| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,239 | 2 bp→AA | intergenic (‑71/‑457) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,239 | 0 | G | A | 100.0% | 158.4 / NA | 48 | intergenic (‑71/‑458) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (35/13); total (35/13) | |||||||||||
| * | CP006881 | 3,725,240 | 0 | G | A | 93.6% | 139.9 / ‑6.4 | 48 | intergenic (‑72/‑457) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (35/9); minor base T (0/3); total (35/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑TTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAG > CP006881/3725119‑3725366 || ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCATCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 8:239090/1‑139 (MQ=255) atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 5:214303/1‑136 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAgttcttt > 7:265628/1‑139 (MQ=255) caAAAAGACCATCCACACCCGAAAAGATACAACCAAACGTTGCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCA‑GC‑CTTTTT‑A‑GCCGCCCGTTTTCTCCGCCCCACCCCGCAAGAAAACACGGCCGAtttc < 4:185941/136‑1 (MQ=255) aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttgttttct > 1:129917/1‑139 (MQ=255) aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttt > 5:145792/1‑136 (MQ=255) ccATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGGTTTCTCAGCCGCATCCCGCAAGAAAAGACGTCCGAtttgttttggagca > 8:384754/1‑132 (MQ=255) tCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCa > 5:308901/1‑135 (MQ=255) cccGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGc > 8:317759/1‑139 (MQ=255) accacggATATATGCAAAGGTTTATTATAAAAAATACCCTGCGTTTTTTTTTGGCCGGCAGGA‑T‑TTTT‑A‑GCTGCCCGTTTTCTCTCCCGCTCCGCGCATCAAACCTCGGCCGATTTCTTTTCTCGCAAACTAGCGGctc < 8:326442/133‑1 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAACCATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGTCCGATGTCGTTTCTCGCAAACTAGCCGcgc > 7:27174/1‑135 (MQ=255) aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc > 3:178612/1‑134 (MQ=255) aTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAg > 5:413402/1‑135 (MQ=255) aCAACCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGTCAGGCA‑GC‑CTTTTT‑T‑GCAGCCCGTTTTCACAGCCGCAGCCCCCAAGAAAAGACGGCCGAATTATTTTCTCGGAAACTAGCGGCTCATGCaaaa > 7:9713/1‑138 (MQ=255) cAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCat > 4:116548/1‑134 (MQ=255) aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATCGCAAACTAGCGGCTATAGCAAAAc > 4:211782/1‑136 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:317759/139‑1 (MQ=255) ccAAACGTTTCAAACAAAAACATCCCCCCGCTTCTTTTGGGCAGGCC‑GC‑CTTTTT‑C‑GCCGCCCGTTTTCTCCCCCCCCCCCCGAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 2:283620/138‑1 (MQ=255) acaCGTTGAATACAAAAACAGCCCCCCGCTTTTTTTGGGCCGGCA‑GCTT‑TTTT‑C‑GCCGCCGGTTTTCGCCGCCGCCGCCCCCACCAAACCACGGCCGATTTCTTTTCTCGAAAACTAGCGGCTCTAGCAAAACTCa < 6:308901/134‑1 (MQ=255) aaaCTTTTCATAAAAAAACAGCCCTCCGCTTCTTTTTGGCAGGCC‑GCGT‑TTTT‑A‑GCCGCCCGTTTACTCAGCCGCAGCCCGAAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa < 3:211782/136‑1 (MQ=255) aaaCGTTTCATACAAAACCATCCATCCGGTGATTTTTGGCCCGCA‑GC‑CTTTTT‑C‑GCAGCCGTTTTTCTCAGCCGCCCCCCGCAACAAACCACGGCCGTTTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa < 6:413402/136‑1 (MQ=255) ccttttAATATAAAAACATCCCTCCGTTTGTTTTTGGCTGGCA‑GC‑T‑TTTTAA‑GCTCCCGTTTTTCTCAGCCGCCGCCCCAATTAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATg < 6:214303/133‑1 (MQ=255) ccGTTTAATAGAACAACACCCCTCCGGTTCTTTTGGCCAGGCA‑GC‑C‑TTTT‑ACCCTCCCCTTTTTCTCCGCCGCCGCCCCAAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAACCTCATGAct < 8:119151/138‑1 (MQ=255) aggTTTAATACAAAAACATCCCTCGGCTTATTTTGGGCAGGCA‑GCTT‑TTTT‑C‑GCTGCCCGTTTTCTCAGCCGCAGCCCGCATGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 7:239090/137‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTGTCGCAAACAAGCGGCTCTAGCAAAACTccagtc > 7:326442/1‑133 (MQ=255) aCGTTTAATAGAAAAACACCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑C‑GCAGCCCGTTTTCTCCCCCGCAGCCCCCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 7:229833/139‑1 (MQ=255) aCGTTACATACAAAACCCTCCCTCCGCTTCTTTTTGGCAGCCC‑GCAT‑TTTT‑C‑GCCCCCCTTTTCCTCCGCCGCCCCCCGAAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 2:139459/139‑1 (MQ=255) tttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct > 3:216040/1‑136 (MQ=255) tttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTATCGCAAACTAGCGGCTCTAGCAAAACTCATGAct > 4:169773/1‑136 (MQ=255) tttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCACGCC‑GC‑CTTTTT‑A‑TCAGCCCGTTTTCTCAGCCGCAGCCCTCAACAAAAGACGGAGGAATTCTTTTATCGAAATCTGTCCGCTCGAGCAAAAATCATGCct > 6:51714/1‑136 (MQ=255) tCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCCAACTAGCGGCTCGAGCAAAACTGATGACGCTATg > 2:54646/1‑139 (MQ=255) tAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCGTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAcgagat > 6:315501/1‑130 (MQ=255) aaaaaCATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGAGTTCGTTTGTCGCACACTAGCGGCTGTAGCAAAACTCATGGCTGTTTGTTAGAc > 2:301827/1‑139 (MQ=255) aaCATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAc > 6:303633/1‑136 (MQ=255) cccTCCGCTTCTTTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTCTCGCCAACTAGCGGGACTAGGAAAACTCATGACTCAATGATAGCCCGTGGaaa > 8:223844/1‑139 (MQ=255) cttTTTGGCAGGCA‑GC‑CTTTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATTGACCGTGGAAAGAGaaa > 3:388116/1‑134 (MQ=255) tttGGCAGGCA‑GC‑CTTTTT‑A‑GAAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGGTCTAGCAAAACTCAAGACTCTATGATATAACCTGGAAAGAGAATTCCTTGt > 8:192367/1‑139 (MQ=255) ttGGCAGGCT‑GC‑CTTTTT‑A‑GCACCCCGTTTTCTCTGCCGCAGCCCCAAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCtt < 4:388116/135‑1 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGCCTCTATGATAGTCCGTAGAAAGAGCATTGGTTGTGCCAATATGAGTGAAAAAAcc > 1:173660/1‑138 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCGCGCCAACTAGCGGCTCTAGCAAACCTCATGCCTCTATGATAGACCGTGGAAAGGGAATTAGTTGTCACAATATGAGTTaaaaac > 4:313/1‑135 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCaa > 7:314573/1‑139 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCa > 1:141905/1‑138 (MQ=255) ccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTGTCGCAAACTAGCGGCTCTAGCAAAACTCCTGACTCTATGATAGACCGGGGAAAGAGAATTCTTTGGGACCATATTAGGGAAAAAAcc > 4:5245/1‑135 (MQ=255) gTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCTTGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc > 5:175426/1‑136 (MQ=255) gTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGCCTCGATGATAGACCGGGGAAAGAGAATTCTTTGTCAGAATATGAGTGATAAAACCGAc > 3:280245/1‑136 (MQ=255) gTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc > 3:425342/1‑136 (MQ=255) cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTTTGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGGTTTTAGAAg > 4:370704/1‑136 (MQ=255) cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCCCAAAAGTGCGGCTCTAGCAAAACTGATGACTCGATGATAGTCCCTGGAAAGAGAATTATTTGTGAACAAATTACTGAAAAAAACCACGACTGTTtagtag > 4:63259/1‑136 (MQ=255) || TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GC‑C‑TTTT‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAG > CP006881/3725119‑3725366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |