| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,837,361 | A→C | 26.2% | F34C (TTT→TGT) | U712_19795 ← | Uncharacterized protein yxiK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,837,361 | 0 | A | C | 26.2% | 79.8 / 13.5 | 48 | F34C (TTT→TGT) | U712_19795 | Uncharacterized protein yxiK |
| Reads supporting (aligned to +/- strand): ref base A (33/2); new base C (2/11); total (35/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCCCTTTTGGCCAGCTTCGTCACCTTGTAAGCATGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATAGCGCAACG > CP006881/3837227‑3837495 | aTTCCCTTTTGGCCAGCTTCGCCACCTTGAAAGCATGCCATACAAAAAACGCGCACAGGTCTTATAAAAATCACCCCGTCTTTATACCCTGTACCATGATGCGATCGTCGGCACGCCCTCCCAAAAAA‑C‑GATAAAACGa < 8:216854/139‑1 (MQ=255) ttttGGCCAGCTTCGTCACCTTGTAAGCATGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAAAGACTATg > 7:209946/1‑138 (MQ=255) cTTCGTCACCTTGTAAGCATGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGt > 3:45475/1‑139 (MQ=255) tCACGTTGTACCCATGACATTAAAAAAACTCCGACAGGTTTTATAAAAATCACCCAGTGTTTATTACGTTTAAAATG‑TTCCTTCCGACGCACCCCCCTCCCAAAAACC‑TAAAACACGACTCTGCATCTTCAAGTTCGCa < 8:156220/139‑1 (MQ=255) tGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTTTTACCTGTTTAATGATTGGATCGTATGGAAGCCCAGCCCAAAAA‑C‑GATAAAACGACTCTGGATCTTCAACTTCGCATGCCAAAACGGttt > 3:179667/1‑139 (MQ=255) tGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGttt > 7:223260/1‑139 (MQ=255) tGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTAACATTATTCGATCGTAGGCAAGCCAGCCCCAAAAA‑C‑AAAAACACCACTATGCAAATTAAAGTTCGAATGCCATAACCGttt > 4:264557/1‑139 (MQ=255) gACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTg > 8:196243/1‑139 (MQ=255) cTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTAACTGTATAATGATTAGATCGTAGGCAAGCCCGGACCCAAAA‑C‑GCTAAAAAGACAGTGCATCGTCAATTTCGCATGCCAATACAGTTTGGTCAGGCTTAAGc > 6:296323/1‑139 (MQ=255) ggACAGGTCTTCTATAAATGAACCAGTCGTTATTTCCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAACTTCGCATGCCATAACGGTTTGGTCAGGCTGAAGcgc > 8:95722/1‑136 (MQ=255) ggACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCAGCATCTTCAACTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCg > 5:237076/1‑139 (MQ=255) aCAGGTCTTATAAAAATCAACCCGTCTTTATTACCTTTATCATTATGCGATCTTCGGCACGCCCTGCCAAAAAA‑C‑TAAAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGAt < 7:293086/139‑1 (MQ=255) aataAATGAACCAGTCGTTATTACCTTTATCATTATGCGATCGTAGGCAACCCCTGCCCAAAAA‑C‑TAAAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCaa < 8:223260/137‑1 (MQ=255) aaaaTCAACCAGTCTTAATAACCTGTATCATGATTCGATCGTAGCCAACCCCTCCCAAAAAA‑C‑CAAAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCaa < 3:124645/135‑1 (MQ=255) aaTGAACCAGTCGTTATTTCCTGTATCATTAATCGATCGTAGGCAAGACAAGCCCAAAAA‑A‑GATAAAACGACTCTGCATCTTCAAGTTCGCTATCCAGAACCGTTTTGTAAGGCTTTAGCTCGATCATTTACAAATTGc > 2:203268/1‑139 (MQ=255) aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGc > 5:211832/1‑139 (MQ=255) aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGc > 5:127628/1‑139 (MQ=255) aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGc > 4:169920/1‑138 (MQ=255) gacatgTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCaa > 5:15202/6‑138 (MQ=255) aCCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATa > 6:109338/1‑139 (MQ=255) cggcGTTATTACCTGTATCATGTTGCGAGCGTCGGCAAGCCCGGCCAAAAAA‑C‑TAAAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTg < 7:98161/136‑1 (MQ=255) gTCGTTATTTCCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCCTCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGc > 7:252540/1‑139 (MQ=255) gTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACACGGCATCTTCAAGTTCGCAAGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCAAGTCCAAATTGCCAATACTGCTta > 7:203682/1‑139 (MQ=255) gTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTAt > 7:109695/1‑139 (MQ=255) tATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCACGTGCGCATGCCATATCGGGTGGGTCAGGCTTAAGCTCGCTCATGTCCAACTTGCCAATCCTGCTTATCGAGGTTAtt > 6:49694/1‑139 (MQ=255) tATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATATAGGTTAtt > 7:252692/1‑139 (MQ=255) tATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTAtt > 3:275013/1‑139 (MQ=255) aTCTTGATTCGAGCGTAGCCACCCCCGCCCAAAAAA‑C‑CAAAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTAtt < 4:275013/138‑1 (MQ=255) gATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTACCAATACTGCTTATAGAGGTTATTTCCAt > 3:209693/1‑138 (MQ=255) aTTCGTTCTTAGCCACGCGCTCCCAAAAAA‑G‑AATAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATa < 4:21646/138‑1 (MQ=255) aTTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATa > 2:12296/1‑138 (MQ=255) aTTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATa > 8:46403/1‑138 (MQ=255) aTCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAAtat > 2:250237/1‑137 (MQ=255) aTCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCt > 6:68285/1‑139 (MQ=255) aTCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCt > 4:10617/1‑139 (MQ=255) aTCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGCTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCt > 6:212957/1‑139 (MQ=255) ggCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCt > 5:267047/1‑139 (MQ=255) ggCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTAGTTCCATAAAATCTTCCCCt > 8:83131/1‑139 (MQ=255) gCATGCCCTCCCCAAAAA‑C‑TAAAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCtt < 8:203682/139‑1 (MQ=255) acgCCCTCCCAAAACA‑C‑TAAAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTAt < 7:265943/137‑1 (MQ=255) cccTGCCCAAAAA‑C‑TAAAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGATGTTATTTCCATAAAATCTTCCCCTTATCAg < 2:79844/139‑1 (MQ=255) cccTGCCCAAAAA‑C‑GAAAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAg < 5:109338/139‑1 (MQ=255) cccAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa > 7:1488/1‑139 (MQ=255) cccAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa > 2:233893/1‑139 (MQ=255) cccAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa > 1:230394/1‑139 (MQ=255) ccAAAAA‑CATATAACACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGa < 6:76944/139‑1 (MQ=255) aTAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATAGcgcaacg > 1:121155/1‑139 (MQ=255) aTAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATAGcgcaacg > 5:130486/1‑139 (MQ=255) | ATTCCCTTTTGGCCAGCTTCGTCACCTTGTAAGCATGACCTTCAAAATACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑C‑GATAAAACGACTCTGCATCTTCAAGTTCGCATGCCATAACGGTTTGGTCAGGCTTAAGCTCGATCATGTCCAAATTGCCAATACTGCTTATAGAGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGATAGCGCAACG > CP006881/3837227‑3837495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |