BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,039	C→T	intergenic (–/‑939)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,039	0	C	T	98.8%	12949.2 / 69.8	3979	intergenic (–/‑939)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (28/21); new base T (2125/1799); total (2157/1822)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

Only 100 of 4029 total aligned reads displayed.

CTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTA  >  pSIJ663_pHT315_araE_xy/1903‑2173

cTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTAt                                                                                                                                      >  2:123559/1‑139 (MQ=255)

        cAACTTTATATCAATTAGTATATGTTCATTTAAAATCTTTACCAATTCAACTCTATTAGGTTCTTTAGGATTTTGCACGACATAGTCGGTGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATa                                                                                                                                 <  4:243607/136‑1 (MQ=255)

        cAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATa                                                                                                                                 <  3:369787/136‑1 (MQ=255)

        cAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATa                                                                                                                                 >  6:127374/1‑136 (MQ=255)

              tataGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATAc                                                                                                                                >  4:201732/1‑131 (MQ=255)

              tataGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAgg                                                                                                                            <  6:394811/135‑1 (MQ=255)

                taGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       <  1:100596/138‑1 (MQ=255)

                  gCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       >  5:187890/1‑136 (MQ=255)

                  gCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                       <  3:81727/136‑1 (MQ=255)

                      ttAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTtata                                                                                                                                     <  8:101844/118‑1 (MQ=255)

                          tataTGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGTGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTAc                                                                                                             <  8:179975/138‑1 (MQ=255)

                          tataTGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCttt                                                                                                               <  6:186327/136‑1 (MQ=255)

                            tatGTTCATTTAAAATCTTTACGAATTCAACGCTATTAGGTTCTTTATGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                          <  7:226489/139‑1 (MQ=255)

                            tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTAc                                                                                                             <  5:389228/136‑1 (MQ=255)

                             atGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATAACAGGATGTTGTTTCTTTACGTGc                                                                                                         >  2:76725/1‑139 (MQ=255)

                              tGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTtata                                                                                                                                     >  2:408329/1‑110 (MQ=255)

                                    tttAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  1:242121/1‑138 (MQ=255)

                                      tAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtat                                                                                                    >  6:220063/1‑135 (MQ=255)

                                       aaaaTCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                   >  4:105511/1‑135 (MQ=255)

                                             tttACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGa                                                                                          >  1:57531/1‑138 (MQ=255)

                                               tACCAATTCCAAGCTATTATGTTAGTTAGGATTTTGCAAGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGAGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCg                                                                                             <  3:33532/133‑1 (MQ=255)

                                               tACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGaaa                                                                                        >  7:193628/1‑138 (MQ=255)

                                                  cAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGaaa                                                                                        >  3:404929/1‑135 (MQ=255)

                                                     ttCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAtttt                                                                                  >  7:394523/1‑138 (MQ=255)

                                                     ttCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAtttt                                                                                  >  7:140/1‑138 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGATTATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  8:420932/138‑1 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAtttt                                                                                  >  3:308795/1‑135 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAtttt                                                                                  >  4:171215/1‑135 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAtttt                                                                                  >  4:88730/1‑135 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAtttt                                                                                  <  5:117986/135‑1 (MQ=255)

                                                        aaCGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  1:123089/138‑1 (MQ=255)

                                                               ttAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGt                                                                          >  2:159941/1‑136 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTtata                                                                                                                                     >  1:307848/1‑69 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAATTCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  1:187538/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               <  2:185543/123‑1 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                               >  7:166480/1‑123 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  8:33116/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  7:421325/1‑137 (MQ=255)

                                                                       tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  2:336073/1‑137 (MQ=255)

                                                                          acgATTTTGCACGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  2:425892/136‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  7:115273/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  7:332995/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             <  2:372421/138‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaa                                                               >  3:283024/1‑136 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGca                                                                <  4:134118/135‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGca                                                                <  5:221099/135‑1 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:87338/1‑134 (MQ=255)

                                                                          aGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                 >  5:248808/1‑134 (MQ=255)

                                                                           ggATTTTGCCCGACCTAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaa                                                               <  6:427816/135‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  7:368812/138‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  2:250004/138‑1 (MQ=255)

                                                                           ggATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaa                                                               <  5:288585/135‑1 (MQ=255)

                                                                            gATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             >  4:276756/1‑136 (MQ=255)

                                                                             aTTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  6:331132/136‑1 (MQ=255)

                                                                             aTTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtc                                                            <  5:419106/136‑1 (MQ=255)

                                                                             aTTTTGCCAGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaag                                                         <  2:285246/139‑1 (MQ=255)

                                                                                 tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTta                                                      >  8:251461/1‑138 (MQ=255)

                                                                                   cccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtata                                                    <  8:61265/138‑1 (MQ=255)

                                                                                   cccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTatat                                                   <  7:256779/139‑1 (MQ=255)

                                                                                    ccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTta                                                      >  5:148620/1‑135 (MQ=255)

                                                                                    ccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTta                                                      >  4:36178/1‑135 (MQ=255)

                                                                                    ccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATAGTTAGAAGTGCAAGTCAAGTtat                                                     >  6:64154/1‑136 (MQ=255)

                                                                                    ccGACATAGTCGGGGTGTTAAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtat                                                     <  4:50832/136‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtata                                                    <  5:352272/136‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtata                                                    <  6:87338/136‑1 (MQ=255)

                                                                                     cGACATAGTCGGGGTGTTCAACGATATATTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtata                                                    <  3:12698/136‑1 (MQ=255)

                                                                                       aCATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               <  1:90531/139‑1 (MQ=255)

                                                                                        cATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATc                                              <  1:157084/139‑1 (MQ=255)

                                                                                        cATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATc                                              <  1:336659/139‑1 (MQ=255)

                                                                                            gTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCt                                             <  3:54145/136‑1 (MQ=255)

                                                                                                gggTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATaa                                         >  6:306384/1‑136 (MQ=255)

                                                                                                gggTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGa                                      >  1:364111/1‑139 (MQ=255)

                                                                                                 ggTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAAt                                        <  5:319739/136‑1 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTTGAAGTGCAAGTCAAGTTATATGTATCTATAATGAtt                                    >  8:361552/1‑139 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGt                                                 >  8:383486/1‑126 (MQ=255)

                                                                                                  gtgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATc                                              >  8:159234/1‑129 (MQ=255)

                                                                                                     ttCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTTTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAtt                                    >  3:155365/1‑136 (MQ=255)

                                                                                                     ttCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGca                                                                <  3:21189/108‑1 (MQ=255)

                                                                                                     ttCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtat                                                     <  6:153512/119‑1 (MQ=255)

                                                                                                     ttCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgt                                 >  7:30403/1‑139 (MQ=255)

                                                                                                       cAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCTGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                             >  2:97443/1‑109 (MQ=255)

                                                                                                       cAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaa                                                               <  7:5833/107‑1 (MQ=255)

                                                                                                       cAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAttt                                   <  4:31366/135‑1 (MQ=255)

                                                                                                        aaagATATCTTTTATGTGCGATGAATATTTTTCATAAATACAAGGATGTTGTTTCTTTACGTTCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAATTGCAAGTCAAATTATATGTATCTATAATTATTtgt                                 >  3:250278/4‑136 (MQ=255)

                                                                                                          cGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgtg                                >  4:396949/1‑135 (MQ=255)

                                                                                                          cGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGGGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgtg                                >  6:275290/1‑135 (MQ=255)

                                                                                                             tatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAg                            <  4:337015/136‑1 (MQ=255)

                                                                                                             tatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGttt                         >  1:412704/1‑139 (MQ=255)

                                                                                                             tatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGttt                         >  1:385829/1‑139 (MQ=255)

                                                                                                               tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTAt                                               >  1:214168/1‑115 (MQ=255)

                                                                                                                  tttATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTg                       >  6:357352/1‑136 (MQ=255)

                                                                                                                    tATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCcac                   <  2:313047/138‑1 (MQ=255)

                                                                                                                      tgtgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCca                    <  6:246725/135‑1 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaag                                                         <  1:274130/94‑1 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  8:21473/1‑139 (MQ=255)

                                                                                                                          cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  2:213223/1‑139 (MQ=255)

                                                                                                                           gATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTg           >  8:12753/1‑139 (MQ=255)

                                                                                                                             tGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt            >  3:18522/1‑136 (MQ=255)

                                                                                                                             tGAATATTTTTTATAAATACCAGGATGTTCTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGt         >  8:295167/1‑139 (MQ=255)

                                                                                                                              gAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTg           >  5:61110/1‑136 (MQ=255)

CTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTA  >  pSIJ663_pHT315_araE_xy/1903‑2173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`