| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,010 | C→T | E53E (GAG→GAA) | U712_19205 ← | Uncharacterized protein ywcI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,010 | 0 | C | T | 97.4% | 120.0 / NA | 38 | E53E (GAG→GAA) | U712_19205 | Uncharacterized protein ywcI |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/28); minor base G (1/0); total (10/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAA‑CGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACC > CP006881/3724877‑3725142 | tGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTACTTCCTCATTCACAGCCATCCGCCATTCAt < 7:95061/138‑1 (MQ=255) ccccATGAAAACATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTAAAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATCAACCGCCATCCGCAATTCATaa < 6:3924/134‑1 (MQ=255) cGCCATGTAAAGATGCGGGCCCGCTCTATCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCGATCCGCCATTCATaa < 6:192767/136‑1 (MQ=255) gCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTAGGGTTTTCTTCCGGATTCACAGCCATCCGCCATTCATAAAA‑c > 1:48684/1‑138 (MQ=255) tGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGa < 3:374642/136‑1 (MQ=255) aaaGATGCGGGCCCGCTCTCTCCTGCTGTGGCGATTCCTTGACGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAAcga < 2:39817/138‑3 (MQ=255) cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTATGCTTTTCTTGCTCATTGACCGCCCTCCGCCATTCATAAAA‑CGAAACGAGTcctgag > 8:229833/1‑135 (MQ=255) gacaggCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATATAGGGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGc < 7:403301/134‑1 (MQ=255) gCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTGTGCGTTTCTTCCTGATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCtgcg > 5:425690/1‑136 (MQ=255) ccgCTCTCTCCTGCTTTGGCGATTCCTTTATGGCTTGCAAACTTCTTATTTTTTAATGCGCGGGGGCTTTCGCTTTCTGCTTTTATACCTCATTCACAGCCATCGGCCATTCATAAAA‑CGAAACGAGTCCTGCGGc < 4:42257/134‑1 (MQ=255) ccgCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCGTTGTGCGGTTCTTCCTCATTGCCCGCCCGCCGCCCTTCATAAAA‑CGCAACCCGTCCAGCGGc > 3:46887/3‑136 (MQ=255) ccgCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGGTTTTCTTCCTCATTCAAAGCCATCCGCCATTCATTAAA‑CGAAACGAGTCCTGCGGc > 3:345412/3‑136 (MQ=255) tcCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCAcagc < 1:129988/137‑1 (MQ=255) cTGCTTTGGCGATTCCTTGAAGGATTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACagccgcc < 2:299038/139‑2 (MQ=255) cTTTGGCGATTCCTTGAAGGCTTGCAAACATCAACTTTAATAAAGCGCGGCGGCTTTCGCGTTAATGTTTTATTACTCAATCACACCCCTCCCCCAGTCATAAAA‑CGAAACGAGTCCTGCGGCCCGCAAcaccgcat > 8:403301/1‑135 (MQ=255) gCGATTCCTTGAAGGGTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCCTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt < 7:416567/138‑1 (MQ=255) gAAGGCTTGAAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGt < 3:256480/135‑1 (MQ=255) aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 7:21900/139‑1 (MQ=255) aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCCTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 8:43493/139‑1 (MQ=255) cgCTTGAAACCTTCTTCTTTATTAACGGGGGGCGGCTTTGGATTTGTGCTTTGCTTCCTCATTCCCCCCCACCCCCATTTCAT‑AAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg < 1:101369/138‑1 (MQ=255) gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 5:61767/136‑1 (MQ=255) gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGa < 2:69459/138‑1 (MQ=255) cAAACAGCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGcc < 5:359578/136‑1 (MQ=255) taACGCGCGGGGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCTTCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACTGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAg < 5:435830/136‑1 (MQ=255) cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑c < 2:48684/139‑1 (MQ=255) cTTTCGCTTTCTGCTTTTCTTCCTCAATCACAGCCATCCGCCATTCATAAAC‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCt < 5:257692/136‑1 (MQ=255) gcttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCCCCGCCGCCGTTGGTTTGGATGTTCGCGGAGGCGGATAGACCCCGGCCGCCCCCGGTTTGTGGT‑CCGGCggccc > 5:435630/3‑132 (MQ=255) ttcCTCATTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTATTTGGTTTTTTCGCTGAGGCGCATATAACCCGGCAGCCCCAGTTTTTCTGT‑GCAGCGGCACCCAGtt > 6:248871/1‑135 (MQ=255) aTTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCaaaaa < 1:114203/138‑1 (MQ=255) aTTCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCaaaa < 1:108639/137‑1 (MQ=255) ttCACAGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCaaaa < 6:425690/136‑1 (MQ=255) aGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGAcc < 5:270749/136‑1 (MQ=255) aGCCATCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGAcc < 3:104669/136‑1 (MQ=255) cactCCGCCATTCATAAAA‑CGAACCGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCca < 8:373078/136‑1 (MQ=255) cctCCCCCATTAATAAAACCAAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCca < 1:352659/137‑1 (MQ=255) tCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGa < 2:61959/129‑1 (MQ=255) tCCGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGa > 1:61959/1‑129 (MQ=255) ccGCCATTCATAAAA‑CGAAACGAGTCCTGCGGCCTGCACAGCCGCCGTTGTTTGGTTTGTACGCTGAGCCGCATAGACCGCGGCAGCCGCAGTTTTTCTGTGCCGGGGGC‑CCCAGGTGATCCAAAACACCAACCCCAcc > 7:149195/1‑139 (MQ=255) | TGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAA‑CGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGT‑CCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACC > CP006881/3724877‑3725142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |