Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,952 A→C 100% K256Q (AAA→CAA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,9520AC97.3% 207.2 / ‑4.5 61K256Q (AAA→CAA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (42/16);  total (43/18)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAA  >  CP006881/3723822‑3724086
                                                                                                                                  |                                                                                                                                      
gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCGGGATGTACTACACACTGAATCCTGAgcaataaa                                                                                                                                  <  7:127757/136‑1 (MQ=255)
        tCGCGCACCCTGATACAGTGACAATGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCGCGGCAAGCTGACCGCGGGAATCGTTATGATGGGCTGGATGAACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                                                                                                                        <  1:201700/139‑1 (MQ=255)
        gcGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                                                                                                                        <  2:78679/138‑1 (MQ=255)
           aGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATCTACTACACACTGAATCCTGATCAATAAAAAAACTTCCaga                                                                                                                      >  6:169732/1‑138 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt                                                                                                               <  8:6029/130‑1 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt                                                                                                               >  7:6029/1‑130 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                                                      >  8:27884/1‑139 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                                                      >  1:46293/1‑139 (MQ=255)
                             cactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTc                                                                                                    >  1:189525/6‑138 (MQ=255)
                              actgaTGGGAGCCTTCAGAAACTTAATCCCCGTAACGCGCGGCAAACTGACCGCGGGAATAGTTATGTTGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                                  <  4:51037/135‑1 (MQ=255)
                              actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                                  <  5:254941/135‑1 (MQ=255)
                              actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAAAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                                  <  8:89785/135‑1 (MQ=255)
                              actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACAATGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCATGCAAGTCa                                                                                                   >  3:253728/5‑138 (MQ=255)
                              actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGGACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                                                   >  5:239071/5‑138 (MQ=255)
                              actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAAGAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATATAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                                                   >  2:27315/5‑138 (MQ=255)
                                  aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGCCCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg                                                                                              >  2:76914/1‑139 (MQ=255)
                                    gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                                            >  7:227797/1‑139 (MQ=255)
                                      gAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTa                                                                                          >  5:203294/1‑139 (MQ=255)
                                       aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTTTGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTa                                                                                          >  1:92139/1‑138 (MQ=255)
                                       aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                                                                                         >  3:276312/1‑139 (MQ=255)
                                       aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                                                                                         >  5:16172/1‑139 (MQ=255)
                                            tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGCTCACTCCCTGCAAGTCAAAGCGGTTAGTcc                                                                                      >  4:156894/1‑137 (MQ=255)
                                              acaaaCTTAATCCCCGTTCCGCGCGGCAATCTGACCGCGGTAATAGTTATGATGGGCTGGAGGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                                   <  6:16172/136‑1 (MQ=255)
                                              aGAAAATTAATCCCCGTTCCGCGCGGAATTCTGACCGCGGGAATAGTTATGATGGGCTGGATTTACTACACACTCAATGATTATAAATAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                                   <  2:92139/138‑1 (MQ=255)
                                               gAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgagag                                                                                 <  7:27884/139‑1 (MQ=255)
                                                      aaTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACGTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTg                                                                           >  3:94890/1‑138 (MQ=255)
                                                          cccTTTACTCTCGGAAATCTGACCGCGTTAATATTTATTATGTGCTGTATGTACTACACACTTATTCATTATAAATAAAAAAACTTAAAGATATCATTCCATGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                                      <  4:276312/139‑1 (MQ=255)
                                                            cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc                                                                    >  7:282968/1‑139 (MQ=255)
                                                            cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGATATTCCGAGAGATCTTGAAGACGc                                                                    >  1:224580/1‑139 (MQ=255)
                                                            cGTTACGCTCGGCAATCTGACCGCGGGAAAAGTTATGATGGGATGGATGTACTACACACTGAATCCTGATAAATAAAAAAACTTCCAGAGATCACTCCATGCAAGTCAAAGCGGTTATTACTAGAGATCTTGAAGACGc                                                                    >  8:26266/1‑139 (MQ=255)
                                                                       gCAATCTGAACGCGGTAATTGTAATGATGGGCTGTATGTAATACTCTCTGTATCCTAATCAATAAAAACACTTCCAGATATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGATGGACATGCAg                                                         <  6:202786/139‑1 (MQ=255)
                                                                                              atgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGatt                                  >  8:286413/1‑137 (MQ=255)
                                                                                                 atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCg                               >  3:16147/1‑139 (MQ=255)
                                                                                                    ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc                             <  8:282968/138‑1 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa                          >  7:70921/1‑139 (MQ=255)
                                                                                                                tACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGa                 >  6:168492/1‑138 (MQ=255)
                                                                                                                   acactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATAtt             >  6:63452/7‑139 (MQ=255)
                                                                                                                    cactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATAtt             >  8:216057/6‑138 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa           >  2:33227/5‑139 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa           >  1:96927/5‑139 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa           >  2:121209/5‑139 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa           >  8:12306/5‑139 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                                  >  1:107712/3‑50 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                                  <  2:107712/48‑1 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaa                           >  8:42371/3‑121 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaa                           <  7:42371/119‑1 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  8:32763/3‑137 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  8:64135/3‑137 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  8:87247/3‑137 (MQ=255)
                                                                                                                       tgaaTCCAGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  5:121117/3‑137 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                                            <  2:160668/86‑1 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                                            >  1:160668/1‑86 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                                         <  2:171488/89‑1 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                                         >  1:171488/1‑89 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                                         <  8:133943/89‑1 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                                         >  7:133943/1‑89 (MQ=255)
                                                                                                                              tgATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGAGTCCGGCAAATGTAATTGATATTCATTTTTcta   >  6:281765/2‑136 (MQ=255)
                                                                                                                               gATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                                   <  8:213319/57‑1 (MQ=255)
                                                                                                                               gATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                                   >  7:213319/1‑57 (MQ=255)
                                                                                                                               gATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  6:166499/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  5:101717/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  2:165140/1‑138 (MQ=255)
                                                                                                                                  |                                                                                                                                      
ACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAA  >  CP006881/3723822‑3724086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑