Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,392 A→C 100% L145L (CTT→CTG)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,3920AC97.8% 333.4 / ‑1.8 89L145L (CTT→CTG) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (49/38);  total (50/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCA  >  CP006881/3724259‑3724527
                                                                                                                                     |                                                                                                                                       
gATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAgaga                                                                                                                                    <  5:281765/139‑1 (MQ=255)
     aaGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGc                                                                                                                               >  5:228573/1‑139 (MQ=255)
        tATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCa                                                                                                                            <  6:168800/139‑1 (MQ=255)
         aTTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCa                                                                                                                            <  2:117688/138‑1 (MQ=255)
            gTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATg                                                                                                                         <  4:248063/138‑1 (MQ=255)
             ttttATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        <  4:135988/135‑1 (MQ=255)
               ttATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCa                                                                                                                            <  4:74112/131‑1 (MQ=255)
               ttATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCa                                                                                                                            >  3:74112/2‑132 (MQ=255)
                  ttttCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCttt                                                                                                                  >  5:111384/1‑139 (MQ=255)
                  ttttCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCttt                                                                                                                  >  2:80276/1‑139 (MQ=255)
                              tttCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGAt                                                                                                      <  5:262179/139‑1 (MQ=255)
                              tttCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGAt                                                                                                      <  6:228573/139‑1 (MQ=255)
                                                       cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGTCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCt                                                                             >  8:276485/1‑139 (MQ=255)
                                                       cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        <  8:242097/96‑1 (MQ=255)
                                                       cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        >  7:242097/1‑96 (MQ=255)
                                                       cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         <  1:167699/127‑1 (MQ=255)
                                                       cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCg                                                                                         >  2:167699/1‑127 (MQ=255)
                                                       cAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCt                                                                             >  4:8114/1‑139 (MQ=255)
                                                          cgcTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                               <  5:72570/134‑1 (MQ=255)
                                                          cgcTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                               >  6:72570/1‑134 (MQ=255)
                                                            cTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCttttt                                                                         >  8:65052/1‑138 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACg                                                                                                                                <  5:125265/80‑1 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACg                                                                                                                                >  6:125265/1‑80 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                               >  6:237726/1‑129 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                               <  5:237726/129‑1 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTtata                                                                     >  1:245673/1‑139 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTta                                                                       >  3:117428/1‑137 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTta                                                                       >  4:240823/1‑137 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTta                                                                       >  6:75987/1‑137 (MQ=255)
                                                               aaTACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTta                                                                       >  6:16437/1‑137 (MQ=255)
                                                                   cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  2:245673/138‑1 (MQ=255)
                                                                   cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  7:276485/138‑1 (MQ=255)
                                                                   cATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  3:140193/138‑1 (MQ=255)
                                                                       ctctCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  6:67699/134‑1 (MQ=255)
                                                                       ctctCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  5:67699/1‑134 (MQ=255)
                                                                         ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGCTCTCATATTCTTTTTTATAGAGCGCcttt                                                           >  8:108600/1‑139 (MQ=255)
                                                                         ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  4:213609/132‑1 (MQ=255)
                                                                         ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  3:213609/1‑132 (MQ=255)
                                                                         ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCcttt                                                           >  2:10710/1‑139 (MQ=255)
                                                                         ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                            >  6:163849/1‑138 (MQ=255)
                                                                         ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                            >  7:88983/1‑138 (MQ=255)
                                                                         ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAAGGTCTCTTTTACATGTCCGATCGCCCACAGGCCGCTCTCATATTCTTTTTTATAGAGCGCcttt                                                           >  3:29375/1‑139 (MQ=255)
                                                                         ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGACCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTATTTATATAGCGCctt                                                            >  3:273095/1‑138 (MQ=255)
                                                                           ccGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATa                                                                                 >  5:19108/1‑115 (MQ=255)
                                                                           ccGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATa                                                                                 <  6:19108/115‑1 (MQ=255)
                                                                            cGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        <  6:158547/75‑1 (MQ=255)
                                                                            cGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        >  5:158547/1‑75 (MQ=255)
                                                                                  catcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATg                                                   <  1:80276/138‑1 (MQ=255)
                                                                                    tcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgcag                                                >  4:257760/1‑139 (MQ=255)
                                                                                    tcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGCCAGAGACACGCCCAATGTCTCTTTTACATGTACGATCGCCCACAGGACGATCTCATATTCTTTTTTATAACGCCACTTTAACTCATccag                                                >  7:33478/1‑139 (MQ=255)
                                                                                             tATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAtt                                       >  1:241593/1‑139 (MQ=255)
                                                                                             tATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAt                                        >  5:98707/1‑138 (MQ=255)
                                                                                             tATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAt                                        >  6:225908/1‑138 (MQ=255)
                                                                                             tATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAt                                        >  7:45120/1‑138 (MQ=255)
                                                                                              aTGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCATTGTCTCTTTTACATGTCCGAt                                                                                                      <  7:87463/75‑1 (MQ=255)
                                                                                              aTGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        >  8:44280/1‑57 (MQ=255)
                                                                                              aTGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        <  7:44280/57‑1 (MQ=255)
                                                                                              aTGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGAt                                                                                                      >  8:87463/1‑75 (MQ=255)
                                                                                                   tgagAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  3:232582/103‑1 (MQ=255)
                                                                                                   tgagAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  4:232582/4‑106 (MQ=255)
                                                                                                    gtgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGcac                                 >  6:12071/1‑138 (MQ=255)
                                                                                                     tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  6:145128/1‑104 (MQ=255)
                                                                                                     tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  5:145128/104‑1 (MQ=255)
                                                                                                       aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAagc                             <  2:182910/139‑1 (MQ=255)
                                                                                                       aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAagc                             >  6:155512/1‑139 (MQ=255)
                                                                                                          ggCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAagcagc                          <  4:117428/139‑1 (MQ=255)
                                                                                                           gCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                         <  3:240823/139‑1 (MQ=255)
                                                                                                           gCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                         <  5:75987/139‑1 (MQ=255)
                                                                                                            cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        >  6:287485/1‑43 (MQ=255)
                                                                                                            cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGt                                                                                                                        <  5:287485/43‑1 (MQ=255)
                                                                                                            cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                         >  1:252645/1‑138 (MQ=255)
                                                                                                               tataACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCAtt                     >  5:285108/1‑139 (MQ=255)
                                                                                                               tataACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTGTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCttt                     >  2:261027/1‑139 (MQ=255)
                                                                                                                    cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                               <  2:102605/76‑1 (MQ=255)
                                                                                                                    cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATAtt                                                                               >  1:102605/1‑76 (MQ=255)
                                                                                                                    cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  <  7:158691/89‑1 (MQ=255)
                                                                                                                    cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATagag                                                                  >  8:158691/1‑89 (MQ=255)
                                                                                                                    cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                         <  3:212656/130‑1 (MQ=255)
                                                                                                                    cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                         >  4:212656/1‑130 (MQ=255)
                                                                                                                    cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGaa                >  2:33684/1‑139 (MQ=255)
                                                                                                                    cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGaa                >  2:169932/1‑139 (MQ=255)
                                                                                                                         ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAgcgcct                                                             <  3:65618/89‑3 (MQ=255)
                                                                                                                         ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAgcgcct                                                             >  4:65618/1‑87 (MQ=255)
                                                                                                                         ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGATCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt           >  8:201648/1‑139 (MQ=255)
                                                                                                                                 cAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCg    <  2:241593/138‑1 (MQ=255)
                                                                                                                                  aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa  <  7:26819/139‑1 (MQ=255)
                                                                                                                                  aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa  <  3:8114/139‑1 (MQ=255)
                                                                                                                                  aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa  <  6:285108/139‑1 (MQ=255)
                                                                                                                                  aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa  <  5:225908/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCA  >  CP006881/3724259‑3724527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑