| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,801,996 | G→A | A327T (GCC→ACC) | U712_14440 → | UPF0118 membrane protein ytvI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,801,996 | 0 | G | A | 94.6% | 141.9 / ‑3.9 | 58 | A327T (GCC→ACC) | U712_14440 | UPF0118 membrane protein ytvI |
| Reads supporting (aligned to +/- strand): ref base G (1/1); major base A (21/32); minor base C (3/0); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.30e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCC > CP006881/2801863‑2802109 | aTCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTGCTTATTAAGCGGCAGCTGCCCGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCAGCTGGCGACACTCATCAcccgg > 1:425794/1‑137 (MQ=255) gATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTGTTTTTAAGCGGCAGCTGACAGAGCCCAACATACTAAGTAAATGTATCGGGGTCGTGCCGCTGGGAACACGCATCAcccgg > 5:224092/1‑134 (MQ=255) ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc < 7:363988/139‑1 (MQ=255) ggCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCt < 7:22545/139‑1 (MQ=255) tGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTa > 5:174808/1‑135 (MQ=255) aaGCATTCGTAAATGGTATCCTGTACCTCGTCGTTCTTTTTCAGCGGGAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCTACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 7:121391/139‑1 (MQ=255) aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAattatt < 4:84911/136‑1 (MQ=255) aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 2:33363/139‑1 (MQ=255) tCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTAAGCGGCAGCTGACAGAGCCCCAGATACTAAGTAAATCTATCGGGATCGATACGCTGGCAACACACATCACCCATGTTGCCGGCTTTTAAATTTTAGGGttttt > 1:166493/1‑139 (MQ=255) tCGGAATTGGTATCCTGTACCTCGTCGTTCATATTCAGCGGCAGCTGACAAAGCCCAAGATACTAAGTAAATCTATCTGGATCGATCCGCTGGCACCCCACATCACCCTGTTTGCCGGCTTTAAATTATTCGGGttttt > 8:421827/1‑139 (MQ=255) tttgTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACt < 7:255294/136‑1 (MQ=255) ttGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTTAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACt < 7:143891/139‑1 (MQ=255) ttGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAgg < 6:224092/136‑1 (MQ=255) gaTCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGAtt < 1:83883/138‑1 (MQ=255) tCCTGTACCTCGTCGTTCTTATTCAGCTGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGTATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGAt < 3:326222/136‑1 (MQ=255) cTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGC‑TTAAATTATTCGGGTTTTTAGGACTGATTGCggg < 8:78894/139‑1 (MQ=255) tACCTCGTCGTTCTTTTTCAGCGGCAGCAGACAGAGCCCAAGATACTAAGTAAATATATAGGGATCGATCCCCAGGCAACACAGCTCACCCTGTTTGACGGCTTTAAAATATTAGGGTTTTTAGGAATGATTGCCGGAc > 8:255294/1‑139 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGc < 6:140263/134‑1 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg < 1:43578/137‑1 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGAAAGAGCCCAAGATACAAAGTAAATCTAAAGGGATCCATCCGCAGGCAACACTCCTCACCCTGTTTGCCGGCTTTAAATAAATAGGGTTTTTAAGACTAATTgatgaac > 8:143891/1‑133 (MQ=255) tACCTCGGCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCgg < 6:374097/136‑1 (MQ=255) cTCGTCGTTGTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGGTGGCTGCGCGGGCAACACTCATCCCCCGGGTTGGCGGGGTTAAATTATTCGGGTTTTTAGGTCGGTTTGCggggc > 6:306449/1‑134 (MQ=255) cTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATATATCGGGATCGATCCGCTGGCAACACTCATCACCCAGGTTGCCGGCTTTAAATTATTAGGGTTTTTTGGACTGATTGCgggcc > 5:117292/1‑134 (MQ=255) cgtcgtTCTTATTCAGCGGCAGCGGACCGAGCCCCAGATACTAAGTAAATCTATCGGGCTCGCTCCGCTGGCAACACTCATCCCCCAGTTTGCCGGCTTTAAATTATTAGGGTTTTTAGGACTGATTTCGGGCCCGGcc > 2:83883/1‑138 (MQ=255) cgtcgtGCTTATTAAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATAGGGATAGATCCGCTGGCAACACTCATCCCCCAGTTTGCCGGCGTTAAATTATTAGGGTTTTTTGGACTGATTGCGGGGCCGGCt > 8:266656/1‑139 (MQ=255) agtcgtTGTTATTAAGCGGCACCGGACATAGCCCAAGCTACTAATTAAATGTATCTTGATCGATCCGCTTGCAACACTCATGGACCTGGTTTCCGGCTTTAAATTTATCGGGTTTTTTTGACTGATTGCGGGACCGGCt > 2:345147/2‑139 (MQ=255) cgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTa < 2:108687/139‑1 (MQ=255) aTTCAGCGGCATCTGTCAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCTGCTTTATATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTc < 5:292021/135‑1 (MQ=255) aTTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc < 2:425794/138‑1 (MQ=255) ttCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtcat < 1:329159/139‑1 (MQ=255) aaGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATATATAGGGATCGAACCGCTGGCAACACTCATAACCCAGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc > 5:217261/2‑135 (MQ=255) cGGCAGCTGACAGAGCCCAATATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTCTTTGCCGGCTTTAAATTATTAGGTTTTATAGGACTGATTCCGTGACCGGCTGTACTAGTCatcatc > 8:311836/1‑138 (MQ=255) cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCatcatc > 2:49921/1‑138 (MQ=255) cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCatcatc > 2:375802/1‑138 (MQ=255) cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCatcatc > 7:111472/1‑138 (MQ=255) ccAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcatca > 3:252238/1‑135 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAgg > 2:240101/1‑133 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAgg < 1:240101/133‑1 (MQ=255) aGTAAATCTATCGGGATCGATCCGCTGGCAAGACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGGCATCATCCAAGCTTTCATCACAACAGGCGCATTaa < 7:51276/139‑1 (MQ=255) aGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt < 6:217261/136‑1 (MQ=255) aGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa < 7:359028/139‑1 (MQ=255) gTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt > 3:213516/1‑135 (MQ=255) tCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGaaa > 1:21549/1‑139 (MQ=255) tATCGGGATCGATCCGCTGTCAACACTCATCACCCTTTTTGCAGTCTTTAAATTATTCGGTTTTTTAGGACTGATTTATGGTCCGGTTTTATTATTTATCATACAAGATTTCATCACAACAGGCGCATTAAAAGaa < 6:170160/136‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGaa < 6:174808/134‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 7:266656/137‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 1:375802/137‑1 (MQ=255) cGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg < 2:142737/139‑1 (MQ=255) gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 4:213516/135‑1 (MQ=255) ggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCGGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGGTTTCCTCACAACAGGCGCATTAAAAGAAATATg > 4:391817/1‑136 (MQ=255) ggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc < 3:193411/78‑1 (MQ=255) ggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc > 4:193411/1‑78 (MQ=255) ggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGTCTGTACTCGTCATCATCCAAGCGTTCATCACAACAGGCGCATTAAAAGAAATATg < 3:180538/136‑1 (MQ=255) ggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATg < 4:252238/136‑1 (MQ=255) ggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGGTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTc < 2:214957/139‑1 (MQ=255) gatcCGCTGGCAACACTCATCACCCTGTTTGACGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCAAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTcc > 4:36929/1‑135 (MQ=255) aTCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTc < 7:407561/116‑1 (MQ=255) aTCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTc > 8:407561/1‑116 (MQ=255) | ATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCC > CP006881/2801863‑2802109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |