BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,205	G→A	intergenic (+474/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,205	0	G	A	96.7%	8587.7 / ‑2.2	2890	intergenic (+474/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base G (24/17); major base A (1295/1449); minor base C (83/12); total (1405/1485)
Fisher's exact test for biased strand distribution p-value = 8.29e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2896 total aligned reads displayed.

TAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATC  >  pSIJ663_pHT315_araE_xy/5069‑5341

    ccaccaCTTCAAGAACTCTGTAGCACCGCATACATACCTCGATCTGCTAATCCTGTTACCAGTGGCTGCTGAGAGTGGCGATTAGTCGTGTCTTACCGGGATGGACTCAAGACGATAGTTACCGGATAAGGcacat                                                                                                                                       <  4:307221/136‑2 (MQ=255)

     caccacTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGGCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGt                                                                                                                                   <  7:407491/139‑1 (MQ=255)

      accacTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCg                                                                                                                                     >  5:301513/1‑136 (MQ=255)

       ccacTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGGCGAGTCTTACCGGGTTGGACTGAGGAAGATATATACCGGATAAGGCACACCGGTCg                                                                                                                                 >  2:361975/1‑139 (MQ=255)

          cTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCg                                                                                                                                 <  5:328689/136‑1 (MQ=255)

          cTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGGCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGc                                                                                                                              <  7:118534/139‑1 (MQ=255)

          cTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCCGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTCCCGGGTTGGACTCAAGACCTTTGTTACCGGAAAAGCCACCGCGGTCg                                                                                                                                 >  5:511/1‑136 (MQ=255)

           ttCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAACCGGGTGGACGAAAGACGATATTTAAAGGGAAAGGCACAGCgctggg                                                                                                                                >  3:250500/1‑131 (MQ=255)

             cAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCTGTGGCGATAAGTAGTGTGTTTCCGGGTTGGACTCAAGACGATAGTTTCCGGATTAGGCACCGCGGGCGGGCTg                                                                                                                            >  2:75317/1‑138 (MQ=255)

                     ctGTAGCACCGCCTACATACCTCGCTCTGCTAATGCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                                                   <  1:210661/139‑1 (MQ=255)

                        tAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTGATTCCCGGTTTGACTAAAGACGATAGTTAACCGATAAGGGCCCCCCGTCCGGCTGAAAGGGGGGt                                                                                                                  >  8:292193/1‑137 (MQ=255)

                        tAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAg                                                                                                                                       <  6:354455/116‑1 (MQ=255)

                        tAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGGCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggggg                                                                                                                   <  1:24767/136‑1 (MQ=255)

                         aGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGt                                                                                                                  >  4:281338/1‑136 (MQ=255)

                          gCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGtt                                                                                                                 <  3:28254/136‑1 (MQ=255)

                               gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGc                                                                                                            <  6:102361/136‑1 (MQ=255)

                               gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGc                                                                                                            <  4:379208/136‑1 (MQ=255)

                               gCCTAAAAAACTCGCTCTGCTAATCCCGTTTCCAGTGGCTGCTGCCAGGGGCGATAAGGCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGc                                                                                                            <  6:511/136‑1 (MQ=255)

                                ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGc                                                                                                                              <  8:120519/117‑1 (MQ=255)

                                ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                                                        >  8:2055/1‑139 (MQ=255)

                                ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                                                        >  8:238732/1‑139 (MQ=255)

                                ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacac                                                                                                        >  1:10026/1‑139 (MQ=255)

                                 cTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcac                                                                                                          >  5:411144/1‑136 (MQ=255)

                                 cTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcac                                                                                                          >  6:194950/1‑136 (MQ=255)

                                   aCATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTGTTACCGGGTTGGACTCAAGCCCCTAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGGTCGTGcaca                                                                                                         >  3:31151/1‑135 (MQ=255)

                                   aCATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGGTAGTTTACGGCTAAGGCACACCGGTGGGGCTGGACGGGGGGTTGGTggccac                                                                                                        >  3:12157/1‑131 (MQ=255)

                                   aCATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGCTAGTTACCGGATAAGGCACCGCGGTCGGGCTGAACGGGGGGTTCGTGcccac                                                                                                        >  4:191890/1‑136 (MQ=255)

                                    cctACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGc                                                                                                     <  8:324382/136‑1 (MQ=255)

                                     aTACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcaca                                                                                                         >  1:50416/1‑133 (MQ=255)

                                         cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATTAGGCACAGCGGTCGGGCTGCACGGGGGGTTCGTGCACACAGCCCAGcg                                                                                               >  2:48070/1‑138 (MQ=255)

                                          tCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCtt                                                                                              >  7:315579/1‑139 (MQ=255)

                                           cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                             >  7:227015/1‑139 (MQ=255)

                                           cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCGTACCGGGTTGGCCTCAAGACGATATTTACCGGATAAGGCACAGCGGTCGGGCTTAACGGGGGGTTCGTGCCCACCGCCCAGc                                                                                                >  3:98321/1‑136 (MQ=255)

                                           cGCTCTGCTAATCCTGTTACCAGTGGCTGATGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGACAGAACACGATAGTTACCGGATAATGAACAGCGTGAGGGCTGAACCGGGGCTTGCTGCACACAGCCCAGCTTg                                                                                             >  1:127409/1‑139 (MQ=255)

                                              tctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGCCTCAAGCCGATAGTTACCGGATAAGGCACAGCGGGCGGGCGGAACGGGGGGTTCGTGCCAACACCCCCGCTTg                                                                                             >  5:36111/1‑136 (MQ=255)

                                              tctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCCGCGGTCGGGCTGAACGGGGGGTTCGGGCACACAGCCCAGCTTg                                                                                             >  4:117564/1‑136 (MQ=255)

                                              tctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                             >  6:378707/1‑136 (MQ=255)

                                              tctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGGCTCAAGCCGATAGTTTCCGGTTTAGGCACAGCGGTCGGGGTGAACCGGGGGTTCGTGGACCCAGCCCCGCGTg                                                                                             >  4:16576/1‑136 (MQ=255)

                                              tctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGGCTTACCGGGTTGGGCGCAAGACGATAGTTTCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTGGGTGCACACAGCCCCGcatctgt                                                                                          >  2:178861/1‑133 (MQ=255)

                                               ctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTgg                                                                                            >  3:374875/1‑136 (MQ=255)

                                                  cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTTGACGCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGGTGAACGGGGGGGTCGTGCACACAGCCCCGCGGGGAGCgga                                                                                      >  2:112602/1‑137 (MQ=255)

                                                  cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGaa                                                                                      <  8:45455/139‑1 (MQ=255)

                                                  cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                       <  8:267618/138‑1 (MQ=255)

                                                      tCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAAc                                                                                     <  4:144353/136‑1 (MQ=255)

                                                        cTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGa                                                                                   >  4:212008/1‑136 (MQ=255)

                                                          gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAg                                                                                                                                       >  8:168447/1‑82 (MQ=255)

                                                          gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGc                                                                                                                              <  1:281368/91‑1 (MQ=255)

                                                          gTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTa                                                                               >  7:250905/1‑138 (MQ=255)

                                                           ttACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCt                                                                                <  5:22368/136‑1 (MQ=255)

                                                           ttACCAGTGGCTGCTGCCAGGGGCGATAAGTCGTGGCTTACCGGGTTGGCCTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTaca                                                                             <  2:401041/139‑1 (MQ=255)

                                                           gtACCAGTGGTTGCTGCCAGTGGAGTTAAGTGGTGTCTTCCAGGGTGGGAGGCAAGACGATAGTTACCGGATAAGGCACAGCGGGCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTaca                                                                             <  2:212725/138‑1 (MQ=255)

                                                            tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGt                                                                                                              <  2:285076/105‑1 (MQ=255)

                                                            tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTacac                                                                            >  7:59683/1‑139 (MQ=255)

                                                            tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTGCGTGCACCCAGCCCAGCTGGGGGCGAACGCCCTaccc                                                                            >  1:39884/1‑137 (MQ=255)

                                                             aCCAGTGGCTGCTGCCAGTGGCGATAGGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAAAAGGCACAGCGGTCGGGCGGAACGGGGGGGTCGTGCACACCGCCCCGCTGGGAGCGCAcgaccgac                                                                              >  4:40333/1‑136 (MQ=255)

                                                             aCCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGCCGCAAGACGCTAGTTACCGGTTAAGGCACAGCGGTCGGGCTGAACGGGGGGGTCGTGCACACAGCCCAGCTTGGGGCGCACGCCCTac                                                                              >  5:142937/1‑136 (MQ=255)

                                                             aCCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACCGCGGTCGGGCTGAACGGGGGGGTCGTGCACACCGCCCAGCTTGGAGCGCACGACCTac                                                                              >  6:82654/1‑136 (MQ=255)

                                                             aCCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAg                                                                                                                                       >  4:75353/1‑79 (MQ=255)

                                                               cAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTacac                                                                            >  6:161910/1‑136 (MQ=255)

                                                               cAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAACGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGAGGTCGGGCTGAACTGGGGGTTCTTGCACACATCCCAGCTTGGAGCGAACGAcagacaa                                                                            >  5:387806/1‑130 (MQ=255)

                                                                cgccgctgctGCCAGCGGCGACAACTCGAGTCTTACCGGGCCGGACGCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACAcc                                                                           <  6:223015/132‑1 (MQ=255)

                                                                aGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa                                                                        <  7:279584/139‑1 (MQ=255)

                                                                aGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa                                                                        <  1:30584/139‑1 (MQ=255)

                                                                  tGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGa                                                                         <  5:194950/136‑1 (MQ=255)

                                                                   ggCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACAcc                                                                           >  3:102563/1‑133 (MQ=255)

                                                                    gctgctGCCAGTGGCGATAAGTCGTGTCTTTCCGGGTTGGGCTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCACCTTGGCGCGAACGCCCGACACCCaaa                                                                       >  4:249393/1‑135 (MQ=255)

                                                                    gctgctGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAAc                                                                                     >  5:81747/1‑122 (MQ=255)

                                                                     ctgctgCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACGCAAGACGATATTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGGACACAGCCCCGCGTGGAGCGAACGACCTACACCGAACt                                                                      >  1:156372/1‑136 (MQ=255)

                                                                      tgctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTGCGACTCAAGACGATAGTTACCGGATAAGGCTCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGTACGACCTACACCGAACTgaga                                                                  <  7:370005/139‑1 (MQ=255)

                                                                        ctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTg                                                                     <  4:432314/134‑1 (MQ=255)

                                                                        ctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTg                                                                     <  6:137254/134‑1 (MQ=255)

                                                                            cAGTGGCGATAAGTCGTGGCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTa                                                            <  1:185000/139‑1 (MQ=255)

                                                                                 gCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGGCGGGCTGAACGGGGGGGTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCg                                                       <  1:335587/139‑1 (MQ=255)

                                                                                   gATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg                                                     <  2:137966/139‑1 (MQ=255)

                                                                                   gATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACAGGGGGTGCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTg                                                     <  1:364048/139‑1 (MQ=255)

                                                                                                      ggTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACCGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAgcgc                                     >  5:256933/1‑136 (MQ=255)

                                                                                                        ttGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGc                                <  8:77156/139‑1 (MQ=255)

                                                                                                        gtGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCa                                   <  3:191040/135‑1 (MQ=255)

                                                                                                          ggACTCAAGCCGATTGTTACCGCATAAGGCACCGCGGTCGGGCTGACCGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGACATACCTACAGCGTGAGCTATGAGAAAGCGCCACg                                 <  4:336837/136‑1 (MQ=255)

                                                                                                           gACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTa                                                                               >  6:415542/1‑89 (MQ=255)

                                                                                                           gACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACAcc                                                                           >  5:109692/1‑93 (MQ=255)

                                                                                                             cTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCtt                              >  6:314008/1‑136 (MQ=255)

                                                                                                                     gATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAgg                      >  1:76338/1‑136 (MQ=255)

                                                                                                                       tAGTTACCGTATAAGGCACAGCGGGCGGGCTGAAGGGGGGGTTCGTCCACACAGCCCAGCTTGGAGCGAACTACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGga                    <  6:263444/136‑1 (MQ=255)

                                                                                                                       tAGTTACCGGATACGCCACAGCGGTCGGGCGGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCAACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGaa                 <  7:92636/139‑1 (MQ=255)

                                                                                                                       tAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTTAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGaa                 <  2:253973/139‑1 (MQ=255)

                                                                                                                       tAGTAACCGGACACGGCACAGCGGGCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTGGGAGCGAACGACCTACACCGAACTGAGATACCTACCGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGga                    <  3:40333/136‑1 (MQ=255)

                                                                                                                        aGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGCGCTATGAGAAAGCGCCACGCTTCCCGAAGGga                    <  3:341599/135‑1 (MQ=255)

                                                                                                                            aCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCtt                                                                                              >  7:184085/1‑57 (MQ=255)

                                                                                                                             ccGGATACGGCACAGCGGGGGGGCTGAACGGGGGGTTCTGGCACACACCCCAGCTTGGAGCGAACTACCTACACCGAACTGAGCTACCTACAGCGTGAGCTATGCGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCg            <  8:356561/138‑1 (MQ=255)

                                                                                                                             ccGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAgg              >  3:144106/1‑136 (MQ=255)

                                                                                                                             ccGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTCCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAg               <  3:43179/135‑1 (MQ=255)

                                                                                                                              cGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTgaga                                                                  <  7:162954/83‑1 (MQ=255)

                                                                                                                              cGGATAAGGCACAGCGGGCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGa          <  7:340985/139‑1 (MQ=255)

                                                                                                                               ggATAAGGCACAGCGGTCGTCCTGATCGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTCCACCCAACTGAGATACCAACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGCAGAAAGGCg            >  3:166586/1‑136 (MQ=255)

                                                                                                                               ggATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAATCGCCACGCTTCCCGAAGGGAGAAAGGCg            >  3:68554/1‑136 (MQ=255)

                                                                                                                               ggATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCg            >  3:213812/1‑136 (MQ=255)

                                                                                                                                    aGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATAc                                                               >  5:163081/1‑80 (MQ=255)

                                                                                                                                     ggCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                       >  2:321563/1‑55 (MQ=255)

                                                                                                                                     ggCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGt                                                      <  8:388973/88‑1 (MQ=255)

TAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATC  >  pSIJ663_pHT315_araE_xy/5069‑5341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`