Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,948 C→T 100% P254P (CCC→CCT)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,9480CT100.0% 192.6 / ‑6.6 57P254P (CCC→CCT) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (38/18);  minor base A (1/0);  total (39/18)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACT  >  CP006881/3723815‑3724079
                                                                                                                                     |                                                                                                                                   
ccATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGatc                                                                                                                                 >  7:89785/1‑137 (MQ=255)
       gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCGGGATGTACTACACACTGAATCCTGAgcaataaa                                                                                                                           <  7:127757/136‑1 (MQ=255)
               tCGCGCACCCTGATACAGTGACAATGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCGCGGCAAGCTGACCGCGGGAATCGTTATGATGGGCTGGATGAACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                                                                                                                 <  1:201700/139‑1 (MQ=255)
               gcGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                                                                                                                 <  2:78679/138‑1 (MQ=255)
                  aGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATCTACTACACACTGAATCCTGATCAATAAAAAAACTTCCaga                                                                                                               >  6:169732/1‑138 (MQ=255)
                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt                                                                                                        <  8:6029/130‑1 (MQ=255)
                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt                                                                                                        >  7:6029/1‑130 (MQ=255)
                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                                               >  8:27884/1‑139 (MQ=255)
                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                                               >  1:46293/1‑139 (MQ=255)
                                    cactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTc                                                                                             >  1:189525/6‑138 (MQ=255)
                                     actgaTGGGAGCCTTCAGAAACTTAATCCCCGTAACGCGCGGCAAACTGACCGCGGGAATAGTTATGTTGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                           <  4:51037/135‑1 (MQ=255)
                                     actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                           <  5:254941/135‑1 (MQ=255)
                                     actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAAAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                           <  8:89785/135‑1 (MQ=255)
                                     actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACAATGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCATGCAAGTCa                                                                                            >  3:253728/5‑138 (MQ=255)
                                     actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGGACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                                            >  5:239071/5‑138 (MQ=255)
                                     actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAAGAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATATAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                                            >  2:27315/5‑138 (MQ=255)
                                         aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGCCCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg                                                                                       >  2:76914/1‑139 (MQ=255)
                                           gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                                     >  7:227797/1‑139 (MQ=255)
                                             gAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTa                                                                                   >  5:203294/1‑139 (MQ=255)
                                              aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTTTGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTa                                                                                   >  1:92139/1‑138 (MQ=255)
                                              aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                                                                                  >  3:276312/1‑139 (MQ=255)
                                              aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                                                                                  >  5:16172/1‑139 (MQ=255)
                                                   tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGCTCACTCCCTGCAAGTCAAAGCGGTTAGTcc                                                                               >  4:156894/1‑137 (MQ=255)
                                                     acaaaCTTAATCCCCGTTCCGCGCGGCAATCTGACCGCGGTAATAGTTATGATGGGCTGGAGGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                            <  6:16172/136‑1 (MQ=255)
                                                     aGAAAATTAATCCCCGTTCCGCGCGGAATTCTGACCGCGGGAATAGTTATGATGGGCTGGATTTACTACACACTCAATGATTATAAATAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                            <  2:92139/138‑1 (MQ=255)
                                                      gAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgagag                                                                          <  7:27884/139‑1 (MQ=255)
                                                             aaTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACGTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTg                                                                    >  3:94890/1‑138 (MQ=255)
                                                                 cccTTTACTCTCGGAAATCTGACCGCGTTAATATTTATTATGTGCTGTATGTACTACACACTTATTCATTATAAATAAAAAAACTTAAAGATATCATTCCATGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                               <  4:276312/139‑1 (MQ=255)
                                                                   cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc                                                             >  7:282968/1‑139 (MQ=255)
                                                                   cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGATATTCCGAGAGATCTTGAAGACGc                                                             >  1:224580/1‑139 (MQ=255)
                                                                   cGTTACGCTCGGCAATCTGACCGCGGGAAAAGTTATGATGGGATGGATGTACTACACACTGAATCCTGATAAATAAAAAAACTTCCAGAGATCACTCCATGCAAGTCAAAGCGGTTATTACTAGAGATCTTGAAGACGc                                                             >  8:26266/1‑139 (MQ=255)
                                                                              gCAATCTGAACGCGGTAATTGTAATGATGGGCTGTATGTAATACTCTCTGTATCCTAATCAATAAAAACACTTCCAGATATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGATGGACATGCAg                                                  <  6:202786/139‑1 (MQ=255)
                                                                                                     atgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGatt                           >  8:286413/1‑137 (MQ=255)
                                                                                                        atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCg                        >  3:16147/1‑139 (MQ=255)
                                                                                                           ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc                      <  8:282968/138‑1 (MQ=255)
                                                                                                             cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa                   >  7:70921/1‑139 (MQ=255)
                                                                                                                       tACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGa          >  6:168492/1‑138 (MQ=255)
                                                                                                                          acactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATAtt      >  6:63452/7‑139 (MQ=255)
                                                                                                                           cactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATAtt      >  8:216057/6‑138 (MQ=255)
                                                                                                                            actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa    >  8:12306/5‑139 (MQ=255)
                                                                                                                            actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa    >  2:33227/5‑139 (MQ=255)
                                                                                                                            actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa    >  1:96927/5‑139 (MQ=255)
                                                                                                                            actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa    >  2:121209/5‑139 (MQ=255)
                                                                                                                              tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                           <  2:107712/48‑1 (MQ=255)
                                                                                                                              tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                           >  1:107712/3‑50 (MQ=255)
                                                                                                                              tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaa                    <  7:42371/119‑1 (MQ=255)
                                                                                                                              tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaa                    >  8:42371/3‑121 (MQ=255)
                                                                                                                              tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt  >  8:64135/3‑137 (MQ=255)
                                                                                                                              tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt  >  8:87247/3‑137 (MQ=255)
                                                                                                                              tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt  >  8:32763/3‑137 (MQ=255)
                                                                                                                              tgaaTCCAGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt  >  5:121117/3‑137 (MQ=255)
                                                                                                                                aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                                     >  1:160668/1‑86 (MQ=255)
                                                                                                                                aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                                     <  2:160668/86‑1 (MQ=255)
                                                                                                                                aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                                  <  8:133943/89‑1 (MQ=255)
                                                                                                                                aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                                  >  1:171488/1‑89 (MQ=255)
                                                                                                                                aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                                  >  7:133943/1‑89 (MQ=255)
                                                                                                                                aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                                  <  2:171488/89‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACT  >  CP006881/3723815‑3724079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑