Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,474	A→G	R96R (CGA→CGG)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,474	0	A	G	97.5%	288.0 / ‑2.3	82	R96R (CGA→CGG)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (29/49);  total (31/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

TCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCC‑GAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTT  >  CP006881/3723341‑3723605                                                                                                                                       |                                                                                                                                    tcttTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCGATTATGATGATTTCTTTCGGGGGCGGGGATTTATTTTCCGGCCACACCATTTTTTTGACCAATACCGCGCTCC‑Ggaaaa                                                                                                                                 >  2:299541/1‑138 (MQ=255)        ggCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACCCCGTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAAt                                                                                                                             >  4:100972/1‑135 (MQ=255)             aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTgg                                                                                                                      <  8:392421/137‑1 (MQ=255)             aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    >  8:115089/1‑139 (MQ=255)             aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    >  7:356755/1‑139 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGGTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTAAACCGCGCTCC‑GGAAAAAAATCAGCTGGCgcg                                                                                                                  <  8:96928/138‑3 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAACTCAGCTGGc                                                                                                                     <  6:305937/135‑1 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGc                                                                                                                     <  5:121395/135‑1 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGc                                                                                                                     <  3:70578/135‑1 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCgcg                                                                                                                  <  7:175593/138‑3 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCgcg                                                                                                                  <  1:332764/138‑3 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCgcg                                                                                                                  <  1:367152/138‑3 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATGCTGATGATTGCATACGGAGTCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    <  3:39808/136‑1 (MQ=255)                ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGACGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGc                                                                                                                     <  3:325101/135‑1 (MQ=255)                ctcCGTTTGCCTTTCCCGCAGCCGCGGTCACTATCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTAATTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGc                                                                                                                     <  6:113012/135‑1 (MQ=255)                ctcCGTTTACCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGATAAAAATCAGCTTGCg                                                                                                                    >  4:267020/1‑136 (MQ=255)                   cttttGCCTTTCGGTCAGCCGGTGTCACTTTTGGTGCCGATATTCTTATTATTGCTTACGGAGGCGGAGATTTATTTAGCGGAAACACATTTTATTTCACCTTTACGGCGTTCG‑GTAAAAATTTCTGCTGGCg                                                                                                                    <  4:241089/131‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGTGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGGGCTCC‑GGAAAAAAATCAGCTGGCgcga                                                                                                                 <  2:323921/136‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCgcga                                                                                                                 <  2:194228/136‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCgcga                                                                                                                 <  2:295792/136‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCgcga                                                                                                                 <  2:79501/136‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    <  4:403109/133‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    <  4:361878/133‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    <  3:262657/133‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    <  3:252670/133‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    <  3:180769/133‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGaca                                                                                                               <  7:192845/138‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGaca                                                                                                               <  8:356755/138‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCGTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGaca                                                                                                               <  1:93245/138‑1 (MQ=255)                   cGTTTGCCTTTCCGGCAGCCGCTGTCACTTGCGGGGCCGCTATTCTGATGATTCATAACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCgcga                                                                                                                 <  1:313461/136‑1 (MQ=255)                   cGTTTGCCTATCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACATTTTATTTAAACTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    <  6:47573/133‑1 (MQ=255)                    gTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGac                                                                                                                <  3:254252/136‑1 (MQ=255)                    gTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACAcc                                                                                                             <  1:299541/139‑1 (MQ=255)                    gTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACAcc                                                                                                             <  1:247388/139‑1 (MQ=255)                      ttGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑G‑GAAAAAATCAGCTGGCGCGacaca                                                                                                             >  5:76476/1‑135 (MQ=255)                          ctttCCGGCAGCCGCTGTCACTTTCGGGGACGCTATTCTGATGATTGCTTTCGGAGGCGGAGATTTATTTAGCGTCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCta                                                                                                          <  4:315220/135‑2 (MQ=255)                               cGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATAct                                                                                                      <  6:92651/135‑2 (MQ=255)                                ggCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    >  3:283031/1‑120 (MQ=255)                                ggCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCg                                                                                                                    <  4:283031/120‑1 (MQ=255)                                ggCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATAct                                                                                                      <  5:408258/134‑2 (MQ=255)                                ggCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATAct                                                                                                      >  6:408258/1‑133 (MQ=255)                                                     ggCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAAtcagc                                                                                                                         <  2:230515/94‑5 (MQ=255)                                                     ggCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAAtcagc                                                                                                                         >  1:230515/1‑90 (MQ=255)                                                          cTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAAttt                                                                          <  5:204262/136‑1 (MQ=255)                                                          cTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                                                       <  1:372619/139‑1 (MQ=255)                                                          cTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                                                       <  7:335914/139‑1 (MQ=255)                                                          cTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                                                       <  1:56013/139‑1 (MQ=255)                                                                       tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑Ggaa                                                                                                                                   <  7:180338/66‑1 (MQ=255)                                                                       tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑Ggaa                                                                                                                                   >  8:180338/1‑66 (MQ=255)                                                                       tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                                                           >  7:380000/1‑138 (MQ=255)                                                                       tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                                                           <  8:380000/138‑1 (MQ=255)                                                                          ttACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAttatg                                                          >  4:62971/1‑133 (MQ=255)                                                                          ttACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGAGATCC‑GGATAAAAATCAGCTGGCGCGACAACCTATAATTGTGGATGTCAAGCTATGCCTGCAATTTAATCGGCGCCATTCtg                                                          >  5:156830/1‑135 (MQ=255)                                                                            aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                      <  7:115089/138‑1 (MQ=255)                                                                            aCGGAGGCGGAGATTTATTTACCGGCAACACATTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGc                                                     >  2:68229/1‑139 (MQ=255)                                                                                     gagaTTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCt                                               >  6:394267/1‑136 (MQ=255)                                                                                      agaTTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTGAGTTTGCCagactg                                              >  5:273358/1‑131 (MQ=255)                                                                                      agaTTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCt                                               >  6:367356/1‑135 (MQ=255)                                                                                      agaTTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCt                                               >  3:116697/1‑135 (MQ=255)                                                                                                accggCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGAcgg                                 >  8:307840/4‑137 (MQ=255)                                                                                                 ccggCAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGAc                                   <  5:151535/134‑1 (MQ=255)                                                                                                 ccggCAACACCTTGTAATTCACCTATCCCGCGCTCC‑GGAAAAAAAGCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACggg                                <  2:271479/137‑1 (MQ=255)                                                                                                     cAACACCTTTTATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGAGCAGCGCGACggga                               >  5:29638/1‑135 (MQ=255)                                                                                                           cttttATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACttttt                         <  4:116697/135‑1 (MQ=255)                                                                                                           cttttATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                      <  7:98998/138‑1 (MQ=255)                                                                                                             tttATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGCCACCCTATACTTGTGGAAGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                      >  1:107703/1‑137 (MQ=255)                                                                                                              ttATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                    <  1:68229/138‑1 (MQ=255)                                                                                                              ttATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTga                       <  5:367356/135‑1 (MQ=255)                                                                                                               tATTTCACCTATACCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagg                     >  5:229496/1‑136 (MQ=255)                                                                                                                     acctataCCGCGCTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTGGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTg            >  2:417105/4‑139 (MQ=255)                                                                                                                         atcccGCGCACC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCCTTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGt           <  6:379564/133‑1 (MQ=255)                                                                                                                             cgcgCTCCGGAAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCtgt                                                         <  3:254440/87‑3 (MQ=255)                                                                                                                             cgcgCTCCGGAAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCtgt                                                         >  4:254440/1‑85 (MQ=255)                                                                                                                              gcgcTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGAGCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTcgt   >  1:397877/1‑137 (MQ=255)                                                                                                                               cgcTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCgg                                                                    >  8:430158/1‑73 (MQ=255)                                                                                                                               cgcTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCgg                                                                    <  7:430158/73‑1 (MQ=255)                                                                                                                               cgcTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt  >  8:253873/1‑139 (MQ=255)                                                                                                                               cgcTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt  >  1:155353/1‑139 (MQ=255)                                                                                                                               cgcTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt  >  7:16888/1‑139 (MQ=255)                                                                                                                                 cTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                      >  1:418618/1‑85 (MQ=255)                                                                                                                                 cTCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                      <  2:418618/85‑1 (MQ=255)                                                                                                                                  tCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGt           <  5:104054/127‑1 (MQ=255)                                                                                                                                  tCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGt           >  6:104054/1‑127 (MQ=255)                                                                                                                                  tCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt  >  5:68400/1‑136 (MQ=255)                                                                                                                                  tCC‑GGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt  >  5:9563/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                                    TCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCC‑GAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTT  >  CP006881/3723341‑3723605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑