Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	924,071	(A)5→6	intergenic (‑51/‑228)	U712_04490 ← / → U712_04495	Vegetative catalase/Aliphatic sulfonates import ATP‑binding protein SsuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	924,066	1	.	A	100.0%	103.0 / NA	30	intergenic (‑46/‑233)	U712_04490/U712_04495	Vegetative catalase/Aliphatic sulfonates import ATP‑binding protein SsuB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (13/17);  total (13/17)

AGTTGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑AAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAAATAAT  >  CP006881/923934‑924196                                                                                                                                      |                                                                                                                                   aGTTGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaaaa                                                                                                                              <  7:114819/139‑2 (MQ=255)   ttGGTCCGCGAGAACCGGAAGTCATTTAGTTTTGATTATCTACAAACGGAGCGGCCCAGCTAGTTGTCAGATTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaaatgt                                                                                                                            <  8:77144/139‑4 (MQ=255)    tGGTCCTCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGAGCCGCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTTGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaaaagt                                                                                                                            <  2:308342/138‑4 (MQ=255)     ggTCCGCGAGAACCGGCAGTCATTGAGTTTAGATTATCTCCCACCGGAGCCCCCCAGCTAGTTGTCAGTTTATTTGAACTCAACTAAACACCTCTTGGAATTAATAAGAACATAATAACATCTCTAAGT‑aaaaaagtc                                                                                                                           >  1:308342/1‑134 (MQ=255)      gTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCaa                                                                                                                          >  7:126144/1‑139 (MQ=255)         cgcgAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaaaagtca                                                                                                                          <  3:207404/135‑6 (MQ=255)          gcgAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAat                                                                                                                         <  6:57439/136‑1 (MQ=255)                      aGTCATTGAGTTTTGCTTACCTCCAACCGGAGCGCCCCAGCTAGCTGGCAGTCTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAACTAAAAAAGGCAAAATTATTTTATAatta                                                                                                          <  8:126144/139‑1 (MQ=255)                      aGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGGTTTTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAAAATAATATCATCTCTAAGTAAAAAAGGCAATCTTATTTTATAatta                                                                                                          <  8:61289/139‑1 (MQ=255)                      aGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAatta                                                                                                          <  1:99675/139‑1 (MQ=255)                               gTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaaaagtca                                                                                                                          >  4:55668/1‑108 (MQ=255)                               gTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑aaaaaagtca                                                                                                                          <  3:55668/113‑6 (MQ=255)                                     aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGt                                                                                              >  3:27443/1‑136 (MQ=255)                                     aTTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGt                                                                                              >  5:179597/1‑136 (MQ=255)                                             cAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATctct                                                                                       <  4:27443/135‑1 (MQ=255)                                             cAACCGGAGCGCCCCAGCTAGGTGTCAGGTTATTTGAACTCATGTTATCCACTCTTGGAATTTATAAGCACAAAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTAtct                                                                                         <  4:145308/133‑1 (MQ=255)                                             cAACCGGAGAGCCCCAGCTAGTTGTCAGTTTAATTGAACTCATGTTATCACCTCTTTGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATctct                                                                                       <  5:346321/135‑1 (MQ=255)                                              aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTAt                                                                                     <  4:123321/136‑1 (MQ=255)                                              aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTAt                                                                                     <  6:264005/136‑1 (MQ=255)                                              aaCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTAt                                                                                     <  6:179597/136‑1 (MQ=255)                                               aCCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTAtt                                                                                    <  5:298342/136‑1 (MQ=255)                                                 cGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTaa                                                                                  <  6:220780/136‑1 (MQ=255)                                                       gcCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGata                                                                            >  6:292391/1‑136 (MQ=255)                                                            aGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTAt                                                                    <  8:365824/139‑1 (MQ=255)                                                                      cAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAAtt                                                             >  6:334011/1‑136 (MQ=255)                                                                                      aTGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCttgtt                                             <  3:32056/136‑1 (MQ=255)                                                                                           aTCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAg                                      <  7:402956/138‑1 (MQ=255)                                                                                            tCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACa                                       <  5:334011/136‑1 (MQ=255)                                                                                                  ctTGGAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTAt                              >  2:17818/1‑139 (MQ=255)                                                                                                     ggAATTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGa                            <  8:75047/138‑1 (MQ=255)                                                                                                        aTTTATAAGAACATAATATCATCTCTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGATATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGa                            >  3:66013/1‑135 (MQ=255)                                                                                                                             tctcTAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataa   >  7:269738/1‑139 (MQ=255)                                                                                                                              ctctAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGaaa      >  5:307212/1‑135 (MQ=255)                                                                                                                              ctctAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGaaa      >  5:215861/1‑135 (MQ=255)                                                                                                                              ctctAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataat  >  2:112334/1‑139 (MQ=255)                                                                                                                                ctAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataa   >  3:384576/1‑136 (MQ=255)                                                                                                                                 tAAGTAAAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAaataat  >  4:1655/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                   AGTTGGTCCGCGAGAACCGGCAGTCATTGAGTTTTGATTATCTCCAACCGGAGCGCCCCAGCTAGTTGTCAGTTTATTTGAACTCATGTTATCACCTCTTGGAATTTATAAGAACATAATATCATCTCTAAGT‑AAAAAGTCAATATTATTTTATAATTATTATAAAATAGTTATCTCTATTAATCGATATATAGTATCGAAATTGTTGAAAAACCTTGTTGTAACAGCTTTTTATGACACTTAATAATTTTTGGAGAAATAAT  >  CP006881/923934‑924196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑