BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,049	C→T	intergenic (+318/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,049	0	C	T	99.9%	7647.9 / NA	2167	intergenic (+318/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (722/1442); minor base G (2/0); total (725/1442)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2201 total aligned reads displayed.

TTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGA  >  pSIJ663_pHT315_araE_xy/4914‑5184

  ttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                     <  7:363101/138‑1 (MQ=255)

   tCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGCGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                     >  7:415750/1‑137 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTa                                                                                                                                   <  5:346424/135‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTa                                                                                                                                   <  3:208514/135‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTa                                                                                                                                   <  5:77696/135‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTa                                                                                                                                   <  6:51920/135‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  8:180585/138‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  2:292594/138‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCGTTTTCCGAAGGTAACTGGCTTCAGCAGAGCCCAGCTACCAAATACTGTTCTTCTAgctg                                                                                                                               >  2:198328/1‑136 (MQ=255)

          gTAATGAGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGc                                                                                                                            <  1:236202/139‑1 (MQ=255)

          gTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTGTTTCCGAAGGTAACTGGCTTCAGCAGCGCGCAGATACCAAATACTGTTCTTCTAgtgt                                                                                                                               >  3:234764/1‑136 (MQ=255)

           tAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTa                                                                                                                              <  3:307725/136‑1 (MQ=255)

           tAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTa                                                                                                                              <  4:432040/136‑1 (MQ=255)

           tAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                           <  7:195443/139‑1 (MQ=255)

           tAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTATCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTa                                                                                                                              <  5:265043/136‑1 (MQ=255)

                  ctgctTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGt                                                                                                                      <  8:66116/137‑1 (MQ=255)

                   tgctTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTTTTTGCCGGATCAAGAGCTACCAACTCTGTTTCCGAAGGTAACTGGCTTCAGCAGCGCGCAGATACCAAATACTGTTCTTCTAGTTTAGCCCTAGTTAg                                                                                                                   >  8:403302/1‑139 (MQ=255)

                     ctTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCGTTTTCCGAAGGTAACTGGCTTCACCAGAGCGCAGATACCAATTACTGTTCTTGTAGTGTAGCCGTAGttt                                                                                                                    >  6:292702/1‑135 (MQ=255)

                       tGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAgg                                                                                                                  >  6:376959/1‑136 (MQ=255)

                         caaacaaaAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  2:334522/139‑1 (MQ=255)

                         caaacaaaAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  8:328901/139‑1 (MQ=255)

                         caaacaaaAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  7:327864/138‑1 (MQ=255)

                         caaacaaaAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAAAACTCTTCTTCTTGAGTAGCCGTAGTTAGGcc                                                                                                                >  4:165388/1‑136 (MQ=255)

                          aaacaaaAAAAACACCCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  2:237550/139‑1 (MQ=255)

                                 aaaaCCA‑CCTCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                     <  7:226348/139‑1 (MQ=255)

                                 aaaaCCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                     <  8:379933/139‑1 (MQ=255)

                                   cacca‑ccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCaa                                                                                                      <  5:286972/135‑1 (MQ=255)

                                   aaCCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCaa                                                                                                      <  4:211619/136‑1 (MQ=255)

                                   aaCCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  2:8942/139‑1 (MQ=255)

                                   aaCCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  7:145386/139‑1 (MQ=255)

                                   aaCCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  7:56064/139‑1 (MQ=255)

                                   aaCCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  1:329714/139‑1 (MQ=255)

                                   aaCCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  1:159353/139‑1 (MQ=255)

                                     cca‑ccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGa                                                                                                    <  5:238353/136‑1 (MQ=255)

                                         ccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCCCCACTTCAAGAACTCTg                                                                                              >  8:26114/1‑139 (MQ=255)

                                         ccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTACCGAAGGTAACTGGCTTCAGCACAGCGCAGATACCAAATACAGTTCTTCTAGTGTAGCCGTATTTAGGCCCCACCTTCAAGAActcag                                                                                              >  2:396029/1‑137 (MQ=255)

                                            cTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc                                                                                                        <  6:257093/126‑1 (MQ=255)

                                            cTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCaa                                                                                                      <  3:78168/128‑1 (MQ=255)

                                            cTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTg                                                                                              >  4:70882/1‑136 (MQ=255)

                                            cTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCGTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTGGCCGTAGTTAGGCCGCCACTTCAATCACTCTg                                                                                              >  6:31560/1‑136 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTCGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                           <  5:173977/136‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                           <  6:80359/136‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                           <  5:105988/136‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                           <  3:115392/136‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                           <  3:175460/136‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                           <  3:414155/136‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  1:132494/139‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  1:213114/139‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  1:393477/139‑1 (MQ=255)

                                               ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  1:98543/139‑1 (MQ=255)

                                                    ggtggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGc                                                                                          <  2:62447/132‑1 (MQ=255)

                                                      tggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGcc                                                                                    <  4:108585/136‑1 (MQ=255)

                                                         tttgtttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAc                                                                                 <  4:402723/136‑1 (MQ=255)

                                                           tgtttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc                                                                            >  8:152966/1‑139 (MQ=255)

                                                             tttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                     >  2:363745/1‑80 (MQ=255)

                                                               tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc                                                                            <  4:250586/135‑1 (MQ=255)

                                                               tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc                                                                            <  6:335305/135‑1 (MQ=255)

                                                               tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                         <  8:227729/138‑1 (MQ=255)

                                                                gCCGGATCAAGATCTAGCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCTCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCAACCTCTTCCAGTACTCTGTAGCACCGCCTACATACCTCGc                                                                        <  7:80337/138‑1 (MQ=255)

                                                                 ccGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctc                                                                      <  1:74650/139‑1 (MQ=255)

                                                                         agagCTGCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  7:298267/102‑1 (MQ=255)

                                                                         agagCTGCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   >  8:298267/1‑102 (MQ=255)

                                                                         agagCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATc                                                              >  8:118534/1‑139 (MQ=255)

                                                                            gCTACCAAATCTTTTTCCGAAGGTAACTGGGTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTTGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAAtg                                                              <  6:118880/136‑2 (MQ=255)

                                                                             cTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATcc                                                             <  3:381694/136‑1 (MQ=255)

                                                                               aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                     <  7:166684/62‑1 (MQ=255)

                                                                               aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  7:390756/107‑1 (MQ=255)

                                                                               aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAc                                                                             >  1:368542/1‑118 (MQ=255)

                                                                               aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGct                                                                       >  2:133502/1‑124 (MQ=255)

                                                                                  aaCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  5:367416/82‑1 (MQ=255)

                                                                                  aaCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCCCCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTa                                                        >  3:149950/1‑136 (MQ=255)

                                                                                  aaCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctc                                                                      >  6:221164/1‑122 (MQ=255)

                                                                                  aaCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTa                                                        >  6:151765/1‑136 (MQ=255)

                                                                                  aaCTCTATTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCa                                                     <  7:107362/139‑1 (MQ=255)

                                                                                   aCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc                                                                            >  4:273375/1‑115 (MQ=255)

                                                                                     tctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  8:431201/90‑1 (MQ=255)

                                                                                     tctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTg                                                  >  1:420971/1‑139 (MQ=255)

                                                                                        ttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                           >  5:318183/1‑95 (MQ=255)

                                                                                             cGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgctgc                                             >  5:209505/1‑136 (MQ=255)

                                                                                                 ggTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                           >  5:405622/1‑86 (MQ=255)

                                                                                                    aaCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGACAGt                                      >  3:353679/1‑136 (MQ=255)

                                                                                                         gCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGc                                   <  3:51467/134‑1 (MQ=255)

                                                                                                          cTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAt                                <  4:190818/136‑1 (MQ=255)

                                                                                                           ttCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGt                            >  1:291166/1‑139 (MQ=255)

                                                                                                            tCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        >  2:263465/1‑78 (MQ=255)

                                                                                                             cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAt                                >  2:156761/1‑133 (MQ=255)

                                                                                                              agcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGt                            >  4:35394/1‑136 (MQ=255)

                                                                                                               gcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGc                                   <  5:48728/128‑1 (MQ=255)

                                                                                                               gcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTc                           <  5:135791/136‑1 (MQ=255)

                                                                                                                cagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAg                                                    >  6:410268/1‑110 (MQ=255)

                                                                                                                   agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCt                     >  2:45551/1‑138 (MQ=255)

                                                                                                                   agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAG‑TAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                       >  8:350533/1‑71 (MQ=255)

                                                                                                                    gcgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTc                      <  4:322526/136‑1 (MQ=255)

                                                                                                                     cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAc                  >  1:100480/1‑139 (MQ=255)

                                                                                                                      gcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc                                                                                                        <  6:184308/52‑1 (MQ=255)

                                                                                                                         gATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCggg              <  1:263956/139‑1 (MQ=255)

                                                                                                                          aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCCCCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAACTCGTGTCTGAGCGGGt             >  1:18604/1‑139 (MQ=255)

                                                                                                                           tACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCgg               <  4:13886/136‑1 (MQ=255)

                                                                                                                               aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGAc        >  7:256121/1‑139 (MQ=255)

                                                                                                                                aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCATTGGCTGCTGCCAGTGGAGATAAGTCGGTTCTTTCCGGGTTgg          >  3:271906/1‑136 (MQ=255)

TTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA‑CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGA  >  pSIJ663_pHT315_araE_xy/4914‑5184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`