BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,735	A→T	9.3%	intergenic (+20/‑136)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,735	0	A	T	9.3%	5272.5 / 147.8	1830	intergenic (+20/‑136)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (98/1540); new base T (180/11); total (279/1551)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1866 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3597‑3872

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTg                                                                                                                                     >  6:103852/1‑139 (MQ=255)

       tCATTTTCATAAATAAAAGAAACAAGACAAACTAAACAATTTAAGTACCATTACTTAAGAGCAAGTAATGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                    <  5:246234/139‑1 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAACTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                    <  7:220404/139‑1 (MQ=255)

        cAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                   <  5:296573/139‑1 (MQ=255)

         aGTTTCATCACGAAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGa                                                                                                                                   <  1:93951/138‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                   <  8:6034/138‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                   <  8:249293/138‑1 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                             <  6:150147/138‑1 (MQ=255)

               atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                             <  6:211391/138‑1 (MQ=255)

                tcaCGCAACGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  1:277297/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  7:173936/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  3:250187/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  6:87310/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  2:228877/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  4:143607/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  1:20894/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  3:293008/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  1:191366/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  3:230386/139‑1 (MQ=255)

                        tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTAAATTTAAACATTaaa                                                                                                                    >  4:180726/1‑136 (MQ=255)

                          aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                 <  4:93384/139‑1 (MQ=255)

                           aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                 <  3:66481/138‑1 (MQ=255)

                              acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                             <  3:85539/139‑1 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTATAATTTTAAAATTTCACCTTAATTAACGGaa                                                                                                            >  4:223222/1‑139 (MQ=255)

                               cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  1:229180/139‑1 (MQ=255)

                                gCCAAAGTAAACAATTTAAGTACAAATACTTATGATCAAGTATAGTCTATTTATAATAGTTATCTATTATTAAACGCGAGGAAATAATTCTATGAGTCGA‑TTTTTTAAATTTTTAAAATTAAACCAAACTaaaataaa                                                                                                            >  8:21358/1‑133 (MQ=255)

                                    aaGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTAGACGGTACTAAAGGGAATGgag                                                                                                       >  5:278329/1‑139 (MQ=255)

                                     aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                      <  5:80446/139‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                     <  2:285299/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  1:173453/139‑1 (MQ=255)

                                        aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  1:285145/138‑1 (MQ=255)

                                        aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  3:276971/138‑1 (MQ=255)

                                        aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  4:207012/138‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                  <  2:158009/139‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                    <  1:260666/137‑1 (MQ=255)

                                          aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAGTTAGACCGTACTTAAGGGGATGGAGATAAat                                                                                                 >  1:48856/1‑139 (MQ=255)

                                           cAAATTAAGTACCATTACTTAAGAGCAAGTATAGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                <  6:235325/139‑1 (MQ=255)

                                             aTTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattat                                                                                              <  7:8409/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  6:12369/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  3:99127/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  2:203720/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  3:201073/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  5:199966/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:50922/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  8:199955/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:283722/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:248709/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTAGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  8:272540/139‑1 (MQ=255)

                                              attAAGTAACATTACATATGAGCAAGTAATGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  1:145523/138‑1 (MQ=255)

                                               ttAAGTACGATTACTTATTAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAAGTTACACGTTACTATAGGGAATGTAGATAAattatt                                                                                             <  8:152149/138‑1 (MQ=255)

                                                tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAAttatta                                                                                            <  7:99421/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                           <  7:108987/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                           <  7:198226/138‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTGGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  2:17761/139‑1 (MQ=255)

                                                     aCCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAAAAATTCTATGAGGCGC‑TTTTTTAAATTTGGCAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                      <  7:126753/139‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                               <  4:123839/139‑1 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTGGTTAGTTACACGGTAATAAAGTGAATGGTGATAAAATATTATATTtaataatta                                                                               >  1:12049/1‑135 (MQ=255)

                                                             ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  6:53685/139‑1 (MQ=255)

                                                               aTGAGCAAGTATTGTCTATTTTTTATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTATATTTGGAAAGTTACACGTTACTAAATGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  8:283206/137‑1 (MQ=255)

                                                                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTATATTTTGAAAGTTTAACGTTTCTTAATGGTATTTAGATAAATTTTTATATATAATACTGAAAGCt                                                                          >  5:131553/1‑139 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  5:41749/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTTTTATTTTCTATGTT‑CTAAAAGGAATGGTGTTTAATTTTTATATATACTACTGAAAGcgtac                                                                       >  3:157160/1‑132 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  4:43887/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  5:228501/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  5:111649/139‑1 (MQ=255)

                                                                           tGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGATATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                 <  2:25160/138‑1 (MQ=255)

                                                                             tCTATTTTTAATAGTTATCTATTATTTAATGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGAAAGTTAAACGGTAAGAAAGGGGATGGTGATAAATTTTTTGTTATACAACTGGCCGCTTCCAAGAAGCt                                                               >  2:8321/1‑138 (MQ=255)

                                                                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAAGTTTAACGTTAATAAAGGGTATTGAGATTAATTATTATATTTTATAATGACAGCTTACAATAAGCt                                                               >  3:220845/1‑138 (MQ=255)

                                                                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAGTTAAACGTTTCTAAAGGGAATGGGGATTATTTTTTTTATATACTACTGCCAGCTTGCAAGAAGCt                                                               >  8:170513/1‑138 (MQ=255)

                                                                                agttttAATTGTTATATATTATTTAAAGGGAGGAAATAATTCTATTAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  5:178719/136‑1 (MQ=255)

                                                                                 tttttAATATTTATCTATTATTTAAAGTTATGAAATAATTATATGAGTATC‑TTTTTTAAATTTTGAAAGTTACACGTTAATAAATTTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                           <  2:129535/138‑1 (MQ=255)

                                                                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  5:262511/137‑1 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTTATTTTAACGTTAATAAAGGGAATTGTGAAAAATTATTTTATATAATAATGACACCATACAAGAATCTAAaaag                                                         >  2:54923/1‑139 (MQ=255)

                                                                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAgc                                                <  7:33674/139‑1 (MQ=255)

                                                                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTAAATTTTGAAAATTTAACGTTACAAAATGGAATGGAGATAAATTATTAGATATAATAATTACAGCTTCCAAGAAGCTAAATAGGTCCCTAgc                                                >  7:232948/1‑139 (MQ=255)

                                                                                             aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAgcg                                               <  1:161236/138‑1 (MQ=255)

                                                                                                  ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACg                                         <  5:213335/139‑1 (MQ=255)

                                                                                                       tAACTGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                     <  3:130868/138‑1 (MQ=255)

                                                                                                       tAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑GAAAGTTAAACGTTACTAAAGGGAATTGAGATAAATTATTATATATAATAAAGACAGCTTAAAAGAAACTAAAGACTTACCAAGCGCCGAAGGGGaa                                    >  8:77075/1‑139 (MQ=255)

                                                                                                       tAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                     <  4:278507/138‑1 (MQ=255)

                                                                                                                  aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                         <  1:73144‑M1/139‑8 (MQ=255)

                                                                                                                         cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                   <  7:67498‑M1/138‑14 (MQ=255)

                                                                                                                            ttagtGGC‑TTTTTTAAATTTGGAATGTTACACGTTACTAAAGGGAATGGTGATAAATTATTAGATATACTACTGACACCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  3:43181‑M1/137‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGCGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGACCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  4:251799‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:164932‑M1/139‑18 (MQ=255)

                                                                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  3:112412‑M1/139‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  8:120751‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  5:152470‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  4:70494‑M1/138‑18 (MQ=255)

                                                                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACAAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  5:61103‑M1/138‑18 (MQ=255)

                                                                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  8:35491‑M1/139‑20 (MQ=255)

                                                                                                                                      tttttAAATTTGTAATGTTATATGTTACTAAAGTTAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  8:231008‑M1/138‑26 (MQ=255)

                                                                                                                                      tttttAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  7:150347‑M1/138‑26 (MQ=255)

                                                                                                                                      tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagtgtat       <  6:296878‑M1/138‑26 (MQ=255)

                                                                                                                                      tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  6:188555‑M1/138‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  6:260037‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATTGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  1:100052‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGACGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  3:180776‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACCGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat       <  7:272649‑M1/137‑26 (MQ=255)

                                                                                                                                           aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta  <  7:209819‑M1/138‑31 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3597‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`