Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,724,542 A→G S95S (TCT→TCC)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,5420AG98.8% 309.2 / NA 84S95S (TCT→TCC) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (42/41);  minor base T (1/0);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTT  >  CP006881/3724407‑3724664
                                                                                                                                       |                                                                                                                          
tGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGa                                                                                                                          >  2:301150/1‑138 (MQ=255)
     ctTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                                                                                    >  1:204390/1‑139 (MQ=255)
      ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                     <  8:32855/137‑1 (MQ=255)
      ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                     >  8:317585/1‑137 (MQ=255)
      ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                     <  7:317585/137‑1 (MQ=255)
       tttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                     >  5:308231/1‑136 (MQ=255)
         tACATGTCCGATCGCCCACAGGCCGATCTCATATTGTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCCCCAGCAGCCCATTTTGAATCCTTGCGGTCGCAAAGGCCAAAGGGTCGGATAGcgcg                                                                                                                   >  6:277926/1‑136 (MQ=255)
         tACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGTACAAATGGTCGGAAAgcgc                                                                                                                    >  4:381192/1‑135 (MQ=255)
         tACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                                                                                    <  5:321114/135‑1 (MQ=255)
         tACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                                                                                    >  4:178081/1‑135 (MQ=255)
              gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGATCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCTATCGCAAAGGAAAAATCGTCGGAAAGCGCGATATGAAt                                                                                                           >  2:418948/1‑139 (MQ=255)
              gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                                                                                           >  2:49530/1‑139 (MQ=255)
                 cGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                                                                                    <  3:123900/127‑1 (MQ=255)
                 cGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                                                                                    >  4:123900/1‑127 (MQ=255)
                 cGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCATTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                                                                                           >  4:275560/1‑136 (MQ=255)
                  gATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGa                                                                                                       <  1:83578/139‑1 (MQ=255)
                              gCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAgc                                                                                              >  3:14994/1‑136 (MQ=255)
                              gCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGAAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAgcggc                                                                                           >  2:427564/1‑139 (MQ=255)
                                  aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                                                                                       >  7:139217/1‑139 (MQ=255)
                                     tcatATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGaaa                                                                                                                        >  6:329805/4‑103 (MQ=255)
                                     tcatATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGaaa                                                                                                                        <  5:329805/100‑1 (MQ=255)
                                     tcatATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                                                                                       >  3:206298/4‑136 (MQ=255)
                                       atATTCTTTTTTATAGTGCGCCGTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGACTCCTTTCGATCGCAAAGGACAAATGGGCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccgc                                                                                      <  3:128618/134‑1 (MQ=255)
                                       atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccgc                                                                                      <  3:178081/134‑1 (MQ=255)
                                          ttCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAg                                                                                   <  4:14994/135‑1 (MQ=255)
                                           tCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCt                                                                                 >  6:252801/1‑136 (MQ=255)
                                             ttttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCTGAAAGCGCGATATGAATGTGATCGATCAGCGTCGCCGCGAGCTCTCCt                                                                            >  2:201066/1‑139 (MQ=255)
                                               ttttATAGAGCGCCTTTCTCTCATGCAGCAATATATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTc                                                                                <  6:364537/133‑1 (MQ=255)
                                               ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctcc                                                                             <  8:139217/136‑1 (MQ=255)
                                               ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTc                                                                                <  5:76257/133‑1 (MQ=255)
                                               ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTc                                                                          >  8:280649/1‑139 (MQ=255)
                                                    tatcgcgcCTTTATCTCATTCAGCAATTTATTCTGCACAAGCAGCCAATTTTGAATCATTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                      <  1:418948/134‑1 (MQ=255)
                                                    taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTcc                                                                         <  3:165912/135‑1 (MQ=255)
                                                    taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                      <  1:108036/138‑1 (MQ=255)
                                                    taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCAt                                                                     <  1:49530/139‑1 (MQ=255)
                                                       agCGCCTTTATCTCATGCAGCAATTTATTCTTCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAACGGACAAATGGTCGGAAAGCGCGATATGAAGGTGAGCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                                  <  8:302096/139‑1 (MQ=255)
                                                       agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCATAGGACAAATTGTCGGAAAGCTATATATGAATTTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCAt                                                                     <  6:346678/136‑1 (MQ=255)
                                                       agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                                  <  2:204390/139‑1 (MQ=255)
                                                         cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAg                                                                   <  4:206298/136‑1 (MQ=255)
                                                         cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATCAATCTGATCGCTCAGCGCCGCCGCGAGCTCTCCTACCCCATAg                                                                   >  1:50329/1‑136 (MQ=255)
                                                            ctttATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGTTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgatg                                                             <  1:201066/138‑1 (MQ=255)
                                                            ctttATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgc                                                                                                                      >  5:399918/2‑82 (MQ=255)
                                                            ctttATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgc                                                                                                                      <  6:399918/81‑1 (MQ=255)
                                                               tATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGAGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgat                                                              <  3:275560/135‑1 (MQ=255)
                                                                     aTGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtg                                                                >  1:82141/1‑127 (MQ=255)
                                                                     aTGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtg                                                                <  2:82141/127‑1 (MQ=255)
                                                                     aTGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTgatgat                                                           <  5:317779/132‑1 (MQ=255)
                                                                     aTGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGAGCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATc                                                        <  1:301150/135‑1 (MQ=255)
                                                                      tGCAGCAATTTATTCTTCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATTAATGTGATCGCTCAGCGGCGCcgcg                                                                                     <  8:14001/105‑1 (MQ=255)
                                                                      tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCcgcg                                                                                     >  7:14001/1‑105 (MQ=255)
                                                                      tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                      >  1:366693/1‑120 (MQ=255)
                                                                      tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                      <  2:366693/120‑1 (MQ=255)
                                                                      tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTgatgat                                                           <  7:433208/131‑1 (MQ=255)
                                                                      tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTgatgat                                                           >  8:433208/1‑131 (MQ=255)
                                                                      tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                   >  1:118795/1‑139 (MQ=255)
                                                                      tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                   >  1:265150/1‑139 (MQ=255)
                                                                      tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                   >  2:731/1‑139 (MQ=255)
                                                                         agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                   >  5:92555/1‑136 (MQ=255)
                                                                         agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                   >  5:85588/1‑136 (MQ=255)
                                                                         agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                   >  6:394821/1‑136 (MQ=255)
                                                                         agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                                <  1:731/139‑1 (MQ=255)
                                                                         agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCAGATTATATCCTCat                                                   >  6:141970/1‑135 (MQ=255)
                                                                         agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGGCGGAAAGCGCGATAGGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                   <  6:362064/136‑1 (MQ=255)
                                                                                   tCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGa                                                                                                                          <  7:225237/55‑1 (MQ=255)
                                                                                   tCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGa                                                                                                                          >  8:225237/1‑55 (MQ=255)
                                                                                       cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGACAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGc                                     <  1:154788/136‑1 (MQ=255)
                                                                                       cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCtata                                        <  5:298291/133‑1 (MQ=255)
                                                                                       cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATg                                      <  5:141970/135‑1 (MQ=255)
                                                                                       cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATg                                      <  4:246011/135‑1 (MQ=255)
                                                                                       cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctcc                                  >  7:314960/1‑139 (MQ=255)
                                                                                       cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc                                   >  8:119319/1‑138 (MQ=255)
                                                                                       cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCGATTTGTATATGctcc                                  >  7:272427/1‑139 (MQ=255)
                                                                                       cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTAATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctcc                                  >  1:283869/1‑139 (MQ=255)
                                                                                             cagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGc                            <  2:265150/139‑1 (MQ=255)
                                                                                               gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTcc                                                                         >  3:225678/1‑92 (MQ=255)
                                                                                               gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTcc                                                                         <  4:225678/92‑1 (MQ=255)
                                                                                               gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGATCCTCCgg                             >  6:9932/1‑136 (MQ=255)
                                                                                                      ttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAgtgt                       <  3:24658/135‑1 (MQ=255)
                                                                                                      ttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAgtgt                       <  6:216463/135‑1 (MQ=255)
                                                                                                          aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGa               >  8:372333/1‑139 (MQ=255)
                                                                                                                 cGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTg           >  6:107118/1‑136 (MQ=255)
                                                                                                                          aGGACAAATGGTCGGAAAGCGCGATATGAATGTTATCGCTCAGCGGCGCCGCGAGCTCTACTTCCGCATATCTTATGATATCCTCAGCTAATTCTATATGATCATCAGGCATTGTTTGCAGGATTTGTTTTAACtt  >  4:156600/1‑136 (MQ=255)
                                                                                                                                 aTGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctcc                                                                             >  2:251310/1‑54 (MQ=255)
                                                                                                                                 aTGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctcc                                                                             <  1:251310/54‑1 (MQ=255)
                                                                                                                                       |                                                                                                                          
TGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTT  >  CP006881/3724407‑3724664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑