Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,307,456	T→C	Y305C (TAT→TGT)	U712_11670 ←	Uncharacterized protein yqjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,307,456	0	T	C	100.0%	184.4 / NA	50	Y305C (TAT→TGT)	U712_11670	Uncharacterized protein yqjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/33);  total (17/33)

CATGAAAATCGAACATAAGAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAATATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTATGCTG  >  CP006881/2307320‑2307585                                                                                                                                         |                                                                                                                                  catGAAAATCGAACATAAGAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAacat                                                                                                                                 >  1:241596/1‑139 (MQ=255)               gtAAGAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTc                                                                                                                      <  3:90326/135‑1 (MQ=255)                 aaGAAAACACGCATAAGCTCCTCCTTTTTCATTAGGTTGACGAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCTCTAAACATTGTTCCATTTCGc                                                                                                                    <  6:333482/136‑1 (MQ=255)                 aaGAAAAAACGCATAAGCTCCTCCTTTTTGATTATGTTTACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGc                                                                                                                    <  5:54052/136‑1 (MQ=255)                 aaGAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGc                                                                                                                    <  5:156954/136‑1 (MQ=255)                  aGAAACAACGCATCAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGc                                                                                                                    <  3:289122/135‑1 (MQ=255)                  aGAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCga                                                                                                                 <  2:241596/138‑1 (MQ=255)                   gAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGcc                                                                                                                   >  3:300058/1‑135 (MQ=255)                   gAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCgaga                                                                                                               <  8:211918/139‑1 (MQ=255)                   gAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCg                                                                                                                  <  5:363538/136‑1 (MQ=255)                   gAAAAAACGCAAAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAAATTTTAATCACTAAACAATGTTCCATTTCGcc                                                                                                                   >  5:91497/1‑135 (MQ=255)                     aaaaaCCCATATGCTCCTCCTTTTTCATTAGGTTGACCAATGAATGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTAAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGGCgag                                                                                                                <  6:260932/136‑1 (MQ=255)                      aaaaCGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCgaga                                                                                                               <  5:10397/136‑1 (MQ=255)                       aaaCGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGAt                                                                                                           >  1:20596/1‑139 (MQ=255)                           gCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATg                                                                                                          <  6:252014/136‑1 (MQ=255)                             aTAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGATCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCgag                                                                                                                >  8:51948/1‑128 (MQ=255)                                aGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAagagag                                                                                                  <  8:346801/139‑1 (MQ=255)                                aGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAaga                                                                                                     <  6:147081/136‑1 (MQ=255)                                    cctcctTTTTCATTAGGTTCACCAATGAAGGATGAGCTCATGCGAAACATCCGAGTGATTTTGGCGGTTGTATTCCTGTAAAATAACTTGTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCTGc                                                                                              >  2:411996/1‑139 (MQ=255)                                     ctcctTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCg                                                                                                <  5:185898/136‑1 (MQ=255)                                       cctTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCt                                                                                           >  8:85129/1‑139 (MQ=255)                                                        aCCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCct                                                                             <  3:115249/136‑1 (MQ=255)                                                           aaTGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCctctc                                                                          >  3:295701/1‑136 (MQ=255)                                                           aaTGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCctctc                                                                          >  6:257418/1‑136 (MQ=255)                                                                  gtagagCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAg                                                                 >  7:29785/3‑138 (MQ=255)                                                                        cTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAg                                                             >  3:260695/1‑136 (MQ=255)                                                                        cTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCATCGCTTCAAATTCCTCTGTAGTCGCAGGAAg                                                             >  3:266221/1‑136 (MQ=255)                                                                            tGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGc                                                         <  3:111228/136‑1 (MQ=255)                                                                            tGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGc                                                         <  4:295701/136‑1 (MQ=255)                                                                              cGAATCATCCGAGTGATTTTTGCGCTTTTATTACTTTAAAATAAGTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCAtct                                                     <  1:255205/138‑1 (MQ=255)                                                                                    atcCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCaaa                                              <  7:312193/139‑1 (MQ=255)                                                                                           tGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCtttt                                       <  2:20596/139‑1 (MQ=255)                                                                                           tGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCtttt                                       >  8:135958/1‑139 (MQ=255)                                                                                                                 ttAAAATAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCtttt                   >  8:218601/1‑137 (MQ=255)                                                                                                                    aaaTAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCgg                                                          >  4:426616/1‑95 (MQ=255)                                                                                                                    aaaTAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCgg                                                          <  3:426616/95‑1 (MQ=255)                                                                                                                    aaaTAACTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCtttt                   >  6:397449/1‑134 (MQ=255)                                                                                                                          cTTTTAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGt        <  7:46884/139‑1 (MQ=255)                                                                                                                            tttAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTg         >  4:26327/1‑136 (MQ=255)                                                                                                                             ttAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTa       <  7:51948/137‑1 (MQ=255)                                                                                                                             ttAATCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTAGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTg         <  4:21293/135‑1 (MQ=255)                                                                                                                               aaacACTAAACATAGTTCCCTGTCGCCGAGAAGAAGGAAGAGAGCGGCCCTCGCTTCAAAATCCTCTCTAGTCGCAGGAAGCGGCAGCTCCTCAAATGCTTTTCTCATGTCAGCCAGTCTTTTCAGAAATTTGTAt      <  6:327975/133‑1 (MQ=255)                                                                                                                               aaTCACTAAACATTGTTCCATTTCGCCGCCAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTa       <  5:289712/135‑1 (MQ=255)                                                                                                                                ctCACTAAACATAGTACCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCGTTTCTCATGGCAGCAAGTCTTTTCAGAAATTTGTATg     <  4:260695/135‑1 (MQ=255)                                                                                                                                aTCACTAATCATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTATg     <  6:91497/136‑1 (MQ=255)                                                                                                                                aTCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTATg     <  4:300058/136‑1 (MQ=255)                                                                                                                                aTCACTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTATGCTg  <  7:218601/139‑1 (MQ=255)                                                                                                                                aTCAATAAACATTTTTCCAATTCGCCGAGAAGATGGAAGAGAGCGTCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTATg     <  4:266221/136‑1 (MQ=255)                                                                                                                                   aCTAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTATGCt   <  5:166643/135‑1 (MQ=255)                                                                                                                                     tAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCaaa                                                                                  <  7:424427/54‑1 (MQ=255)                                                                                                                                     tAAACATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCaaa                                                                                  >  8:424427/1‑54 (MQ=255)                                                                                                                                         |                                                                                                                                  CATGAAAATCGAACATAAGAAAAAACGCATAAGCTCCTCCTTTTTCATTAGGTTGACCAATGAAGGAAGAGCTCATGCGAATCATCCGAGTGATTTTTGCGCTTTTATTCCTTTAAAATAACTTTTAATCACTAAATATTGTTCCATTTCGCCGAGAAGATGGAAGAGAGCGGCCCTCGCTTCAAATTCCTCTCTAGTCGCAGGAAGCGGCATCTCCTCAAATTCTTTTCTCATGTCAGCAAGTCTTTTCAGAAATTTGTATGCTG  >  CP006881/2307320‑2307585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑