5:98295/1‑92 (MQ=255)                                                                        tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTg                                                             <  5:179008/136‑1 (MQ=255)                                                                        tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTg                                                             <  6:328830/136‑1 (MQ=255)                                                                        tCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgt                                                          <  1:314201/139‑1 (MQ=255)                                                                         cAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCgtc                                                         >  7:15577/1‑139 (MQ=255)                                                                         cAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTATTTAATACATTTTTTTGCgta                                                         >  1:357862/1‑138 (MQ=255)                                                                                  ggATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCt                                                <  1:96781/139‑1 (MQ=255)                                                                                                  aatcCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaa                                 <  1:85871/138‑1 (MQ=255)                                                                                                     ccGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaa                                 >  6:112196/1‑135 (MQ=255)                                                                                                      cGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCgg                                                                                          <  6:295102/77‑1 (MQ=255)                                                                                                      cGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCgg                                                                                          >  5:295102/1‑77 (MQ=255)                                                                                                      cGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCgg                                                                                          >  3:224098/1‑77 (MQ=255)                                                                                                      cGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCgg                                                                                          <  4:224098/77‑1 (MQ=255)                                                                                                      cGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaac                                >  3:386561/1‑135 (MQ=255)                                                                                                      cGAAACCGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTcaac                                >  6:424131/1‑135 (MQ=255)                                                                                                           ccGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACt                          >  4:133775/1‑136 (MQ=255)                                                                                                           ccGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACt                          <  5:36274/136‑1 (MQ=255)                                                                                                            cGCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCg                       >  7:354784/1‑138 (MQ=255)                                                                                                             gaaCTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTcc                        <  4:372572/134‑1 (MQ=255)                                                                                                             gCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                     <  2:141228/139‑1 (MQ=255)                                                                                                             gCACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCa                     <  8:305606/139‑1 (MQ=255)                                                                                                              cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                    >  1:187648/1‑139 (MQ=255)                                                                                                              cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                    >  7:57328/1‑139 (MQ=255)                                                                                                              cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                    >  7:249939/1‑139 (MQ=255)                                                                                                              cACTTTTTATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                    >  7:24609/1‑139 (MQ=255)                                                                                                                 tttttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                    >  3:108911/1‑136 (MQ=255)                                                                                                                 tttttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCAt                    >  4:92891/1‑136 (MQ=255)                                                                                                                 tttttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCACAt                    >  6:335111/1‑136 (MQ=255)                                                                                                                   tttATTTCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACA‑TTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCaa               <  2:102030/138‑1 (MQ=255)                                                                                                                       tttCGGGTGTCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAgg           <  8:302576/139‑1 (MQ=255)                                                                                                                           gggTGTCTGGATGC‑AAAAAACACAGCCGGATCGTCTGGAAAATCTCAACTATCCGGGTGCAGATCTTATTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACTAAACTCCGCATCGCAAAAGGctct       <  2:309013/139‑1 (MQ=255)                                                                                                                                tCTGCATGC‑AAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCtt  >  2:5496/1‑139 (MQ=255)                                                                                                                                   gcatgc‑aAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCtt  >  4:175986/1‑136 (MQ=255)                                                                                                                                    catgc‑aAAAAACACACCCGGTTCGTCTGGAAAATCTGATCAATCCGGGTGCAGATCTTCTTTACTACATTTTTTTGCGTCTGTCTTCCTAAGTCAAAAATTCAACAAAACTCCGCATCGCAATAGTCTCTTACtt  <  6:35042/135‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   GAAGCAAGCATACAATAGACATCTCTAGTAGAGGAAATCAACAAAGCAACGCTGTTACCCAGAAATGAATATCAAAAAAACGGATGAAGTCGGAATCAATCCGAAACCGCACTTTTTATTTCGGGTGTCTGCATGCAAAAAAACACACCCGGATCGTCTGGAAAATCTCAACAATCCGGGTGCAGATCTTCTTTAATACATTTTTTTGCGTCTGTCTTCCTCAGTCAAAAATTCAACAAAACTCCGCATCGCAAAAGGCTCTTCCTT  >  CP006881/3579649‑3579915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑